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The haplotype-resolved genome assembly of autotetraploid rhubarb Rheum officinale provides insights into its genome evolution and massive accumulation of anthraquinones

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Plant Communications 2024年第1期5卷 196-214页

作者： Hongyu Zhang Qiang He Longsheng Xing Ruyu Wang Yu Wang Yu Liu Qinghong Zhou xuanzhao Li Zheng Jia Ze Liu Yuqing Miao Tao Lin Wei Li Huilong Du School of Life Sciences Institute of Life Sciences and Green DevelopmentBasic Science Center for Biotic Interaction in HebeiHebei UniversityBaoding 071000China College of Horticulture China Agricultural UniversityNo.2 Yuanmingyuan West RoadHaidian DistrictBeijing 100193China

Rheum officinale,a member of the Polygonaceae family,is an important medicinal plant that is widely used in traditional Chinese ***,we report a 7.68-Gb chromosome-scale assembly of *** with a contig N50 of 3.47 Mb,which was clustered into 44 chromosomes across four homologous *** genomics analysis revealed that transposable elements have made a significant contribution to its genome evolution,gene copy number variation,and gene regulation and expression,particularly of genes involved in metabolite biosynthesis,stress resistance,and root *** placed the recent autotetraploidization of *** at~0.58 mya and analyzed the genomic features of its homol-ogous *** no dominant monoploid genomes were observed at the overall expression level,numerous allele-differentially-expressed genes were identified,mainly with different transposable element insertions in their regulatory regions,suggesting that they functionally diverged after *** genomics,transcriptomics,and metabolomics,we explored the contributions of gene family amplification and tetraploidization to the abundant anthraquinone production of ***,as well as gene expression patterns and differences in anthraquinone content among *** report of-fers unprecedented genomic resources for fundamental research on the autopolyploid herb *** and guidance for polyploid breeding of herbs.

关键词： Rheum officinale genomics comparative genomics polyploid anthraquinone biosynthesis

Microbial Degradation of Quinoline: Kinetics Study With Burkholderia picekttii

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Biomedical and Environmental Sciences 2004年第1期17卷 21-26页

作者： JIAN-LONGWANG WEI-ZHONG xuanzhao LaboratoryofEnvironmentalTechnology INETTsinghuaUniversityBeijing100084China SchoolofEnvironmentalScience PekingUniversityBeijing100871China

Objective To investigate the kinetics of quinoline biodegradation by Burkholderia pickttii, a Gram negative rod-shaped aerobe, isolated in our laboratory. Methods HPLC (Hewlett-Packard model 5050 with an UV detector) was used for the analysis of quinoline concentration. GC/MS method was used to identify the intermediate metabolites of quinoline degradation. Results The biodegradation of quinoline was inhibited by quinoline at a high concentration, and the degradation process could be described by the Haldane model. The kinetic parameters based on Haldane substrate inhibition were evaluated. The values were v = 0.44 h-1,Ks=166.7 mg/L, Ki= 650 mg/L, respectively. The quinoline concentration to avoid substrate inhibition was inferred theoretically and determined to be 329 mg/L. Conclusion The biodegradation of quinoline conforms to the Haldane inhibition model and the main intermediate metabolite of quinoline biodegradation is 2-hydroxy-quinoline.

关键词： Quinoline Microbial degradation Burkholderia pickttii Kinetics Substrate inhibition Intermediate metabolite

miR-339-5p通过靶向调节p53表达影响脑胶质瘤细胞的转移活性分析

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实用癌症杂志 2024年第3期39卷 360-364页

作者：柳毅高绚照常文广河南省新乡市中心医院 453000

目的研究miR-339-5p对脑胶质瘤U87细胞的影响,并探讨相关机制。方法将U87细胞分为4组:空白组、miR-339-5p mimic组、si-p53组、miR-339-5p mimic+si-p53组。通过MTT实验检测细胞增殖率;通过Transwell实验检测U87细胞侵袭能力;通过流式... 详细信息

目的研究miR-339-5p对脑胶质瘤U87细胞的影响,并探讨相关机制。方法将U87细胞分为4组:空白组、miR-339-5p mimic组、si-p53组、miR-339-5p mimic+si-p53组。通过MTT实验检测细胞增殖率;通过Transwell实验检测U87细胞侵袭能力;通过流式细胞术检测细胞凋亡率;通过荧光素酶报告基因实验检测miR-339-5p与p53的靶向关系;通过实时荧光定量聚合酶链反应和蛋白免疫印迹分析miR-339-5p和p53的表达水平。结果miR-339-5p mimic组和miR-339-5p mimic+si-p53组细胞的miR-339-5p表达水平高于空白组和si-p53组,miR-339-5p mimic组细胞的p53 mRNA和蛋白水平均高于空白组、si-p53组和miR-339-5p mimic+si-p53组(P<0.05)。miR-339-5p mimic和p53-WT共转染的细胞荧光素酶活性明显增加(P<0.05)。miR-339-5p mimic组细胞增殖率和细胞侵袭数量低于空白组、si-p53组和miR-339-5p mimic+si-p53组,同时miR-339-5p mimic组细胞凋亡率高于空白组、si-p53组和miR-339-5p mimic+si-p53组(P<0.05)。结论miR-339-5p能够抑制脑胶质瘤U87细胞的增殖和侵袭,并促进胶质瘤U87细胞凋亡,同时这一过程与靶向激活p53相关。

