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A New Method for Blood NT-proBNP Determination Based on a Near-infrared Point of Care Testing Device with High Sensitivity and Wide Scope

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Biomedical and Environmental Sciences 2017年第6期30卷 426-431页

作者： ZHANG xiao guang SHU Yao Gen GAO Ju WANG Xuan LIU Li Peng WANG Meng cao Yu xi ZENG Yi National lnstitute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China Beijing Runbio Biotechnology Development Co. Ltd Beijing 100106 China No.2 People's Hospital of Dali City Dali 671003 Yunnan China Department of Laboratory Medicine Beijing Hospital National Center of Gerontology Beijing 100730 China

Objective To develop a rapid, highly sensitive, and quantitative method for the detection of NT-proBNP levels based on a near-infrared point-of-care diagnostic （POCT） device with wide scope. Methods The lateral flow assay （LFA） strip of NT-proBNP was first prepared to achieve rapid detection. Then, the antibody pairs for NT-proBNP were screened and labeled with the near-infrared fluorescent dye Dylight-800. The capture antibody was fixed on a nitrocellulose membrane by a scribing device. Serial dilutions of serum samples were prepared using NT-proBNP-free serum series. The prepared test strips, combined with a near-infrared POCT device, were validated by known concentrations of clinical samples. The POCT device gave the output of the ratio of the intensity of the fluorescence signal of the detection line to that of the quality control line. The relationship between the ratio value and the concentration of the specimen was plotted as a work curve. The results of 62 clinical specimens obtained from our method were compared in parallel with those obtained from the Roche E411 kit. Results Based on the log-log plot, the new method demonstrated that there was a good linear relationship between the ratio value and NT-proBNP concentrations ranging from 20 pg/mL to 10 ng/mL. The results of the 62 clinical specimens measured by our method showed a good linear correlation with those measured by the Roche E411 kit. Conclusion The new LFA detection method of NT-proBNP levels based on the near-infrared POCT device was rapid and highly sensitive with wide scope and was thus suitable for rapid and early clinical diagnosis of cardiac impairment.

关键词： NT-proBNP Near-infrared POCT device Lateral flow assay

Rice gene expression profiles responding to larval feeding of the striped stem borer at the 1st to 2nd instar stage

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Insect Science 2011年第3期18卷 273-281页

作者： Yang Sun Yong-Jun Zhang guang-Chun cao Shao-Hua Gu Kong-Ming Wu xi-Wu Gao Yu-Yuan Guo State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing College of Agriculture and Biotechnology China Agricultural University Beijing China

The objective of this study was to identify rice genes that are in response to the striped stem borer （SSB）（Chilo suppressalis Walker） feeding at the first to second larval stage. Using combined suppression subtractive hybridization （SSH） and dot blot approaches, we analyzed the induced defense genes that took place during the first 72 h of infesting intact rice （Oryza sativa L.） plants in sheath tissues with SSB larvae. By sequencing the whole SSH library, 39 expressed sequence tags involved in disease stress, insect stress or other stress responses were identified to be up-regulated by SSB larvae feeding. Among these genes, rice allene oxide cyclase （AOC）, terpene synthase （TPS） and four proteinase inhibitor （PI） genes were up-regulated by SSB larvae feeding. Real-time quantitative reverse transcription polymerase chain reaction analysis showed that four rice PI genes were already up-regulated at 6 h, and reached peaks between 6 h to 12 h. In addition, the transcription ofgene involving in jasmonate signaling pathway such as allene oxide cyclase （AOC） concerning rice early defense response to SSB feeding was activated after rice feeding by SSB for 2 h. Although the expression office terpene synthase （TPS） gene, involved in the biosynthesis ofmonoterpenes or diterpenes, was already up-regulated at 7 h, a significant increase in the expression was delayed until 12 h and reached its peak at 24 h. The present study identified six SSB-response genes and their expression patterns, which provides evidence and information to understand insect stress-response in plants.

关键词： allene oxide cyclase (AOC) proteinase inhibitor (P]) real-time quantitative PCR (RT-qPCR) striped stem borer (SSB) suppression subtractive hybridization (SSH) terpene synthase (TPS)

The Binding Characterization of Cry Insecticidal Proteins to the Brush Border Membrane Vesicles of Helicoverpa armigera, Spodoptera exigua, Spodoptera litura and Agrotis ipsilon

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Journal of Integrative Agriculture 2013年第9期12卷 1598-1605页

作者： LU Qiong cao guang-chun ZHANG Li-li LIANG Ge-mei GAO xi-wu ZHANG Yong-jun GUO Yu-yuan State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural Sciences College of Agriculture and Biotechnology China Agricultural University

