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Genomics-assisted breeding——A revolutionary strategy for crop improvement

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Journal of Integrative Agriculture 2017年第12期16卷 2674-2685页

作者： LENG Peng-fei Thomas Lübberstedt xu ming-liang National Maize Improvement Center of China China Agricultural University Department of Agronomy Iowa State University

Food shortages arise more frequently owing to unpredictable crop yield losses caused by biotic and abiotic stresses. With advances in molecular biology and marker technology, a new era of molecular breeding has emerged that has greatly accelerated the pace of plant breeding. High-throughput genotyping technology and phenotyping platforms have enabled large-scale marker-trait association analysis, such as genome-wide association studies, to precisely dissect the genetic architecture of plant traits. Large-scale mapping of agronomically important quantitative trait loci, gene cloning and characterization, mining of elite alleles/haplotypes, exploitation of natural variations, and genomic selection have paved the way towards genomics-assisted breeding（GAB）. With the availability of more and more informative genomic datasets, GAB would become a promising technique to expedite the breeding cycle for crop improvement.

关键词： germplasm genotyping technology phenotyping platform genomics-assisted breeding

Identification and characterization of the GH3 gene family in maize

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Journal of Integrative Agriculture 2016年第2期15卷 249-261页

作者： ZHANG Dong-feng ZHANG Nan ZHONG Tao WANG Chao xu ming-liang YE Jian-rong National Maize Improvement Center/Beijing Key Laboratory of Crop Genetic Improvement China Agricultural University

The phytohormone auxin plays a central role in coordinating plant growth and ***3 is one of the three gene families that respond rapidly during auxin ***,we report the identification and characterization of the GH3 gene family in maize.A total of 12 GH3 genes were identified,which are not evenly distributed over the 10 maize *** GH3 protein sequences share a conserved domain which occupies nearly the entire *** c/＇s-elements were found in promoters of maize GH3 *** this study,the 12 maize GH3 proteins were primarily classified into two phylogenetic groups,similar to the 13 rice GH3 proteins,while 9 of the 19 Arabidopsis GH3 proteins were observed in the third phylogenetic *** analysis showed that expression of maize GH3 genes is temporally and spatially ***,maize GH3 genes displayed variable changes at transcript level upon pathogen *** presented here provide insight into the diversification and evolution of GH3 proteins,and lay a foundation for the functional characterization of these GH3 genes in future,especially for elucidating the mechanisms of GH3-mediated pathogenesis.

关键词： auxin GH3 family pathogen infection maize(Zea mays L.)

结合模糊(C+P)均值聚类和SP-V-支持向量机的TSK分类器

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光学精密工程 2016年第3期24卷 643-650页

作者：徐明亮王士同江南大学数字媒体学院江苏无锡214122 无锡环境科学与工程研究中心江苏无锡214063

为获得具有模糊规则自适应约简性能和较好的泛化性能的TSK分类器,本文提出了一种结合模糊(C+P)均值聚类(FCPM)算法和SP-V-支持向量机(SVM)分类算法来构建TSK(Takagi-Sugeno-Kang)分类器的方法。该方法首先用FCPM聚类算法对训练数据进行... 详细信息

为获得具有模糊规则自适应约简性能和较好的泛化性能的TSK分类器,本文提出了一种结合模糊(C+P)均值聚类(FCPM)算法和SP-V-支持向量机(SVM)分类算法来构建TSK(Takagi-Sugeno-Kang)分类器的方法。该方法首先用FCPM聚类算法对训练数据进行聚类;然后根据聚类结果确定TSK分类器的模糊规则前件中的高斯隶属度函数的中心和宽度参数;最后采用成组稀疏约束SP-V-SVM算法对模糊规则后件参数进行学习,该算法不仅改善了系统的泛化性能,还使系统具有模糊规则自适应约简功能,使得系统更为紧凑。与相关算法在UCI和IDA标准数据集分类实验中的模糊规则数和分类性能对比表明:用提出的分类算法所构造的TSK分类器不仅具有较好的分类性能,而且模糊规则数少,有利于构建更为紧凑的模糊分类系统。

