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基于道路交通噪声预防的规划指标研究

城市交通 2024年第3期22卷 85-91页

作者：邓惠章杨晔曹雪柠汪益纯王树盛章燕周进夏胜国陈宗军江苏省规划设计集团有限公司江苏南京210019

道路交通噪声已成为影响城市居民生活最大的环境污染源之一。为推动降噪型用地布局模式落地应用、推进宁静城市建设,针对既有规划指标体系尚未纳入噪声预防相关指标的现状,提出将空间噪声指数作为定量化的噪声预防规划指标。进而提出空... 详细信息

道路交通噪声已成为影响城市居民生活最大的环境污染源之一。为推动降噪型用地布局模式落地应用、推进宁静城市建设,针对既有规划指标体系尚未纳入噪声预防相关指标的现状,提出将空间噪声指数作为定量化的噪声预防规划指标。进而提出空间噪声指数的理想指标值计算模型,并结合拉萨、张家港等10个案例进行指标计算与评估。对于实际指标值偏大的案例,建议参考降噪型用地布局模式,以空间噪声指数为约束对用地空间布局进行优化调整。降噪型用地布局模式揭示了城市交通与土地利用之间的互动新机制,而借助空间噪声指数可对该机制进行客观评估。最后提出将空间噪声指数作为空间品质类约束指标纳入城市总体规划指标体系并向下传导落实,对中远期规划实践以及城市更新等近期治理行动进行约束、引导和评估。

关键词：道路交通噪声前端预防降噪型用地布局模式空间噪声指数规划指标宁静城市

High-throughput genome editing in rice with a virus-based surrogate system

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Journal of Integrative Plant Biology 2023年第3期65卷 646-655页

作者： Yifu Tian Dating Zhong Xinbo Li Rundong Shen Han Han Yuqin Dai Qi Yao xuening Zhang Qi Deng Xuesong cao Jian-Kang Zhu Yuming Lu Shanghai Center for Plant Stress Biology Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 201602China Center for Advanced Bioindustry Technologies Institute of Crop SciencesChinese Academy of Agricultural SciencesBeiing 100081China Hainan Yazhou Bay Seed Lab Sanya 572024China Shanghai Collaborative Innovation Center of Agri Seeds Joint Center for Single Cell BiologySchool of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China Institute of Advanced Biotechnology School of Life SciencesSouthern University of Science and TechnologyShenzhen 518055China

With the widespread use of clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated nuclease(Cas) technologies in plants, large-scale genome editing is increasingly needed. Here, we developed a geminivirus-mediated surrogate system, called Wheat Dwarf Virus-Gate(WDV-surrogate), to facilitate high-throughput genome ***-Gate has two parts: one is the recipient callus from a transgenic rice line expressing Cas9 and a mutated hygromycin-resistant gene(HygM) for surrogate selection;the other is a WDV-based construct expressing two single guide RNAs(sgRNAs) targeting HygM and a gene of interest, respectively. We evaluated WDV-Gate on six rice loci by producing a total of 874 T_0 plants. Compared with the conventional method, the WDV-Gate system, which was characterized by a transient and high level of sgRNA expression, significantly increased editing frequency(66.8% vs. 90.1%), plantlet regeneration efficiency(2.31-fold increase), and numbers of homozygous-edited plants(36.3%vs. 70.7%). Large-scale editing using pooled sg RNAs targeting the SLR1 gene resulted in a high editing frequency of 94.4%, further demonstrating its feasibility. We also tested WDVGate on sequence knock-in for protein *** co-delivering a chemically modified donor DNA with the WDV-Gate plasmid, 3xFLAG peptides were successfully fused to three loci with an efficiency of up to 13%. Thus, by combining transiently expressed sgRNAs and a surrogate selection system, WDV-Gate could be useful for high-throughput gene knock-out and sequence knock-in.

关键词： genome editing high-throughput protein tagging rice

基于国际功能、残疾和健康分类的护理干预在脑卒中吞咽障碍中的应用

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中国实用护理杂志 2017年第31期33卷 2418-2422页

作者：杨峰乔鸿飞李艳雷倩曹雪宁西安交通大学第二附属医院康复医学科 710004

目的观察基于国际功能、残疾和健康分类（ICF）的护理干预措施对脑卒中吞咽障碍患者的影响.方法选择西安交通大学第二附属医院康复医学科脑卒中吞咽障碍患者共60例,按随机数字表法分为对照组和干预组各30例,2组均进行常规的摄食直接... 详细信息

