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Science China Chemistry 2025年第1期 360-368页

作者： Zheng Liu Changyu Zhang Zipeng Li Wanying Ma Jia Liu Xiang Xia ning xu Wen Sun Jianjun Du Jiangli Fan Xiaojun Peng State Key Laboratory of Fine Chemicals Dalian University of Technology ningbo Institute of Dalian University of Technology

Endoplasmic reticulum(ER) viscosity has emerged as a new potential biomarker for hepatic fibrosis(HF). Herein, we report the first ER-targeting viscosity-responsive near-infrared(NIR) fluorescent probe HBT-PP for investigations of HF *** twisted intramolecular charge transfer(TICT) and the excited state intramolecular proton transfer(ESIPT) are combined to endow HBT-PP with the selective response towards viscosity(～62-fold) and a super large stokes shift(～320 nm). HBT-PP mainly targets organelle ER and is able to image the drug-or alcohol-induced viscosity elevation in living cells. Significantly,HBT-PP was successfully applied to visualize the gender difference in HF and the protective role of estrogen in restraining HF progression. Of note, after 10 min of stain with HBT-PP, HF lesions showed a stage-based response, indicating the potential of this probe in fast and early diagnosis of HF. These results clearly demonstrated the promising application of HBT-PP as a new tool for exploring ER viscosity-involved physiological and pathological processes.

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circKEAP1通过影响miR-661/LIF轴促进骨肉瘤细胞增殖和迁移

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中国病理生理杂志 2022年第10期38卷 1856-1865页

作者：黄晓文张能华陈兴英袁梅费春霞徐屹宁浙江中医药大学附属嘉兴中医院检验科浙江嘉兴314001 浙江大学医学院附属邵逸夫医院骨科浙江杭州310020

目的:探讨环状RNA circKEAP1通过影响微小RNA-661(miR-661),进而调控白血病抑制因子(LIF)基因促进骨肉瘤细胞凋亡和迁移的作用及其机制。方法:采用RT-qPCR探究不同骨肉瘤细胞系和成骨细胞hFOB1.19对照中circKEAP1的表达量,并通过一代测... 详细信息

目的:探讨环状RNA circKEAP1通过影响微小RNA-661(miR-661),进而调控白血病抑制因子(LIF)基因促进骨肉瘤细胞凋亡和迁移的作用及其机制。方法:采用RT-qPCR探究不同骨肉瘤细胞系和成骨细胞hFOB1.19对照中circKEAP1的表达量,并通过一代测序验证其成环剪切位点。通过PCR验证circKEAP1在互补DNA与基因组DNA中的表达,并探究其在放线菌素D作用下的降解速率。构建circKEAP1的小干扰RNA,敲减circKEAP1后通过CCK-8、流式细胞术和细胞迁移实验评估骨肉瘤细胞143B活力、凋亡和迁移的改变。萤光素酶报告基因实验评估circKEAP1与miR-661的结合情况。通过CCK-8、流式细胞术和细胞迁移实验评估miR-661抑制剂对circKEAP1敲减的影响。转录组测序结合生物信息学分析预测circKEAP1的下游靶基因,并通过萤光素酶报告基因实验评估miR-661与LIF的结合情况。通过CCK-8、流式细胞术和细胞迁移实验评估LIF过表达对circKEAP1敲减的影响。结果:RT-qPCR结果显示,与成骨细胞hFOB1.19相比,多种骨肉瘤细胞系高表达circKEAP1(P<0.01)。circKEAP1存在于互补DNA中,而不存在于基因组DNA中,并且相对于其母基因KEAP1具有更好的稳定性。敲减circKEAP1显著抑制了143B细胞的生长,促进其凋亡,增加其迁移能力(P<0.01)。萤光素酶报告基因实验结果显示circKEAP1能与miR-661结合(P<0.01)。miR-661抑制剂可逆转敲减circKEAP1介导的细胞活力降低、凋亡增加和迁移能力减弱(P<0.01)。转录组测序结合生物信息学分析锁定下游靶基因LIF。萤光素酶报告基因实验结果显示miR-661能结合LIF(P<0.01)。RT-qPCR结果显示,敲减circKEAP1显著下调了LIF的mRNA水平(P<0.01)。在143B细胞中过表达LIF可逆转敲减circKEAP1介导的细胞活力降低、凋亡增加和迁移能力减弱(P<0.01)。结论:环状RNA circKEAP1能吸附miR-661,进而调控其下游靶基因LIF。circKEAP1-miR-661-LIF轴可能在骨肉瘤细胞的发生与进展中发挥重要作用。

