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基于核壳金@铂纳米粒子的Ag^+比色检测方法

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分析测试学报 2016年第11期35卷 1496-1499,1504页

作者：陈铭铭吴亮亮谢正军彭池方江南大学食品学院食品科学与技术国家重点实验室江苏无锡214122

金核铂壳纳米粒子(Au@Pt NPs)具有出色的类过氧化物酶活性,而Ag^+对其催化活性表现出强烈的抑制;基于此,构建了高灵敏的Ag^+比色检测方法。在最佳反应条件下,Au@Pt NPs比色检测Ag^+的线性范围为0.1~10 nmol/L,检出限可达0.05 nmol/L。... 详细信息

金核铂壳纳米粒子(Au@Pt NPs)具有出色的类过氧化物酶活性,而Ag^+对其催化活性表现出强烈的抑制;基于此,构建了高灵敏的Ag^+比色检测方法。在最佳反应条件下,Au@Pt NPs比色检测Ag^+的线性范围为0.1~10 nmol/L,检出限可达0.05 nmol/L。该方法对汞离子(Hg2^+)也表现出高灵敏的响应,比色检测Hg2^+的线性范围为10~200 nmol/L。将其应用于实际水样中Ag^+的检测,在添加浓度为10,50,100 nmol/L时,回收率为83.8%~97.7%,相对标准偏差为3.0%~9.6%,该方法具有操作简单、灵敏度高、成本低等优点。

关键词： Au@Pt纳米粒子模拟酶比色检测银离子汞离子

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胎儿多发畸形与染色体异常的相关性分析

中山大学学报（医学科学版） 2011年第2期32卷 232-236页

作者：谢英俊方群吴坚柱陈宝江陈健生陈筠虹陈争中山大学附属第一医院胎儿医学中心广东广州510080 中山大学中山医学院医学遗传学研究室广东广州510080

【目的】探讨多发畸形胎儿的产前诊断特征及其与染色体异常的关系。【方法】对853例产前诊断胎儿进行研究,根据超声检测检出多发畸形与否,分为多发畸形组(n=103)及非畸形组(对照组,n=750),行常规染色体核型分析;收集相关临床资料:分析... 详细信息

【目的】探讨多发畸形胎儿的产前诊断特征及其与染色体异常的关系。【方法】对853例产前诊断胎儿进行研究,根据超声检测检出多发畸形与否,分为多发畸形组(n=103)及非畸形组(对照组,n=750),行常规染色体核型分析;收集相关临床资料:分析胎儿畸形超声发现时期、畸形类别、畸形数目、染色体异常率和异常类型以及染色体异常与多发畸形的相关性。【结果】两组胎儿比较,染色体异常率(多发畸形组43.69%,对照组0.93%),性别比(多发畸形组1.94,对照组0.97),产前诊断孕周[多发畸形组(25±5)周,对照组(21±4)周],差异均有统计学意义(P<0.05)。超声发现胎儿畸形数目越多,胎儿染色体异常的风险越大(r=0.792,P=0.017)。多变量统计学分析的结果提示超声检出胎儿的面颈部异常(OR=7.748,P=0.000)、心血管系统异常(OR=5.064,P=0.002)、泌尿系统畸形(OR=0.195,P=0.005)、单脐动脉(OR=4.608,P=0.020)与多发畸形胎儿的染色体异常相关。【结论】多发畸形胎儿染色体异常率高;在超声对多发畸形胎儿检测中,胎儿的面颈部异常、心血管系统异常、单脐动脉可能可以作为胎儿染色体异常的预测指标。

关键词：染色体异常多发畸形胎儿超声检查

Efficacy and safety of sirolimus early conversion protocol in liver transplant patients with hepatocellular carcinoma:A single-arm, multicenter, prospective study

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Hepatobiliary & Pancreatic Diseases International 2022年第2期21卷 106-112页