关键词： miR-339-5p p53 脑胶质瘤转移

Carbon dots modified nanoflower petals with super enhanced nitrogen electro-reduction efficiency

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Chinese Chemical Letters 2023年第6期34卷 406-411页

作者： Yang Zhou xuanzhao Lu Yu-chung Chang Yanwen Ma Linlin Wang Jianrong Zhang Junjie Zhu State Key Laboratory for Organic Electronics and Information Displays&Institute of Advanced Materials IAM Nanjing University of Posts&TelecommunicationsNanjing 210023China State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China School of Mechanical and Materials Engineering Washington State UniversityPullmanWA 99164United States

Nitrogen electro-reduction reaction(NERR)is a promising alternative method for ammonia production to the Haber-Bosch approach due to mild reaction conditions and free harmful by-product emission.A formidable challenge in bringing NERR closer to the practical application is developing an electrocatalyst which can simultaneously improve the Faraday efficiency and reduce the reaction ***,we fabricated a catalyst of nitrogen-doped carbon dots modified copper-phosphate nanoflower petals(Cu Po-NCDs NF)via a self-assembly *** flower structure endowed the Cu Po-NCDs NF with large specific surface area,and thus enabled more active sites to be *** particular,we demonstrated that the NCDs modified Cu Po petals with flower-like structure can accelerate the interfacial proton-electron transfer,suppressing the competing hydrogen evolution reaction and promoting the desired NERR ***,for the CuPo-NCDs NF catalyzed NERR,the FE_(NH_(3))and the reaction potential both were boosted,the resultant energy efficiency of NERR reached a record-breaking value of 56.5%,and the NH_(3)yield rate increased by 7 times compared to *** study provides a novel catalyst with a new pathway to boost the NERR.

关键词： Nitrogen-doped carbon dots Nano-flower Self-assembly Interfacial proton-electron transfer Nitrogen electro-reduction

2018—2020年上海市闵行区腹泻病监测结果分析

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公共卫生与预防医学 2024年第1期35卷 79-82页

作者：方辉张悦张兆文陈龙徐智寅张烜昭上海市闵行区莘庄社区卫生服务中心预防保健科上海201199 上海市闵行区疾病预防控制中心传染病防制科上海201101

目的分析上海市闵行区腹泻病的流行病学和病原学特征,为制定腹泻病的防控策略提供科学依据。方法对上海市闵行区2018—2020年腹泻病综合监测医院的腹泻病例进行流行病学调查,根据监测医院肠道门诊量在符合监测病例要求的病例队列中,按... 详细信息

目的分析上海市闵行区腹泻病的流行病学和病原学特征,为制定腹泻病的防控策略提供科学依据。方法对上海市闵行区2018—2020年腹泻病综合监测医院的腹泻病例进行流行病学调查,根据监测医院肠道门诊量在符合监测病例要求的病例队列中,按照固定间隔比例采用系统抽样法采集病例粪便标本进行病原学检测,分析其病原构成及流行病学特征。结果721例标本中检出阳性标本307例,阳性率42.58%;检出细菌最多的为副溶血性弧菌39份,检出病毒最多的为诺如病毒GII型75份,两者各占细菌和病毒阳性总数的36.11%和41.44%。各年龄组均有检出阳性病例,31-40岁共检出阳性81例(26.38%),检出率最高;阳性检出率性别间差异无统计学意义(X^(2)=1.95,P=0.16)。检出阳性病例呈现出冬季和春季两个高峰,其中细菌三季度最高,病毒一季度最高,细菌和病毒的混合感染最多时间为二季度。结论上海市闵行区2018—2020年腹泻病由多种病原引起,且具有明显季节流行特征,在不同病原体流行前应开展针对性防控措施,减少腹泻病发生。

关键词：腹泻病病原谱监测

Differential Diagnosisand Treatmentof Rudimentary Uterine Hornand Blind Uterine Hornin Puberty

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Journal of Reproduction and Contraception 2004年第2期15卷 115-120页