Cry toxins produced by Bacillus thuringiensis （Bt） are effective biological insecticides against certain insect species. However, there are potential risks of the evolved resistance of insects to Cry toxin owing to decreased binding of toxins to target sites in the brush border membranes of the larva midgut. The Cry toxins with different binding sites in the larval midgut have been considered to be a good combination to deploy in delaying resistance evolution. Bioassay results demonstrated that the toxicity of different Cry toxins ranked differently for each species. The toxicity ranking was CrylAc〉CrylAb〉Cry2Ab for Helicoverpa armigera, CrylB〉CrylC〉Cry2Ab for Spodoptera exigua, and Cry2Ab〉CrylB〉 CrylC for S. litura. Only Cry2Ab was toxic to Agrotis ipsilon. Binding experiments were performed with ^125I-CrylAb, ^125I-CrylAc, ^125I-CrylB, ^125I-CrylC, ^125I-Cry2Ab and the brush border membranes vesicles （BBMV） from H. armigera, S. exigua, S. litura and A. ipsilon. The binding of CrylAb and CrylAc was shown to be saturable by incubating with increasing concentrations of H. armigera BBMV （Kd=（45.00＋2.01） nmol L-1 and （12.80＋0.18） nmol L^-1, respectively; Bmax=（54.95±l.79） ng and （55.44±0.91） ng, separately）. The binding of CrylB was shown to be saturable by incubating with increasing concentrations ofS. exigua BBMV （Kd=（23.26±1.66） nmol L^-1; Bmax=（65.37±1.87） ng）. The binding of ^125I-Cry toxins was shown to be non-saturable by incubating with increasing concentrations of S. litura and A. ipsilon BBMV. In contrast, CrylB and CrylC showed some combination with the BBMV orS. litura, and a certain amount of Cry2Ab could bind to the BBMV ofA. ipsilon. These observations suggest that a future strategy could be devised for the focused combination of specific cry genes in transgenic crops to control target pests, widen the spectrum of insecticide effectiveness and postpone insect resistance evolution.

关键词： Cry toxin toxicity binding assays 1251 labeling BBMV

Effect of dose rate on the characteristics of dislocation loops in palladium:In-situ TEM analysis during 30 keV H_(2)^(+)irradiation

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Journal of Materials Science & Technology 2023年第19期150卷 86-95页

作者： Dewang Cui Ziqi cao Yifan Ding Yipeng Li guang Ran College of Energy Xiamen UniversityXiamen 361102China Fujian Research Center for Nuclear Engineering Xiamen 361102China

Ion irradiation is usually used to simulate neutron irradiation to accelerate the evaluation of the irradia-tion behavior of reactor ***,the validity of using a high damage rate of ion irradiation to simulate a low damage rate of neutron irradiation has always been a controversial ***,the effect of two dose rates(2.94×10^(-6) and 7.35×10^(-5) dpa s^(-1))on the characteristics and evolution of dislo-cation loops in palladium was studied in situ during 30 keV H2+irradiation using transmission electron *** dose rate obviously affected the nucleation rate and growth rate of dislocation loops,the types(Frank loops or perfect loops)of dislocation loops,and the irradiation hardening and total damage obtained from the product of average loop size and loop *** the same irradiation dose,a high dose rate would lead to high loop density,small average loop size,low loop growth rate,and low irra-diation hardening and damage induced by loops in pure ***,it was found for the first time that a high dose rate was beneficial to the generation of perfect dislocation *** effect of dose rate was attributed to the different dynamic equilibrium results between the effective generation rate of point defects and their absorption rate by existing *** present results show that the effect of dose rate should be considered when using ion irradiation to simulate neutron irradiation to evaluate the irradiation damage to materials.

关键词： Palladium Hydrogen irradiation In-situ TEM observation Dislocation loop Dose rate

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企业数字化转型与全球价值链参与

管理工程学报 2025年第1期39卷 15-31页

作者：李鑫佟岩曹丰北京理工大学管理学院湖南大学工商管理学院

本文立足“逆全球化”时代数字经济发展迅速的现实背景,利用2010年至2016年中国A股上市公司与中国海关数据库的合并数据,实证检验了企业数字化转型对其全球价值链参与的影响。研究表明,数字化转型能够促进企业更好地融入全球价值链,并... 详细信息

本文立足“逆全球化”时代数字经济发展迅速的现实背景,利用2010年至2016年中国A股上市公司与中国海关数据库的合并数据,实证检验了企业数字化转型对其全球价值链参与的影响。研究表明,数字化转型能够促进企业更好地融入全球价值链,并且该结论在经过一系列稳健性检验后依然成立。影响机制检验发现,企业数字化转型对全球价值链嵌入的提升作用主要通过缓解融资约束和提高生产效率来实现。异质性检验结果表明,当数字化转型基础较好、劳动力需求较高以及成本加成较低时,企业数字化转型对全球价值链参与的影响更为凸显。本文丰富和拓展了企业数字化转型影响机制与经济后果的研究框架,为企业更好地融入全球价值链分工体系、实现高质量发展找到了新的经验证据,对于企业数字化相关政策的制定与调整也具有一定的参考价值。

关键词：数字化转型全球价值链嵌入融资约束生产效率

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Coupled Cross-Flow/in-Line Vortex-Induced Vibration Responses of a Catenary-Type Riser Subjected to Uniform Flows

