检索结果-南通市图书馆

AECOPD患者细胞黏附分子变化及其临床意义的研究

在线全文

学校读者我要写书评

暂无评论

现代实用医学 2011年第5期23卷 501-502,506页

作者：楼黎明胡丹丹董浙清刘文兵徐慧连浙江中医药大学附属第三医院杭州310005

目的观察慢性阻塞性肺疾病急性加重期(AECOPD)患者血清E-选择素(E-selectin)和血管细胞黏附因子-1(VCAM-1)水平,以及银杏叶提取物(GBE)对其的影响。方法将60例AECOPD患者随机分为常规组和GBE组,各30例;并选择同期30例健康体检者作为对... 详细信息

目的观察慢性阻塞性肺疾病急性加重期(AECOPD)患者血清E-选择素(E-selectin)和血管细胞黏附因子-1(VCAM-1)水平,以及银杏叶提取物(GBE)对其的影响。方法将60例AECOPD患者随机分为常规组和GBE组,各30例;并选择同期30例健康体检者作为对照组。两组基础治疗相同,GBE组每日加用舒血宁注射液20 ml静脉滴注,连用2周。应用酶联免疫吸附法检测血清E-selectin和VCAM-1的水平。两组均于入院第1天和治疗2周后行肺功能检查。结果治疗前常规组和GBE组E-selectin和VCAM-1水平均较对照组高(均<0.01);治疗后,两组E-selectin和VCAM-1水平均较治疗前显著降低(均<0.05),而GBE组较常规组下降更为显著(均<0.05)。两组1 s用力呼气量预测值、1 s用力呼气量/用力肺活量均较治疗前高,GBE组较治疗前差异有统计学意义(均<0.05),而常规组治疗前后无显著差异。结论银杏叶提取物可明显降低AECOPD患者血清E-selectin和VCAM-1水平,改善肺功能。

关键词：肺疾病,慢性阻塞性银杏叶提取物 E-选择素血管细胞黏附因子-1

EGFR siRNA序列的筛选及其对HepG2细胞活性的影响

在线全文

学校读者我要写书评

暂无评论

分子诊断与治疗杂志 2024年第5期16卷 935-939,944页

作者：许楠杨旭东薛丽宁启兰王慧莲耿燕西安交通大学第二附属医院医学检验科陕西西安710004 西安交通大学医学部遗传与分子生物学系陕西西安710061

目的构建EGFR shRNA重组真核表达载体,并筛选抑制效果最好的EGFR shRNA序列。方法应用在线工具设计人EGFR siRNA序列,采用限制性内切酶BamHI和HindIII切割真核表达质粒pcDNA3.1(+),构建三种人EGFR的siRNA干扰序列载体(psilencer 4.1-CMV... 详细信息

目的构建EGFR shRNA重组真核表达载体,并筛选抑制效果最好的EGFR shRNA序列。方法应用在线工具设计人EGFR siRNA序列,采用限制性内切酶BamHI和HindIII切割真核表达质粒pcDNA3.1(+),构建三种人EGFR的siRNA干扰序列载体(psilencer 4.1-CMV neo-EGFR siRNA)。将重组质粒载体转化大肠杆菌DH5α感受态并筛选阳性克隆,通过DNA测序鉴定重组质粒。应用脂质体lipo2000将三种人EGFR siRNA干扰序列载体转染到HepG2细胞,通过荧光显微镜观察转染效率,实时定量PCR检测EGFR mRNA表达水平,MTT法检测细胞活性。结果Psilencer 4.1-CMV neo-EGFR siRNA重组质粒被成功克隆。EGFR shRNA-1、EGFR shRNA-2和EGFR shRNA-3敲低EGFR mRNA的效率分别是80%、60%和70%以上。shRNA-2和shRNA-3使细胞活性分别下降50%(P0.05)。结论重组psilencer 4.1-CMV neo-EGFR siRNA质粒可下调肝癌细胞株EGFR表达水平和细胞活性。EGFR shRNA-3较EGFR shRNA-1和shRNA-2对HepG2细胞的抑制作用更显著。

