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Key minerals influencing apple quality in Chinese orchard identified by nutritional diagnosis of leaf and soil analysis

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Journal of Integrative Agriculture 2015年第5期14卷 864-874页

作者： WANG Guo-yi ZHANG Xin-zhong WANG Yi xu xue-feng HAN Zhen-hai Institute for Horticultural Plants China Agricultural University Yantai Research Institute China Agricultural University

We investigated the correlation between leaf/soil minerals and fruit quality in apple trees grown in orchards,with the ultimate goal of improving the *** mineral nutrients;soil nutrients in the 0-20,20-40,and 40-60 cm layers;and fruit quality traits in 32 apple orchards in China were monitored for 2 *** factors associated with fruit quality were identified via correlation *** analysis of leaf data revealed that leaf nitrogen（N） and leaf magnesium（Mg） levels were extremely high in 75 and 89%of the orchards,*** the Bohai Gulf region,94%of the orchards showed significantly higher values than the *** soil pH values of the orchards in eastern China like eastern Shandong or Liaoning were lower than 7.0,while the pH values in the Loess Plateau of northwestern China like Shaanxi were much higher than *** alkali-hydrolyzable N levels in 47%of the orchards were lower than the optimal level of 70 mg kg^-***,the soil alkali-hydrolyzable N levels of orchards in the Bohai Gulf region were significantly higher than those in the Loess Plateau *** available P levels in the orchards of the Bohai Gulf region were up to three times higher than those of the Loess Plateau ***,although the available potassium（K） in most orchards was sufficient（51.39-309.94 mg kg^-1）,leaf K content in 73%of the orchards was low,possibly due to fruit bagging or fruit *** 63%of the orchards in Shandong and 29%of the orchards in Shannxi showed leaf Fe *** the Loess Plateau,most orchards showed high leaf Ca levels,a strong correlation was observed between leaf and soil phosphorus/potassium（P/K）content and fruit organic acid *** amounts of fruit soluble sugar or fructose were positively correlated with soil calcium/potassium（Ca/K） levels and leaf calcium/boron（Ca/B） levels in most *** excessive leaf N levels caused by the extensive application of N fertilizers had a negative effect on

关键词： apple nutrients minerals potassium phosphorus fruit Plateau Bohai monitored fructose

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Effects of NaCl and Iso-Osmotic Polyethylene Glycol Stress on Na^+/H^+Antiport Activity of Three Malus species with Different Salt Tolerance

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Journal of Integrative Agriculture 2014年第6期13卷 1276-1283页

作者： YANG Hong-bing DONG Chun-hai xu xue-feng WANG Yi HAN Zhen-hai Key Lab of Plant Biotechnology in Universities of Shandong/College of Life Sciences Qingdao Agricultural University Stress Physiology and Molecular Biology Laboratory of Fruit Trees China Agricultural University

Salt stress contains osmotic and ionic stress, while iso-osmotic polyethylene glycol （PEG） has only osmotic stress. This study aimed to compare the different effects on the activity of H＋-ATPase, proton pump and Na＋/H＋antiport in Malus seedlings between osmotic and ionic stress. Species of salt tolerant Malus zumi, middle salt tolerant Malus xiaojinensis and salt sensitive Malus baccata were used as experimental materials. Malus seedlings were treated with NaCl and iso-osmotic PEG stress. The activity of H＋-ATPase, proton pump and Na＋/H＋antiport of plasmolemma and tonoplast in Malus seedlings were obviously increased under salt stress, and those in salt-tolerant species increased more. Under the same NaCl concentration, the activity of H＋-ATPase, proton pump and Na＋/H＋antiport of plasmolemma and tonoplast in salt-tolerant species were all obviously higher than those in salt-sensitive one. Higher Na＋/H＋antiport activity of plasmolemma and tonoplast in salt-tolerant species could help to extrude and compartmentalize sodium in roots under salt stress. The ascent rate of activity of H＋-ATPase, proton pump and Na＋/H＋antiport in Malus seedlings under the three salt concentration stress was all obviously higher than that under the iso-osmotic PEG stress. It indicated that the sodium ion effect had more stimulation on the activity of H＋-ATPase, proton pump and Na＋/H＋antiport in salt-tolerant species, and salt-tolerant species has higher capability of sodium extrusion and compartmentalization in roots and is therefore more salt tolerant.

