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Effects of alcohol on digestion,absorption and metabolism of sea cucumber saponins in healthy mice

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Food Science and Human Wellness 2024年第1期13卷 137-145页

作者： Wenxian Dang Rong Li Jinyue Yang changhu xue Qingrong Huang Yuming Wang Tiantian Zhang College of Food Science and Engineering Ocean University of ChinaQingdao 266003China Laboratory for Marine Drugs and Bioproducts Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Department of Food Science Rutgers State University New BrunswickNJ 08901USA

Sea cucumber saponins have attracted more attention in recent years due to biological *** is a popular practice to soak sea cucumber in Baijiu at home and being applied to industrial manufacturing in ***,knowledge of the effect of alcohol on the absorption and metabolism of sea cucumber saponins is *** effects of alcohol on digestion,absorption and metabolism of sea cucumber saponins in BALB/c mice were investigated after gavage and tail intravenous *** results showed that the content of saponins in serum and liver was significantly higher under the influence of alcohol than that in the control group after oral *** promoted the absorption of sea cucumber saponins prototype as well as inhibited the process of saponins being transformed into deglycositic metabolites in the small ***,sea cucumber saponins remained in circulation for a long time and alcohol slowed down the clearance of sea cucumber saponins under the influence of alcohol after intravenous *** confirmed the feasibility of marinating sea cucumber in Baijiu to improve the efficacy of saponins and provides an important theoretical basis for the utilization of sea cucumber and the development of sea cucumber liquor.

关键词： Holothurin A Echinoside A Alcohol Absorption Metabolism

Trimethylamine N-oxide generation process was influenced by the proportion and source of macronutrients in the diet

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Food Science and Human Wellness 2024年第2期13卷 649-658页

作者： Chengcheng Wang xuefeng Duan Xiaoyue Li Jinyue Yang changhu xue Teruyoshi Yanagita Tiantian Zhang Yuming Wang College of Food Science and Engineering Ocean University of ChinaQingdao 266003China Laboratory for Marine Drugs and Bioproducts Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Laboratory of Nutrition Biochemistry Department of Applied Biochemistry and Food ScienceSaga UniversitySaga 840-8502Japan

Trimethylamine N-oxide(TMAO)is a risk factor of various chronic diseases,which was produced by metabolism from precursors to trimethylamine(TMA)in gut and the oxidation from TMA in *** TMA generation was influenced by diet,mainly due to the rich TMAO precursors in ***,it was still unclear that the effects of different proportion and source of macronutrients in different dietary pattern on the production process of ***,the generation of TMA from precursors and TMAO from TMA was determined after single oral choline chloride and intraperitoneal injection TMA,respectively,in mice fed with carbohydrates,proteins and fats in different proportion and *** results suggested that the generation of TMAO was increased by low non-meat protein and high fat via enhancing the production of TMAO from TMA,and decreased by plant protein and refined sugar via reducing TMA production from precursors in gut and TMAO transformation from TMA in *** high fat and high sugar diets accelerating the development of atherosclerosis did not increase the production of TMAO,the risk factor for atherosclerosis,which indicated that the dietary compositions rather than the elevated TMAO level might be a more key risk factor for atherosclerosis.

关键词： Trimethylamine N-oxide(TMAO) Trimethylamine(TMA) Dietary composition Macronutrients Gut microbiota

Docosahexaenoic acid-acylated astaxanthin monoester enhances microglial autophagy for ameliorating amyloid-β load and cognitive deficits in models of Alzheimer’s disease

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Food Science and Human Wellness 2024年

作者： Xiaoxu Wang Bo Dong Yu Song Zhigao Wang Yuming Wang Jie Xu changhu xue State Key Laboratory of Marine Food Processing & Safety Control College of Food Science and Engineering Ocean University of China College of Marine Life Sciences Ocean University of China Qingdao National Laboratory for Marine Science and Technology

