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Application of surface-enhanced laser desorption/ionization time-of-flight-based serum proteomic array technique for the early diagnosis of prostate cancer

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Asian Journal of Andrology 2006年第1期8卷 45-51页

作者： Yu-Zhuo Pan xue-yuan xiao Dan Zhao Guo-Yi Ji Yang Li Bao-xue Yang Da-Cheng He xue-Jian Zhao Research Center of Prostate Diseases Department of Reproductive Pathophysiology School of Basic Medicine JilinUniversity Changchun 130021 China Research Institute of Cytobiology of the Academy of Life Science Research Institute of Proteomics Normal UniversityBeijing 100875 China

Aim： To identify the serum biomarkers of prostate cancer （PCa） by protein chip and bioinformatics. Methods： Serum samples from 83 PCa patients and 95 healthy men were taken from a mass screening in Changchun, China. Protein profiling was carried out using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry （SELDI-TOF MS）. The data of spectra were analyzed using two bioinformatics tools. Results： Eighteen serum differential proteins were identified in the PCa group compared with the control group （P 〈 0.01）. There were four proteins at the higher serum level and 14 proteins at the lower serum level in the PCa group. A decision tree classification algorithm that used an eight-protein mass pattern was developed to correctly classify the samples. A sensitivity of 92.0 % and a specificity of 96.7 % for the study group were obtained by comparing the PCa and control groups. Conclusion： We identified new serum biomarkers of PCa. SELDI-TOF MS coupled with a decision tree classification algorithm will provide a highly accurate and innovative approach for the early diagnosis of PCa. （Asian J Androl 2006 Jan; 8： 45-51）

关键词： prostate cancer early diagnosis protein chip biomarker serum

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现代武术与传统文化的崩离与融合

成都体育学院学报 2008年第7期34卷 50-53页

作者：薛晓媛山西财经大学体育系山西太原030006

中国武术的现代性问题一直是人们争论和关注的武术发展问题,更是中国武术在当代遭遇发展瓶颈亟待深入思考和反省的问题。运用文献资料研究方法,从"传统"与"现代"的文化悖论着眼,分析了中国武术在现代社会中现代性与传统崩离的客观现实... 详细信息

中国武术的现代性问题一直是人们争论和关注的武术发展问题,更是中国武术在当代遭遇发展瓶颈亟待深入思考和反省的问题。运用文献资料研究方法,从"传统"与"现代"的文化悖论着眼,分析了中国武术在现代社会中现代性与传统崩离的客观现实及历史、文化成因,并进一步论述了应如何将中国武术现代性与传统融合,从而走出发展的瓶颈。

关键词：中国武术现代性传统崩离融合

Protein Profile of Human Lung Squamous Carcinoma Cell Line NCI-H226

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Biomedical and Environmental Sciences 2007年第1期20卷 24-32页

作者： HAO ZHANG NA LI YUE CHEN LING-YUN HUANG YI-CHING WANG GANG FANG DA-CHENG HE xue-yuan xiao Key Laboratory of Cell Proliferation and Regulation of Ministry of Education Universities＇ Confederated Institute of Proteomics Beijing Normal University Beijing 100875 China Department of Life Sciences National Taiwan Normal Universities Sec. 4 Tingchou Rd Taipei 116 Taiwan

Objective To construct a database of human lung squamous carcinoma cell line NCI-H226 and to facilitate discovery of novel subtypes markers of lung cancer. Method Proteomic technique was used to analyze human lung squamous carcinoma cell line NCI-H226. The proteins of the NCI-H226 cells were separated by two-dimensional gel electrophoresis and identified by mass spectrometry. Results The results showed that a good reproducibility of the 2-D gel pattern was attained. The position deviation of matched spots among three 2-D gels was 1.95±0.53 mm in the isoelectric focusing direction, and 1.73±0.45 mm in the sodium dodecyl sulfate-polyacrylamide gel electrophoresis direction. One hundred and twenty-seven proteins, including enzymes, signal transduction proteins, structure proteins, transport proteins, etc. were characterized, of which, 29 identified proteins in NCI-H226 cells were reported for the first time to be involved in lung cancer carcinogenesis. Conclusion The information obtained from this study could provide some valuable clues for further study on the carcinogenetic mechanism of different types of lung cancer, and may help us to discover some potential subtype-specific biomarkers of lung cancer.

关键词： Lung squamous carcinoma NCI-H226 cell line Proteomics Two-dimensional datapase

Observation of acoustic hybrid topological phases induced by the p-d orbital interactions

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 132-139页

作者： xiao Xiang Feng Gao Yu-Gui Peng Peng Wu Zong-Lin Li xue-Feng Zhu School of Physics and Innovation Institute Huazhong University of Science and Technology

In spinless systems,growing attention has recently been attracted to synthetic gauge fields,which redefine the fundamental crystal symmetries by utilizing the projective algebraic ***,synthetic gauge fields have been commonly investigated in single orbital systems,and explorations on hybrid orbitals remain elusive in ***,we report the experimental observation of hybrid topological phases induced by hybrid synthetic gauge flux,which is formed by the interaction between p and d orbitals embedded in acoustic *** breaking the translation symmetries of Lxand/or Ly,we unambiguously demonstrate the first-order M?bius edge states and higher-order corner *** work establishes a platform for seeking exotic topological phases induced by hybrid-orbitals interactions and initializing the framework of hybrid-orbitalsrelated topological *** applications can be anticipated in scenarios such as high-quality sensing and robust wave trapping due to the stepwise energy distribution of the hybrid topological phase.

