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Chinese Science Bulletin 2006年第13期51卷 1652-1654页

作者： DUN Baoqing LU Wei ZHANG Wei PING Shuzhen WANG xujing CHEN ming xu Yuquan JIN Dan WANG Jin ZHAO Zhonglin LIANG Aimin HOU Songna xu ming-qun LIN Min Biotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing 100081 China New England Biolabs Inc. Beverly Massachusetts 01915 USA

A mutagenesis library was constructed using GPS-LS system to insert a random 5 aa into the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) encoded by aroA gene. Active EPSPS pro- teins were identified by the ability to rescue growth of aroA-deleted mutant ER2799 on M9 minimal media. 12 unique sites, which can tolerate a 5-aa insertion, were identified. In all of the 12 sites, only F295/T296 site was found to split the G2-EPSPS properly by co-transformation of plasmids into E. coli ER2799. The G2-EPSPS gene was then divided into N-termi- nal and C-terminal from F295/T296 site which were fused to the N-terminal and C-terminal of *** intein, respectively, creating two plasmids pMEPS- N295IN and pKEPSc296Ic. Co-transformation of plasmids, pMEPSN295IN and pKEPSc296Ic, rescu- ed growth of ER2799 in M9 minimal media, indicating that the intein splicing domains were bringing the EPSPS fragments together to generate activity. Re- consituted activity of splitted G2-EPSPS enzyme was 4.48 U/mg.

关键词：断裂基因蛋白质再生转基因 EPSPS

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断裂型内含肽在蛋白生产和生物工程中的应用

生物技术通讯 2008年第5期19卷 724-727页

作者：杨帆 xu ming-qun 王永程奕天津市农业科学院中心实验室天津300192 New England Biolab Inc. Ipswich Massachusetts 01938 USA

蛋白反式剪接是蛋白翻译后修饰的一种特殊机制,这一反应由断裂型内含肽自我催化完成,不需要酶和其他因子参与。与常规顺式的蛋白剪接不同的是,反式剪接是基于断裂型内含肽由N端和C端这两段多肽的高亲和力,精准地构建成一个具有剪接活性... 详细信息

蛋白反式剪接是蛋白翻译后修饰的一种特殊机制,这一反应由断裂型内含肽自我催化完成,不需要酶和其他因子参与。与常规顺式的蛋白剪接不同的是,反式剪接是基于断裂型内含肽由N端和C端这两段多肽的高亲和力,精准地构建成一个具有剪接活性的内含肽蛋白质。反式剪接已被发展成新的生物技术应用于生产环化蛋白、构建蛋白定点与定时表达的载体和转基因植物,以及改造cDNA文库技术等。

关键词：断裂型蛋白内含肽反式剪接环化蛋白质 cDNA文库

热毒宁注射液结合阿奇霉素治疗小儿支原体肺炎20例

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中国民族民间医药 2011年第24期20卷 114-115页

作者：徐群明江苏省宜兴市中医医院江苏宜兴214200

目的:观察热毒宁注射液结合阿奇霉素序贯治疗小儿支原体肺炎(MP)的临床疗效。方法:将40例小儿支原体肺炎随机分为治疗组和对照组各20例。两组均予阿奇霉素序贯治疗,治疗组加用中药热毒宁注射液,疗程5～7d。结果:热毒宁注射液结合阿奇霉... 详细信息

目的:观察热毒宁注射液结合阿奇霉素序贯治疗小儿支原体肺炎(MP)的临床疗效。方法:将40例小儿支原体肺炎随机分为治疗组和对照组各20例。两组均予阿奇霉素序贯治疗,治疗组加用中药热毒宁注射液,疗程5～7d。结果:热毒宁注射液结合阿奇霉素组疗效明显优于对照组,差异有显著性(P<0.05)。结论:热毒宁注射液结合阿奇霉素序贯治疗小儿支原体肺炎疗效显著,值得临床推广。

关键词：热毒宁注射液儿科支原体肺炎中西医结合疗法

Deciphering fungal metabolon coupling tandem inverse-electrondemand Diels-Alder reaction and semipinacol rearrangement for the biosynthesis of spiro polycyclic alkaloids

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Science China Chemistry 2025年第1期68卷 288-296页