目的观察基于国际功能、残疾和健康分类（ICF）的护理干预措施对脑卒中吞咽障碍患者的影响.方法选择西安交通大学第二附属医院康复医学科脑卒中吞咽障碍患者共60例,按随机数字表法分为对照组和干预组各30例,2组均进行常规的摄食直接训练、口腔护理、薄荷脑冰棒刺激、球囊扩张治疗、吞咽电刺激、吞咽基础训练和健康知识宣教.干预组在常规护理的同时并在董艺提炼的34条吞咽障碍ICF核心项目上给予评估和护理干预,干预前、干预4周和8周时,采用洼田饮水试验进行等级评定,行表面肌电图（sEMG）检查获取咬肌、口轮匝肌、颏下肌群和舌骨下肌群的吞咽时程和最大振幅并进行比较.结果对照组和干预组患者干预前、干预4周、干预8周洼田饮水试验分级分别为（4.12±0.23）、（3.34±0.57）、（2.76±0.46）分和（4.29±0.25）、（2.82±0.39）、（1.81±0.41）分,组内比较差异有统计学意义（F=67.19、368.56,P〈0.01）;干预4周和8周2组比较差异有统计学意义（t=4.81、7.22,P〈0.01）.对照组干预前、干预4周、干预8周咬肌、口轮匝肌、颏下肌群、舌骨下肌群吞咽时程组内比较差异有统计学意义（F=18.40~34.65,均P〈0.01）;干预组干预前、干预4周、干预8周咬肌、口轮匝肌、颏下肌群、舌骨下肌群吞咽时程组内比较差异有统计学意义（F=127.82~174.93,均P〈0.01）;干预4周和8周咬肌、口轮匝肌、颏下肌群、舌骨下肌群吞咽时程2组比较差异有统计学意义（t=-5.32~10.14,均P〈0.01）.对照组咬肌、口轮匝肌、颏下肌群、舌骨下肌群最大振幅干预前、干预4周、干预8周组内比较差异有统计学意义（F=28.11~90.54,均P〈0.01）;干预组咬肌、口轮匝肌、颏下肌群、舌骨下肌群最大振幅干预前、干预4周、干预8周组内比较差异有统计学意义（F=60.91~178.31,均P〈0.01）;干预4周和8周2组比较差异有统计学意义（t=-8.82^-4.06,P〈0.01）.结论基于ICF的护理干预措施对脑卒中吞咽障碍患者有明显的效果.

关键词：国际功能残疾和健康分类护理干预吞咽障碍

天津南港吹填土厚度变化对地表峰值加速度的影响

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工程勘察 2021年第9期49卷 30-33页

作者：徐丹丹李明明徐朦曹雪宁北京市勘察设计研究院有限公司北京100038

场地条件对地震波有明显的放大或缩小作用。工程抗震设防确定工程场地设计地震动参数时,必须考虑工程场地条件的影响。本文选用一处工程实例中的地震危险性计算结果,采用一维等效线性化波动方法,研究了吹填土软弱层厚度对地表峰值加速... 详细信息

场地条件对地震波有明显的放大或缩小作用。工程抗震设防确定工程场地设计地震动参数时,必须考虑工程场地条件的影响。本文选用一处工程实例中的地震危险性计算结果,采用一维等效线性化波动方法,研究了吹填土软弱层厚度对地表峰值加速度的影响。研究结果表明:在不同的地震动输入条件下,输入的地震动强度越小,地表峰值加速度放大效应越大;地表峰值加速度放大倍数随着吹填土软土层厚度的增加先增大后减小,而最大放大倍数所对应的软土层厚度与地震动输入的大小有关。本文所得结果有助于在地震安全性评价工作中把握重点,为制定出合理可靠的工作方案提供依据,从而减少土层地震反应分析过程中的不确定性,提高抗震设防参数的精度。

关键词：抗震设防吹填场地地震动地震反应地表峰值加速度

Controlling the size of fragrance microcapsules using designed agitator paddles: Experiment and CFD simulation

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Particuology 2019年第2期17卷 38-45页

作者： Hongbin Zhao xuening Fei Baolian Zhang Shuxing Zhao Guangmin Li Lingyun cao School of Chemical Engineering and Technology Tianjin University Tianjin 300350 China School of Science Tianjin Chengjian University Tianjin 300384 China School of Materials Science and Engineering Tianjin Chengjian University Tianjin 300384 China School of Energy and Safety Engineering Tianjin Chengjian University Tianjin 300384 China