关键词： topological insulator synthetic gauge field projective symmetry corner states

Spontaneous Orientation Polarization of Anisotropic Equivalent Dipoles Harnessed by Entropy Engineering for Ultra‑Thin Electromagnetic Wave Absorber

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Nano-Micro Letters 2025年第1期17卷 424-438页

作者： Honghan Wang Xinyu xiao Shangru Zhai Chuang xue Guangping Zheng Deqing Zhang Renchao Che Junye Cheng Liaoning Key Lab of Lignocellulose Chemistry and BioMaterials Liaoning Collaborative Innovation Center for Lignocellulosic BiorefinerySchool of Light Industry and Chemical EngineeringDalian Polytechnic UniversityDalian 116034People’s Republic of China School of Life Science and Biotechnology Dalian University of TechnologyDalian 116024People’s Republic of China Department of Mechanical Engineering Hong Kong Polytechnic UniversityHung HomKowloonHong Kong 999077People’s Republic of China School of Materials Science and Engineering Qiqihar UniversityQiqihar 161006People’s Republic of China Laboratory of Advanced Materials Shanghai Key Lab of Molecular Catalysis and Innovative MaterialsAcademy for Engineering&TechnologyFudan UniversityShanghai 200438People’s Republic of China Department of Materials Science Shenzhen MSU-BIT UniversityShenzhen 517182People’s Republic of China

The synthesis of carbon supporter/nanoscale high-entropy alloys(HEAs)electromagnetic response composites by carbothermal shock method has been identified as an advanced strategy for the collaborative competition engineering of conductive/dielectric *** migration modes within HEAs as manipulated by the electronegativity,valence electron configurations and molar proportions of constituent elements determine the steady state and efficiency of equivalent ***,enlightened by skin-like effect,a reformative carbothermal shock method using carbonized cellulose paper(CCP)as carbon supporter is used to preserve the oxygencontaining functional groups(O·)of carbonized cellulose fibers(CCF).Nucleation of HEAs and construction of emblematic shell-core CCF/HEAs heterointerfaces are inextricably linked to carbon metabolism induced by O·.Meanwhile,the electron migration mode of switchable electronrich sites promotes the orientation polarization of anisotropic equivalent *** virtue of the reinforcement strategy,CCP/HEAs composite prepared by 35%molar ratio of Mn element(CCP/HEAs-Mn_(2.15))achieves efficient electromagnetic wave(EMW)absorption of−51.35 dB at an ultra-thin thickness of 1.03 *** mechanisms of the resulting dielectric properties of HEAs-based EMW absorbing materials are elucidated by combining theoretical calculations with experimental characterizations,which provide theoretical bases and feasible strategies for the simulation and practical application of electromagnetic functional devices(e.g.,ultra-wideband bandpass filter).

关键词： High-entropy alloys Carbothermal shock Switchable electron migration modes Emblematic shell-core heterointerfaces Ultra-thin thickness

Serum Amyloid A Protein: A Potential Biomarker Correlated With Clinical Stage of Lung Cancer

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Biomedical and Environmental Sciences 2007年第1期20卷 33-40页

作者： DAN-HUI LIU xiao-MIN WANG LI-JUAN ZHANG SONG-WEI DAI LI-YUN LIU JI-FU LIU SHAN-SHAN WU SHUAN-YING YANG SAM FU xue-yuan xiao DA-CHENG HE Key Laboratory of Cell Proliferation and Regulation of Ministry of Education Beijing Normal University Beijing 100875 China Department of Thoracic Surgery General Hospital of Beijing Command PLA Beijing 100700 China Department of Respiratory Medicine Second Hospital of Xi＇an Jiaotong University Xi＇an 710004 Shaanxi China Ciphergen Biosystems Inc. Fremont CA 94555 USA

Objective To identify serum diagnosis or progression biomarkers in patients with lung cancer using protein chip profiling analysis. Method Profiling analysis was performed on 450 sera collected from 213 patients with lung cancer, 19 with pneumonia, 16 with pulmonary tuberculosis, 65 with laryngeal carcinoma, 55 with laryngopharyngeal carcinoma patients, and 82 normal individuals. A new strategy was developed to identify the biomarkers on chip by trypsin pre-digestion. Results Profiling analysis demonstrated that an 11.6kDa protein was significandy elevated in lung cancer patients, compared with the control groups （P〈0.001）. The level and percentage of 11.6kDa protein progressively increased with the clinical stages Ⅰ-Ⅳ and were also higher in patients with squamous cell carcinoma than in other subtypes. This biomarker could be decreased after operation or chemotherapy. On the other hand, 11.6kDa protein was also increased in 50% benign diseases of lung and 13% of other cancer controls. After trypsin pre-digestion, a set of new peptide biomarkers was noticed to appear only in the samples containing a 11.6kDa peak. Further identification showed that 2177Da was a fragment of serum amyloid A （SAA, MW 11.6kDa）. Two of the new peaks, 1550Da and 1611Da, were defined from the same protein by database searching. This result was further confirmed by partial purification of 11.6kDa protein and MS analysis. Conclusion SAA is a useful biomarker to monitor the progression of lung cancer and can directly identify some biomarkers on chip.