作者： Ren-Yi Su Sun-Bin Ling Qiao-Nan Shan Xu-Yong Wei Rui wang Chang-Ku Jia Li Zhuang Tian Shen Li-Min Ding Zhi-Dan Xu Lai-Bang Luo Li-Bo Sun Guang-Ming Li Tai-Shi fang Nan Jiang Kun Zhang Zhao-Jie Su Zhi-Hai Peng Ren Lang Tao Jiang Qiang He Lin-Sen Ye Yang Yang Yu-Ting He Wen-Zhi Guo Liu-Gen Lan Xu-Yong Sun Dong chen Zhi-Shui chen Da-Wei Zhou Shao-jun Ye Qi-Fa Ye Min Tian Jian-Hua Shi Bo wang Jiang Liu Qian Lu Wei Rao Jin-Zhen Cai Tao Lv Jia-Yin Yang Pu-Sen wang Lin Zhong Jing-Sheng Ma Qi-Gen Li Sheng-Dong wu Chang-Jiang Lu Cai-De Lu Dong-Hua Zhang Xuan wang Zi-Qiang Li Mu-Jian Teng jun-Jie Li Wen-Tao Jiang Jian-Hua Li Quan-Bao Zhang Ning-Qi Zhu Zheng-Xin wang Kang He Qiang Xia Shao-Hua Song Zhi-Ren Fu Wei Qiu Guo-Yue Lv Rui-Peng Song Ji-Zhou wang Zheng wang Jian Zhou Gang chen Ying-Peng Zhao Li Li Ze-Min Hu Qi-Jie Luo Zhong-Zhou Si Bin xie Xiao-Shun He Zhi-Yong Guo Shu-Sen Zheng Xiao Xu Department of Hepatobiliary and Pancreatic Surgery The Center for Integrated Oncology and Precision MedicineAffiliated Hangzhou First People’s HospitalZhejiang University School of MedicineHangzhou 310006China NHC Key Laboratory of Combined Multi-organ Transplantation Hangzhou 310003China Department of Hepatobiliary and Pancreatic Surgery Shulan(Hangzhou)HospitalZhejiang Shuren University School of MedicineHangzhou 310022China Department of Hepatobiliary and Pancreatic Surgery The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China Department of Transplantation Jiangxi Provincial People’s Hospital Affiliated to Nanchang UniversityNanchang 330006China Liver Transplantation Center Beijing You’an HospitalCapital Medical UniversityBeijing 100069China Department of Hepatic Surgery National Clinical Research Center for Infectious DiseaseShenzhen Third People’s HospitalThe Second Affiliated Hospital of Southern University of Science and TechnologyShenzhen 518112China Department of General Surgery Xiang’an Hospital of Xiamen UniversityXiamen 361000China Deartment of Hepatobiliary and Pancreaticosplenic Surgery Beijing Chaoyang HospitalCapital Medical UniversityBeijing 100020China Department of Hepatic Surgery and Liver Transplantation Center The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510630China Department of Hepatobiliary and Pancreatic Surgery The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China Department of Liver Transplantation The Second Affiliated Hospital of Guangxi Medical UniversityNanning 530000China Institute of Organ Transplantation Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Institute of Hepatobiliary Diseases of wuhan University Transplant Center of Wuhan UniversityHubei Key Laboratory of Medical Technology on TransplantationZhongnan Hospital of Wuhan UniversityWuhan 430071China Department of Hepatobiliary Surgery The First Affiliated Hos

Mammalian target of rapamycin(m TOR) inhibitor as an attractive drug target with promising antitumor effects has been widely investigated. High quality clinical trial has been conducted in liver transplant(LT) recipients in Western countries. However, the pertinent studies in Eastern world are paucity. Therefore, we designed a clinical trial to test whether sirolimus can improve recurrence-free survival(RFS) in hepatocellular carcinoma(HCC) patients beyond the Milan criteria after LT. This is an open-labeled, single-arm, prospective, multicenter, and real-world study aiming to evaluate the clinical outcomes of early switch to sirolimus-based regimens in HCC patients after LT. Patients with a histologically proven HCC and beyond the Milan criteria will be enrolled. The initial immunosuppressant regimens are center-specifc for the frst 4-6 weeks. The following regimens integrated sirolimus into the regimens as a combination therapy with reduced calcineurin inhibitors based on the condition of patients and centers. The study is planned for 4 years in total with a 2-year enrollment period and a 2-year follow-up. We predict that sirolimus conversion regimen will provide survival benefts for patients particular in the key indicator RFS as well as better quality of life. If the trial is conducted successfully, we will have a continued monitoring over a longer follow-up time to estimate indicator of overall survival. We hope that the outcome will provide better evidence for clinical decision-making and revising treatment guidelines based on Chinese population data.