Controlling the size of fragranee microcapsules using designed agitator paddles was investigated and studied by CFD simulation. First, different fluid flows were established by varying stirring speeds, reactor scales, and agitator paddle design, and the effects of each on particle size and distribution of prepared microcapsules were determined. The experimental results showed that the pattern design of orifices in the plate paddles control the flow field well. Narrow particle-size distributio ns of the microcapsules were obtained. The fluid flow characteristics including fluid velocity field, turbulent kinetic energy field, and shear stress distribution for thedifferent agitator paddle types in different reaction kettles were simulated using CFD tech no logy. The correlations between simulated data and experime ntal results were an alyzed. Significantly, the simulated average flow velocity was found to show good negative linear correlation w让h the average particle size of prepared microcapsules, with a correlation of y =-2.166x + 42.626.

关键词： Fragrance microcapsule Melamine-formaldehyde Particle size Orifice stirring paddle CFD simulation

Characterization and determinant factors of critical illness and in-hospital mortality of COVID-19 patients: A retrospective cohort of 1,792 patients in Kenya

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Biosafety and Health 2022年第5期4卷 330-338页

作者： Isinta M Elijah Endawoke Amsalu xuening Jian Mingyang cao Eric K Mibei Danvas O Kerosi Francis G Mwatsahu Wei Wang Faith Onyangore Youxin Wang Beijing Key Laboratory of Clinical Epidemiology School of Public HealthCapital Medical UniversityBeijing 100069China Armauer Hansen Research Institute Ministry of HealthAddis Ababa 1005Ethiopia University of Kabianga School of Health SciencesKericho 2030-20200Kenya Medical Genetics Laboratory School of Life ScienceCentral South UniversityChangsha 410083China Department of Environmental Health and Disease Control School of Public HealthJomo Kenyatta University of Agriculture and TechnologyNairobi 62000–00200Kenya Centre for Precision Medicine Edith Cowan UniversityPerth WA 6027Australia

Limited data is available on the coronavirus disease 2019(COVID-19),critical illness rate,and in-hospital mortality in the African *** study investigates determinants of critical illness and in-hospital mortality among COVID-19 patients in *** conducted a retrospective cohort study at Kenyatta National Hospital(KNH)in *** logistic regression and Cox proportional hazard regression were employed to determine predictor factors for intensive care unit(ICU)admission and in-hospital mortality,*** addition,the Kaplan-Meier model was used to compare the survival times using log-rank *** a result,346(19.3%)COVID-19 patients were admitted to ICU,and 271(15.1%)*** majority of those admitted to the hospital were male,1,137(63.4%)and asymptomatic,1,357(75.7%).The most prevalent clinical features were shortness of breath,fever,and dry *** addition,older age,male,health status,patient on oxygen(O2),oxygen saturation levels(SPO2),headache,dry cough,comorbidities,obesity,cardiovascular diseases(CVDs),diabetes,chronic lung disease(CLD),and malignancy/cancer can predicate the risk of ICU admission,with an area under the receiver operating characteristic curve(AUC-ROC)of 0.90(95%confidence interval[CI]:0.88–0.92).Survival analysis indicated 271(15.1%)patients died and identified older age,male,headache,shortness of breath,health status,patient on oxygen,SPO2,headache,comorbidity,CVDs,diabetes,CLD,malignancy/cancer,and smoking as risk factors for mortality(AUC-ROC:0.90,95%CI:0.89–0.91).This is the first attempt to explore predictors for ICU admission and hospital mortality among COVID-19 patients in Kenya.

关键词： Comorbidities Critical illness ICU COVID-19 SARS-CoV-2

Fabrication and biological evaluation of 3D-printed calcium phosphate ceramic scaffolds with distinct macroporous geometries through digital light processing technology

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Regenerative Biomaterials 2022年第1期9卷 389-403页

作者： Jing Wang Yitao Tang Quanle cao Yonghao Wu Yitian Wang Bo Yuan Xiangfeng Li Yong Zhou xuening Chen Xiangdong Zhu Chongqi Tu Xingdong Zhang National Engineering Research Center for Biomaterials College of Biomedical EngineeringSichuan UniversityChengdu 610064China Department of Orthopaedics West China Hospital of Sichuan UniversityChengdu 610041China