关键词：基因治疗肝癌 HepG2 EGFR 小干扰RNA

原发性胆汁性胆管炎不同证型与肝组织病理相关性研究

在线全文

学校读者我要写书评

暂无评论

河北中医 2024年第9期46卷 1488-1492页

作者：陈沁磊陆玮婷乔飞史会连张帆徐祥涛江苏省中医院感染科江苏南京210001

目的探讨原发性胆汁性胆管炎(PBC)不同证型与肝组织病理的相关性。方法将88例PBC患者进行辨证分型、实验室检查、肝组织活检,统计合并疾病情况,常规判定PBC患者炎症评分和肝纤维化分期。结果PBC患者的中医证型分布由高到低依次为肝肾阴... 详细信息

目的探讨原发性胆汁性胆管炎(PBC)不同证型与肝组织病理的相关性。方法将88例PBC患者进行辨证分型、实验室检查、肝组织活检,统计合并疾病情况,常规判定PBC患者炎症评分和肝纤维化分期。结果PBC患者的中医证型分布由高到低依次为肝肾阴虚证、肝郁脾虚证、湿热蕴结证、瘀血阻络证。统计结果显示,肝郁脾虚证、湿热蕴结证熊去氧胆酸(UDCA)应答率高于瘀血阻络证(P<0.05),肝肾阴虚证、瘀血阻络证肝硬化发生率高于肝郁脾虚证(P<0.05);瘀血阻络证血清碱性磷酸酶(ALP)、总胆红素(TBiL)、直接胆红素(DBiL)、总胆汁酸(TBA)水平高于肝郁脾虚证(P<0.05),肝肾阴虚证TBA水平高于肝郁脾虚证(P<0.05),湿热蕴结证血清总胆固醇(TC)水平高于肝郁脾虚证(P<0.05)。4种证型在合并高脂血症、干燥综合征(SS)上比较差异有统计学意义(P<0.05),其中湿热蕴结证PBC患者高脂血症发生率高于肝郁脾虚证(P<0.05),肝肾阴虚证PBC患者SS发生率高于湿热蕴结证(P<0.05)。4种证型在肝组织炎症分级及纤维化分级分布上有显著差异(P<0.01)。其中,肝肾阴虚证、瘀血阻络证的纤维化分级显著高于肝郁脾虚证(P<0.01)。结论PBC肝肾阴虚证、瘀血阻络证可能与肝纤维化的程度相关。

关键词：胆管炎中医证型肝组织病理

中医肺康复模式在慢性阻塞性肺疾病稳定期的应用效果

在线全文

学校读者我要写书评

暂无评论

中国医药导报 2018年第10期15卷 58-62页

作者：田园李国勤徐慧莲李春雨屈建峰刘玉山楚晨李慧北京市东城区第一人民医院呼吸科北京100075 中国中医科学院广安门医院呼吸科北京100053

目的观察加用中医肺康复疗法与单纯药物治疗对慢性阻塞性肺疾病(COPD)稳定期患者的影响。方法选择于2015年11月~2016年11月北京市东城区第一人民医院就诊的COPD稳定期患者94例,采用随机数字表进行分组,康复组47例,对照组47例。对照组单... 详细信息