Autophagy directly regulates the amyloid-peptides （Aβ） clearance， and its dysfunction occurs in the early pathogenesis of Alzheimer’s disease （AD）. We previously reported that docosahexaenoic acid-acylated astaxanthin monoester （AST-DHA） showed neuroprotection against AD pathology. However， its in-depth mechanism and autophagic responses in AD brains are poorly understood. Herein， SH-SY5Y cells overexpressing the APP gene were established to preliminarily evaluate the actions of AST-DHA on reducing Aβ1-42levels and regulating autophagy. In microglial BV2 cells， AST-DHA and free astaxanthin （F-AST） recovered p62 and LC3II/I levels， and restored autophagy flux by rescuing the late phase of microglial autophagy. Notably， autophagic inhibitor bafilomycin A1 blunted the abilities of AST-DHA to reduce Aβ1-42and fibral Aβ， suggesting that AST-DHA probably promoted Aβ clearance in a microglial autophagy-dependent manner. Further studies in APP/PS1 mice verified that dietary AST-DHA and F-AST promoted Aβ phagocytosis via microglial autophagy. Significant decreases of Iba1 and p62 levels were observed around Aβ plaque in the hippocampus and cortex using triple fluorescence staining. Furthermore， AST-DHA exhibited superior performance over F-AST in restoring autophagic dysfunction， ameliorating Aβ burden and cognitive deficit. Our findings suggest a possible mechanism of AST-DHA in improving AD by which it restores microglial autophagy to facilitate cerebral Aβ clearance. It supports the future application of AST-DHA as an autophagic regulator in maintaining brain function.

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Sea cucumber derived sulfated sterols alter glucose metabolism by promoting gluconeogenesis and reducing glycogenesis in healthy mice

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Food Science and Human Wellness 2024年

作者： Shanyun Yu Teng Wang Yingcai Zhao Xiaoyue Li changhu xue Yuming Wang Tiantian Zhang SKL of Marine Food Processing & Safety Control College of Food Science and Engineering Ocean University of China Sanya Institute of Oceanography Ocean University of China

Sea cucumber derived sulfated sterols (SS) significantly ameliorated insulin resistance and decreased lipid accumulation compared to plant sterols. Interestingly， our recent study found that intervention with sea cucumber sulfated sterols could significantly increase blood glucose levels of healthy mice in the presence of glucose， while cholesterol sulfate， as one of sulfated sterols， did not have the same effect. However， the exact mechanism of SS on glucose metabolism is still unknown. In the present study， we investigated the potential mechanism by which SS influenced blood glucose homeostasis in healthy mice. Results showed that intervention with sea cucumber sulfated sterols did not affect the levels of hormones related to glucose metabolism， while led to a significant decrease in the synthesis of liver glycogen and muscle glycogen. Besides， the expression of proteins associated with the promotion of gluconeogenesis dramatically increased in the mice intervened with sea cucumber sulfated sterols. These findings suggested that sea cucumber sulfated sterols might change blood glucose metabolism in healthy mice by reducing glycogenesis and promoting gluconeogenesis.

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一种双层多糖水凝胶用于增强益生菌肠道靶向口服递送

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Engineering 2024年第3期34卷 187-194页

作者： Wen-Can Huang Wenjie Wang Wei Wang Yanan Hao changhu xue Xiangzhao Mao State Key Laboratory of Marine Food Processing and Safety Control College of Food Science and EngineeringOcean University of ChinaQingdao 266404China. Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology Qingdao 266237China Qingdao Key Laboratory of Food Biotechnology Qingdao 266404China Key Laboratory of Biological Processing of Aquatic Products China National Light IndustryQingdao 266404China

