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Photoperiod Mediates the Effects of Temperature and Light Intensity on the Proliferation of Ulva prolifera

Journal of Ocean University of China 2024年第1期23卷 255-263页

作者： JIANG Jianan YU Yanyan CHEN Yili LI Yahe xu nianjun Key Laboratory of Marine Biotechnology of Zhejiang Province School of Marine SciencesNingbo UniversityNingbo 315211China

In order to study the complex effects of photoperiod,temperature,and light intensity on the spore maturation and release number of Ulva prolifera,we cultured thalli segment(2–3 mm)under three different photoperiods(L:D=12:12,14:10 and 10:14),temperature(15℃(LT),25℃(MT)and 30℃(HT))and light intensity(100,200 and 400μmol m^(−2)s^(−1),noted as LL,ML and HL,respectively)*** the maturation time,spore release number and chlorophyll fluorescence were *** results suggested that:1)The spore maturation time was accelerated by higher temperature or higher light intensity from 62 h to 36 h,and changes in day length accelerated the spore maturation to a certain extent as compared with 12:12 light/dark cycle;2)Higher light intensity significantly decreased the chlorophyll fluorescence(Fv′/Fm′,NPQ,rETRmax andα)of the mature reproductive segment under 30℃with 12:12 light/dark *** when in the other photoperiods(10:14 and 14:10 conditions),the inhibitory effects of high light intensity were alleviated significantly;3)The optimum condition for the spore maturation and release was 12:12 light/dark cycle,25℃,400μmol m^(−2)s^(−1),with both shorter and longer photoperiod reducing the spore release number;4)Higher light intensity significantly increased the spore release number under 25℃,but these effects were alleviated by 30℃*** study is the first attempt to elucidate the coincidence effects of photoperiod,temperature and light intensity on the reproduction of Ulva,which would help to reveal the mechanism of the rapid proliferation of green tide.

关键词： light intensity maturation photoperiod reproduction temperature Ulva prolifera

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海藻糖对高温胁迫龙须菜的生理及基因表达的影响

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水产学报 2024年第6期48卷 157-168页

作者：陈文康张莹莹卢俊徐年军孙雪宁波大学海洋学院浙江省海洋生物工程重点实验室

为了探讨海藻糖在大型藻类抗逆中的作用及其机制，实验利用生理生化分析、高效液相色谱-串联质谱(LC-MS/MS)、荧光定量PCR (qRT-PCR)方法分析了外源海藻糖对高温胁迫下大型海藻龙须菜生长、抗逆相关物质和酶活性、植物激素及相关基因表... 详细信息

为了探讨海藻糖在大型藻类抗逆中的作用及其机制，实验利用生理生化分析、高效液相色谱-串联质谱(LC-MS/MS)、荧光定量PCR (qRT-PCR)方法分析了外源海藻糖对高温胁迫下大型海藻龙须菜生长、抗逆相关物质和酶活性、植物激素及相关基因表达的影响。结果发现，外源添加10 mmol/L海藻糖使龙须菜的相对生长速率和脯氨酸含量分别升高0.34倍(3 d)和0.30倍(24 h)，叶绿素荧光参数、超氧化物歧化酶和海藻糖-6-磷酸合成酶活性升高，但丙二醛含量和脂氧合酶(LOX)活性降低。同时，海藻糖激活了龙须菜中水杨酸代谢关键酶——异分支酸合成酶的活性及其基因表达，促进了水杨酸的积累(2.05倍)。此外，海藻糖可以使热激蛋白70基因表达稳定升高、lox2基因表达下调(12 h)。可见，外源海藻糖可以通过清除活性氧自由基，降低膜脂过氧化，促进渗透保护物质和抗逆植物激素水杨酸的积累等来促进高温下龙须菜的生长。本研究证明了海藻糖在龙须菜抗高温胁迫中具有重要作用，为龙须菜的抗逆育种工作提供了参考。