作者： Shuai Liu Wen-Qiang xu Ying-Tong Di Man-Cheng Tang Ding-Kang Chen ming-ming Cao Yao-Wen Chang Hong-Yu Tang Chun-Mao Yuan Jun-Bo Yang Zhi-Li Zuo Han Guo Zi-Fei xu Ying Zeng Yun-Dong Wu Xiao-Jiang Hao Key Laboratory of Phytochemistry and Natural Medicines (Chinese Academy of Sciences) Kunming Institute of Botany Chinese Academy of Sciences University of Chinese Academy of Sciences Lab of Computational Chemistry and Drug Design State Key Laboratory of Chemical Oncogenomics Peking University Shenzhen Graduate School Shenzhen Bay Laboratory State Key Laboratory of Microbial Metabolism School of Life Sciences & Biotechnology Shanghai Jiao Tong University State Key Laboratory of Functions and Applications of Medicinal Plants Guizhou Medical University Molecular Biology Experimental Center Germplasm Bank of Wild Species in Southwest China Kunming Institute of Botany Chinese Academy of Sciences Yunnan Key Laboratory for Wild Plant Resources Kunming Institute of Botany Chinese Academy of Sciences Research Unit of Chemical Biology of Natural Anti-Virus Products Chinese Academy of Medical Sciences

In the intricate process of natural product biosynthesis, a metabolon can enhance metabolic flux by associating sequential enzymes. A fungal metabolon, comprising of flavin-dependent monooxygenase Spe F and P450 monooxygenase Spe G, is identified in the biosynthesis of spiro polycyclic alkaloids(+)-notoamide B and its diastereomer(+)-versicolamide B. Using notoamide E as a substance, Spe F/Spe G metabolon can control the stereoselectivity of its 2,3-epoxidation, followed by hydrogen atom ion at C-17 to generate reactive epoxide tau-m A with dienyl iminium unit. Subsequently,(+)-notoamide B and(+)-versicolamide B are produced via tandem nonenzymatic inverse-electron-demand Diels-Alder reaction and semipinacol rearrangement. This provides the first example of metabolon in the biosynthesis of spiro-prenylated indole alkaloids.

关键词： metabolon Diels-Alder reaction semipinacol rearrangement biosynthesis notoamides spiro polycyclic alkaloids

Enhancement of motor functional recovery in thoracic spinal cord injury: voluntary wheel running versus forced treadmill exercise

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Neural Regeneration Research 2025年第3期20卷 836-844页

作者： Do-Hun Lee Dan Cao Younghye Moon Chen Chen Nai-Kui Liu Xiao-ming xu Wei Wu Spinal Cord and Brain Injury Research Group Stark Neurosciences Research InstituteIndiana University School of MedicineIndianapolisINUSA Department of Neurological Surgery Indiana University School of MedicineIndianapolisINUSA Department of Neuroscience Baylor College of MedicineHoustonTXUSA

Spinal cord injury necessitates effective rehabilitation strategies, with exercise therapies showing promise in promoting recovery. This study investigated the impact of rehabilitation exercise on functional recovery and morphological changes following thoracic contusive spinal cord injury. After a 7-day recovery period after spinal cord injury, mice were assigned to either a trained group(10 weeks of voluntary running wheel or forced treadmill exercise) or an untrained group. Bi-weekly assessments revealed that the exercise-trained group, particularly the voluntary wheel exercise subgroup, displayed significantly improved locomotor recovery, more plasticity of dopaminergic and serotonin modulation compared with the untrained group. Additionally, exercise interventions led to gait pattern restoration and enhanced transcranial magnetic motor-evoked potentials. Despite consistent injury areas across groups, exercise training promoted terminal innervation of descending axons. In summary, voluntary wheel exercise shows promise for enhancing outcomes after thoracic contusive spinal cord injury, emphasizing the role of exercise modality in promoting recovery and morphological changes in spinal cord injuries. Our findings will influence future strategies for rehabilitation exercises, restoring functional movement after spinal cord injury.

