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Genome-Wide Association Study of Cooked Rice Textural Attributes and Starch Physicochemical Properties in indica Rice

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Rice science 2024年第3期31卷 300-316,I0018-I0041页

作者： DENG Bowen ZHANG Yanni ZHANG Fan WANG Wensheng xu jianlong ZHANG Yu BAO Jinsong Institute of Nuclear Agricultural Sciences Key Laboratory of Nuclear Agricultural Sciences of Ministry of Agriculture and Zhejiang ProvinceCollege of Agriculture and BiotechnologyZhejiang UniversityHangzhou 310058China Hainan Institute Zhejiang UniversitySanya 572025China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China

Rice cooking and eating qualities(CEQ)are mainly determined by cooked rice textural parameters and starch physicochemical properties.However,the genetic bases of grain texture and starch properties in rice have not been fully understood.We conducted a genome-wide association study for apparent amylose content(AAC),starch pasting viscosities,and cooked rice textural parameters using 279 indica rice accessions from the 3000 Rice Genome Project.We identified 26 QTLs in the whole population and detected single nucleotide polymorphisms(SNPs)with the lowest P-value at the Waxy(Wx)locus for all traits except pasting temperature and cohesiveness.Additionally,we detected significant SNPs at the SUBSTANDARD STARCH GRAIN6(SSG6)locus for AAC,setback(SB),hardness,adhesiveness,chewiness(CHEW),gumminess(GUM),and resilience.We subsequently divided the population using a SNP adjacent to the Waxy locus,and identified 23 QTLs and 12 QTLs in two sub-panels,WxT and WxA,respectively.In these sub-panels,SSG6 was also identified to be associated with pasting parameters,including peak viscosity,hot paste viscosity,cold paste viscosity,and consistency viscosity.Furthermore,a candidate gene encoding monosaccharide transporter 5(OsMST5)was identified to be associated with AAC,breakdown,SB,CHEW,and GUM.In total,39 QTLs were co-localized with known genes or previously reported QTLs.These identified genes and QTLs provide valuable information for genetic manipulation to improve rice CEQ.

关键词： cooking and eating quality genome-wide association study rice Waxy gene

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Amino acids modified nanoscale zero-valent iron:Density functional theory calculations,experimental synthesis and application in the Fenton-like degradation of organic solvents

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Journal of Environmental Sciences 2024年第1期135卷 296-309页

作者： Xingchen Yang Fucheng Ming jianlong Wang Lejin xu Department of Nuclear Engineering and Technology School of Energy and Power EngineeringHuazhong University of Science and TechnologyWuhan 430074China China-EU Institute for Clean and Renewable Energy Huazhong University of Science and TechnologyWuhan 430074China Institute of Nuclear and New Energy Technology Key Laboratory of Advanced Reactor Engineering and Safety of Ministry of EducationTsinghua UniversityBeijing 100084China Beijing Key Laboratory of Radioactive Wastes Treatment Tsinghua UniversityBeijing 100084China

To improve the adsorption and catalytic performance of heterogeneous Fenton-like catalysts for oil wastes,amino acids were used to modify nanoscale zero-valent iron(AA@Fe^(0)),which were applied in the Fenton-like degradation of organic solvents(tributyl phosphate and n-dodecane,named TBP and DD).Twelve amino acids,i.e.,glycine(Gly),alanine(Ala),leucine(Leu),proline(Pro),phenylalanine(Phe),methionine(Met),cysteine(Cys),asparagine(Asn),serine(Ser),glutamic acid(Glu),lysine(Lys)and arginine(Arg),were selected and calculated by density functional theory(DFT).The optimized structure,charge distribution,the highest occupied molecular orbital(HOMO),the lowest unoccupied molecular orbital(LUMO),interaction region indicator(IRI)isosurface map and adsorption energy of AA@Fe^(0),AA@Fe^(0)-TBP and AA@Fe^(0)-DD were studied,which indicated that Fe is more likely to approach and charge transfer with-COO and-NH_(3) on theα-carbon of amino acids.There is strong attraction between Fe and–COO,and Van der Waals force between Fe and-NH_(3),respectively.In the interaction of AA@Fe^(0)with TBP and DD,Van der Waal force plays an important role.AA@Fe^(0)was synthesized in laboratory and characterized to investigate physicochemical properties.In Fenton-like degradation of organic solvents,the change of COD in water phase during the degradation process as well as the volume of the organic phase after the reaction were investigated.The results of calculations combined with experiments showed that Ser-modified Fe^(0)performed the best in these amino acids,with 98%removal of organic solvents.A possible catalytic mechanism was proposed in which amino acids acted a linking role between Fe and organic solvents,activating H_(2)O_(2)to generate hydroxyl radicals for the degradation of organic solvents.