关键词： circKEAP1 骨肉瘤细胞凋亡细胞迁移 miR-661/LIF轴

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神经网络方法在变电站消防管理的应用研究

消防科学与技术 2020年第12期39卷 1770-1772页

作者：李富强瞿航徐宁一朱文超国网浙江省电力有限公司宁波供电公司浙江宁波315000

为了提升变电站消防管理科学化水平,实现视频图片火焰特征提取及识别定位。接入视频监控码流数据,构建基于卷积神经网络的火焰识别模型,进行实时识别火焰特征并预警预报。实验表明,该方法能够自动提取火焰特征,有效提高复杂背景下的火... 详细信息

为了提升变电站消防管理科学化水平,实现视频图片火焰特征提取及识别定位。接入视频监控码流数据,构建基于卷积神经网络的火焰识别模型,进行实时识别火焰特征并预警预报。实验表明,该方法能够自动提取火焰特征,有效提高复杂背景下的火焰识别的准确率,具有良好的鲁棒性和泛化能力,在变电站消防管理中有较大应用前景。

关键词：卷积神经网络火焰识别变电站消防管理

二氧化碳倍增对植物叶片^(15)N自然丰度的影响

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地球科学与环境学报 2024年第3期46卷 298-306页

作者：李蕾吴明君林冰艳孙嫣然徐艺宁汪旭明巩晓颖福建师范大学地理科学学院/碳中和未来技术学院福建福州350007

大气CO_(2)浓度上升通常会提高植物生产力并伴随叶片氮含量的下降。然而大气CO_(2)如何影响叶片^(15)N丰度及其相关机理还不清楚。以小麦和向日葵为实验材料,测定了两个CO_(2)浓度(410与820μmol•mol^(-1))处理下叶片的氮同位比值(δ^(1... 详细信息

大气CO_(2)浓度上升通常会提高植物生产力并伴随叶片氮含量的下降。然而大气CO_(2)如何影响叶片^(15)N丰度及其相关机理还不清楚。以小麦和向日葵为实验材料,测定了两个CO_(2)浓度(410与820μmol•mol^(-1))处理下叶片的氮同位比值(δ^(15)N)和氮含量。结果表明:小麦和向日葵叶片氮含量随CO_(2)浓度升高呈下降趋势,然而δ^(15)N对CO_(2)浓度倍增的响应存在差异。在高CO_(2)浓度处理下小麦叶片δ^(15)N显著下降6.5‰,而向日葵叶片δ^(15)N小幅上升2.1‰,且叶片和地上部生物量显著增加。基于此,小麦的氮营养特征符合氮同化受限假说,而向日葵符合稀释效应假说。小麦叶片δ^(15)N随叶龄或者细胞年龄的增加而显著下降,因此在利用^(15)N来研究植物氮代谢时需要区分叶龄的影响。整合分析结果表明,CO_(2)浓度升高导致非豆科C_(3)植物的δ^(15)N显著下降达0.3‰,与小麦的研究结果相符。综上所述,限制硝态氮同化是CO_(2)影响植物氮代谢和^(15)N丰度的重要机制。

关键词：植物氮代谢氮同化 ^(15)N自然丰度 CO_(2)浓度叶龄氮利用效率氮含量稀释效应

Clinicopathological differences between patients with schistosomal appendicitis and non schistosomal appendicitis:A retrospectively study of past ten years

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World Journal of Clinical Cases 2025年第2期13卷 24-32页

作者： Xiao-yi Wang Yao Hao Zi-Jian Wang Xiu-Liang xu Jiang-Hua Yang Department of Infectious Diseases The First Affiliated of Wannan Medical CollegeWuhu 241001Anhui ProvinceChina