关键词： Malus NaCl polyethylene glycol (PEG) Na+/H+ antiport activity salt tolerance

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Influence of drought hardening on the resistance physiology of potato seedlings under drought stress

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Journal of Integrative Agriculture 2018年第2期17卷 336-347页

作者： ZHANG Shu-han xu xue-feng SUN Ye-min ZHANG Jun-lian LI Chao-zhou Gansu Key Laboratory of Crop Genetics & Germplasm Enhancement College of Life Sciences and Technology Gansu Agricultural University College of Horticulture Gansu Agricultural University

In this paper, the influence of drought hardening on the growth, development, resistance physiology, leaf microstructure and stomatal behavior of potato seedlings under drought stress was studied, and the mechanism of drought hardening improvement of potato seedling drought resistance was elucidated. We found that drought stress had several adverse effects on potato seedlings, yet drought hardening alleviated the decrease in relative water content（RWC）, net photosynthetic rate（Pn） and chlorophyll content and inhibited the increase in relative electric conductivity and malondialdehyde（MDA） content. Compared with contrast seedlings, drought-hardened seedlings also had enhanced root vigor, increased antioxidant enzyme activity and higher levels of abscisic acid（ABA）, proline（Pro）, soluble sugars and polyamines（PAs） under drought stress. In addition, the stomatal density of potato seedling leaves increased significantly, while the leaf area, stomatal size and stomatal aperture decreased with drought hardening treatment. These changes led to reduced leaf transpiration rate（Tr） and improved water utilization efficiency（WUE）. The changes in leaf microstructure also had a positive effect on the drought resistance of the drought-hardened potato seedlings. So it can be concluded that through increasing the content of some endogenous hormones, osmotic regulatory substances and the activities of antioxidant enzymes, the resistance physiology of drought-hardened potato seedlings was enhanced.

关键词： drought drought hardening potato resistance physiology

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Rapamycin in fibrotic diseases： beneficial or detrimental agent？

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Chinese Medical Journal 2013年第7期126卷 1364-1369页

作者： xu xue-feng DAI Hua-ping Department of Respiratory and Critical Care Medicine Beijing Chao-Yang Hospital Capital Medical University Beijing 100020 China Beijing Key Laboratory of Respiratory and Pulmonary Circulation Disorders Beijing Institute of Respiratory Medicine Beijing 100020 China

apamvcin was first isolated from a strain of Sreptomyces hygroscopicus in the early 1970s froma soil sample taken on Easter Island. Because the antiproliferative effects of rapamycin on yeast cell, as well as B and T lymphocytes, it was first identified as an antimicrobial agent with potent immunosuppressive activity and has been used in antirejection therapy.

关键词： fibrotic disease rapamycin mammalian target of rapaymycin negative feedback loop antifibrogenesis

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Epigenetics in neonatal diseases

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Chinese Medical Journal 2010年第20期123卷 2948-2954页

作者： xu xue-feng DU Li-zhong Children's Hospital Zhejiang University School of Medicine Hangzhou Zhejiang 310003 China

Objective To review the role of epigenetic regulation in neonatal diseases and better understand Barker＇s ＂fetal origins of adult disease hypothesis＂.Data sources The data cited in this review were mainly obtained from the articles published in Medline/PubMed between January 1953 and December *** selection Articles associated with epigenetics and neonatal diseases were *** There is a wealth of epidemiological evidence that lower birth weight is strongly correlated with an increased risk of adult diseases, such as type 2 diabetes mellitus, hypertension, and cardiovascular disease. This phenomenon of fetal origins of adult disease is strongly associated with fetal insults to epigenetic modifications of genes. A potential role of epigenetic modifications in congenital disorders, transient neonatal diabetes mellitus （TNDM）, intrauterine growth retardation （IUGR）, and persistent pulmonary hypertension of the newborn （PPHN） have been *** Acknowledgment of the role of these epigenetic modifications in neonatal diseases would be conducive to better understanding the pathogenesis of these diseases, and provide new insight for improved treatment and prevention of later adult diseases.

关键词： epigenetics congenital disorders transient neonatal diabetes mellitus intrauterine growth retardation persistent pulmonary hypertension newborn

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Type 2 epithelial mesenchymal transition in vivo： truth or pitfalls？

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Chinese Medical Journal 2012年第18期125卷 3312-3317页

作者： xu xue-feng DAI Hua-ping Beijing Key Laboratory of Respiratory and Pulmonary Circulation Beijing Institute of Respiratory Medicine Faculty of RespiratoryMedicine Capital Medical University Department of Respiratoryand Critical Care Medicine Beijing Chao-Yang Hospital CapitalMedical University China Beijing 100020

Epithelial-mesenchymal transition （EMT） is a process by which fully differentiated epithelial cells undergo a phenotypic conversion and assume a mesenchymal cell phenotype, including elongated morphology, enhanced migratory and invasiveness capacity, and greatly increased production of extracellular matrix （ECM） components. The EMTs associated with wound healing, tissue regeneration, and organ fibrosis are termed as type 2 EMT. Over the past two decades, emerging evidence suggested that injured epithelial cells, via type 2 EMT, may serve as important sources of fibroblasts and contribute to organ fibrosis, such as kidney, liver, lung and eyes. There is perhaps no doubt that adult epithelial cells can undergo EMT in vitro in response to transforming growth factor （TGF）-131 and other inflammatory or pro-fibrotic stimuli. However, whether type 2 EMT really occurs in vivo, whethers it is actually a source of functional and activated interstitial fibroblasts and whether it contributes to tissue fibrosis have already been the subjects of heated debate. In this review, we will describe the main features of EMT, the major findings of type 2 EMT in vitro, the evidences for and against type 2 EMT in vivo and discuss the heterogeneity and pitfalls of the techniques used to detect EMT during fibrotic diseases. We suggest that in order to ascertain the existence of type 2 EMT in vivo, different proper phenotype markers of epithelial and mesenchymal cells should be jointly used and cell lineage tracking techniques should be standardized and avoid false positives. Finally, we believe that if EMT really occurs and contributes to tissue fibrosis, efforts should be made to block or reverse EMT to attenuate fibrotic process.