关键词： TSK分类器模糊规则规则约简模糊(C+P)均值聚类(FCPM) SP-V-支持向量机(SVM)

Application of satellite altimetry technique in marine gravity surveying

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中国有色金属学会会刊：英文版 2005年第S1期15卷 92-95页

作者： xu Wei-ming, liang Kai-long, liang De-qing, ZHU Mu-hua, ZHANG Li-hua (Dalian Naval Academy, Dalian 116018, China)

Satellite altimetry can provide the geoid in ocean areas with a level of precision of about 10 cm. Such a precise geoid offers a good opportunity for studying the marine gravity field. The basic mathematical model and theory of the satellite altimetry were introduced briefly and firstly. Then the inverse Hotine method to derive gravity disturbances from satellite altimetry was presented. Meanwhile the inversion of marine gravity in Chinese coastal waters from Topex/Poseidon satellite altimetry data was tested, and the RMS between the gravity disturbance from the OSU91A model and satellite altimetry data inversing is about 12.76 mGal. Finally, some problems which are in inversing procedure were discussed, i e, integration singularity, edge effect etc. And it is conluded that the accuracy of the inversing gravity method can reach only a few mGal if proper treatment of Fast Fourier Transform (FFT) edge effect is employed.

关键词： satellite altimetry data inversion gravity disturbance

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植物免疫反应中的小RNA

遗传 2012年第1期34卷 41-49页

作者：徐凌徐明良中国农业大学国家玉米改良中心农业部作物基因组学与遗传改良重点开放实验室北京100193

植物中的小RNA参与多种生物学过程，依据其起源及前体结构的不同主要分为两类：微小RNA（miRNAs）和小干扰RNA（siRNAs），它们的长度通常为21～24个核苷酸，在生物合成途径以及作用机制等方面存在差异。病原物侵染植物后常通过诱导或... 详细信息

植物中的小RNA参与多种生物学过程，依据其起源及前体结构的不同主要分为两类：微小RNA（miRNAs）和小干扰RNA（siRNAs），它们的长度通常为21～24个核苷酸，在生物合成途径以及作用机制等方面存在差异。病原物侵染植物后常通过诱导或抑制小RNA分子来调节抗病相关基因的表达，进而调控植物与病原物的互作反应。文章就小RNA的生物合成、作用途径及其在植物与病原物互作中的调控机制等方面进行了综述。

关键词：小RNA 合成代谢植物病原菌互作抗病毒

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自适应RBF网络Q学习控制

控制与决策 2010年第2期25卷 303-306页

作者：徐明亮须文波江南大学信息工程学院江苏无锡214122

利用RBF网络逼近连续空间的Q值函数,实现连续空间的Q学习.RBF网络输入为状态-动作对,输出为该状态-动作对的Q值.状态由系统的状态转移特性确定,动作由优化网络输出得到的贪婪动作与服从高斯分布的噪声干扰动作两部分叠加而成.利用RNA算... 详细信息

利用RBF网络逼近连续空间的Q值函数,实现连续空间的Q学习.RBF网络输入为状态-动作对,输出为该状态-动作对的Q值.状态由系统的状态转移特性确定,动作由优化网络输出得到的贪婪动作与服从高斯分布的噪声干扰动作两部分叠加而成.利用RNA算法和梯度下降法自适应调整网络的结构和参数.倒立摆平衡控制的实验结果验证了该方法的有效性.