关键词：龙须菜海藻糖高温生理基因表达

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Effects of polyamines on the early development of cystocarps,spore release,survival,and germination rate of Gracilariopsis lemaneiformis

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Journal of Oceanology and Limnology 2023年第3期41卷 1133-1144页

作者： Deguang SUN Emon RAHMAN Siqi SUN xue SUN nianjun xu Key Laboratory of Marine Biotechnology of Zhejiang Province School of Marine SciencesNingbo UniversityNingbo 315211China

Three polyamines(PAs)(spermidine(Spd),spermine(Spm),and putrescine(Put))were used as growth regulators to the marine macroalgae Gracilariopsis lemaneiformis and to female gametophyte and reproductive tissues(cystocarps were constructed),which led to early maturation of cystocarps and spore *** cultivation revealed that exogenous PA application accelerated the development of cystocarps,and the mean sporeling growth rate was 20%–50%/d during the initial week of *** spore count(SC),survival rate(SR),and germination rate(GR)of *** showed different responses to PA(10-6 mol/L and 10-3 mol/L)treatments under different temperatures(18,26,and 34℃),light intensities(30,60,and 90μmol photons/(m^(2)·s)),salinities(25,30,and 35),and nutrient levels(25꞉2.5,50꞉5.0,and 75꞉7.5μmol/L NH4Cl꞉K2HPO4),and the optimal conditions were 26℃,light intensity of 60μmol/(m^(2)·s),salinity of 35,and nutrient level of 50꞉5.0μmol/L NH4Cl꞉*** the optimum conditions,the SC per cystocarp increased by 82.38%,and the spore SR and GR increased by 33.07%and 46.44%,*** experiment lasted for 7 days,with the highest SC on Day 4 and plateauing on Day *** results indicate that PAs could promote the early maturation,spore release,survival,and germination of *** under laboratory conditions.

关键词： Gracilariopsis lemaneiformis polyamines carpospores spore release survival rate germination rate

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Sodium acetate can promote the growth and astaxanthin accumulation in the unicellular green alga Haematococcus pluvialis as revealed by a proteomics approach

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Journal of Oceanology and Limnology 2022年第5期40卷 2052-2067页

作者： Xinlei YU Xin YE Chaoyang HU nianjun xu xue SUN Key Laboratory of Marine Biotechnology of Zhejiang Province School of Marine SciencesNingbo UniversityNingbo 315211China

Haematococcus pluvialis is an ideal natural source of strong antioxidant *** acetate(NaAc)was proven an effective organic carbon source for improving algal growth and astaxanthin production;however,the underlying mechanism remains *** reveal the mechanism of NaAc at the green vegetative stage of ***,the physiochemical characteristics and the global protein expression profiles obtained using a tandem mass tag labeling approach were compared between the control(CK)and two NaAc-addition *** show that after NaAc addition,the biomass,nitrate consumption rate,and activities of three carbohydrate metabolism enzymes of *** were significantly increased,and the net photosynthetic rate and chlorophyll content *** addition,astaxanthin,total carbohydrates,and total lipids were accumulated,and some red cells appeared in the NaAc5 ***,317 differentially expressed proteins(DEPs)with the most altered expression patterns were screened out in the CK ***5 comparison in our proteomics *** the DEPs involved in carbohydrate metabolism and lipid metabolism were significantly increased,while most of the photosynthesis-related proteins were depressed in the two NaAc-treated *** proteomics results were verified and supported by parallel reaction monitoring approach and physiochemical *** findings demonstrate that NaAc promoted the tricarboxylic acid cycle,glyoxylate cycle,and amino acid and lipid synthesis,and inhibited the photo synthe sis-related activities,which consequently speeded up the growth and astaxanthin accumulation in this alga.