Digital light processing(DLP)-based 3D printing technique holds promise in fabricating scaffolds with high *** raw calcium phosphate(CaP)powders were modified by 5.5%monoalcohol ethoxylate phosphate(MAEP)to ensure high solid loading and low *** rheological tests found that photocurable slurries composed of 50wt%modified CaP powders and 2wt%toners were suitable for DLP *** on geometric models designed by computer-aided design(CAD)system,three printed CaP ceramics with distinct macroporous structures were prepared,including simple cube,octet-truss and inverse face-centered cube(fcc),which presented the similar phase composition and microstructure,but the different macropore *** fcc group showed the highest porosity and compressive *** in vitro and in vivo biological evaluations were performed to compare the bioactivity of three printed CaP ceramics,and the traditional foamed ceramic was used as *** suggested that all CaP ceramics exhibited good biocompatibility,as evidence by an even bone-like apatite layer formation on the surface,and the good cell proliferation and spreading.A mouse intramuscular implantation model found that all of CaP ceramics could induce ectopic bone formation,and foam group had the strongest osteoinduction,followed by inverse fcc,while cube and octet-truss had the weakest *** indicated that macropore geometry was of great importance to affect the osteoinductivity of scaffolds,and spherical,concave macropores facilitated *** findings provide a strategy to design and fabricate high-performance orthopedic grafts with proper pore geometry and desired biological performance via DLP-based 3D printing technique.

关键词： digital light processing(DLP) 3D printing pore structure osteoinduction

饭圈文化乱象对青少年的影响及加强思想政治教育引导研究

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山东商业职业技术学院学报 2023年第6期23卷 97-100,105页

作者：曹丙燕吕雪宁山东科技大学马克思主义学院山东青岛266590 烟台市水业集团有限公司山东烟台264003

饭圈文化乱象对青少年的负面影响,主要表现为外在物质层面的消费异化和内在精神层面的自我异化。导致异化的原因是青少年因建构个体自我认同感与社会群体归属感的成长需要,逐渐对饭圈文化乱象处于盲目接受状态。针对异化问题,从思想政... 详细信息

饭圈文化乱象对青少年的负面影响,主要表现为外在物质层面的消费异化和内在精神层面的自我异化。导致异化的原因是青少年因建构个体自我认同感与社会群体归属感的成长需要,逐渐对饭圈文化乱象处于盲目接受状态。针对异化问题,从思想政治教育角度重塑青少年的价值观念,主要是通过政治教育厚植家国情怀、法治教育明确是非观念、榜样教育树立理想信念、主流文化突出价值引导、网络引导助推校园教育。

关键词：饭圈文化乱象青少年主体异化思想政治教育

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藜麦皂苷灌胃后大鼠尿液代谢及肠道菌变化

现代食品科技 2022年第8期38卷 1-9页

作者：张若愚闫菲李雪宁曹雨晴余远冯静薛鹏潍坊医学院公共卫生学院山东潍坊261200 潍坊医学院附属医院儿科山东潍坊261200 潍坊医学院临床医学院山东潍坊261200 潍坊医学院康复学院山东潍坊261200

以藜麦皂苷为材料,对成年健康Sprague Dawley大鼠进行90 d经口暴露后结合肠道菌群及尿液代谢组学研究,探究其对肾脏的影响。实验分别将雌雄大鼠(n=48)分为高剂量(500 mg/kg)皂苷灌胃组、中剂量(50 mg/kg)皂苷灌胃组、低剂量(5 mg/kg)皂... 详细信息

以藜麦皂苷为材料,对成年健康Sprague Dawley大鼠进行90 d经口暴露后结合肠道菌群及尿液代谢组学研究,探究其对肾脏的影响。实验分别将雌雄大鼠(n=48)分为高剂量(500 mg/kg)皂苷灌胃组、中剂量(50 mg/kg)皂苷灌胃组、低剂量(5 mg/kg)皂苷灌胃组与空白对照组,经口灌胃处理后,对肠道内容物进行16S rDNA分析,对尿液代谢物采用UPLC-MS分析,探究皂苷对大鼠的肠道菌群及代谢组学的影响。在雌雄大鼠中高剂量组大鼠肾脏切片均发生部分炎性变化。代谢组学结果显示,中低剂量组与空白对照组相比无明显差异,高剂量组大鼠在藜麦皂苷灌胃后,雌性大鼠尿液中发现差异代谢物101种,雄性大鼠尿液中发现差异代谢物29种,影响了维生素B6代谢、氨循环、色氨酸代谢等代谢过程。肠道菌群分析显示,藜麦皂苷灌胃组与对照组大鼠肠道菌群多样性差异明显。综上所述,藜麦皂苷在低剂量下无明显的毒性反应,然而在不可接触的中高剂量下有潜在的肾毒性。

关键词：藜麦皂苷 Sprague Dawley大鼠肠道菌群代谢组学肾脏