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Plant Communications 2024年第3期5卷 179-196页

作者： Beifang Wang pao xue Yingxin Zhang Xiaodeng Zhan Weixun Wu Ping Yu Daibo Chen Junlin Fu Yongbo Hong Xihong Shen Lianping Sun Shihua Cheng Qunen Liu Liyong Cao State Key Laboratory of Rice Biology and Breeding China National Rice Research InstituteHangzhou 311400China Northern Rice Research Center of Bao Qing Shuangyashan 155600China Zhejiang Key Laboratory of Super Rice Research China National Rice Research InstituteHangzhou 311400China

Calcium-dependent protein kinases(CPKs),the best-characterized calcium sensors in plants,regulate many aspects of plant growth and development as well as plant adaptation to biotic and abiotic ***,how CPKs regulate the antioxidant defense system remains largely *** previously found that impaired function of OsCPK12 leads to oxidative stress in rice,with more H2O2,lower catalase(CAT)activity,and lower ***,we explored the roles of OsCPK12 in oxidative stress tolerance in *** results show that OsCPK12 interacts with and phosphorylates OsCATA and OsCATC at *** of either OsCATA or OsCATC leads to an oxidative stress phenotype accompanied by higher accumulation of *** of the phosphomimetic proteins OsCATAS11D and OsCATCS11D in oscpk12-cr reduced the level of H2O2 ***,OsCATAS11D and OsCATCS11D showed enhanced catalase activity in vivo and in ***12-overexpressing plants exhibited higher CAT activity as well as higher tolerance to oxidative *** demonstrate that OsCPK12 affects CAT enzyme activity by phosphorylating OsCATA and OsCATC at Ser11 to regulate H2O2 homeostasis,thereby mediating oxidative stress tolerance in rice.

关键词： OsCPK12 OsCATs oxidative tolerance Oryza sativa L

Effects of GS3 and GL3.1 for Grain Size Editing by CRISPR/Cas9 in Rice

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Rice science 2020年第5期27卷 405-413,I0024页

作者： Chen Yuyu Zhu Aike xue pao Wen Xiaoxia Cao Yongrun Wang Beifang Zhang Yue Shah Liaqat Cheng Shihua Cao Liyong Zhang Yingxin State Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement China National Rice Research InstituteHangzhou 311401China National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China Nanch ong Academy of Agricultural Sciences Ncmchong 637000China

Grain size is one of key agronomic traits associated with grain yield and grain quality. Both major quantitative trait loci GS3 and GL3.1 play a predominant role in negative regulation of grain size. In this study, a CRISPR/Cas9-mediated multiplex genome editing system was used to simultaneously edit GS3 and GL3.1 in a typical japonica rice Nipponbare. In T1 generation, we found that gs3 formed slender grain with lower chalkiness percentage, while gs3gl3.1 produced larger grain with higher chalkiness percentage. In terms of other agronomic traits, flag leaf size, grain number and grain yield of both gs3 and gs3gl3.1 mutants were affected. It is noteworthy that gs3 and gs3gl3.1 mutants both led to dramatical reduction of grain number, thereby decreased grain yield. In conclusion, these results indicated that knockout of GS3 and GL3.1 could rapidly improve grain size, but probably have some negative influences on grain quality and grain yield.

关键词： rice GS3 GL3.1 grain size grain quality grain yield CRISPR/Cas9

QTL Mapping for Plant Height Using Introgression Lines Derived from Zhonghui 8015 and Wild Rice(Oryza rufipogon)

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Rice science 2022年第6期29卷 503-506,I0002-I0006页

作者： YANG Qinqin ZHANG Yingxin xue pao WEN Xiaoxia LIU Ling XU Peng ZHAN Xiaodeng CAO Liyong CHENG Shihua WU Weixun China National Center for Rice Improvement/State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 310006China Northern Center of China National Rice Research Institute Shuangyashan 155600China

Many excellent genes in wild rice have been lost during the domestication of wild rice to cultivated *** this study,introgression lines(ILs)were produced with a wild rice(Oryza rufipogon)accession,BJ194,as a donor parent and an indica restorer line,Zhonghui 8015(ZH8015),as a recipient parent to map QTLs for plant *** identified four QTLs(qPH3.1,qPH3.2,qPH2,and qPH8)related to plant height distributed on chromosomes 2,3 and ***,we sequenced and analyzed qPH3.2 located in the interval of RM15753-RM3525,and found this QTL may be a new locus regulating rice plant height.

