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不同灌溉模式对籼粳杂交稻甬优1540产量与水分利用效率的影响

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作物学报 2024年第2期50卷 425-439页

作者：徐冉杨文叶朱均林陈松徐春梅刘元辉章秀福王丹英褚光中国水稻研究所/水稻生物育种全国重点实验室浙江杭州 311400 襄阳市农业科学院湖北襄阳 441057 杭州市农业技术推广中心浙江杭州 310000 中国水稻研究所/水稻生物育种全国重点实验室浙江杭州 311400

旨在探讨不同灌溉模式对籼粳杂交稻甬优1540产量与水分利用效率的影响,并阐明其相关生理基础.本研究以甬优1540为材料,设置了 3种灌溉模式,即长淹灌溉(continuous flooding,CF)、轻度干湿交替灌溉(alternate wetting and moderate dryin... 详细信息

旨在探讨不同灌溉模式对籼粳杂交稻甬优1540产量与水分利用效率的影响,并阐明其相关生理基础.本研究以甬优1540为材料,设置了 3种灌溉模式,即长淹灌溉(continuous flooding,CF)、轻度干湿交替灌溉(alternate wetting and moderate drying,AWMD)以及重度干湿交替灌溉(alternate wetting and severe drying,AWSD).研究结果表明,与CF相比,AWMD与AWSD均能显著提高水分利用效率,增幅分别为22.6％～25.6％与18.2％～23.1％;AWMD可以显著提高水稻产量,增幅为8.6％～10.0％,而AWSD则显著降低水稻产量,降幅为6.0％～7.5％.与CF相比,AWMD显著降低了拔节期水稻的茎蘖数、地上部干物质重、叶面积指数、移栽至齐穗期的光合势以及移栽至拔节期的作物生长速率,但显著提高了茎蘖成穗率、拔节至齐穗期的作物生长速率、主要生育期水稻根长密度、深根比、比根长、根系总吸收表面积与活跃吸收表面积,以及灌浆后2次土壤复水期的剑叶净光合速率、根系氧化力、根系与叶片中玉米素和玉米素核苷(Z+ZR)含量、籽粒中蔗糖-淀粉代谢途径关键酶活性等指标.以上结果表明,AWMD可以协同提高甬优1540产量与水分利用效率,优化根-冠生长发育特征,提高灌浆期植株生理活性,实现高产与水分高效利用,为本研究最佳水分管理模式.

关键词：籼粳杂交稻干湿交替灌溉产量水分利用率根系形态与生理特征

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脂肪干细胞在牙周组织再生中应用研究进展

中国实用口腔科杂志 2024年第3期17卷 365-371页

作者：徐名颉王锦衡谢旭东许春梅王骏口腔疾病防治全国重点实验室国家口腔医学中心国家口腔疾病临床医学研究中心四川大学华西口腔医院牙周病科四川成都610041

牙周炎是一种常见的口腔炎性疾病,可导致牙周支持组织(牙龈、牙槽骨、牙周膜和牙骨质)丧失,甚至造成失牙,严重影响患者的生活质量。实现牙周组织再生进而恢复正常的牙周组织生理结构和功能是牙周治疗的终极目标,而基于干细胞的组织再生... 详细信息

牙周炎是一种常见的口腔炎性疾病,可导致牙周支持组织(牙龈、牙槽骨、牙周膜和牙骨质)丧失,甚至造成失牙,严重影响患者的生活质量。实现牙周组织再生进而恢复正常的牙周组织生理结构和功能是牙周治疗的终极目标,而基于干细胞的组织再生工程技术是目前实现牙周组织再生的重要技术手段。脂肪干细胞(adipose-derived stem cells,ADSCs)是一种来源于脂肪组织的间充质干细胞,具有自我复制和多向分化潜能;其可参与组织再生、血管重建及免疫调节过程,是牙周组织再生的理想种子细胞之一。文章就近年来ADSCs在牙周组织再生中的应用研究进展做一综述,以期为牙周组织再生治疗提供研究方向。

关键词：脂肪干细胞间充质干细胞牙周组织再生骨再生免疫调节

Photosynthesis and Dry Matter Accumulation in Different Chlorophyll-Deficient Rice Lines