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Journal of Ocean University of China 2024年第5期23卷 1202-1212页

作者： LI xiaomin cao xi LI Fuheng YANG Zhiwen College of Engineering Ocean University of ChinaQingdao 266404China College of Hydrodynamic Engineering Tianjin Research Institute for Water Transport EngineeringM.O.TTianjin 300456China

A three-dimensional numerical scheme was developed to investigate the vortex-induced vibration(VIV)of a catenary-type riser(CTR)in the in-line(IL)and cross-flow(CF)*** using the vector form intrinsic finite element method,the CTR was discretized into a finite number of spatial particles whose motions satisfy Newton’s second *** Van der Pol oscillator was used to simulate the effect of vortex *** coupling equations of structural vibration and wake oscillator were solved using an explicit central differential *** numerical model was verified with the published *** VIV characteristics of the CTR subjected to uniform flows,including displacement,frequency,standing wave,traveling wave,motion trajectory,and energy transfer,were studied *** numerical results revealed that the multimode property occurs in the CF-and IL-direction VIV responses of the *** increase in the flow velocity has slight effects on the maximum VIV *** to structural nonlin-earity,the double-frequency relationship in the CF and IL directions is rarely ***,the vibration trajectories display the shape of an inclined elliptical ***,the negative energy region is inconspicuous under the excitation of the uniform flow.

关键词： vortex-induced vibration vector form intrinsic finite element catenary-type riser wake oscillator equation

Origins of intraseasonal rainfall variations over the southern South China Sea in boreal winter

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Atmospheric and Oceanic Science Letters 2017年第1期10卷 44-50页

作者： cao xi WU Ren-guang Center for Monsoon System Research Chinese Academy of Sciences State Key Laboratory of Numerical Modeling for Atmospheric Sciences and Geophysical Fluid Dynamics Center for Monsoon System Research Institute of Atmospheric Physics Chinese Academy of Sciences Beijing China State Key Laboratory of Numerical Modeling for Atmospheric Sciences and Geophysical Fluid Dynamics Institute of Atmospheric Physics Chinese Academy of Sciences Beijing China

This study investigates the origins of intraseasonal rainfall variations over the southern South China Sea（SCS） region in boreal *** is found that intraseasonal rainfall variations over the southern SCS have different origins on the 10-20-day and 30-60-day time *** the 10-20-day time scale,large rainfall anomalies over the southern SCS are preceded by strong northerly wind anomalies associated with the East Asian winter monsoon（EAWM）,by about two *** the 30-60-day time scale,the strong EAWM-related northerly wind anomalies almost appear simultaneously with large rainfall anomalies over the southern *** addition,obvious large rainfall anomalies occur over the southeastern tropical Indian Ocean about one week before the peak southern SCS rainfall *** indicates that the convection and related circulation anomalies with origins over the tropical Indian Ocean may play an important role in inducing intraseasonal rainfall variations over the southern SCS on the 30-60-day time scale,but not on the 10-20-day time scale.

关键词： Intraseasonal rainfall anomalies southern South China Sea boreal winter 10-20-day and 30-60-day time scales

K-groups of Toeplitz algebras on connected domains

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Science China Mathematics 2007年第1期50卷 73-80页

作者： guang-fu cao xiao-hong WANG School of Mathematics and Information Sciences Guangzhou UniversityGuangzhou 510006China Department of Mathematics Sichuan UniversityChengdu 610064China

In the present paper, it is proved that the K0-group of a Toeplitz algebra on any connected domain is always isomorphic to the K0-group of the relative continuous function algebra. In addition, the cohomotopy groups of essential boundaries of some connected domains are computed, and the K0-groups of the continuous function algebras on these domains are also computed.

关键词： essential boundary cohomotopy group K-group 46L80 47B35

Coalition Formation in Weighted Simple-majority Games under Proportional Payoff Allocation Rules

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International Journal of Automation and computing 2009年第3期6卷 217-222页

作者： Zhi-Gang cao xiao-guang Yang Key Laboratory of Management Decision and Information SystemsAcademy of Mathematics and Systems Science Chinese Academy of Sciences Beijing 100190 PRC

In this paper, we introduce a simple coalition formation game in the environment of bidding, which is a special case of the weighted majority game （WMG）, and is named the weighted simple-majority game （WSMG）. In WSMG, payoff is allocated to the winners proportional to the players powers, which can be measured in various ways. We define a new kind of stability： the counteraction-stability （C-stability）, where any potential deviating players will confront counteractions of the other players. We show that C-stable coalition structures in WSMG always contains a minimal winning coalition of minimum total power. For the variant where powers are measured directly by their weights, we show that it is NP-hard to find a C-stable coalition structure and design a pseudo-polynomial time algorithm. Sensitivity analysis for this variant, which shows many interesting properties, is also done. We also prove that it is NP-hard to compute the Holler-Packel indices in WSMGs, and hence in WMGs as well.

关键词： Weighted majority games (WMG) coalition formation bidding C-stability NP-hard.