关键词： Haematococcus pluvialis sodium acetate(NaAc) growth differentially expressed protein proteomics approach

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Changes in Growth, Carbon and Nitrogen Enzyme Activity and mRNA Accumulation in the Halophilic Microalga Dunaliella viridis in Response to NaCl Stress

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Journal of Ocean University of China 2016年第6期15卷 1094-1100页

作者： WANG Dongmei WANG Weiwei xu nianjun SUN xue Key Laboratory of Applied Marine Biotechnology of Ministry of Education School of Marine SciencesNingbo University Ningbo 315211 P. R. China

Many species of microalga Dunaliella exhibit a remarkable tolerance to salinity and are therefore ideal for probing the effects of salinity. In this work, we assessed the effects of NaCl stress on the growth, activity and m RNA level of carbon and nitrogen metabolism enzymes of D. viridis. The alga could grow over a salinity range of 0.44 mol L^(-1) to 3.00 mol L^(-1) NaCl, but the most rapid growth was observed at 1.00 mol L^(-1) NaCl, followed by 2.00 mol L^(-1) NaCl. Paralleling these growth patterns, the highest initial and total Rubisco activities were detected in the presence of 1.00 mol L^(-1) NaCl, decreasing to 37.33% and 26.39% of those values, respectively, in the presence of 3.00 mol L^(-1) Na Cl, respectively. However, the highest extracellular carbonic anhydrase(CA) activity was measured in the presence of 2.00 mol L^(-1) NaCl, followed by 1.00 mol L^(-1) NaCl. Different from the two carbon enzymes, nitrate reductase(NR) activity showed a slight change under different NaCl concentrations. At the transcriptional level, the m RNAs of Rubisco large subunit(rbcL), and small subunit(rbc S), attained their highest abundances in the presence of 1.00 and 2.00 mol L^(-1)NaCl, respectively. The CA mRNA accumulation was induced from 0.44 mol L^(-1) to 3.00 mol L^(-1) NaCl, but the NR m RNA showed the decreasing tendency with the increasing salinity. In conclusion, the growth and carbon fixation enzyme of Rubisco displayed similar tendency in response to NaCl stress, CA was proved be salt-inducible within a certain salinity range and NR showed the least effect by NaCl in D. viridis.

关键词： NaCl胁迫 mRNA水平杜氏盐藻酶活性活性碳 Rubisco活性经济增长积累

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A new approach to promote astaxanthin accumulation via Na_2WO_4 in Haematococcus pluvialis

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Journal of Oceanology and Limnology 2019年第1期37卷 176-185页

作者： LI Yanguo CUI DANDan ZHUO Pinli ZHANG Lin SUN xue xu nianjun Key Laboratory of Marine Biotechnology of Zhejiang Province School of Marine Sciences Ningbo University

The optimum concentration of Na_2 WO_4 was explored in relation to the cell density and astaxanthin content in Haematococcus pluvialis. Then, the cellular morphology, nitrate reductase(NR) activity, soluble sugar and protein contents, and chlorophyll ?uorescence were measured, and the transcriptional expression of carotenogenic genes was determined by quantitative real-time PCR. The results showed that 3.0 mmol/L of Na_2 WO_4 was the optimum concentration to induce astaxanthin accumulation, with a maximum content of 49.41±0.13 pg/cell reached on the tenth day. The NR activity decreased signi?cantly and continually after Na_2 WO_4 treatment. The soluble sugar content increased gradually during the experimental period and was eventually signi?cantly higher than that in the control. The soluble protein content increased rapidly,reached a maximum in day 0.5 and day 1 and then decreased. The ef fective photochemical effciency of PSII( F v'/F m') and light saturation( E k) ?rst decreased and then tended to stabilize, and NADP +-glyceraldehyde-3-phosphate dehydrogenase(GAPDH) gene expression was correlated with photosynthesis. The transcriptional expression of ipi, psy and bkt was signi?cantly increased compared with that in the control after application of Na_2 WO_4, and the relative expression of ipi reached the highest level on the ?fth day, with a 98.03±1.92-fold increase. Our results describe a new approach to promote the ef fective accumulation of astaxanthin in H. pluvialis by NR inhibitor Na_2 WO_4.