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Journal of Integrative Agriculture 2012年第3期11卷 397-404页

作者： WANG Dan-ying xu chun-mei CHEN Song TIAO Long-xing ZHANG Xiu-fu China National Rice Research Institute Hangzhou 310006P.R.China

Three different chlorophyll-deficient rice isogenic lines chl,fgl and pgl,and their recurrent parent zhefu802 （zf802） were used to study effects of leaf color on photosynthesis,dry matter accumulation,yield,and quality in early season indica *** showed that the chlorophyll （Chl.） a/b ratio of isogenic lines chl-8,pgl and fgl was 5.35,10.00 and 15.46,respectively,among them,line fgl had higher leaf area index （LAI）,higher net photosynthetic rate and higher grain-filling rate than its recurrent parent zf802 at the later period of grain filling stage;while LAI,net photosynthetic rate and dry matter accumulated in lines chl-8 and pgl were lower than in *** were found in the grain yield and quality among chlorophyll deficient isogenic lines,lines fgl,chl-8 and zf802 had similar grain yield,which was significantly higher than that of pgl;the highest milling quality was observed in isogenic line fgl,with relatively high protein *** study showed that isogenic line fgl would become a unique material for the development of high yield rice with high grain quality because of its slow aging process and relative steady grain-filling rate.

关键词： rice photosynthesis dry matter accumulation chlorophyll-deficient isogenic lines

Geographic variation in the yield formation of single-season high-yielding hybrid rice in southern China

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Journal of Integrative Agriculture 2021年第2期20卷 438-449页

作者： WANG Dan-ying LI xu-yi YE Chang xu chun-mei CHEN Song CHU Guang ZHANG Yun-bo ZHANG Xiu-fu State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 310006P.R.China Crop Research Institute Sichuan Academy of Agricultural SciencesChengdu 610066P.R.China Hubei Collaborative Innovation Center for Grain Industry Yangtze UniversityJingzhou 434025P.R.China

Environmental conditions greatly affect the growth of rice. To investigate the geographic differences in yield formation of single-season high-yielding hybrid rice in southern China, experiments were conducted in 2017 and 2018 in the upper and middle–lower reaches of the Yangtze River with 10–30 main locally planted high-yielding hybrid cultivars used as materials. Compared with rice planted in the middle–lower reaches of the Yangtze River, rice planted in the upper reaches has a longer tillering duration, higher accumulated temperature(≥10℃) during tillering period, but lower accumulated temperature and solar radiation from initial booting to maturity. Yield traits comparison between the upper and the middle–lower reaches of Yangtze River showed that the former had 48.1% more panicles per unit area while the latter had 46.4% more grains per panicle;the rice yield in the former was positively correlated with the seed setting rate and the dry matter accumulation before heading, while the latter was positively correlated with grains per panicle and dry matter accumulation from booting to maturity. Comparison of the same variety Tianyouhuazhan planted in different regions showed there was a significant positive correlation between panicle number and the duration of and accumulated temperature during the tillering period(r=0.982^(**), r=0.993^(**), respectively), and between grains per panicle and accumulated solar radiation during booting period(r=0.952~*). In the upper reaches of the Yangtze River, more than 90% of cultivars with an yield of greater than 11 t ha^(–1) had an effective panicle number of 250–340 m^(–2), and there was a significant negative correlation between seed setting rate and grains per panicle;therefore, the high-yielding rice production in these regions with a long effective tillering period(>40 d) should choose varieties with moderate grains per panicle, adopt crop managements such as good fertilizer and water measures during vegetative growth pe

关键词： single-season hybrid rice geographic variation yield climatic factor southern China

Experimental observations of ferroelectricity in double pyrochlore Dy2Ru2O7

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Frontiers of physics 2014年第1期9卷 82-89页

作者： Zai-chun xu mei-Feng Liu Lin Lin Huimei Liu Zhi-Bo Yan Jun-Ming Liu Laboratory of Solid State Microstructures Nanjing University Nanjing 210093 China

Double pyrochlore Dy2Ru207 is synthesized and its magnetism and ferroelectricity below the Ru4＋ spin ordering temperature （NI00 K） are investigated. The ferroelectric transition appears at -18 K, much higher than the Dy3＋ spin ordering point at -1.8 K and lower than the Ru4＋ spin ordering point at -100 K. The measured electric polarization at ,-2 K is as big as 145℃/m2 in the polycrystalline samples. It is argued that the ferroelectricity is possibly ascribed to the electric dipole ordering arising from the collective monopole excitations in the Dy3＋ tetrahedrons in prior to the Dy3＋ spin ordering into spin-ice like state below -1.8 K.