关键词： epithelial mesenchymal transition tissue fibrosis fibroblast

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Object-oriented GIS technology and mine geo-dynamic division information management

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中国有色金属学会会刊：英文版 2005年第S1期15卷 42-44页

作者： LI Sheng, feng Fu, xu xue-feng (Liaoning Technical University, Fuxin 123000, China)

Object-oriented methodology was expected to revolutionize GIS design and development, making it easier and faster to design, through a common approach to conceptual modelling, database design, programming languages and user interface tools. In addition, an object-oriented model provides some useful tools for data abstraction and data structuring, which augment the conventional tools and overcome some deficiencies inherent to the traditional relational model. The principles of object-oriented modelling applied to geographic data were put forward, and the concepts of object-oriented paradigm and characteristics of spatial data management were proposed in order to argument their compatibility.

关键词： object-oriented GIS geo-dynamic division

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Wound healing mechanisms and regulation of pulmonary fibrosis： how far are we from the hilltop？

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Chinese Medical Journal 2011年第24期124卷 4125-4126页

作者： xu xue-feng DAI Hua-ping WANG Chen Beijing Key Laboratory of Respiratory and Pulmonary Circulation Beijing Institute of Respiratory Medicine Department of Respiratory and Critical Care Medicine Beijing Chao-YangHospital Capital Medical University Beijing 100020 China （XuXF Dai HP and Wang C） Beijing Hospital Ministry of Health of China Beijing 100730China （Wang C）

L ungs are located in the chest and lie on both sides of mediastinum. The major balance the external and internal function of lung is to ‘gas＇ environment and to maintain homeostatic equilibrium. Because lung is closely interacted with outside surroundings as well as general circulation, it is fair to say that lung is a delicate organ which may be attacked by so many noxious stimuli,

关键词： pulmonary fibrosis IPF Organ fibrosis mechanisms

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Polymorphism analysis of the ABCA3 gene： association with neonatal respiratory distress syndrome in preterm infants

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Chinese Medical Journal 2012年第9期125卷 1594-1598页

作者： JIANG Lin WU Yi-dong xu xue-feng DU Li-zhong Department of Neonatology Children's HospitalZhejiang University School of MedicineHangzhouZhejiang 310003China

Background Previous reports indicated that mutations in the adenosine triphosphate （ATP）-binding cassette transporter A3 （ABCA3） cause fatal respiratory failure in term infants, and common ABCA3 gene polymorphisms have been characterized at the population level in Caucasians. But the role of ABCA3 in relation to respiratory distress syndrome （RDS） in newborns has not been evaluated within a Chinese population. The aim of this study was to analyze eight single-nucleotide polymorphisms （SNPs） of the ABCA3 gene, and to assess the ABCA3 gene as a candidate gene for susceptibility to RDS in newborns.Methods Eight SNPs were selected and genotyped in 203 newborns. The data analysis and statistical tests were used for allele frequencies, haplotype and Hardy-Weinberg equilibrium pairwise linkage disequilibrium measures. Results There was a haplotype association with SNP rs313909 and SNP rs170447, but no haplotype association was observed among the newborns with and without RDS （P 〉0.05）. The minor allele frequency （G） of the coding SNP （cSNP） rs323043 （P585P） was significantly increased in preterm infants with RDS.Conclusion There is an association between a synonymous cSNP rs323043 and the development of RDS.

关键词： adenosine triphosphate-binding cassette transporter A3 respiratory distress syndrome single-nucleotide polymorphisms minor allele frequency

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钢筋混凝土L形截面柱的曲率延性

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华南理工大学学报（自然科学版） 2009年第6期37卷 136-141,146页

作者：蔡健许雪峰华南理工大学土木工程系广东广州510640

在充分考虑荷载角对L形截面柱曲率延性影响的基础上,提出两种曲率延性的概念.通过对10800根L形截面柱的曲率延性进行模拟计算,得出了各因素对曲率延性的影响规律:中和轴角及轴压比对曲率延性的影响最大;体积配箍率的增加从整体上提高了... 详细信息

在充分考虑荷载角对L形截面柱曲率延性影响的基础上,提出两种曲率延性的概念.通过对10800根L形截面柱的曲率延性进行模拟计算,得出了各因素对曲率延性的影响规律:中和轴角及轴压比对曲率延性的影响最大;体积配箍率的增加从整体上提高了L形截面柱的曲率延性;在轴压比不变的情况下,混凝土强度和纵筋强度对曲率延性的影响不大;随着L形率的减小,曲率延性曲线趋于平滑.文中还建立了中和轴角曲率延性及荷载轴角曲率延性的计算公式.

关键词： L形截面柱钢筋混凝土柱中和轴角荷载角曲率延性

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