BACKGROUND Chronic schistosomiasis causes multiple organ and multiple system diseases,especially the digestive *** eggs are mainly deposited in the stomach,liver and colorectal,but a few eggs are deposited in the appendix and cause *** present,there are few studies on schistosomal *** To explore the differences in epidemiological,clinical and pathological characteristics between schistosomal appendicitis and non-schistosomal appendicitis over the past decade in order to assess the impact of schistosomiasis on *** The differences of general data,clinical data and laboratory examination data of patients with appendicitis from October 2013 to October 2023 were retrospectively *** patients were divided into two groups for *** were 136 patients in schistosomal appendicitis group and 5418 patients in non-schistosomal appendicitis *** Schistosomal appendicitis accounted for 2.45%of all patients with appendicitis,and the annual proportion in the past decade was 2.2%,2.9%,1.8%,1.9%,3.4%,3.1%,1.9%,1.6%,3%,2.6%,*** prevalence of schistosomal appendicitis was middle-aged and elderly males,with an average age of 61.73±15.335 *** main population of non-schistosomal appendicitis was middle-aged men,with an average age of 35.8±24.013 years(P<0.001).The distribution of pathological types of appendicitis was different between the two groups(P<0.001).The incidence of acute suppurative appendicitis in non-schistosomal appendicitis was higher than that in schistosomal appendicitis[odds ratio(OR)=0.504;95%confidence interval(CI):0.349-0.728;P<0.001].The proportion of acute attack of chronic appendicitis in schistosomal appendicitis was higher than that in non-schistosomal appendicitis(OR=2.614;95%CI:1.815-3.763;P<0.001).The proportion of schistosomal appendicitis patients complicated with colorectal cancer was higher than that of nonschistosomal appendicitis patients(OR=5.087;95%CI:1.427-

关键词： Schistosomal appendicitis Schistosomiasis Appendix Colorectal cancer Clinicopathological characteristics

卵巢黏液性囊腺瘤患者的磁共振成像影像学特征及临床应用价值分析

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中国妇幼保健 2024年第10期39卷 1907-1910页

作者：陆荣成许莉徐忆宁邓冬平胡赟湖州市中医院放射科浙江湖州313000 湖州市妇幼保健院妇产科湖州市中心医院放射科湖州市第一人民医院放射科湖州市中医院病理科

目的探讨卵巢黏液性囊腺瘤患者的磁共振成像(MRI)影像学特征及对良性黏液性囊腺瘤和交界性黏液性囊腺瘤的诊断价值。方法选取2020年4月-2023年8月湖州市中医院收治的92例卵巢黏液性囊腺瘤患者,依据病理结果分为良性组(50例)和交界组(4... 详细信息

目的探讨卵巢黏液性囊腺瘤患者的磁共振成像(MRI)影像学特征及对良性黏液性囊腺瘤和交界性黏液性囊腺瘤的诊断价值。方法选取2020年4月-2023年8月湖州市中医院收治的92例卵巢黏液性囊腺瘤患者,依据病理结果分为良性组(50例)和交界组(42例),对所有患者进行MRI检查。分析两组患者MRI影像学特征和信号强度;比较单房和多房的卵巢良性黏液性囊腺瘤和交界性黏液性囊腺瘤的瘤壁及间隔厚度;对比MRI诊断卵巢良性黏液性囊腺瘤和交界性黏液性囊腺瘤的准确度;分析MRI对卵巢交界性黏液性囊腺瘤的诊断价值。结果与良性组比较,交界组蜂窝状子房(20.00%vs. 45.24%)、囊壁及分隔不规则增厚≥5 mm(10.00%vs. 35.71%)、结节或乳头状突起≥5 mm(8.00%vs. 69.05%)、T1WI含高、等信号(18.00%vs. 59.52%)、T2WI含低、等信号(18.00%vs. 45.24%)出现率显著更高,T1WI单一低信号(72.00%vs. 19.05%)、T2WI单一高信号(70.00%vs. 45.24%)出现率更低(均P<0.05)。与良性组比较,交界组多房囊腺瘤间隔厚度[(3.14±1.03)mm vs.(6.23±1.15)mm]显著更高(t=13.590,P<0.05)。MRI对卵巢交界性黏液性囊腺瘤的检出正确率为90.48%,显著高于卵巢良性黏液性囊腺瘤检出正确率(72.00%),差异有统计学意义(χ^(2)=4.951,P<0.05)。MRI诊断卵巢交界性黏液性囊腺瘤的曲线下面积(AUC)为0.812。结论 MRI可以详细显示卵巢交界性黏液性囊腺瘤患者的病理特征,有效鉴别卵巢良性黏液性囊腺瘤和交界性黏液性囊腺瘤,具有一定临床应用价值。

关键词：卵巢黏液性囊腺瘤卵巢交界性黏液性囊腺瘤磁共振成像影像学特征诊断价值

Inhibitory gamma-aminobutyric acidergic neurons in the anterior cingulate cortex participate in the comorbidity of pain and emotion

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Neural Regeneration Research 2025年第10期20卷 2838-2854页