关键词： Oryza cultivated Zh

Development of Chromosome Segment Substitution Lines and Genetic Dissection of Grain Size Related Locus in Rice

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Rice science 2021年第4期28卷 322-324,I0012-I0017页

作者： Aamir Riaz Wang Huimin Zhang Zhenhua Workie Anley Zegeye Li Yanhui Wang Hong xue pao Peng Zequn Shen Xi hong Cheng Shihua Zhang Yingxin State Key Laboratory of Rice Biology/National Center for Rice Improvement China National Rice Research InstituteHangzhou 310006China Department of Plant Science University of GondarGondar 196Ethiopia

To investigate the genetic bases of grain weight(GW),a set of 76 chromosome segment substitution lines(CSSLs,BC4F5)were developed from the cross between Xieqingzao B(XQZB)and Zhonghui 9308(ZH9308)using a marker-assisted selection(MAS).All substituted segments represented by 120 markers in those lines covered 96.7%of the donor parent(ZH9308).Consequently,two QTLs(qTGW1 and qTGW6)for 1000-grain weight(TGW)were preliminarily mapped on chromosomes 1 and 6,*** line CSSL7 was selected for further mapping of qGL1.4/*** a result,qGL1.4 was validated and narrowed to a 1.4 Mb interval between markers InDel15 and RM11872 using secondary F2,and narrowed to a 500 kb in F2:3 populations between markers RM11824 and *** F4:5 secondary population,using homozygous recombinant strategy,qGL1.4 was finally fine-mapped to a 286.4 kb region between markers D-12 and *** addition,a near-isogenic line(NIL)harbouring qGL1.4 was developed using MAS approach,which showed enhanced grain length compared with ZH9308 without changing other *** summary,these results lay a foundation for the genetic isolation of qGL1.4 and molecular breeding in rice.

关键词： breeding narrow bases

Mapping and genetic validation of a grain size QTL qGS7.1 in rice(Oryza sativa L.)

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Journal of Integrative Agriculture 2019年第8期18卷 1838-1850页

作者： xue pao ZHANG Ying-xin LOU Xiang-yang ZHU Ai-ke CHEN Yu-yu SUN Bin YU Ping CHENG Shi-hua CAO Li-yong ZHAN Xiao-deng Zhejiang Key Laboratory of Super Rice Research/State Key Laboratory of Rice Biology/China National Rice Research Institute Hangzhou 310006 P.R.China Nanchong Academy of Agricultural Sciences Nanchong 637000 P.R. China National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 P.R.China Institute of Crop Breeding and Cultivation Shanghai Academy of Agricultural Sciences Shanghai 201403 P.R.China

Grain size is a major determinant of grain weight, which is one of the components of rice yield. The objective o this study was to identify novel, and important quantitative trait loci(QTLs) for grain size and weight in rice. QTLs were mapped using a BC4F4 population including 192 backcross inbred lines(BILs) derived from a backcross between Xiaolijing(XLJ) and recombinant inbred lines(RILs). The mapping population was planted in both Lingshui(Hainan, 2015) and Fuyang(Zhejiang, 2016), with the short-and long-day conditions, respectively. A total of 10 QTLs for grain length, four for grain width, four for the ratio of grain length to width, and 11 for grain weight were detected in at least one environment and were distributed across 11 chromosomes. The phenotypic variance explained ranged from 6.76–25.68%, 14.30–34.03%, 5.28–26.50%, and 3.01–22.87% for grain length, grain width, the ratio of grain length to width, and thousand grain weight, respectively. Using the sequential residual heterozygotes(SeqRHs) method, qGS7.1, a QTL for grain size and weight, was mapped in a 3.2-Mb interval on chromosome 7. No QTLs about grain size and weight were reported in previous studies in this region, providing a good candidate for functional analysis and breeding utilization.

