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CD8＋ Tc-lymphocytes immunodeviation in peripheral blood and airway from patients of chronic obstructive pulmonary disease and changes after short-term smoking cessation

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Chinese Medical Journal 2013年第19期 3608-3615页

作者： YU Mu-qing LIU Xian-sheng WANG jian-miao xu yong-jian Department of Respiratory and Critical Care Medicine TongjiHospital of Yongji Medical College Huazhong University of Scienceand Technology Wuhan Hubei 430030 China

Background Cigarette smoke induces an acute but persisting inflammation in peripheral blood and airway in chronic obstructive pulmonary disease （COPD）,and CD8＋ Tc-lymphocytes are considered as a key role in this *** aimed to investigate the Tc-lymphocytes immunodeviation in system and local airway of COPD patients and changes of the immunodeviation after short-term smoking *** Peripheral blood （PB） and bronchoalveolar lavage fluid （BALF） were collected from 42 patients （14 COPD patients,16 smokers with normal lung function and 12 nonsmokers）,while PB and induced sputum （IS） were obtained from other 19 patients （10 quitting smokers and 9 continuing smokers） at baseline and follow-up respectively of 4-week smoking *** of CD8＋ Tc-lymphocytes （%CD3＋） and Tc1/Tc2 ratios were measured by flow *** Percentages of CD8＋ Tc-lymphocytes were higher in COPD patients than those in smokers and nonsmokers in both PB and ***1/Tc2 ratio in PB and in BALF from COPD patients was greater than that from smokers and nonsmokers and negatively correlated with FEV1%*** comparing the ratios between PB and BALF,significantly positive correlation was found in COPD ***,after 4-week smoking cessation,percentages of CD8＋ Tc-lymphocytes in PB and IS in quitting smokers were decreased compared to that in baseline and continuing smokers,whereas Tc1/Tc2 ratios were not *** CD8＋ Tc1-trend immunodeviation profiles occurred in both system and local airway of COPD *** exceptional immunodeviation could not be relieved by short-term smoking cessation.

关键词： pulmonary disease, chronic obstructive smoke CD8-positive T-lymphocytes smoking cessation

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关于带钢冷轧机选型的探讨

钢铁 2008年第5期43卷 1-6页

作者：许健勇宝钢股份有限公司宝钢分公司冷轧厂上海200941

出于满足产品大纲中产品的强度等级、产品的宽度与厚度规格范围、机组的生产能力、板形调控能力及投资的合理性等因素的考虑,探讨了如何选择带钢冷轧机的机型及机组的配置。剖析了国内外20世纪80年代后建设的46套带钢冷连轧机的发展趋势。

关键词：生产规模轧制方式机型投资板形

Effect of cigarette smoke extract on lipopolysaccharide-activated mitogen-activated protein kinase signal transduction pathway in cultured cells

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Chinese Medical Journal 2007年第12期120卷 1075-1081页

作者： LI Wen xu yong-jian SHEN Hua-hao Department of Respiratory Medicine Second Affiliated Hospital College of Medicine and Institute of Respiratory Diseases Zhejiang University Hangzhou 310009 China Department of Respiratory Medicine Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