Transplantation of probiotics to the intestine can positively regulate the gut microbiota,thereby promoting the immune system and treating various ***,the harsh gastrointestinal environment and short retention time in the gastrointestinal tract significantly limit the bioavailability and intestinal colonization of ***,we present a double-layer polysaccharide hydrogel(DPH)in the form of a double-layer structure composed of a carboxymethyl cellulose(CMCL)supramolecular inner layer and a dialdehyde alginate(DAA)cross-linked carboxymethyl chitosan(CMCS)outer *** doublelayer structure allows DPH to encapsulate and deliver probiotics in a targeted manner within the *** the stomach,the cage structure of the DPH is closed,and the outer layer absorbs surrounding liquids to form a barrier to protect the probiotics from gastric *** the intestine,the cage structure opens and disintegrates,releasing the ***,DPH endows probiotics with excellent intestine-targeted delivery,improved oral bioavailability,enhanced gastrointestinal tract tolerance,and robust mucoadhesion *** encapsulated probiotics exhibit almost unchanged bioactivity in the gastrointestinal tract before release,as well as improved oral *** particular,probiotics encapsulated by DPH exhibit 100.1 times higher bioavailability and 10.6 times higher mucoadhesion than free probiotics in an animal model 48 h *** addition,with a remarkable ability to survive and be retained in the intestine,probiotics encapsulated by DPH show excellent in vitro and in vivo competition with ***,DAA-mediated dynamic crosslinking not only maintains the overall integrity of the hydrogels but also controls the release timing of the ***,it is expected that encapsulated substances(probiotics,proteins,etc.)can be delivered to specific sites of the intestinal tract by means of DPH,by controlling the dynamic covalent crosslinking.

关键词： Polysaccharides Chitosan Hydrogels Oral delivery Intestine-targeted

Properties and Anti-Ultraviolet Activity of Gallic Acid-Chitosan-Gelatin Mixed Gel

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Journal of Ocean University of China 2022年第1期21卷 204-212页

作者： WANG Wenjie xue changhu MAO Xiangzhao College of Food Science and Engineering Ocean University of ChinaQingdao 266003China Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology Qingdao 266200China

Hydrogel has high water content and structural similarity with natural extracellular *** it has been widely studied and applied in the field of *** order to further develop multifunctional hydrogels,we prepared mixed gels with antiultraviolet *** study found that the addition of polysaccharides and polyphenols was beneficial to the rheological,me-chanical properties,and biological activity of the ***(CS)could significantly improve the viscoelasticity,hardness,gel strength,thermal stability and crystallinity of ***,the addition of gallic acid(GA)could not only provide significant cross-linking effect,improve gel properties and microstructure,but also improve the UV resistance of the mixed gel.

关键词： gallic acid chitosan gelatin gel properties UV resistance

一种内切-1,3-岩藻聚糖酶的乳酸菌表达及性质

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水产学报 2024年第7期48卷 169-177页

作者：李新雨陈广宁申晶晶陈环环薛长湖常耀光中国海洋大学食品科学与工程学院

岩藻聚糖是一种具有良好应用潜力的多糖，具备多种生理活性。内切-1,3-岩藻聚糖酶是岩藻寡糖制备的关键工具。然而，作用方式清晰的食品工业用岩藻聚糖酶的缺乏阻碍了该类酶的应用。本研究旨在获得来源于GH174家族的食品工业用内切-1,3... 详细信息

岩藻聚糖是一种具有良好应用潜力的多糖，具备多种生理活性。内切-1,3-岩藻聚糖酶是岩藻寡糖制备的关键工具。然而，作用方式清晰的食品工业用岩藻聚糖酶的缺乏阻碍了该类酶的应用。本研究旨在获得来源于GH174家族的食品工业用内切-1,3-岩藻聚糖酶Fun174Sb，并探究其作用方式。利用乳酸菌表达系统实现了一种内切-1,3-岩藻聚糖酶Fun174Sb的异源表达。结果显示，Fun174Sb对于来源于土耳其刺参的岩藻聚糖（Ht-FUC）具有降解能力，酶活性为1.90 U/mg。该酶在35～50°C、pH 7.5～8.5条件下显示出较高的活性，且具有良好的温度稳定性及pH稳定性。通过超高效凝胶排阻色谱串联质谱以及核磁共振技术阐明了Fun174Sb的酶解产物中主要寡糖的结构，从而推断出Fun174Sb切割HtFUC的Fucp2（OSO;3;-;）与Fucp2,4（OSO;3;-;）之间的α-1,3-糖苷键。研究表明，乳酸菌表达的Fun174Sb具有良好的生化性质以及清晰的作用方式。本研究为作用方式清晰的食品级岩藻聚糖的应用奠定基础。