关键词： Sirolimus Immunosuppressive agents Hepatocellular carcinoma Liver transplantation Conversion

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大熊猫双胞胎生长发育影响因素的研究

四川动物 2019年第4期38卷 380-386,401页

作者：王树群奉永友陈波吴孔菊方俊谢意黄祥明成都大熊猫繁育研究基地濒危动物繁殖与保护遗传省重点实验室

为了研究影响大熊猫Ailuropoda melanoleuca双胞胎生长发育的因素,以2013—2016年成都大熊猫繁育研究基地出生的22对大熊猫双胞胎幼仔及其母兽为研究对象,分析母兽孕期增重、双胞胎个体性别和双胞胎性别类型与大熊猫双胞胎幼仔初生体质... 详细信息

为了研究影响大熊猫Ailuropoda melanoleuca双胞胎生长发育的因素,以2013—2016年成都大熊猫繁育研究基地出生的22对大熊猫双胞胎幼仔及其母兽为研究对象,分析母兽孕期增重、双胞胎个体性别和双胞胎性别类型与大熊猫双胞胎幼仔初生体质量之间的关系,以及双胞胎个体性别和大熊猫幼仔初生体质量与1~12月龄大熊猫幼仔体质量之间的关系。结果显示,不同双胞胎性别类型的大熊猫幼仔初生体质量的差异有统计学意义(P=0.015),但母兽孕期增重、双胞胎个体性别与幼仔初生体质量的差异无统计学意义(P=0.257、P=0.170);双胞胎2只个体的初生体质量不同(χ^2=9.043,P=0.041),且双胞胎性别类型与幼仔初生体质量差异程度呈中等强度相关(Cramer s V=0.492,P=0.030),双雌性双胞胎初生体质量差异为20%~40%(调整后标化残差:2.30),双雄性双胞胎初生体质量差异小于20%(调整后标化残差:2.60);双胞胎个体性别和初生体质量对1~12月龄大熊猫幼仔生长发育的影响无统计学意义(P>0.05)。对大熊猫双胞胎幼仔生长发育影响因素的研究,为大熊猫双胞胎的饲养管理提供理论依据。

关键词：大熊猫双胞胎性别母兽孕期增重初生体质量

七例产前诊断的标记染色体及复杂染色体畸变的光谱核型分析

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中华医学遗传学杂志 2012年第4期29卷 393-397页

作者：宋花蕾陈宝江方群谢英俊林少宾吴坚柱山东省滨州医学院附属医院妇产科 256603 中山大学附属第一医院妇产科胎儿医学中心

目的探讨光谱核型分析（spectral karyotyping，SKY）结合荧光原位杂交（fluorescence in situ hybridization，FISH）及传统核型分析技术在产前诊断标记染色体及复杂染色体畸变中的应用。方法对产前诊断中常规G显带分析发现的5例标记... 详细信息

目的探讨光谱核型分析（spectral karyotyping，SKY）结合荧光原位杂交（fluorescence in situ hybridization，FISH）及传统核型分析技术在产前诊断标记染色体及复杂染色体畸变中的应用。方法对产前诊断中常规G显带分析发现的5例标记染色体以及2例复杂染色体畸变的胎儿样本进行SKY分析，必要时应用FISH技术进一步鉴定或采用C显带、N显带技术进行辅助诊断，并分析胎儿产前超声检查、生后随访或病理解剖结果。结果5例标记染色体的病例中2例为大的标记染色体，3例为中等大小标记染色体；1例胎儿为父源性遗传，4例为新发突变。用SKY分析发现2例为非近端着丝粒来源（分别为4号、9号染色体），2例为近端着丝粒来源（分别来自22号、21号染色体），1例为X染色体来源。3例经FISH检查证实了SKY分析结果。5例标记染色体的胎儿4例终止妊娠，1例父源性遗传者足月分娩，生后随访1年未见异常。2例产前诊断为复杂染色体畸变的胎儿，其中1例经常规G显带分析为不明来源的衍生染色体，SKY诊断为8号染色体自身部分重复；另1例经SKY诊断为2号与6号染色体易位的胎儿足月分娩，随访6个月时有生长发育迟缓。结论应用SKY结合FISH及传统的核型分析可对产前诊断中难以确定来源的标记染色体及复杂染色体畸变作出诊断，结合超声波检查结果，可更好地为临床咨询提供指导。