关键词： pyrochlore oxides multiferroics magnetically induced ferroelectricity

Changes in the activities of key enzymes and the abundance of functional genes involved in nitrogen transformation in rice rhizosphere soil under different aerated conditions

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Journal of Integrative Agriculture 2023年第3期22卷 923-934页

作者： xu chun-mei XIAO De-shun CHEN Song CHU Guang LIU Yuan-hui ZHANG Xiu-fu WANG Dan-ying The State Key Laboratoty of Rice Biology China National Rice Research InstituteHangzhou 310006P.R.China

Soil microorganisms play important roles in nitrogen transformation. The aim of this study was to characterize changes in the activity of nitrogen transformation enzymes and the abundance of nitrogen function genes in rhizosphere soil aerated using three different methods(continuous flooding(CF), continuous flooding and aeration(CFA), and alternate wetting and drying(AWD)). The abundances of amoA ammonia-oxidizing archaea(AOA) and ammonia-oxidizing bacteria(AOB), nirS, nirK, and nifH genes, and the activities of urease, protease, ammonia oxidase, nitrate reductase, and nitrite reductase were measured at the tillering(S1), heading(S2), and ripening(S3) stages. We analyzed the relationships of the aforementioned microbial activity indices, in addition to soil microbial biomass carbon(MBC) and soil microbial biomass nitrogen(MBN), with the concentration of soil nitrate and ammonium nitrogen. The abundance of nitrogen function genes and the activities of nitrogen invertase in rice rhizosphere soil were higher at S2 compared with S1 and S3 in all treatments. AWD and CFA increased the abundance of amoA and nifH genes, and the activities of urease, protease, and ammonia oxidase, and decreased the abundance of nirS and nirK genes and the activities of nitrate reductase and nitrite reductase, with the effect of AWD being particularly strong. During the entire growth period, the mean abundances of the AOA amoA, AOB amoA, and nifH genes were 2.9, 5.8, and 3.0 higher in the AWD treatment than in the CF treatment, respectively, and the activities of urease, protease, and ammonia oxidase were 1.1, 0.5, and 0.7 higher in the AWD treatment than in the CF treatment, respectively. The abundances of the nirS and nirK genes, and the activities of nitrate reductase and nitrite reductase were 73.6, 84.8, 10.3 and 36.5% lower in the AWD treatment than in the CF treatment, respectively. The abundances of the AOA amoA, AOB amoA, and nifH genes were significantly and positively correlated wi

关键词： rhizosphere aeration gene abundance enzyme activities soil microbial biomass carbon soil microbial nitrogen

3D-QSAR and Surflex Docking Studies of a Series of Alkaline Phosphatase Inhibitors

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Chinese Journal of Structural Chemistry 2019年第1期38卷 7-16,1页

作者： SHU Mao WU Tao WANG Bi-Wu LI Jing xu chun-mei LIN Zhi-Hua School of Pharmacy and Bioengineering Chongqing University of Technology

Alkaline phosphatases(APs) include the placental AP(PLAP), germ cell AP(GCAP), intestinal AP(IAP) and tissue nonspecific AP(TNAP). Over expression of TNAP in smooth muscle cells of kidney and vessels provokes the progress of such serious diseases as end-stage renal disease, idiopathic infantile arterial calcification, ankylosis, osteoarthritis and diabetes. In order to design and optimize the potent TNAP inhibitors, comparative molecular field analysis(CoMFA) and comparative molecular similarity indices analysis(CoMSIA) were used to analyze 3D structure-activity relationships(3D-QSAR) of TNAP inhibitors. The 3D-QSAR model(CoMFA with q^2 = 0.521, r^2 = 0.930; CoMSIA with q^2 = 0.529, r^2 = 0.933) had a good predictability. Surflex-dock was used to reveal the binding mode between the inhibitors and TNAP protein. CoMFA, CoMSIA and docking results provide guidance for the discovery of TNAP inhibitors. Finally, eight new compounds as potential TNAP inhibitors were designed.