关键词： behavioral assessment motor function neural plasticity running wheel exercise spinal cord injury treadmill exercise voluntary exercise

Regulation and function of endoplasmic reticulum autophagy in neurodegenerative diseases

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Neural Regeneration Research 2025年第1期20卷 6-20页

作者： Xiu-Yun Zhao De-En xu ming-Lei Wu Ji-Chuan Liu Zi-Ling Shi Quan-Hong Ma Department of Neurology and Clinical Research Center of Neurological Disease the Second Affiliated Hospital of Soochow UniversitySuzhouJiangsu ProvinceChina Institute of Neuroscience&Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases Soochow UniversitySuzhouJiangsu ProvinceChina Department of Neurology Jiangnan University Medical CenterWuxiJiangsu ProvinceChina

The endoplasmic reticulum,a key cellular organelle,regulates a wide variety of cellular *** reticulum autophagy,one of the quality control systems of the endoplasmic reticulum,plays a pivotal role in maintaining endoplasmic reticulum homeostasis by controlling endoplasmic reticulum turnover,remodeling,and *** this review,we briefly describe the endoplasmic reticulum quality control system,and subsequently focus on the role of endoplasmic reticulum autophagy,emphasizing the spatial and temporal mechanisms underlying the regulation of endoplasmic reticulum autophagy according to cellular *** also summarize the evidence relating to how defective or abnormal endoplasmic reticulum autophagy contributes to the pathogenesis of neurodegenerative *** summary,this review highlights the mechanisms associated with the regulation of endoplasmic reticulum autophagy and how they influence the pathophysiology of degenerative nerve *** review would help researchers to understand the roles and regulatory mechanisms of endoplasmic reticulum-phagy in neurodegenerative disorders.

关键词： autophagy endoplasmic reticulum endoplasmic reticulum autophagy endoplasmic reticulum quality control system endoplasmic reticulum receptors endoplasmic reticulum-associated degradation neurodegeneration neurodegenerative disease selective autophagy unfolded protein response

Chondroitinase ABC combined with Schwann cell transplantation enhances restoration of neural connection and functional recovery following acute and chronic spinal cord injury

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Neural Regeneration Research 2025年第5期20卷 1467-1482页

作者： Wenrui Qu Xiangbing Wu Wei Wu Ying Wang Yan Sun Lingxiao Deng Melissa Walker Chen Chen Heqiao Dai Qi Han Ying Ding Yongzhi Xia George Smith Rui Li Nai-Kui Liu Xiao-ming xu Spinal Cord and Brain Injury Research Group Stark Neurosciences Research InstituteDepartment of Neurological SurgeryIndiana University School of MedicineIndianapolisINUSA Department of Hand Surgery the Second Hospital of Jilin UniversityChangchunJilin ProvinceChina Shriners Hospitals Pediatric Research Center Temple University School of MedicinePhiladelphiaPAUSA Department of Anatomy&Cell Biology Indiana University School of MedicineIndianapolisINUSA

Schwann cell transplantation is considered one of the most promising cell-based therapy to repair injured spinal cord due to its unique growth-promoting and myelin-forming properties.A the Food and Drug Administration-approved Phase I clinical trial has been conducted to evaluate the safety of transplanted human autologous Schwann cells to treat patients with spinal cord injury.A major challenge for Schwann cell transplantation is that grafted Schwann cells are confined within the lesion cavity,and they do not migrate into the host environment due to the inhibitory barrier formed by injury-induced glial scar,thus limiting axonal reentry into the host spinal *** we introduce a combinatorial strategy by suppressing the inhibitory extracellular environment with injection of lentivirus-mediated transfection of chondroitinase ABC gene at the rostral and caudal borders of the lesion site and simultaneously leveraging the repair capacity of transplanted Schwann cells in adult rats following a mid-thoracic contusive spinal cord *** report that when the glial scar was degraded by chondroitinase ABC at the rostral and caudal lesion borders,Schwann cells migrated for considerable distances in both rostral and caudal *** Schwann cell migration led to enhanced axonal regrowth,including the serotonergic and dopaminergic axons originating from supraspinal regions,and promoted recovery of locomotor and urinary bladder ***,the Schwann cell survival and axonal regrowth persisted up to 6 months after the injury,even when treatment was delayed for 3 months to mimic chronic spinal cord *** findings collectively show promising evidence for a combinatorial strategy with chondroitinase ABC and Schwann cells in promoting remodeling and recovery of function following spinal cord injury.