目的观察加用中医肺康复疗法与单纯药物治疗对慢性阻塞性肺疾病(COPD)稳定期患者的影响。方法选择于2015年11月~2016年11月北京市东城区第一人民医院就诊的COPD稳定期患者94例,采用随机数字表进行分组,康复组47例,对照组47例。对照组单纯药物治疗,康复组在药物治疗基础上联合中医肺康复疗法,统计治疗前后1年两组患者急诊入院次数及住院时间;分别于治疗前、治疗后1个月、治疗后3个月、治疗后6个月对COPD患者进行数据采集。检测指标包括圣乔治呼吸问卷(SGRQ);1秒钟用力呼气容积(FEV1)、1秒钟用力呼气容积占预计值百分比(FEV1%pred)、用力肺活量(FVC)、1秒钟用力呼气容积占用力肺活量的比值(FEV1/FVC)、每分钟最大通气量(MVV);6分钟步行试验及BODE指数评分。并对治疗效果进行分析评价。结果两组患者治疗后住院次数均低于治疗前,且治疗后康复组年住院次数均低于对照组,差异有统计意义(P<0.05);康复组治疗后SGRQ评分均低于同期对照组(P<0.05);康复组治疗后1个月MVV优于对照组(P<0.05);治疗后3个月FEV1%pred、FVC、MVV优于对照组(P<0.05);治疗后6个月FEV1、FEV1%pred、FVC、MVV、FEV1/FVC均优于对照组(P<0.05);康复组治疗后6分钟步行试验结果均优于对照组(P<0.05);康复组治疗后BODE指数评分均低于对照组(P<0.05)。结论中医肺康复模式能够提升COPD患者的日常生活能力和生存质量,提高活动耐力和呼吸肌功能,从而改善药物难以逆转的肺功能。

关键词：慢性阻塞性肺疾病中医肺康复日常生活能力生存质量活动耐力呼吸肌功能肺功能

Identification of major compounds of total flavonoids from Smilax china and evaluation of anti-inflammatory effect on phenol mucilage-induced pelvic inflammation in rats

在线全文

学校读者我要写书评

暂无评论

Journal of Chinese Pharmaceutical Sciences 2021年第2期30卷 157-168页

作者： Jiaqian Chen Chen Jin Bolun xu Huang Zhan Rongrong Fu Fengqin Li huilian Huang Key Laboratory of Modern Preparation of Traditional Chinese Medicine Jiangxi University of Traditional Chinese MedicineNanchang 330004China

As a Chinese medicinal plant,Smilax china is traditionally used in the treatment of pelvic inflammatory disease(PID).Flavonoids have been identified as the anti-inflammatory bioactive ***,the high content of total flavonoids has not been extracted from *** therapeutic effects and mechanisms of total flavonoids are still *** the present study,we aimed to obtain the effective parts of flavonoids in ***,and elucidate the mechanism of action on *** anti-inflammatory effect was evaluated on phenol mucilage-induced pelvic inflammation in *** was the first time that total flavonoids in high concentration(up to 55.6%)were obtained in ***.A total of 15 compounds were separated and identified by NMR,and the total flavonoids were detected with *** caused a reduction in serum levels of IL-6 and IL-1β.These results suggested that TFSC had a significant anti-PID effect,probably due to inhibition of the inflammatory reaction.

关键词： Smilax china Total flavonoids NMR Pelvic inflammation disease

Leaf Turgor Potential, Plant Growth and Photosynthesis in Organically Fertilized Sweet Corn

在线全文

学校读者我要写书评

暂无评论

Pedosphere 2004年第2期14卷 165-170页

作者： xuHui-Liant WANGXiao-Ju WANGJi-Hua xuRong-Ya ZHAOAi-Hua InternationalNatureFarmingResearchCenter HataNagano390-1401（Japan）.E-mail：huilian@janis.or.jp SaitamaCenterofEnvironmentalSciences Saitama347-0115（Japan） BeijingAcademyofAgricultureandForestrySciences Beijing100081（China）

