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Covalent stabilization of DNA nanostructures on cell membranes for efficient surface receptor-mediated labeling and function regulations

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Science China Chemistry 2022年第11期65卷 2327-2334页

作者： Dandan Chao xuemei xu Yanyan Miao Linlin Yang Qianqian Gao Rui xu Yuan Tian Yumeng Zhao Yuzhen Du Da Han Institute of Molecular Medicine Shanghai Key Laboratory for Nucleic Acid Chemistry and NanomedicineState Key Laboratory of Oncogenes and Related GenesRenji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Department of Laboratory Medicine Shanghai Jiao Tong University Affiliated Sixth People’s HospitalShanghai 200127China

One major challenge of using DNA nanostructures for cellular and in vivo applications is their insufficiently structural integrity that stems from the non-covalent base pairing and stacking in complex cellular and physiological environment. The establishment of covalent bonds in DNA nanostructures can link individual strands more stably and therefore should improve the performance of DNA nanostructures in different scenarios where structural integrity is required. Here, we developed a convenient and effective method for constructing covalently stabilized DNA nanostructures by chemically inserting photo-crosslinker(^(CNV)K) in DNA sequences. These covalently linked DNA nanostructures were found to be more resistant to external interference, such as low cation concentrations and unspecific displacement on cell membranes. We also demonstrated that our strategy could improve the efficiency of cell surface receptor-mediated labeling and function regulations in living cells, which sheds light on broadening the biomedical applications of DNA nanostructures.

关键词： DNA nanostructures covalent stabilization structural integrity cell labeling surface receptor regulation

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罪与罚:清朝废官军台效力赎罪研究

吉林师范大学学报（人文社会科学版） 2024年第3期52卷 12-20页

作者：徐雪梅辽宁大学历史学部辽宁沈阳110031

废官军台效力赎罪是清朝废官制度的重要内容,是针对“犯私罪之人”的惩治性举措。从将废官解送京师、发配军台,到废官服役于军台,直至捐赎与宽免,清朝都定有明章。在这一过程中,不仅体现出清朝中央及地方各部门间的联动关系、职官问责... 详细信息

废官军台效力赎罪是清朝废官制度的重要内容,是针对“犯私罪之人”的惩治性举措。从将废官解送京师、发配军台,到废官服役于军台,直至捐赎与宽免,清朝都定有明章。在这一过程中,不仅体现出清朝中央及地方各部门间的联动关系、职官问责制度对官员的监督与约束,而且也彰显出清帝对用人取舍之权的掌控,皇帝是所有环节的最终裁决者。虽然清帝煞费苦心地区别各类废官,并对各种捐赎情况进行了审慎的分类,以期不同性质的废官都能受到相应的法律制裁和经济惩罚,但在封建王朝周期性政治危机的影响下,军台效力赎罪之制具有很大的局限性,最终在清末沦为一纸空文。

关键词：清朝废官军台效力赎罪

SIRT2 interacts with DDX24 to promote nasopharyngeal carcinoma growth

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BIOCELL 2023年第11期47卷 2445-2452页

作者： HAIYING YUE CHUNHUI WANG HUIJUN ZHU QINGHUA DU JIAN LI xuE OU XIANGDE LI QIULU ZHONG YITING XIE DANJING LUO YIHE LI CHUNXIAO LIANG xuemei xu SONGNAN DU WENQI LIU Department of Radiotherapy The Second Affiliated Hospital of Guangxi Medical UniversityNanningChina

Background:Nasopharyngeal carcinoma(NPC)is one of the most prevalent cancers in Southeast Asia.Sirtuin 2(SIRT2)is a member of the NAD+-dependent deacetylase family and has been shown to play important roles in numerous biological processes.However,Its function in NPC remains uncertain.The primary aim of this study is to clarify the role of SIRT2 in NPC.Methods:In this research,we examined the effect of SIRT2 silencing on NPC cell proliferation and colony formation using vitro NPC cell lines.Co-immunoprecipitation and mass spectrometry was applied to identify SIRT2-interacting proteins in NPC cells.Results:In comparison to nasopharyngeal epithelial NP69 cells,SIRT2 was up-regulated in multiple NPC cell lines,particularly in CNE2 cells.SIRT2 knockdown abrogated CNE2 cell proliferation and colony formation,whereas SIRT2 overexpression promoted HNE1 cell proliferation and colony formation.The SIRT2-interacting proteins were gathered in gene expression and regulation processes including RNA processing and translation.Among the SIRT2-interacting proteins,there were multiple DEAD-box(DDX)family members.Of note,silencing of DDX24 phenocopied the effect of SIRT2 knockdown on NPC growth.Overexpression of DDX24 restored SIRT2-depleted CNE2 cells to proliferative and colony formation.Conclusions:Our study indicates that SIRT2 can interact with DDX24 to enhance NPC growth.The clinical relevance of SIRT2 and DDX24 in NPC warrants further investigation.

