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On Homomorphism of Valuation Algebras

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Communications in Mathematical Research 2011年第1期27卷 69-80页

作者： GUAN xue-chong LI YONG-MING College of Mathematics and Information Science Shaanxi Normal University Xi ＇an 710062 School of Mathematical Science Xuzhou Normal University Xuzhou Jiangsu 221116 College of Computer Science Shaanxi Normal University Xi＇an 710062

In this paper, firstly, a necessary condition and a sufficient condition for an isomorphism between two semiring-induced valuation algebras to exist are presented respectively. Then a general valuation homomorphism ba... 详细信息

关键词： homomorphism valuation algebra semiring

Cloning and expressing a recombinant human tissue inhibitor of metalloproteinase-2(TIMP-2)in methylotrophic yeast Pichia pastoris and its characterizations

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Frontiers in Biology 2007年第2期2卷 170-175页

作者： YAN Xunyou ZHAO Hongliang ZHANG Weiguang xue chong LIU Zhimin Insitute of Biotechnology Academy of Military Medical SciencesBeijing 100071China Biology Department Langfang Normal CollegeLangfang 065000China College of Food Science Southwest Agricultural UniversityChongqing 400716China

To obtain human tissue inhibitor of metalloproteinase-2(TIMP-2)cDNA and the secretory expression of TIMP-2 gene in Pichia pastoris,we designed and synthesized a 618 base pairs artificial gene coding for the TIMP-2 with a computer-aided design method using a standard chemical synthesis technique,which was composed of frequently used codons in the highly expressed Pichia pastoris *** the synthetic gene encoding TIMP-2 was checked by means of dideoxynucleotide *** verified gene of TIMP2 was cloned to the Escherichia coli-yeast shuttle vector of pPIC9 to construct a recombinant plasmid *** plasmid was transformed into GS115 cells of the methylotrophic yeast,Pichia pastoris by electroporation,and we got the expression cell through phenotype selection and induction with ***,purification,and bioactivity analysis of the expressed products were performed.

关键词： tissue inhibitor of metalloproteinase-2 Pichia pastoris expression bioactivity

SYMBOL AND STRUCTURE OF "SONG OF MYSELF"

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外国语文 1982年第3期 48-53页

作者： xue chong

本文是我院77级毕业生薛重在昆明师范学院英美文学专业硕士研究生班学习期间所写的一篇关于惠特曼作品的论文。惠特曼开美国一代诗风,无论在诗歌的内容上或形式上都进行了巨大的革新。他的诗作早在二十年代就已介绍到我国,但对他的诗的... 详细信息

本文是我院77级毕业生薛重在昆明师范学院英美文学专业硕士研究生班学习期间所写的一篇关于惠特曼作品的论文。惠特曼开美国一代诗风,无论在诗歌的内容上或形式上都进行了巨大的革新。他的诗作早在二十年代就已介绍到我国,但对他的诗的研究多偏在思想意义方面。本文集中探讨了惠特曼的代表作《草叶集》中最长、最著名的诗篇《自己之歌》的象征和结构,从艺术形式和技巧方面进行研究。文章从象征和结构在涛歌中的一般意义出发,进而论述《自己之歌》的象征和结构技巧及其与内容的关系。文章最后还谈到惠特曼《自己之歌》所体现的艺术上的革新对世界诗歌,特别是我国新诗的深刻影响。

关键词：惠特曼《草叶集》 SONG OF MYSELF SYMBOL AND STRUCTURE OF 《自己之歌》

Synthesis and Self-assembling of Coumarin-based Low-molecular Weight Organogelators

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Chemical Research in Chinese Universities 2009年第2期25卷 178-182页

作者： CHEN Guo-jun xue Peng-chong LU Ran SONG Dong-po BAO Chun-yan XU Ting-hua ZHAO Ying-ying College of Chemistry Jilin University Changchun 130012 P. R. China

Four coumarin derivatives（4a-4d） with different alkoxy chains were synthesized. It was found that compound 4d showed a better gelation ability than the other compounds, for example, it could self-assemble into organogels in various organic fluids via ultrasound treatment or heating-cooling process, whereas compound 4c could only gel in a few mixed solvents and compounds 4a, 4b could not form organogel. The results from fluorescent and FT-IR spectra indicate that π-π interaction had an effect on the formation of the organogels of compound 4d besides H-bonding and van der Waals interaction, which were the driving forces for the self-assembling of compound 4c in gel state. The gel of compound 4d in toluene could emit strong fluorescence under UV irradiation and the [2＋2] cyclo-addition was suggested by ^1H NMR and fluorescence spectroscopy. This light-sensitive organogel might find application in optical materials.

关键词： Coumarin Organogelator Self-assembling [2+2] Cyclo-addition

Synthesis, crystal structure and magnetic properties of [Cu（cyclm）]2+-based one-dimensional chain complexes

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Synthesis, crystal structure and magnetic properties of [Cu...