Background Lipopolysaccharide （LPS） forms outer membrane of the wall of Gram-negative cells. LPS can directly cause damage to epithelia of respiratory tract and is the major factor responsible for the chronic inflammation of respiratory passage. The mitogen-activated protein kinase （MAPK） signal transduction pathway of the airway epithelia is intimately associated with the action of LPS. The chronic inflammation of respiratory tract and smoking are interrelated and entwined in the development and progression of chronic lung diseases. This study was designed to examine the effects of cigarette smoke extract （CSE） and LPS on MAPK signal transduction pathway in order to further understand the roles CSE and LPS play in chronic lung inflammation. Methods Cultured primary human epithelial cells of airway were divided into four groups according to the stimulants used： blank control group, LPS-stimulation group, CSE-stimulation group and CSE plus LPS group. Western blotting was employed for the detection of phosphorylation level of extracellular-signal-regulated-kinase （ERK1/2）, p38 MAPK and c-Jun N-terminal kinase （JNK）. The expression of cytokines of MAPK transduction pathway （granulocyte-macrophage colony stimulating factor （GM-CSF） and mRNA of IL-8） in the primary epithelial cells of respiratory tract was also determined. Results Western blotting revealed that the phosphorylation levels of ERK1/2, p38 MAPK and JNK were low and 2 hours after the LPS stimulation, the phosphorylation of ERK1/2, p38 MAPK and JNK were all increased. There was a significant difference in the phosphorylation between the LPS-stimulation group and blank control group （P〈0.05）; no significant difference was found between CSE-stimulation group and blank control group （P〉0.05）; there was a significant difference between CSE ＋ LPS group and blank control group and between CSE ＋ LPS group and LPS group （P〈0.05）. The phosphorylation of CSE-LPS group was higher than that of blank control group but

关键词： smoking lipopolysaccharide mitogen-activated protein kinase signal transduction pathway interleukin-8 epithelial cells granulocyte-macrophage colony stimulating factor

Effect of Soothing Gan(Liver) and Invigorating Pi(Spleen) Recipes on TLR4-p38 MAPK Pathway in Kupffer Cells of Non-alcoholic Steatohepatitis Rats

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Chinese Journal of Integrative Medicine 2019年第3期25卷 216-224页

作者： GONG Xiang-wen xu yong-jian YANG Qin-he LIANG Yin-ji ZHANG Yu-pei WANG Guan-long LI Yuan-yuan School of Chinese Medicine Jinan University

Objective: To investigate the mechanism of inflammatory-mediated toll-like receptor 4(TLR4)-p38 mitogen-activated protein kinase(p38 MAPK) pathway in Kupffer cells(KCs) of non-alcoholic steatohepatitis(NASH) rats and the intervention effect of soothing Gan(Liver) and invigorating Pi(Spleen) recipes on this pathway. Methods: After 1 week of acclimatization, 120 Sprague-Dawley male rats were randomly divided into 8 groups using a random number table(n=15 per group): normal group, model group, low-dose Chaihu Shugan Powder(柴胡疏肝散, CHSG) group(3.2 g/kg), high-dose CHSG group(9.6 g/kg), low-dose Shenling Baizhu Powder(参苓白术散, SLBZ) group(10 g/kg), high-dose SLBZ(30 g/kg) group, and low-and highdose integrated recipe(L-IR, H-IR) groups. All rats in the model and treatment groups were fed with a high-fat diet(HFD). The treatments were administrated by gastrogavage once daily and lasted for 26 weeks. The liver tissues were detected with hematoxylin-eosin(HE) and oil red O staining. Levels of liver lipids, serum lipids and transaminases were measured. KCs were isolated from the livers of rats to evaluate the mRNA expressions of TLR4 and p38 MAPK by real-time fluorescence quantitative polymerase chain reaction, and proteins expressions of TLR4, p-p38 MAPK and p38 MAPK by Western blot. Levels of inflammatory cytokines including tumor necrosis factor α(TNF-α), interleukin(IL)-1 and IL-6 in KCs were measured by enzyme-linked immunosorbent assay. Results: After 26 weeks of HFD feeding, HE and oil red O staining showed that the NASH model rats successfully reproduced typical pathogenesis and histopathological features. Compared with the normal group, the model group exhibited significant increases in body weight, liver weight, liver index, serum levels of total cholesterol(TC), triglyceride(TG), low-density lipoprotein cholesterol, and aspartate aminotransferase as well as TC and TG levels in liver tissues, and significant decrease in serum level of high-density lipoprotein choleste

关键词： non-alcoholic steatohepatitis soothing Gan (Liver)and invigorating Pi (Spleen)recipes Kupffer cel toll-like receptor 4-p38 mitogen-activated protein kinase signaling pathway inflammation,Chinese medicine