作者： Lu Guan Mengting Qiu Na Li Zhengxiang Zhou Ru Ye Liyan Zhong Yashuang xu Junhui Ren yi Liang Xiaomei Shao Jianqiao Fang Junfan Fang Junying Du Department of Neurobiology and Acupuncture Research Third School of Clinical MedicineZhejiang Chinese Medical UniversityHangzhouZhejiang ProvinceChina Key Laboratory for Research of Acupuncture Treatment and Transformation of Emotional Diseases Third School of Clinical MedicineZhejiang Chinese Medical UniversityHangzhouZhejiang ProvinceChina Fuchun Community Health Service Center of Fuyang District HangzhouZhejiang ProvinceChina

Pain is often comorbid with emotional disorders such as anxiety and *** of the anterior cingulate cortex has been implicated in pain and pain-related negative emotions that arise from impairments in inhibitory gamma-aminobutyric acid *** review primarily aims to outline the main circuitry(including the input and output connectivity)of the anterior cingulate cortex and classification and functions of different gamma-aminobutyric acidergic neurons;it also describes the neurotransmitters/neuromodulators affecting these neurons,their intercommunication with other neurons,and their importance in mental comorbidities associated with chronic pain *** understanding on their role in pain-related mental comorbidities may facilitate the development of more effective treatments for these ***,the mechanisms that regulate gamma-aminobutyric acidergic systems remain *** is also unclear as to whether the mechanisms are presynaptic or *** exploration of the complexities of this system may reveal new pathways for research and drug development.

关键词： anterior cingulate cortex anxiety chronic pain circuit communication comorbidity depression gamma-aminobutyric acidergic neurons parvalbumin neurons synaptic transmission

Label distribution for multimodal machine learning

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Frontiers of Computer Science 2022年第1期16卷 33-43页

作者： yi REN ning xu Miaogen LING Xin GENG Department of Computer Science and Engineering Southeast UniversityNanjing211189China

Multimodal machine learning(MML)aims to understand the world from multiple related *** has attracted much attention as multimodal data has become increasingly available in real-world *** is shown that MML can perform better than single-modal machine learning,since multi-modalities containing more information which could complement each ***,it is a key challenge to fuse the multi-modalities in *** from previous work,we further consider the side-information,which reflects the situation and influences the fusion of *** recover multimodal label distribution(MLD)by leveraging the side-information,representing the degree to which each modality contributes to describing the ***,a novel framework named multimodal label distribution learning(MLDL)is proposed to recover the MLD,and fuse the multimodalities with its guidance to learn an in-depth understanding of the jointly feature ***,two versions of MLDL are proposed to deal with the sequential *** on multimodal sentiment analysis and disease prediction show that the proposed approaches perform favorably against state-of-the-art methods.

关键词： multimodal machine learning label distribution learning sentiment analysis disease prediction

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精准扶贫项目满意度影响因素分析

现代农业科技 2019年第3期 239-240页

作者：徐艺宁刘德钦王昌海西南林业大学经济管理学院云南昆明650224 中国社会科学院农村发展研究所

政府内部的考核,特别是被帮扶主体的反馈和独立第三方的评价对精准扶贫项目效果评估意义重大。在评价基础上对项目进行改进,这要求对精准扶贫项目评价的主要影响因素进行分析。本文采用有序probit模型,从贫困户和非贫困户角度对精准扶... 详细信息

政府内部的考核,特别是被帮扶主体的反馈和独立第三方的评价对精准扶贫项目效果评估意义重大。在评价基础上对项目进行改进,这要求对精准扶贫项目评价的主要影响因素进行分析。本文采用有序probit模型,从贫困户和非贫困户角度对精准扶贫项目满意度影响因素进行分析。研究结果表明,被访者特征、住房状况、扶贫评价显著影响贫困户和非贫困户对扶贫项目的满意度,民主参与指标不显著影响贫困户和非贫困户对扶贫项目的满意度。最后,对影响扶贫项目满意度的因素进行了总结,并提出了扶贫项目满意度影响因素研究的未来关注重点。

关键词：精准扶贫有序probit模型项目满意度影响因素

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某重型商用车整车电磁兼容测试研究

汽车电器 2022年第5期 69-73页

作者：赵雷雷朱亚萍罗旭东徐伊宁德创未来汽车科技有限公司陕西西安713700 比亚迪汽车有限公司陕西西安710119

本文以某重型商用车整车电磁兼容测试以及整改的实践为例,介绍整车电磁兼容测试、整改的方法,为整车电磁兼容测试提供参考。

关键词：电磁兼容重型商用车测试整改

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