Construction and evaluation of anti-gastrin immunogen based on P64K protein

第七届全国物理无机化学学术会议

作者： chong-chong xue Hong-Yan Zhang Dao-Peng Zhang College of Chemical Engineering Shandong University of Technology

＜正＞The coordination chemistry of Cu（II）involved in an octahedral environment formed by four chemically equinoctial ligands and two axial ones is notable due to its marked Jahn-Teller effect along the axial *** has been known,tetramine ligands,both open-chain and macrocyclic,are the basis of an extraordinary amount of coordination chemistry,[1-5]for which the important properties is that they serve

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World Journal of Gastroenterology 2006年第13期12卷 2040-2046页

作者： Xiang-Hua Xiong Hong-Liang Zhao chong xue Wei Zhang Bing-Fen Yang xue-Qin Yao Zhi-Min Liu Department of Microbiologic Engineering Beijing Institute of Biotechnology Beijing 100071 China

AIM： To construct two kinds of anti-gastrin immunogen based on P64K protein from Neisseria rneningitids and to compare their immunogenic effect. METHODS： G17P64K gene was cloned and ligated into pET28a plasmid, then transformed into BL21（DE3）. After inoculation of LB medium and IPTG induction, the recombinant protein was solubly expressed at a high level. The purification of G17P64K fusion protein was similar to that of P64K. An initial step of purification consisting of 30% saturated ammonium sulfate precipitation was done, Additional fine optimizations included phenyl-sepharose, G200 Sephadex gel filtration and Q-sepharose anion exchanger chromatography. Highly purified protein was obtained and sequenced at the N-terminal amino acid residues. Polypeptide was synthesized by Fmoc solid phase chemical method and cross-linked to carrier protein P64K and DT mutant by MBS method and then the rabbit anti-gastrin 17 antibody was prepared by immunizing rabbit with cross-linked and fused protein. The titer and the activity in vitro of antibody were assessed. RESULTS： G17P64K gene and the recombinant bacteria were obtained. After four steps purification, protein sample that has the purity above 90% was achieved. At the 84^th day after the first immunization, the titer of antibody against cross-linked protein reached 51 200. Evaluation of the antibody in vitro manifested that it had a high inhibitory activity on the growth of tumor cell SW480. CONCLUSION： The P64K-polypeptide cross-linked immunogen immunized rabbit and achieved a higher titer antibody against gastrin 17 than the G17P64K fusion protein immunogen, which could inhibit the growth of the tumor cell SW480.

关键词： Gastrin P64K protein therapeutic vaccine

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信息代数紧扩张的一种新方法(英文)

数学杂志 2014年第4期34卷 610-616页

作者：管雪冲江苏师范大学数学与统计学院

本文研究了从一般信息代数获取紧信息代数的一种新方法.通过在一个信息代数的定向集族上建立一个等价关系,证得相应的等价类全体之集关于联合和聚焦两种运算构成了原信息代数的一个紧扩张.

关键词：信息代数紧信息代数双小于关系紧扩张

Secretory expression and characterization of a recombinant-deleted variant of human hepatocyte growth factor in Pichia pastoris

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World Journal of Gastroenterology 2005年第45期11卷 7097-7103页

作者： Zhi-Min Liu Hong-Liang Zhao chong xue Bing-Bing Deng Wei Zhang Xiang-Hua Xiong Bing-Fen Yang xue-Qin Yao Department of Microbiological Engineering Beijing instituteof Biotechnology 20 Dongdajie Street Fengtai District Beijing100071 China

AIM： To study the secretory expression of human hepatocyte growth factor （hdHGF） gene in Pichia pastoris. METHODS： The full-length gene of human cDNA encoding the deleted variant of hdHGF was cloned by RT-PCR and overlapping-fragment PCR technique using mRNA of human placenta as a template. The cloned hdHGF cDNA was inserted into the Escherichia coilyeast shuttle vector of pPIC9. The constructed plasmid, pPIC9-hdHGF, was transformed into the GSl15 cells of the methylotrophic yeast, P pastoris, using a chemical method. The Hut ＋ transformants were screened to obtain high-expression strains by the test and analysis of expressed products of shake-flask culture. A secretory form of rhdHGF was made with the aid of the leader peptide sequence of Saccharomyces cerevisiae α-factor. RESULTS： The expressed products, which showed a band of molecular mass of about 80 ku, were observed on 15% SDS-PAGE and identified by Western blotting and N-terminal amino acid sequencing. In the high cell density culture of 5 L fermentor by fed-batch culture protocol, the cell biomass was reached at approximately 135 g （DCW）/L. The productivity of secreted total supernant protein concentration attained a high-level expression of more than 8.0 g/L and the ratio of rhdHGF band area was about 12.3% of the total band area scanned by SDS-PAGE analysis, which estimated that the product of rhdHGF was 500-900 mg/***： The P pastoris system represents an attractive tool of generating large quantities of hdHGF for both research and industrial purposes.

关键词： Hepatocyte growth factor Pichia pastoris Secretory expressing

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肾移植术后钙调神经素抑制剂的减量和免除

中国医学科学院学报 2009年第3期31卷 280-283页

作者：薛翀纪志刚中国医学科学院北京协和医学院北京协和医院泌尿外科北京100730

钙调神经素抑制剂(CNI)在肾移植抗排异反应治疗中发挥重要作用,但其肾毒性被认为影响移植肾的长期存活。一些CNI节减方案(如CNI免用或减量)的应用显示其结果与标准剂量CNI方案相当,关于平衡CNI效用与毒性的研究仍在继续中。

关键词：肾移植钙调神经素抑制剂慢性移植物肾病急性排异反应