Basic and clinical research into chronic obstructive pulmonary disease in China

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Chinese Medical Journal 2010年第4期123卷 491-497页

作者： LI Yuan-yuan LIU Xian-sheng xu yong-jian Department of Respiratory Medicine Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430030 China

Chronic obstructive pulmonary disease （COPD）, one of the most common public health problemsworldwide, is the fourth leading cause of death in the world. In China, respiratory diseases （of which COPD is a major component） are the third leading cause of death in rural areas and the fourth leading cause of death in urban area, accounting for 1 million deaths and over 5 million disabilities each year. COPD ranks first among the burdens in China.1 Hence, medical workers and governments at different levels pay close attention to this disease~ The Respiratory Group under the Chinese Medical Association published the Guidelines for Diagnosis and Management of COPD in 2002^2 and the revised version in 2007^3. This publication serves as a milestone of the development of basic and clinical investigation in China. The guidelines are likely to affect the way for the treatment of COPD in addition to reflect the current knowledge about the underlying causes of COPD and recommend the proper diagnosis and management of COPD.

关键词： pulmonary disease, chronic obstructive prevalence risk factors etiology therapy China

Preliminary Experimental Study of Ion Beam Extraction of EAST Neutral Beam Injector

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Chinese Physics Letters 2012年第3期29卷 139-141页

作者： xu yong-jian HU Chun-Dong LIU Sheng XIE Ya-Hong LIANG Li-Zhen jianG Cai-Chao Institute of Plasma Physics Chinese Academy of SciencesHefei 230031

Neutral beam injection is recognized as one of the most effective means for plasma *** preliminary data of ion beam extraction is obtained on the EAST neutral beam injector *** extraction from the ion source of EAST-NBI is verified by measuring the beam current with a Faraday cup and by analyzing the results obtained by means of water calorimetric measurement on the temperature rises of water cooling the accelerator electrodes.

关键词： beam injection verified

THE SPATIAL DIVISION OF INDUSTRIES OF THE PEARL RIVER DELTA IN THE 1990s: CHARACTERISTICS AND TRENDSS

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Chinese Geographical Science 2001年第1期11卷 63-69页

作者： xu yong-jian,YAN Xiao-pei,xu xue-qiang (Center for Urban and Regional Studies, Zhongshan University, Guangzhou 510275, P. R. China) Center for Urban and Regional Studies Zhongshan University Guangzhou P. R. China

The article analyzed the spatial division of industries of the Pearl River Delta (PRD) in the 1990s, a period that witnessed the deepening of the reform and opening policies and continued rapid development of the region. By adopting the index of specialization, the extent of specialization, its change and its spatial distribution in the region are measured and demonstrated. The research revealed that, despite the trend of more balanced development of the secondary sector between the PRD and the rest of the province, in the PRD, it is the unbalanced development that dominated and the spatial division of the secondary sector has been shaped. The tertiary industry has also been experiencing unbalance development and the spatial division of the tertiary sector has been emerging, its extent of concentration appearing even more evident than that of the secondary sector. The author also discussed the linkage between the spatial division of the two sectors. In the end of the paper, the trends of the spatial division of the industries in the PRD are forecasted.

关键词： Pearl River Delta spatial division of industries characteristics and trends

Quantitative differentiation of dendritic cells in lung tissues of smokers with and without chronic obstructive pulmonary disease

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Chinese Medical Journal 2010年第12期123卷 1500-1504页

作者： SU Yan-wei xu yong-jian LIU Xian-sheng Department of Respiratory Medicine Tongji Hospital affiliated to Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430030 China