关键词： RBF网络自组织 Q学习连续空间优化

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单离子束技术概述

强激光与粒子束 2007年第11期19卷 1913-1917页

作者：詹福如许明亮许永建余增亮中国科学院离子束生物工程实验室合肥230031

对单离子束的发展和应用作了介绍。结合我国首台单离子束装置CAS-LIBB,综合讨论了准直器限流型和静电透镜聚焦型两种典型单离子束的技术结构。限流型结构简单但定位精度有限,聚焦型条件苛刻但可获得亚微米束,是单离子束发展的趋势。评... 详细信息

对单离子束的发展和应用作了介绍。结合我国首台单离子束装置CAS-LIBB,综合讨论了准直器限流型和静电透镜聚焦型两种典型单离子束的技术结构。限流型结构简单但定位精度有限,聚焦型条件苛刻但可获得亚微米束,是单离子束发展的趋势。评估了前探测、全前置探测和后探测3种单离子束探测方式及其特点,研究了这3种探测方式对辐照离子的计数精度和单离子束品质产生的影响。对CAS-LIBB装置研制了光导型全前置探测器以提高计数精度和束流品质。最后设计了快速荧光在线检测技术方案。

关键词：单离子束束流探测在线检测束流品质

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基于独立时空特征空间的人体运动合成

计算机学报 2011年第3期34卷 464-472页

作者：刘更代徐明亮张明敏西安电子科技大学计算机学院西安710071 浙江大学CADS＆CG国家重点实验室杭州310058

如何通过指定约束条件的方式交互式地合成风格化人体运动是计算机动画研究领域的热点和难点,传统的数据驱动办法通常没有全面考虑运动的静态和动态特性.针对这一问题,文中提出人体运动的独立时空特征空间模型,利用一个可变形运动模型和... 详细信息

如何通过指定约束条件的方式交互式地合成风格化人体运动是计算机动画研究领域的热点和难点,传统的数据驱动办法通常没有全面考虑运动的静态和动态特性.针对这一问题,文中提出人体运动的独立时空特征空间模型,利用一个可变形运动模型和独立特征子空间分析算法提取运动在时空两个域上的特征,并将其封装起来,通过低维子空间进行描述.运动风格的编辑可利用低维运动混合和空时约束优化等方法来实现.该方法充分考虑了运动在时域上的特征,并为用户提供了可编辑接口.文中以各种风格的行走运动为例讨论了该方法的有效性,结果证明效果良好,可用于交互式运动风格编辑.

关键词：独立时空特征子空间可变形运动模型独立特征子空间分析人体运动风格主成分分析

Superhydrophobic and corrosion-resistant siloxane-modified MgAl-LDHs coatings on magnesium alloy prepared under mild conditions

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 450-463页

作者： Wenxi Zhang Zhangzelong Zhuo Dan xu liang Wu Zhihui Xie Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province College of Chemistry and Chemical EngineeringChina West Normal UniversityNanchong 637002China College of Materials Science and Engineering/National Engineering Research Center for Magnesium Alloys Chongqing UniversityChongqing 400044China

We have developed a superhydrophobic and corrosion-resistant LDH-W/PFDTMS composite coating on the surface of Mg *** composite comprised a tungstate-intercalated(LDH-W)underlayer that was grown at low temperature(relative to hydrothermal reaction conditions)under atmospheric pressure and an outer polysiloxane layer created from a solution containing perfluorodecyltrimethoxysilane(PFDTMS)using a simple immersion *** successful intercalation of tungstate into the LDH phase and the following formation of the polysiloxane layer were confirmed through X-ray diffraction(XRD),Fourier transform infrared(FTIR)spectroscopy,and X-ray photoelectron spectroscopy(XPS).The corrosion resistance of the LDH-W film,both before and after the PFDTMS modification,was evaluated using electrochemical impedance spectroscopy(EIS),Tafel curves,and immersion *** results showed that Mg coated with LDH-W/PFDTMS exhibited significantly enhanced corrosion protection compared to the unmodified LDHW film,with no apparent signs of corrosion after exposure to 3.5wt%NaCl solution for 15 ***,the LDH-W/PFDTMS coating demonstrated superior superhydrophobicity and self-cleaning properties against water and several common beverages,as confirmed by static contact angle and water-repellency *** results offer valuable insights into preparing superhydrophobic and corrosion-resistant LDH-based composite coatings on Mg alloy surfaces under relatively mild reaction conditions.

关键词： Mg alloy corrosion coating layered double hydroxide