关键词： rice (Oryza sativa L.) quantitative trait loci grain size grain weight residual heterozygote

不同剂量丙线照射后小鼠小肠组织乙酰胆碱和胆碱酯酶的变化

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军事医学 1982年第6期33卷 673-679页

作者：薛宝刚张云祥军事医学科学院放射医学研究所

小肠是辐射敏感器官之一,急性辐射损伤时小肠运动功能出现明显紊乱,其变化程度与照射剂量有关。射线引起的小肠运动功能障碍前人已有报导。近年来不少学者提出急性放射病时由于肠套叠而引起的死亡是个值得重视的问题。不难看出,急性放... 详细信息

小肠是辐射敏感器官之一,急性辐射损伤时小肠运动功能出现明显紊乱,其变化程度与照射剂量有关。射线引起的小肠运动功能障碍前人已有报导。近年来不少学者提出急性放射病时由于肠套叠而引起的死亡是个值得重视的问题。不难看出,急性放射病时小肠运动功能紊乱对病程的发展和结局具有一定的影响。关于照射后小肠运动功能紊乱的机理,国内外学者曾做过一些研究。他们认为。

关键词：小肠组织小鼠小家鼠胆碱三甲基烷基铵不同剂量

水稻特大粒种质BG1和优质恢复系华占的粒形基因研究及相关功能标记开发

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中国水稻科学 2021年第6期35卷 543-553页

作者：龚柯薛炮温小霞廖飞飞孙滨彭泽群程式华曹立勇张迎信吴玮勋孙廉平占小登中国水稻研究所/水稻生物学国家重点实验室/浙江省超级稻研究重点实验室杭州311401 上海市农业科学院作物育种栽培研究所上海201403

【目的】特异种质资源的发掘与利用是水稻优质高产育种的重要手段之一。明确特大籽粒种质BG1(Big Grain1)和育种上广泛配组的优质恢复系华占携带的部分粒形基因等位变异类型,并开发相应基因的功能标记,有助于加快粒形基因的育种应用,提... 详细信息

【目的】特异种质资源的发掘与利用是水稻优质高产育种的重要手段之一。明确特大籽粒种质BG1(Big Grain1)和育种上广泛配组的优质恢复系华占携带的部分粒形基因等位变异类型,并开发相应基因的功能标记,有助于加快粒形基因的育种应用,提高水稻粒形精准改良的效率。【方法】对BG1和华占中的9个主效粒形基因(GS3、qLGY3、q GL3、GW2、GW8、GW5、TGW3、TGW6、GS9)的目的片段进行测序分析。并根据GW2、GS3、q GL3、TGW3、TGW6、q LGY3、GW8和GW5的测序结果,开发了鉴定其基因分型的功能标记。【结果】与日本晴相比,特大粒种质BG1在检测的9个主效粒形基因中有5个表现为功能缺失型(gs3、GW8、gw2、tgw3、tgw6),1个稀有等位变异(qgl3)和1个外显子可变剪切型(qlgy3);与日本晴相比,华占在检测的9个主效粒形基因中有3个(gs3、GW8、GW5)存在差异。根据测序结果开发了GW2、GS3、q GL3、TGW3、TGW6、qLGY3、GW8和GW5的功能标记,并利用15个水稻种质进行检测,检测结果中除了TGW6的标记TGW6-Del仅可用于鉴定是否含有功能缺失型的等位变异tgw6外,另外7个标记均为可准确鉴定基因型。【结论】与日本晴相比,特大粒种质BG1中调控粒长的基因gs3、qgl3、qlgy3、tgw3、GW8和tgw6之间可能存在复杂的互作调控通路,而不是简单的效应累加,粒宽特征可能是gw2、GW8的累加效应。优质恢复系华占的细长粒形可能是gs3、GW5和GW8的互作效应所致。本研究中开发的功能标记可以用于水稻分子标记辅助育种。

关键词：粒形粒长粒宽粒重功能标记

Chromosome-level genome assembly of the freshwater snail Bellamya purificata(Caenogastropoda)

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Zoological Research 2022年第4期43卷 683-686页

作者： Wu Jin Xiao-Juan Cao xue-Yan Ma Guo-Hua Lv Gang-Chun Xu pao Xu Bing Sun Dong-Po Xu Hai-Bo Wen Key Laboratory of Integrated Rice-Fish Farming Ecology Ministry of Agriculture and Rural AffairsFreshwater Fisheries Research CenterChinese Academy of Fishery SciencesWuxiJiangsu 214081China Wuxi Fisheries College Nanjing Agricultural UniversityWuxiJiangsu 214128China College of Fisheries Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic BeltMinistry of EducationHuazhong Agricultural UniversityWuhanHubei 430070China