关键词： Haematococcus pluvialis astaxanthin Na2WO4 nitrate reductase quantitative real-time PCR

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Species Composition and Diversity of Macrobenthos in the Intertidal Zone of Xiangshan Bay,China

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Journal of Ocean University of China 2015年第2期14卷 375-384页

作者： JIAO Haifeng ZHENG Dan YOU Zhongjie xu nianjun LOU Dan HUANG Chengwei School of Marine Science Ningbo University Ningbo Academy of Oceanology and Fishery

Xiangshan bay is a narrow semi-closed bay and situated on the northwestern coast of the East China Sea. Over past decades, it has become to a major bay with intensive human activities, dense urbanized area, and poor water quality. The aim of this paper was to reveal the ecological status through the elucidation of the species composition, abundance, biomass and diversity of macrobenthos in this bay. Six intertidal sections were surveyed from January 2007 to November 2008 quarterly. Sections TG, HD and XH are located in the three inner bays, sections QJ and WS are located near the thermal power plants, and section XX is located at the outer part of Xiangshan Bay. Great variations in macrobenthos community were indentified, and the species composition of the community in the present study showed the dominance in the order of molluscs(bivalves and gastropods), crustaceans and others, and only few Polychaeta were recorded. Only three dominant species, Littorina brevicula, Ilyplax tansuiensis, and Cerithidea cingulata were collected in all the sections, and a total of 19 dominant species were recorded only in one section. Two-way ANOVA analyses of abundance indicated that there were significant differences among sections or seasons. Shannon-Wiener diversity index(H') had its maximum(2.45) in section QJ, and minimum(1.76) in section TG. Multiple irregular k-dominance plots clearly showed that the study area was polluted and the macrobenthos community was under stress. We conclude that the macrobenthos of Xiangshan Bay have been disturbed by human activities, especially at the interior bay.

关键词：动物多样性物种组成中国东海大型底栖动物群落潮间带人类活动城市化地区甲壳类动物

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海洋微生物的化学生态学研究进展

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应用生态学报 2006年第12期17卷 2436-2440页

作者：徐年军严小军宁波大学生命科学与生物工程学院海洋生物工程重点实验室宁波315211

近年来,海洋生物的化学生态学研究已成为国际化学生态学研究的亮点之一.该领域的研究不仅为生物进化研究提供了理论依据,也对海洋生态养殖、海洋生态环境保护以及海洋资源的可持续发展具有重要意义.本文从海洋动物、植物、微生物三方面... 详细信息

近年来,海洋生物的化学生态学研究已成为国际化学生态学研究的亮点之一.该领域的研究不仅为生物进化研究提供了理论依据,也对海洋生态养殖、海洋生态环境保护以及海洋资源的可持续发展具有重要意义.本文从海洋动物、植物、微生物三方面综述了它们与海洋微生物之间的化学生态学关系.海洋动物与微生物的化学生态学作用主要包括抗菌、抗附着、共生3种关系.以发现具有生态学效应的化学信号物质的分子结构为主线,介绍了海洋植物和微生物方面的研究进展,并对该领域的关键性问题和发展方向进行了展望.