作者： WeiXU Yi-xuanzhao Hai-xuSUN WeiYU Xiao-yongLI Xiu-meiLI Shou-rouLI DepartmentofObstetricsandGynecology SecondHospitalofNormanBethuneCollegeofJilinUniversityChangchun130041China

Objective To investigate the diagnostic methods of pubertal uterine malformations in nongestational stage Method Hysteroscopy combined with transabdominal ultrasonography was used to diagnose and differentiate rudimentary uterine horn and blind uterine horn in puberty. Results The accuracy of the two diagnostic methods performed simultaneously was valuable in the diagnosis of pubertal unicornuate uterus with blind uterine horn in 3 cases and rudimentary uterine horn in 2 cases. Conclusion Hysteroscopy combined with transabdominal ultrasonography was reliable in diag- nosing uterine malformations and especially effective in treating maiden with rudimentary horn and blind horn of uterus.

关键词： hysteroscopy ultrasonography uterine malformations rudimentary uterine horn blind uterine horn

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一种基于语义的服务发现算法

计算机工程 2006年第3期32卷 102-104页

作者：张大陆王炫召同济大学计算机科学与技术系上海200092

提出了基于语义Web的技术。首先要定义一个服务本体,服务提供者通过服务本体描述服务信息,并以语义Web的形式来发布。用户可以定义查询条件来查询自己所需要的服务。查询条件可以多种形式定义,如语义Web形式和三元组形式。这需要有一个... 详细信息

提出了基于语义Web的技术。首先要定义一个服务本体,服务提供者通过服务本体描述服务信息,并以语义Web的形式来发布。用户可以定义查询条件来查询自己所需要的服务。查询条件可以多种形式定义,如语义Web形式和三元组形式。这需要有一个算法来处理用户查询,能够处理语义信息并进行推理,对用户的查询和所提供的服务进行匹配,并返回查询结果。文章研究了语义Web的推理机制,并提出了一种用于快速和准确的服务发现的匹配算法。

关键词：服务发现语义Web 标志语言语义推理匹配算法

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跨越断层埋地管线地震反应数值分析

地震工程与工程振动 2006年第3期26卷 186-192页

作者：林均岐胡明祎申选召中国地震局工程力学研究所黑龙江哈尔滨150080

跨越断层埋地管线在地震中的破坏是非常严重的,地震本身和管土相互作用体系中都存在很多不确定性因素,所以管线在断层运动过程中反应比较复杂。本文利用有限元理论和数值模拟手段,建立了管土作用模型,采用非线性接触问题研究方法详细地... 详细信息

跨越断层埋地管线在地震中的破坏是非常严重的,地震本身和管土相互作用体系中都存在很多不确定性因素,所以管线在断层运动过程中反应比较复杂。本文利用有限元理论和数值模拟手段,建立了管土作用模型,采用非线性接触问题研究方法详细地分析了管线由断层运动而产生的反应,对影响管线的各种因素进行了分析,包括位错量、跨越角度、断层运动形式、埋设深度、初始轴向力、断层裂缝宽度、填覆土质和管径。通过研究,得到一些初步结论。

关键词：断层埋地管线地震反应数值模拟

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基于区块链的业务流程互操作服务框架

计算机集成制造系统 2021年第9期27卷 2508-2516页

作者：唐玄昭余阳吴荆璞潘茂林中山大学数据科学与计算机学院广东广州510006

业务流程管理系统(BPMS)的出现极大地提高了企业执行流程的效率。由于企业间缺乏信任且跨引擎业务流程之间缺乏互操作规范,导致跨组织流程协同的应用受到许多制约。针对该问题提出一种支持BPMS的互操作框架,该框架引入区块链技术作为互... 详细信息

业务流程管理系统(BPMS)的出现极大地提高了企业执行流程的效率。由于企业间缺乏信任且跨引擎业务流程之间缺乏互操作规范,导致跨组织流程协同的应用受到许多制约。针对该问题提出一种支持BPMS的互操作框架,该框架引入区块链技术作为互操作智能合约的协作平台,构建了支持业务流程建模标记语言(BPMN)的互操作服务,支持企业间基于BPMS的多方业务流程协同,并保证协同的可信性,从而提高企业间协同的自动化水平和信任度。该服务的引入确保了互操作的过程符合预定的顺序,且交互记录不可修改。使用工作流管理联盟(WfMC)的Wf-XML 2.0协议来支持BPMS间的互操作能力,从而减少企业间使用的工作流引擎及标准不同所带来的复杂性。最后,通过一个经典的案例对WfMC提出的4种常见互操作场景进行了讨论,表明了该框架对多方业务流程基于BPMS来自动地完成可信协同的支持。

关键词：工作流互操作业务流程管理系统 Wf-XML 2.0协议区块链智能合约