关键词： Nasopharyngeal carcinoma Sirtuin 2 DDX24 Proliferation

A homogenous microstructural Mg-based matrix model for orthopedic application with generating uniform and smooth corrosion product layer in Ringer’s solution:Study on biodegradable behavior of Mg-Zn alloys prepared by powder metallurgy as a case

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Journal of Magnesium and Alloys 2021年第1期9卷 225-240页

作者： Yang Yan Xin Chu Xier Luo xuemei xu Yu Zhang YiLong Dai Ding Li Liangjian Chen Tao Xiao Kun Yu School of Materials Science and Engineering Central South UniversityChangsha 410083China Department of Materials Science and Engineering Yantai Nanshan UniversityYantai 265713China The Second Xiangya Hospital Central South UniversityChangsha 410011China The Third Xiangya Hospital Central South UniversityChangsha 410011China

For high corrosion resistance and extensively modified biodegradable Mg-based alloys and composites for bone implants,a new Mgbased matrix model prepared by powder metallurgy is discussed and developed.In this research,Mg-5 wt.%Zn alloys were selected as a case.And they were impacted by hot extrusion and aging treatments to construct microstructure with different characteristics.Their selfforming corrosion product layer in Ringer’s solution,biodegradable behavior and corrosion mechanism were minutely investigated by in vitro degradation,electrochemical corrosion and cytocompatibility.The results demonstrated the extruded Mg-5 wt.%Zn alloy aged for 96 h showed high corrosion resistance,good biocompatibility for L929 and excellent ability of maintaining sample integrity during the immersion.Significantly,the alloy showed fine-grain microstructure and uniform distributed hundred nano-sized second phases,which promoted the formation of the uniform and smooth corrosion product layer at the beginning of immersion.The corrosion product layer was more stable in chloride containing aqueous solution and could be directly formed and repaired quickly,which effectively protected the matrix from further corrosion.In addition,an ideal model of Mg-based matrix for bone tissue engineering was tried to presume and propose by discussing the causal relationship between microstructure and bio-corrosion process.

关键词： Magnesium alloys Powder metallurgy Second phase Corrosion product layer Biodegradable behavior Cytocompatibility

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电工实验台热继电器故障频发的研究与分析

中国现代教育装备 2024年第9期 47-50页

作者：徐航沈烨徐雪梅四川大学电气工程学院四川成都610065

电工实验室核心过载保护装置热继电器的频繁损坏,严重影响实验的顺利进行。随着电工实验台模块的不断增加,电路结构复杂程度不断上升,实验中频繁使用热继电器会导致其损坏越来越多,进而使实验准备时间成倍增加,极大地影响了学生做实验... 详细信息

电工实验室核心过载保护装置热继电器的频繁损坏,严重影响实验的顺利进行。随着电工实验台模块的不断增加,电路结构复杂程度不断上升,实验中频繁使用热继电器会导致其损坏越来越多,进而使实验准备时间成倍增加,极大地影响了学生做实验的积极性。针对以上问题及实验教学的实际需要,采用COMSOL Multiphysics有限元仿真软件,结合Siemens NX软件完成热继电器线圈在持续工作电流及短路电流下,电流、电磁及温度三场的耦合仿真,并通过与实际数据对比分析查找热继电器频繁损坏的根本原因。这为之后的建模仿真打下良好的基础,同时也为实验室继电保护模块的选型提供了依据。

关键词：热继电器 COMSOL Multiphysics 三场耦合继电保护

肠镜下分级对非血缘脐血移植后恶性血液病患者肠道急性移植物抗宿主病的预后评估价值

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中华血液学杂志 2024年第5期45卷 462-467页

作者：汪森林孙光宇朱小玉徐雪梅叶飞李世兰陈思中国科学技术大学附属第一医院(安徽省立医院)消化内科合肥230001 中国科学技术大学附属第一医院(安徽省立医院)血液内科合肥230001 中国科学技术大学附属第一医院(安徽省立医院)临床病理中心合肥230001

目的探讨肠镜下分级对非血缘脐血移植(UCBT)后出现肠道急性移植物抗宿主病(IT-aGVHD)的恶性血液病患者的预后评估价值。方法收集2016年6月至2023年6月在安徽省立医院进行UCBT后出现激素耐药的IT-aGVHD患者50例, 比较肠镜下黏膜损伤较轻... 详细信息