关键词：化学生态学海洋化学生态学海洋次生物质

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低盐胁迫下模拟酸雨对裂片石莼光合作用生理特性的影响

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水产学报 2016年第5期40卷 731-739页

作者：徐晓婷李亚鹤王东徐年军宁波大学教育部应用海洋生物技术重点实验室浙江省海洋生物工程重点实验室

为探讨低盐胁迫下模拟酸雨对裂片石莼光合作用、抗氧化活性等生理特性的影响,实验设置3个盐度梯度、2个p H水平,运用叶绿素荧光仪、液相氧电极等仪器测定叶绿素荧光参数和光合放氧速率,采用氮蓝四唑法、考马斯亮蓝G250法及蒽酮硫酸比色... 详细信息

为探讨低盐胁迫下模拟酸雨对裂片石莼光合作用、抗氧化活性等生理特性的影响,实验设置3个盐度梯度、2个p H水平,运用叶绿素荧光仪、液相氧电极等仪器测定叶绿素荧光参数和光合放氧速率,采用氮蓝四唑法、考马斯亮蓝G250法及蒽酮硫酸比色法测定超氧化物歧化酶(SOD)活性、可溶性蛋白和糖含量。结果发现:(1)模拟酸雨(pH 4.4)处理显著抑制了裂片石莼的生长,且受盐度的影响。p H 8.1、盐度10处理的裂片石莼具有较高的生长速率;盐度越高,模拟酸雨的影响越大。(2)模拟酸雨(pH 4.4)处理显著抑制了裂片石莼的最大光合效率(F/F)和有效光化学效率(F′/F′),但受盐度的影响不显著;模拟酸雨和低盐度协同降低了裂片石莼的最大相对电子传递速率(rETRmax)。(3)模拟酸雨(pH 4.4)降低了裂片石莼的暗呼吸和光合放氧速率,但受盐度的影响不显著。(4)正常盐度及正常p H处理的裂片石莼SOD活性最高;在盐度(10、25)处理下,模拟酸雨抑制了裂片石莼的SOD活性;而在盐度5处理时,模拟酸雨提高了SOD活性。(5)相对于海水正常p H处理,模拟酸雨显著降低了裂片石莼可溶性蛋白含量,但其可溶性糖含量显著提高,尤其是在低盐条件下。研究表明,模拟酸雨对裂片石莼生长的影响受海水盐度的调控,这在一定程度上也将影响近岸海域大型海藻的生态群落组成。

关键词：裂片石莼低盐模拟酸雨光合作用抗氧化活性

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壳聚糖酶法降解工艺优化及其产物的抗氧化活性

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中国食品学报 2017年第11期17卷 121-130页

作者：李汶潓李亚鹤徐年军沈燕萍宁波大学海洋学院浙江省海洋生物工程重点实验室浙江宁波315211 教育部应用海洋生物技术重点实验室浙江宁波315211

在单因素试验的基础上,运用响应面分析法优化壳聚糖酶法催化成壳寡糖的条件及其酶解产物DPPH自由基清除活性。根据单因素试验结果固定壳聚糖底物质量浓度为2 g/100 m L,选取不同酶底比、酶解时间、壳聚糖溶液p H值和酶解温度作自变量,... 详细信息

在单因素试验的基础上,运用响应面分析法优化壳聚糖酶法催化成壳寡糖的条件及其酶解产物DPPH自由基清除活性。根据单因素试验结果固定壳聚糖底物质量浓度为2 g/100 m L,选取不同酶底比、酶解时间、壳聚糖溶液p H值和酶解温度作自变量,以酶解产物清除DPPH自由基能力为响应值,应用Box-Behnken中心组合法进行四因素三水平试验设计。研究结果表明,4个因素对酶解产物DPPH自由基清除能力的影响为:壳聚糖溶液p H值﹥酶解反应温度﹥酶底比﹥酶解反应时间。通过二次回归模型响应面分析酶解产物DPPH自由基清除活性最佳优化条件为酶解反应时间64 min,E/S 36%,壳聚糖溶液p H 4.6,反应温度54℃。壳聚糖酶解产物实际DPPH自由基清除能力达92.60%,与模型理论值92.36%相比,误差为0.26%。由响应面法优化得到的壳聚糖酶解产物具有较好的DPPH自由基清除能力,值得进一步研究与应用。

关键词：壳聚糖酶解产物 DPPH自由基清除活性抗氧化

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