A greenhouse pot experiment was conducted using a complete random design with six replications. A pressure-volume curve analysis was employed to study the effects of organic fertilizers on plant water relations in sweet corn (Zea mays L. cv. Honey-bantam) in terms of leaf osmotic concentration (Cs), osmotic potentials at full turgid status (πFT) and at incipient plasmolysis (πIP), and of symplastic (ζsym) and apoplastic (ζapo) fractions in the tissue water compartment in relation to photosynthetic capacity (Pc) and dry mass accumulation. At the seedling stage (day 15), plants with chemical fertilizer treatments showed lower πFT, πIP and ζapo and higher Cs, ζsym and PC than those with organic fertilizer treatments. Compared to PC and relative growth, where values from day 15 to day 75 were first lower for organic treatments and then higher, ζsym and Cs values were similar, while πFT and πIP were opposite being higher then lower. Dry mass production with organic fertilizer was higher than or close to the chemical fertilizer treatments in the later growth stage (day 75), though dry mass production with chemical fertilizers was much higher in the early and middle growing stages (days 15 and 45). Increased photosynthesis and dry mass production in later growth stages due to organic fertilizers were associated with increased osmotic concentration in the tissue and the symplastic fraction of the tissue water compartment. These might favor stomatal opening and biochemical activities.

关键词： leaf photosynthesis osmotic potential sweet corn (Zea mays L.) turgor potential water potential

Prediction value of high-grade prostatic intraepithelial neoplasia for prostate cancer on repeat biopsies

在线全文

学校读者我要写书评

暂无评论

The Chinese-German Journal of Clinical Oncology 2011年第7期10卷 410-414页

作者： huilian Hou xu Li Xingfa Chen Chunbao Wang Guanjun Zhang Honghan Wang Huilin Gong Yuan Deng Min Wang xuebin Zhang Department of Pathology The Fist Affiliated Hospital of Medical School of Xi＇an Jiaotong University Xi＇an 710061 China Experimental Medicine Center The Fist Affiliated Hospital of Medical School of Xi＇an Jiaotong University Xi＇an 710061 China Department of Urology The Fist Affiliated Hospital of Medical School of Xi＇an Jiaotong University Xi＇an 710061 China

Objective: The significance of isolated high-grade prostatic intraepithelial neoplasia in initial biopsy as an predic-tor for prostate cancer has been extensively research, and the true relationship remnant is no clear till now. The aim of this study is to evaluate prediction value of cancer on repeat biopsy in patients with high-grade prostatic intraepithelial neoplasia, using multivariate analysis. Methods: Thirty-eight men with a diagnosis of isolated high-grade prostatic intraepithelial neo-plasia in initial needle biopsy were studies, in the Fist Affiliated Hospital of Medical School of Xi'an Jiaotong University, from January 2003 to March 2009. These samples were using immunostaining of p63 and 34βE12 and P504s, with a median fol-low-up of 525 (range, 7 to 1650) days, and to researched the incidence of subsequent prostate cancer, and to predicted the risk of prostate cancer in clinicopathological parameters of isolated high-grade prostatic intraepithelial neoplasia on repeat biopsies by logistic regression analysis. Results: There were 10 of 38 (26.3%) men with prostate cancer on repeat biopsies after diagnosis isolated high-grade prostatic intraepithelial neoplasia in initial biopsy, of the rates of prostate cancer were 80% for micropapillary and 75% for cribriform high-grade prostatic intraepithelial neoplasia (P < 0.05), respectively. The positive cores of isolated high-grade prostatic intraepithelial neoplasia was the important for the risk of prostate cancer using Multi-factor logistic regression analysis. The time range in 30 to 690 days was stronger risk for prostate cancer detection after diagnosis isolated HGPIN in initial biopsy. p63 and 34βE12 were disrupted positive expression, and P504S was weak posi-tive expression in the 61% isolated high-grade prostatic intraepithelial neoplasia. Conclusion: Isolated high-grade prostatic intraepithelial neoplasia on repeat biopsy conferred a 26.3% risk of prostate cancer, and this risk level is lower than the p

关键词： high-grade prostatic intraepithelial neoplasia (HGPIN) prostate cancer (PCa) repeat biopsy prediction