关键词：标记染色体染色体畸变光谱核型分析产前诊断

铜陵矿山酸性排水及固体废弃物中的重金属元素

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岩石矿物学杂志 2005年第6期24卷 591-597页

作者：徐晓春陈芳王军谢巧勤陆三明吴文涛陈天虎合肥工业大学资源与环境工程学院安徽合肥230009

在调查中国铜陵凤凰山铜矿和新桥硫铁矿两种不同类型矿山固体废弃物特征的基础上,研究了矿山尾矿和废石产生酸性排水的可能性及其差异以及矿山固体废弃物中重金属元素的赋存形式。结果表明,凤凰山铜矿的尾矿基本不产生矿山酸性排水,而... 详细信息

在调查中国铜陵凤凰山铜矿和新桥硫铁矿两种不同类型矿山固体废弃物特征的基础上,研究了矿山尾矿和废石产生酸性排水的可能性及其差异以及矿山固体废弃物中重金属元素的赋存形式。结果表明,凤凰山铜矿的尾矿基本不产生矿山酸性排水,而新桥硫铁矿采矿废石产生矿山酸性排水,并且凤凰山铜矿的尾矿和新桥硫铁矿采矿废石中重金属元素的赋存形式也有差异,前者重金属Cu、Pb、Zn、Cd、As、Hg主要赋存于硅酸盐态中,而后者在还原态中有较高的含量,这反映了在地表条件下尾矿中大量重金属元素已经发生了迁移,而采矿废石已经开始氧化,且酸性排水的存在更有利于废石中重金属元素的迁移和扩散,进而导致矿区周围环境的污染。

关键词：矿山酸性排水固体废弃物重金属污染中国铜陵

4种算法评估落地动作中ACL负荷的对比研究

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体育科学 2018年第3期38卷 73-79页

作者：黄尚军伍勰阮棉芳张强陈晨上海体育学院运动健身科技省部共建教育部重点实验室上海200438 上海中医药大学附属第七人民医院康复治疗科上海200137 宁波大学体育学院浙江宁波315211 苏黎世联邦理工学院生物力学研究所瑞士

目的:采用4种不同的算法模型估算落地动作过程中ACL动态载荷并进行对比分析,探讨造成差异的生物力学机制。方法:选择1名女性田径项目运动员为对象,同步采集40 cm高台垂直落地动作中的下肢运动学、动力学和表面肌电数据。4种算法模型及... 详细信息

目的:采用4种不同的算法模型估算落地动作过程中ACL动态载荷并进行对比分析,探讨造成差异的生物力学机制。方法:选择1名女性田径项目运动员为对象,同步采集40 cm高台垂直落地动作中的下肢运动学、动力学和表面肌电数据。4种算法模型及相应技术路线分别为:1)基于逆向动力学算法模型计算胫前剪切力(ATSF_1);2)基于Paul Devita数学模型计算胫前剪切力(ATSF_2);3)采用Open Sim CMC过程优化膝关节周围肌肉群肌力,代入Paul Devita数学模型重新计算胫前剪切力(ATSF_3);4)基于Julia Kar提供的Open Sim ACL模型计算ACL拉力(F_(ACL))。结果:Open Sim CMC结果与EMG信号在曲线形态有较高的一致性;4种计算方法下所对应的落地过程中,ATSF_1、ATSF_2、ATSF_3、F_(ACL)峰值分别为1.1BW、2.0BW、4.5BW、1.7BW;峰值时刻分别为落地周期的62%、80%、55%、36%。结论:基于传统逆向动力学算法的ATSF_1可能低估了运动过程中的ACL载荷,可量化肌群肌力的不同算法模型下ACL载荷结果的不同,主要归因于估算肌力方法的本身差异。