关键词： Lung cancer Serum amyloid A On chip identification Surface enhanced laser desorption/ionization Biomarker

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基于水化学特征分析的象山矿井突水水源判别

西安科技大学学报 2017年第2期37卷 213-218页

作者：代革联薛小渊牛超西安科技大学地质与环境学院陕西西安710054 国土资源部煤炭资源勘查与综合利用重点实验室陕西西安710054

为能够快速的、准确的识别矿井突水水源,减少煤矿人员伤亡和经济损失,以象山煤矿为例,应用Piper三线图法对井田内3组基岩含水层(奥陶系灰岩含水层、石炭系砂岩/灰岩含水层、二叠系砂岩裂隙含水层)中水质类型进行划分,采用水质分析对比... 详细信息

为能够快速的、准确的识别矿井突水水源,减少煤矿人员伤亡和经济损失,以象山煤矿为例,应用Piper三线图法对井田内3组基岩含水层(奥陶系灰岩含水层、石炭系砂岩/灰岩含水层、二叠系砂岩裂隙含水层)中水质类型进行划分,采用水质分析对比和系统聚类分析相结合的方法,通过对井田内3组基岩含水层中28个水样进行的分析,获得了不同基岩含水层水的化学特征,根据水化学特征,将突水点处的待定水样和3组基岩含水层中的水样对比来判别矿井突水水源,同时提出了利用模糊聚类方法进一步确定矿井突水水源。研究结果表明,石炭系砂岩(灰岩)水和奥陶系岩溶水的水化学特征都与待定水样比较相似,不能轻易识别;聚类分析法能够排除干扰因素并最终精确判别矿井突水水源,即象山煤矿280排矸石门突水的水源为奥灰岩溶水,预测结果与实际相符,表明该方法针对矿井突水的水源判别具有较好的准确性。

关键词：矿井突水水质分析 Piper三线图聚类分析

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基于放水试验的烧变岩含水层可疏性分析

能源技术与管理 2022年第2期47卷 1-3页

作者：薛小渊中煤科工集团西安研究院有限公司陕西西安710054

以榆神矿区某矿火烧区放水试验为例,根据放水量、井上下水位变化幅度及水质变化情况等资料,结合烧变岩岩性、抽水试验、补径排条件等,综合分析了烧变岩含水层的可疏性。实践表明,放水试验可弥补地面抽水试验的局限性,对于查明煤矿区水... 详细信息

以榆神矿区某矿火烧区放水试验为例,根据放水量、井上下水位变化幅度及水质变化情况等资料,结合烧变岩岩性、抽水试验、补径排条件等,综合分析了烧变岩含水层的可疏性。实践表明,放水试验可弥补地面抽水试验的局限性,对于查明煤矿区水文地质条件有着非常重要的意义,其结果可为矿井防治水提供合理的依据。

关键词：榆神矿区烧变岩放水试验可疏性

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数字全球化、数字风险与全球数字治理

东北亚论坛 2022年第3期31卷 3-18,127页

作者：薛晓源刘兴华北京师范大学全球化与文化发展战略研究院北京519087 南开大学周恩来政府管理学院天津300350

随着数字技术的快速发展,数字全球化进程不断加速,全球数字风险和危机日益凸显,全球数字治理成为国际社会的紧迫议题。厘清数字全球化的概念和特征有助于更好地把握当今全球化转型与发展的大势。数字全球化既为世界发展带来机遇,也容易... 详细信息

随着数字技术的快速发展,数字全球化进程不断加速,全球数字风险和危机日益凸显,全球数字治理成为国际社会的紧迫议题。厘清数字全球化的概念和特征有助于更好地把握当今全球化转型与发展的大势。数字全球化既为世界发展带来机遇,也容易诱发数字鸿沟、数字博弈、数字霸权、数字冷战、数字安全、数字垄断、数字极化、数字政治化、数字自由、数字隐私权等诸多数字风险。为有效应对这些风险,全球数字治理应高度关注数字经济、数字政府、数字伦理、数字舆论、跨境数据流动、数字规则等重要议题,筹谋制定好全球数字治理战略和政策,努力实现数字世界的公平性、非歧视性、非霸权性、开放性、高效性,加强全球数字治理的问题意识,树立全球意识、时间意识、总体意识、创新意识、前瞻意识,在维护国家数字利益的同时促进全球数字福祉。

关键词：数字全球化数字风险全球数字治理议题目标问题意识