Background Chronic obstructive pulmonary disease （COPD） is thought to be an inflammatory immune response disease. In most cases, the disease is caused by cigarette smoke, but it has been demonstrated that only 10% to 20% of smokers will definitely suffer from COPD. Dendritic cells （DCs） are considered to be the promoter of immune ***, the underlying mechanisms involved are still unrevealed. In this study, we aimed to investigate the quantitative differentiation of pulmonary DC in smokers with or without COPD to explore the possible role of DCs in smokers suffering *** Peripheral lung specimens from non-smokers without airflow obstruction （control group, n=7）, smokers without airflow obstruction （smoker group, n=7） and patients with COPD （COPD group, n=7） were investigated to detect the quantity of S-100 and CD1a positive cells by immunohistochemical or immunofluorescent *** In smokers with COPD, the number of S-100＋ DCs was higher than in the controls and smokers without COPD （P 〈0.01 and P 〈0.05） and there was a higher number of S-100＋ DCs in smokers with COPD than in smokers without COPD, but without a significant difference （P 〉0.05）. An inverse correlation was found between the number of DCs and forced expiratory volume in the first second （FEV1）% pred （r=-0.75, P 〈0.05）, which was also found between the number of DCs and FEV1/forced vital capacity （FVC）（r=-0.72, P 〈0.05）. The mean number of CD1a＋ DCs, increased from non-smokers to non-COPD smokers to COPD patients, with significant differences between each group （P 〈0.01）.Conclusions The quantity of DCs significantly increased in smokers with COPD compared with non-smokers or smokers without COPD. The results suggest that DCs may play an important role in the pathogenesis of smoking-induced COPD, and the upregulation of DCs may be a potential maker to identify the smokers who have more liability to suffer from COPD.

关键词： dendritic cells smoking chronic obstructive pulmonary disease

Effect of cigarette smoke extract on proliferation of rat pulmonary artery smooth muscle cells and the relevant roles of protein kinase C

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Chinese Medical Journal 2007年第17期120卷 1523-1528页

作者： HU Jing xu yong-jian ZHANG Zhen-xiang TIAN Feng Department of Respiratory Medicine Tongji Hospital Affiliated toTongji Medical College Huazhong University of Science andTechnology Wuhan 430030 China

Background Increased proliferation of pulmonary vascular cells and muscularisation of pulmonary vessels are frequently observed in human smokers and in animals exposed to cigarette smoke. To elucidate the molecular mechanisms leading to these changes, we studied the in vitro effect of cigarette smoke extract （CSE） on proliferation of pulmonary artery smooth muscle cells （PASMCs） and activation of protein kinase C （PKC）, an important kinase implicated in cell proliferation. Methods PASMCs cultured from 12 normal Wistar rats were studied in the following conditions：（1） PASMCs were exposed to different concentrations of CSE for 24 hours, then MTT colorimetric assay was used for detection of cell proliferation. Cell viability was assessed by trypan blue exclusion. （2） PASMCs were pre-incubated with phorbol 12-myristate 13-acetate （PMA） for 24 hours or Ro31-8220 for 30 minutes before exposure to 5% CSE for 24 hours. Cell proliferation was examined by MTT colorimetric assay, cell cycle analysis and proliferating cell nuclear antigen （PCNA） immunocytochemical staining. （3） PASMCs were exposed to 5% CSE for 24 hours. Then PKC-a mRNA expression was detected by reverse transcription-polymerase chain reaction （RT- PCR） and protein expression by Western blotting, while PKC-α translocation was observed by immunofluorescence staining and confocal microscopy. （4） PASMCs were transfected with specific antisense oligodeoxynucleotides against PKC-a 6 hours before exposure to 5% CSE for 24 hours. PKC-α protein expression and cell proliferation were detected by methods described previously. Results （1） Low concentration of CSE （5%） increased proliferation of PASMCs, whereas high concentrations （20%, 30%） were inhibitory as a result of cytotoxicity. （2） The value of absorbance （Value A）, proliferation index （PI）, S-phase cell fraction （SPF） and average optical density of PCNA staining in PASMCs from 5% CSE exposure group （0.306 ± 0.033, 0.339 ± 0.033, 0.175 ± 0.021, 0.315 ± 0.038,

关键词： tobacco smoke pollution protein kinase C pulmonary artery muscle, smooth cell proliferation