在线全文

学校读者我要写书评

暂无评论

赣粤地区蕨类植物区系新资料

热带作物学报 2022年第9期43卷 1788-1796页

作者：徐国良赖辉莲张昌友江西九连山国家级自然保护区管理局江西赣州341700

2021年1—12月期间,通过对南岭山地东部赣粤边境九连山地区的植物调查,新增4种江西省蕨类分布新记录,即针毛新月蕨(Pronephrium hirsutum Ching ex ***)、蓝色鳞毛蕨(Dryopteris polita Rosenst.)、车前蕨(Antrophyum henryi Hieron.)、团羽鳞盖蕨(Microlepia obtusiloba Hayata)。新增5种广东省蕨类植物新记录,即无盖耳蕨(Polystichum gymnocarpium Ching ex ***&***)、东京鳞毛蕨[Dryopteris tokyoensis(*** Makino)***.]、半岛鳞毛蕨(Dryopteris peninsulae Kitag.)、尾叶稀子蕨[Monachosorum flagellare(*** Makino)Hayata]、亮毛蕨[Acystopteris japonica(Luerss.)Nakai]。新增1个中国大陆蕨类新记录种,即台湾金星蕨[Parathelypteris castanea(Tagawa)Ching]。新增1个广东省蕨类新分布科,即冷蕨科(Cystopteridaceae Schmakov),新增1个广东省新记录属,即亮毛蕨属(Acystopteris Nakai),其中东京鳞毛蕨和车前蕨分别被《IUCN濒危物种红色名录》收录为濒危种(EN)和易危种(VU),新记录的凭证标本存放于九连山国家级自然保护区植物标本馆(JNR)内,本文分别描述它们的形态特征、采集地点、生长环境、分布地及与近缘种的主要形态区别;讨论冷蕨科和亮毛蕨属在广东省内被发现的地理区系意义,中国台湾金星蕨、团羽鳞盖蕨、针毛新月蕨、半岛鳞毛蕨、蓝色鳞毛蕨、无盖耳蕨、尾叶稀子蕨、针毛新月蕨、东京鳞毛蕨等新记录被发现的地理区系意义;提出分布于中国热带和南亚热带地区的蓝色鳞毛蕨在江西省被发现,更新了其在中国分布的最高纬度记录;提出新记录的发现可为研究该类物种的起源、扩散机制、气候和环境的变化提供参考;提出中国特有种无盖耳蕨同时也为华南地区的蕨类新记录;提出上述新记录的发现进一步丰富了中国大陆、江西省和广东省的蕨类生物多样性,建议当地对各种蕨类新记录,尢其是珍稀濒危蕨类新记录的生境状况、种群和群落特征进一步调查并进行动态监测,必要时采取适当的保护管理措施。

关键词：新记录种新记录属新记录科江西省广东省

维普期刊数据库博看期刊

量子点的荧光共振能量转移在生物分析中的应用

在线全文

学校读者我要写书评

暂无评论

分析化学 2005年第9期33卷 1335-1338页

作者：马慧莲刘含智王丽萍徐淑坤李惟东北大学理学院吉林大学生命科学学院吉林大学生命科学学院沈阳110004 沈阳110004 长春130023

量子点良好的光谱特性和自身优点,使其可以作为生物荧光探针,而替代传统荧光染料。近年来这方面的研究已经取得了一定的进展。目前,量子点在共振能量转移方面的研究,进一步扩展了它在生物分析中的应用。介绍了量子点基于共振能量转移原... 详细信息

量子点良好的光谱特性和自身优点,使其可以作为生物荧光探针,而替代传统荧光染料。近年来这方面的研究已经取得了一定的进展。目前,量子点在共振能量转移方面的研究,进一步扩展了它在生物分析中的应用。介绍了量子点基于共振能量转移原理在生物分析中的应用,即利用量子点设计的两种类的蛋白-蛋白特异性结合分析和3种生物传感器的模型设计。

关键词：量子点荧光共振能量转移生物荧光探针生物分析评述