关键词： axonal regrowth bladder function chondroitinase ABC functional recovery glial scar lentivirus migration Schwann cell spinal cord injury transplantation

A versatile synthetic strategy towards rare earth based metal-organic frameworks

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Science China Chemistry 2025年

作者： Quan Shi Yun-Hao xu ming-Hui Chen Peng Zhao Wen-Bo Deng Ji Xiong Kai Guo Ya-Qing Feng Bao Zhang School of Chemical Engineering and Technology Tianjin University Tianjin Collaborative Innovation Center of Chemical Science and Engineering Guangdong Laboratory Chemistry & Fine Chemical Industry Jieyang Center

Rare earth metal-organic frameworks (RE-MOFs) have drawn wide attention owing to diverse network structures and various application ***,due to the unpredictable formation of the nodes,it is still challenging to design novel RE-MOFs.A novel versatile strategy was developed employing a RE-acetate complex (RE2(CH3COO)6·4H2O) as a precursor to construct RE-MOFs.2D RE-MOFs (RE-DBP) and 3D RE-MOFs (RE-TCPP) could be obtained by using dicarboxylic porphyrin ligands H2DBP and tetracarboxylic porphyrin ligands H2TCPP,*** RE-MOFs were further synthesized to verify the universality of the *** crystalline RE-MOFs could also be prepared at room temperature via the developed strategy,which could significantly promote large-scale preparation and practical application of *** proof of the concept,the performance of porphyrin-based RE-MOFs was investigated in the photocatalytic cycloaddition of CO2with *** the RE-DBP(Co) MOFs,Tb-DBP(Co) exhibited the best photocatalytic performance,which was also greater than that of the corresponding 3D MOF Tb-TCPP(Co) due to the optimal 2D structure and thus the accelerated photogenerated charge separation and transfer *** work not only provides a novel versatile strategy to design and develop RE-MOFs,but also proposes a promising approach for the potential bulk production of RE-MOFs at room temperature.

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Palladium installed copper-organic framework for C–C coupling reactions

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Science China Chemistry 2025年

作者： Yu-Mei Wang Mei-Xia Tao Kai-ming Mo xu Chen Shu-Hua Zhong Elena S Shubina Guo-Hong Ning Dan Li College of Chemistry and Materials Science Guangdong Provincial Key Laboratory of Supramolecular Coordination ChemistryJinan University A.N.Nesmeyanov Institute of Organoelement Compounds Russian Academy of Sciences

The formation of C–C bonds is a crucial aspect of organic synthesis. Synthesists' long-term objective is to identify novel catalysts to optimize reaction systems based on traditional transition metal catalysis. This study explores the synthesis and catalytic potential of palladium-installed covalent metal-organic frameworks(CMOFs) based on copper cyclic trinuclear units(Cu-CTUs). By incorporating palladium ions into the imine-based framework JNM-7 through a straightforward immersion method, we achieved a notable enhancement in catalytic activity for critical C–C coupling reactions, including C–H arylation and Sonogashira cross-coupling. The resulting JNM-7-Pd demonstrated remarkable stability and recyclability, maintaining its efficiency over at least three cycles without any decline in efficiency.

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Pyrococcus ***聚合酶内蛋白子突变体的剪切性质

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山西大学学报(自然科学版) 2000年第2期23卷 149-152页

作者：石亚伟,范俊虎,袁静明,徐明群山西大学生物工程中心!山西太原030006 山西大学生物工程中心!山西太原030006 山西大学生物工程中心!山西太原030006 New England Biolabs!Beverly MAUSA 01915

将含麦芽糖结合蛋白 -嗜热菌 DNA聚合酶内蛋白子基因的 p MI84、p MI94、p MI95三个重组突变体质粒转入 ***2 42 6 ,经发酵及 IPTG低温诱导表达 ,直链淀粉糖亲和纯化获得 MI84(ser1 /Ser5 38Stop)、MI94(Ser1 →Cys H1 /Ser5 38Stop)、M... 详细信息

将含麦芽糖结合蛋白 -嗜热菌 DNA聚合酶内蛋白子基因的 p MI84、p MI94、p MI95三个重组突变体质粒转入 ***2 42 6 ,经发酵及 IPTG低温诱导表达 ,直链淀粉糖亲和纯化获得 MI84(ser1 /Ser5 38Stop)、MI94(Ser1 →Cys H1 /Ser5 38Stop)、MI95 (Ser1 → Ala1 /Ser5 38Stop)三种蛋白质前体。研究了 p H、温度、巯基试剂对内蛋白子 N末端的剪切影响。除 MI95外 ,MI84、MI94前体蛋白均能发生剪切反应 ,DTT能明显促进 MI84、MI94剪切 ,而且 MI94剪切效果优于 MI84。不同还原剂对 MI94诱导差异较小 ,温度升高能明显加快 MI94剪切反应 ,随着 p H升高也能促进 MI94剪切反应。

关键词： MI蛋白前体剪切内蛋白子突变体