关键词：落地动作前交叉韧带载荷评估 OpenSim 模拟仿真

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利用皮秒脉冲激光驱动瞬态X射线激光

物理学报 2006年第8期55卷 4156-4160页

作者：王琛方智恒王伟熊俊孙今人吴江傅思祖顾援王世绩张国平郑无敌黄关龙管福义谢兴龙林尊琪上海激光等离子体研究所上海201800 北京应用物理与计算数学研究所北京100088 中国科学院上海光学精密机械研究所上海201800

报道了利用皮秒激光驱动产生瞬态类镍银X射线激光的实验结果.采用一路脉冲宽度为数百皮秒的激光作为预脉冲,配合另一路皮秒激光作为主脉冲联合驱动平面靶,获得了一定强度的类镍银X射线激光输出,输出能量约为5—10nJ.

关键词：瞬态X射线激光长短脉冲联合驱动皮秒脉冲激光

Prevalence of Acne Inversa(Hidradenitis Suppurativa)in China:A Nationwide Cross-Sectional Epidemiological Study

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International Journal of Dermatology and Venereology 2022年第1期5卷 1-7页

作者： Zhong-Shuai wang Jing Li Qiang Ju Wei Lai Song-Mei Geng Xiao-Jing Kang Xian Jiang Hong-Zhong Jin Hong-Fu xie Qing Sun Hang Li Shan-Shan Li chen Yuan Li He Hong fang Gang wang Juan Tao Xing-Hua Gao Yan wu You-Kun Lin Yu-Zhen Li jun Gu Yan Yan Baoxi wang Department of Dermatology Plastic Surgery HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100144China Institute of Dermatology Chinese Academy of Medical Sciences and Peking Union Medical CollegeNanjingJiangsu 210042China Department of Dermatology Renji HospitalShanghai Jiaotong University School of MedicineShanghai 200127China Department of Dermatology The Third Affiliated HospitalSun Yat-Sen UniversityGuangzhouGuangdong 510630China Department of Dermatology The Second Affiliated Hospital of Xi’an Jiaotong UniversityXi’anShaanxi 710004China Department of Dermatology People’s Hospital of Xinjiang Uygur Autonomous RegionUrumqiXinjiang 830001China Department of Dermatology West China HospitalSichuan UniversityChengduSichuan 610041China Department of Dermatology Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China Department of Dermatology Xiangya HospitalCentral South UniversityChangshaHunan 410008China Department of Dermatology Qilu Hospital of Shandong UniversityJinanShandong 250012China Department of Dermatology Peking University First HospitalBeijing 100034China Department of Dermatology the First Bethune Hospital of Jilin UniversityChangchunJilin 130021China Department of Dermatology First Affiliated Hospital of Kunming Medical UniversityKunmingYunnan 650032China Department of Dermatology the First Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiang 310003China Department of Dermatology Xijing HospitalAir Force Medical University of PLAXi’anShaanxi 710032China Department of Dermatology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430022China Department of Dermatology the First Hospital of China Medical UniversityShenyangLiaoning 110001China Department of Dermatology the First Affiliated Hospital of Guangxi Medical UniversityNanningGuangxi 530021China Department of Dermatology t

Objective:This study was performed to estimate the prevalence of hidradenitis suppurativa(HS)in China and describe the demographic and clinical features of HS in the Chinese ***:This multicenter clinic-based cross-sectional study was conducted in dermatology clinics within 19 hospitals located in 15 cities/provinces across China from September to October *** prevalence of HS was calculated as the percentage of patients with HS among all visitors at participating clinics during the 1-month study *** was independently diagnosed by two certified dermatologists at each *** visitors at participating clinics were screened,and all patients with HS were invited to participate in a questionnaire survey to elucidate the demographics and clinical features of HS in the Chinese ***:We identified 92 patients with HS among 274,742 visitors at participating *** prevalence rate was 0.03349%or 33.49 per 100,000 population(95%confidence interval,26.64–40.32),and the female:male ratio was 1.0:***:The overall prevalence of HS in China was lower than that in Western and other Asian countries with a predominance of male patients.

关键词： acne inversa/hidradenitis suppurativa epidemiology China