关键词：岩藻聚糖酶乳酸菌刺参克隆表达生化性质作用方式

Cloning,Expressing and Characterizing a Novel Cold-Adapted Laminarinase from Marine Bacterium Wenyingzhuangia aestuarii OF219

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Journal of Ocean University of China 2023年第4期22卷 1034-1040页

作者： LI Jiajing xue changhu CHANG Yaoguang SHEN Jingjing ZHANG Yuying College of Food Science and Engineering Ocean University of ChinaQingdao 266003China Laboratory for Marine Drugs and Bioproducts Qingdao Marine Science and Technology CenterQingdao 266237China

Laminarinases reveal potential application in the field of food and *** this research,a novel GH16 family laminarinase,designated as Lam16A_Wa,was cloned from the genome of marine bacterium Wenyingzhuangia aestuarii OF219 and expressed in Escherichia ***16A_Wa demonstrates a relatively low optimal reaction temperature(35℃)and a cold-adapted *** optimal pH value is 6.0 and is stable in a broad pH range from 3.0 to 11.0.A glycomics strategy was employed to investigate the hydrolytic pattern of Lam16A_*** enzyme was confirmed as a random endo-acting glycoside *** minimum substrate was laminarin pentasaccharide,and the major final products are oligosaccharides,including disaccharide to *** Lam16A_Wa provides a novel and well-defined tool for the molecular tailoring of laminarin.

关键词： laminarinase laminarin glycomics GH16 family

不同温度胁迫条件下太平洋牡蛎挥发性化合物的变化

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现代食品科技 2024年第2期40卷 265-272页

作者：孟楠郝丽莉王昕岑宋雨丛培旭徐杰李兆杰薛长湖中国海洋大学食品科学与工程学院山东青岛266003 青岛大学营养与健康研究院山东青岛266071 青岛海洋科学与技术试点国家实验室山东青岛266237

为探究温度胁迫后太平洋牡蛎挥发性成分的变化,该研究采用感官评定、气相色谱-离子迁移谱(Gas Chromatography-Ion Mobility Spectroscopy,GC-IMS)、气相色谱-质谱联用(Gas Chromatography-Mass Spectrometer,GC-MS)结合多元统计分析的方法,对不同温度胁迫条件(12、22、32℃)的太平洋牡蛎挥发性成分进行了检测,同时测定了其存活率以及脂肪酸含量的变化。结果显示,胁迫6 d后,低温12℃组的存活率达到97.90%,n-3型多不饱和脂肪酸(Polyunsaturated Fatty Acid,PUFA)含量升高。感官评定、GC-IMS以及GC-MS结果显示辛醛等鱼腥味醛类成分含量下降,而呈紫罗兰气味的(E,Z)-2,6-壬二烯醛升至12.15%,提供了愉悦的气味。高温32℃胁迫后存活率降至26.00%,n-3 PUFA含量下降从46.07%下降至39.59%,同时饱和醛等腥味醛类含量显著上升,加重了太平洋牡蛎的腥味,导致牡蛎的品质严重下降。最后通过统计分析筛选6种关键成分:己醛、辛醛、(E,Z)-2,6-壬二烯醛、(E)-2-壬烯醛、(E,E)-2,4-庚二烯醛以及3-辛酮。温度对太平洋牡蛎的存活率、n-3 PUFA含量及挥发性成分有较大影响,该研究为提升太平洋牡蛎品质提供了有益参考。

关键词：太平洋牡蛎温度胁迫存活率脂肪酸挥发性成分