DEAR EDITOR,Bellamya purificata(Gastropoda:Caenogastropoda;Architaenioglossa;Viviparidae:Bellamyinae;Sinotaia),a homotypic synonym of ***,is widely distributed in freshwater habitats in *** is an economically important edible snail and plays a vital function in freshwater wetland ***,genomic resources for this snail are lacking and no reference genome has been *** this study,we constructed the first chromosome-level genome of *** using PacBio long-read sequencing and high-throughput chromosome conformation capture(Hi-C)technology.

关键词： freshwater snail habitat

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不同氮肥利用效率粳稻品种筛选及基因型鉴定

江西农业学报 2023年第9期35卷 29-34页

作者：何弯弯王友霜赵轶鹏薛炮王健康丁成伟丁震乾陈秋丽胡婷婷江苏徐淮地区徐州农业科学研究所江苏徐州221121

以适宜黄淮地区种植的19个常规中熟中粳品种(系)为研究对象,通过设置3个氮肥水平(0、200和300 kg/hm2)研究了不同品种间的氮肥利用效率及农艺性状的变化规律,以此筛选氮高效利用的常规粳稻品种。结果表明:在3种氮肥水平下,19个品种间的... 详细信息

以适宜黄淮地区种植的19个常规中熟中粳品种(系)为研究对象,通过设置3个氮肥水平(0、200和300 kg/hm2)研究了不同品种间的氮肥利用效率及农艺性状的变化规律,以此筛选氮高效利用的常规粳稻品种。结果表明:在3种氮肥水平下,19个品种间的氮肥农学利用率及产量相关因素变异显著,其中氮肥农学利用率与株高、穗长、有效穗数、单株谷重呈极显著正相关,株高、结实率、有效穗数、单株谷重随着氮素含量的增加而提高,每穗粒数、氮肥农学利用率随着施氮量的增加而下降。通过不同氮素水平下的产量相关性状分析,筛选出徐63826和南粳5718等2个氮高效利用品种。利用氮高效利用基因NRT1.1B的分子标记对19份常规中熟中粳品种(系)进行检测,结果表明:徐稻3号、徐稻9号及徐香粳16号等18个品种携带nrt1.1b基因型,仅徐63826携带氮高效利用基因NRT1.1B基因型。结合3种氮素水平下的农艺性状与基因型分析,最终筛选出2个氮高效利用品种,其中徐63826携带NRT1.1B基因,南粳5718携带nrt1.1b基因。

关键词：水稻氮肥利用效率氮高效利用基因

议题设置与国族共同体构建:淞沪会战时期《大公报·临时晚刊》的难民报道研究

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重庆师范大学学报（社会科学版） 2024年第2期44卷 97-106页

作者：薛平龙其林华南农业大学珠江学院广东广州510900 上海交通大学人文学院上海200240

为服务淞沪会战而发行的《大公报·临时晚刊》办报时间虽不长,却具有报道视角集中、版面少、售价低的特点。与正刊相比,《临时晚刊》通过差异化的议题设置,聚焦难民救济政策、生活状况、群体形象等,较早回应了由“逃难”向“抗难”的呼... 详细信息

为服务淞沪会战而发行的《大公报·临时晚刊》办报时间虽不长,却具有报道视角集中、版面少、售价低的特点。与正刊相比,《临时晚刊》通过差异化的议题设置,聚焦难民救济政策、生活状况、群体形象等,较早回应了由“逃难”向“抗难”的呼吁,积极面对难民大量集中所导致的社会和国族伦理失序问题,通过连续性、密集性的难民报道引导教育底层难民,促进了普通民众对民族抗战的认识,推动了现代意义上的国族共同体意识形成,运用兼具新闻性与文学性的纪实类文章使得难民群体成为一类特殊的符号,以难民自身的言说提供了战争环境中个人与国家关系的报刊叙事维度,对研究战争时期临时报刊的议题特点有着重要的启示。

关键词：淞沪会战《大公报·临时晚刊》难民报道议题设置共同体意识