关键词： 3D-QSAR surflex-dock alkaline phosphatase inhibitors

Effects of Aeration on Root Physiology and Nitrogen Metabolism in Rice

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Rice science 2013年第2期20卷 148-153页

作者： xu chun-mei WANG Dan-ying CHEN Song CHEN Li-ping ZHANG Xiu-fu State Key Laboratory of Rice Biology China National Rice Research Institute

In order to clarify the effects of aeration on root nitrogen metabolism in rice seedlings,rice cultivars Guodao 6 （indica） and Xiushui 09 （japonica） were investigated for root growth,the activities of glutamine synthetase （GS）,glutamic acid-pyruvic acid transaminase （GPT） and glutamic acid oxaloacetate transaminase （GOT）,the nitrate （NO 3-N） concertration,the contents of free amino acids and soluble sugar in root under hydroponics with continuous aeration *** results showed that rice seedlings grown in oxygenation solutions had higher root dry matter,longer root length,stronger root activity and larger root absorption area compared with the *** addition,the contents of soluble sugar,root vigor and the activities of GS,GOT and GPT in the aeration solutions were higher than those in the *** results also indicated that the activities of enzymes involved in root nitrogen metabolism of Xiushui 09 were enhanced by aeration,however,there was no significant influence on root nitrogen metabolism of Guodao 6,which suggested that effect of oxygenation on rice root nitrogen metabolism might be genotype-specific.

关键词： rice aeration root physiology nitrogen metabolism

MiRNA-145-5p inhibits gastric cancer progression via the serpin family E member 1-extracellular signal-regulated kinase-1/2 axis

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World Journal of Gastrointestinal Oncology 2024年第5期16卷 2123-2140页

作者： Hong-Xia Bai xue-mei Qiu chun-Hong xu Jian-Qiang Guo Department of Gastroenterology The Second Hospital of Shandong UniversityJinan 250000Shandong ProvinceChina Department of Gastroenterology Liaocheng People’s HospitalLiaocheng 252000Shandong ProvinceChina Department of Reproductive Center Zaozhuang Maternal and Child Health Care HospitalZaozhuang 277000Shandong ProvinceChina

BACKGROUND MicroRNAs(miRNAs)regulate gene expression and play a critical role in cancer ***,there is still a limited understanding of the function and regulatory mechanism of miRNAs in gastric cancer(GC).AIM To investigate the role and molecular mechanism of miRNA-145-5p(miR145-5p)in the progression of *** Real-time polymerase chain reaction(RT-PCR)was used to detect miRNA expression in human GC tissues and *** ability of cancer cells to migrate and invade was assessed using wound-healing and transwell assays,*** proliferation was measured using cell counting kit-8 and colony formation assays,and apoptosis was evaluated using flow *** of the epithelial-mesenchymal transition(EMT)-associated protein was determined by Western *** of miR-145-5p were predicated using bioinformatics analysis and verified using a dual-luciferase reporter *** family E member 1(SERPINE1)expression in GC tissues and cells was evaluated using RT-PCR and immunohistochemical *** correlation between SERPINE1 expression and overall patient survival was determined using Kaplan-meier plot *** association between SERPINE1 and GC progression was also tested.A rescue experiment of SERPINE1 overexpression was conducted to verify the relationship between this protein and *** mechanism by which miR-145-5p influences GC progression was further explored by assessing tumor formation in nude *** GC tissues and cells had reduced miR-145-5p expression and SERPINE1 was identified as a direct target of this *** of miR-145-5p was associated with decreased GC cell proliferation,invasion,migration,and EMT,and these effects were reversed by forcing SERPINE1 ***-meier plot analysis revealed that patients with higher SERPINE1 expression had a shorter survival rate than those with lower SERPINE1 *** mouse tumorigenesis experiments confirmed that miR-145-5p target

关键词： Gastric cancer MicroRNA-145-5p Serpin family E member 1 Epithelial-mesenchymal transition Proliferation Extracellular signal-regulated kinase-1/2