目的探讨肠镜下分级对非血缘脐血移植(UCBT)后出现肠道急性移植物抗宿主病(IT-aGVHD)的恶性血液病患者的预后评估价值。方法收集2016年6月至2023年6月在安徽省立医院进行UCBT后出现激素耐药的IT-aGVHD患者50例, 比较肠镜下黏膜损伤较轻组(27例, 肠镜下分级为Ⅰ、Ⅱ级)和较重组(23例, 肠镜下分级为Ⅲ、Ⅳ级)患者IT-aGVHD治疗的有效率、生存率等, 回顾性分析影响患者预后的因素。结果轻症组、重症组患者在肠镜检查后28 d有效率分别为92.6%和47.8%(P<0.001), 56 d有效率分别为81.5%和39.1%(P=0.002), 最优有效率分别为92.6%和65.2%(P=0.040), 差异均有统计学意义。多因素分析发现, 肠镜下分级是影响IT-aGVHD治疗有效率的独立危险因素。轻症组、重症组患者肠镜检查后2年的总生存率分别为70.4%(95%CI 52.0%~88.8%)和34.8%(95%CI 14.8%~54.8%), 差异有统计学意义(P=0.003)。多因素分析显示, 肠镜下分级、巨细胞病毒感染状态、二线治疗方案及患者的年龄是影响生存的独立危险因素。结论肠镜下黏膜损伤程度较轻组(Ⅰ、Ⅱ级)患者的治疗有效率和预后优于损伤程度较重组(Ⅲ、Ⅳ级)。

关键词：脐血干细胞移植移植物抗宿主病结肠镜检查

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电机学虚拟教学辅助平台的开发与探索

自动化应用 2024年第12期65卷 273-276页

作者：徐航孙曼吴海祥徐雪梅沈烨四川大学电气工程学院四川成都610065 湖南云箭集团有限公司湖南长沙419503

电机学课程实践性很强,在电气工程专业具有广泛应用。针对此课程以抽象理论为主、电机综合实验平台数量有限及实验项目和类型相对固定的特点,基于LabVIEW开发了一套电机学虚拟辅助教学系统。该系统实现了电机多模态控制、动态显示结果... 详细信息

电机学课程实践性很强,在电气工程专业具有广泛应用。针对此课程以抽象理论为主、电机综合实验平台数量有限及实验项目和类型相对固定的特点,基于LabVIEW开发了一套电机学虚拟辅助教学系统。该系统实现了电机多模态控制、动态显示结果及多参数任意可调,可应用在理论教学、实验教学及自动控制类科研项目中。实践表明,这种全新的教学辅助平台可以促进教学观念与教学模式的改革,具有重要的实际意义。

关键词：电机学虚拟辅助教学改革

富血小板血浆治疗膝骨关节炎的临床疗效及对血清胃饥饿素、HMGB1水平的影响

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分子诊断与治疗杂志 2024年第3期16卷 429-432,437页

作者：蔡良罗丹袁琼徐雪梅自贡市第一人民医院输血科四川自贡643000

目的探讨富血小板血浆(PRP)治疗膝骨关节炎的临床疗效及对患者血清胃饥饿素、高迁移率族蛋白1(HMGB1)水平的影响。方法选取2021年1月至2023年1月自贡市第一人民医院收治的膝骨关节炎患者106例为研究对象,根据治疗方法分为PRP治疗组和... 详细信息

目的探讨富血小板血浆(PRP)治疗膝骨关节炎的临床疗效及对患者血清胃饥饿素、高迁移率族蛋白1(HMGB1)水平的影响。方法选取2021年1月至2023年1月自贡市第一人民医院收治的膝骨关节炎患者106例为研究对象,根据治疗方法分为PRP治疗组和透明质酸治疗组,各53例。比较两组治疗效果及血清胃饥饿素、HMGB1水平。结果 PRP组治疗后1、3个月膝关节损伤及骨关节炎调查问卷(KOOS)评分、Lysholm评分、骨密度高于透明质酸组,差异有统计学意义(t=3.235、10.437、5.494、3.863、4.183、10.268,P0.05)。结论 PRP可以提高血清胃饥饿素水平,降低HMGB1水平,改善膝骨关节炎患者关节功能,缓解疼痛提高生活质量。

关键词：富血小板血浆膝骨关节炎胃饥饿素高迁移率族蛋白1

Strategies for the development and approval of COVID-19 vaccines and therapeutics in the post-pandemic period

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Signal Transduction and Targeted Therapy 2024年第1期9卷 19-35页

作者： Danyi Ao xuemei He Jian Liu Li xu State Key Laboratory of Biotherapy West China HospitalSichuan University610041 SichuanPeople’s Republic of China

The spread of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has resulted in significant casualties and put immense strain on public health systems worldwide,leading to economic recession and social unrest.In response,various prevention and control strategies have been implemented globally,including vaccine and drug development and the promotion of preventive measures.Implementing these strategies has effectively curbed the transmission of the virus,reduced infection rates,and gradually restored normal social and economic activities.However,the mutations of SARS-CoV-2 have led to inevitable infections and reinfections,and the number of deaths continues to rise.Therefore,there is still a need to improve existing prevention and control strategies,mainly focusing on developing novel vaccines and drugs,expediting medical authorization processes,and keeping epidemic surveillance.These measures are crucial to combat the Coronavirus disease(COVID-19)pandemic and achieve sustained,long-term prevention,management,and disease control.Here,we summarized the characteristics of existing COVID-19 vaccines and drugs and suggested potential future directions for their development.Furthermore,we discussed the COVID-19-related policies implemented over the past years and presented some strategies for the future.

关键词： prevention sustained approval