关键词： Amino acids Nanoscale zero-valent iron Density functional theory Organic solvents Fenton-like degradation

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Solubility determination and comparison ofβ-HMX and RDX in two binary mixed solvents(acetonitrile+water,nitric acid+water)

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Chinese Journal of Chemical Engineering 2024年第5期69卷 238-249页

作者： Yuehua Yao Fan Wang Yinguang xu Zishuai xu Lizhen Chen jianlong Wang School of Chemistry and Chemical Engineering North University of ChinaTaiyuan 030051China Gansu Yinguang Chemical Industry Group Co.Ltd. Baiyin 730900China

In order to remove hexahydro-1,3,5-trinitro-1,3,5-triazine(RDX),the main impurity,in process of polymorphic transformation of octrahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine(HMX),the solubility ofβ-HMX and RDX in acetonitrile(ACN)+water in the temperature range of 288.15-333.15 K and in nitric acid(HNO_(3))+water in the temperature range of 298.15-333.15 K were measured by laser dynamic method.The results showed that the solubility of bothβ-HMX and RDX in binary mixed solvents increased monotonously as the temperature increase at a given solvent composition or with increasing of mole fraction of solvent(ACN and nitric acid).Solubility data were well correlated by the modified Apelblat equation,Jouyban-Acree model,Yaws equation and van't Hoff equation,and the Yaws equation achieved the best fitting results according to the relative error and the mean square error root.Furthermore,the solubility ofβ-HMX and RDX in binary mixed solvent was compared,based on the solubility difference and the solvent's own properties,the best separation degree ofβ-HMX and RDX was found when the mole fraction of nitric acid was 0.22 at room temperature,which provided data support for HMX crystallization in mixed solvent.The solubility differences between RDX andβ-HMX in mixed solvents were explained from the formation of intermolecular and intramolecular hydrogen bonds.

关键词： Solubility Octrahydro-1 3 5 7-tetranitro-1 3 5 7-tetrazocine Hexahydro-1 3 5-trinitro-1 3 5-triazine Separation Model Hydrogen bond

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Recent Advances to Enhance Nutritional Quality of Rice

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Rice science 2023年第6期30卷 523-536页

作者： Sundus ZAFAR xu jianlong School of Life Sciences Jiangsu UniversityZhenjiang 212013China Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China The National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

The nutritional quality of rice is a major concern,along with the need to enhance productivity to feed the continuously growing population.Therefore,there is a requirement to breed high-yielding rice varieties with improved nutritional quality that can help combat malnutrition,a global health issue.Undoubtedly,breeding approaches have played a significant role in increasing rice yield while enhancing its nutritional content.In addition to traditional breeding techniques,other recent approaches,such as genetic engineering,gene editing,omics methods,and agronomic practices,must also be employed to meet the nutritional needs of the current population.In this review,we offered detailed information on the development of nutritionally improved rice varieties through the enhancement of protein content,microand macronutrients,vitamins,and oil quality using genetic engineering approaches.We also identified QTLs associated with amino acids,proteins,and micronutrients in rice.Furthermore,omics approaches provide a range of tools and techniques for effectively exploring resources and understanding the molecular mechanisms involved in trait development.Omics branches,including transcriptomics,proteomics,ionomics,and metabolomics,are efficiently utilized for improving rice nutrition.Therefore,by utilizing the information obtained from these techniques and incorporating all of these recent approaches,we can effectively modify the rice genome,directly enhancing the nutritional value of rice varieties.This will help address the challenges of malnutrition in the years to come.

关键词： rice nutritional quality quantitative trait locus genetic engineering omics gene editing

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A novel Effective Panicle Number per Plant 4 haplotype enhances grain yield by coordinating panicle number and grain number in rice

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The Crop Journal 2024年第1期12卷 202-212页

作者： Yun Wang Xiaoqian Wang Laiyuan Zhai Sundus Zafar Congcong Shen Shuangbing Zhu Kai Chen Yun Wang jianlong xu State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China State Key Laboratory of Crop Biology College of AgronomyShandong Agricultural UniversityTai’an 271018ShandongChina Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120GuangdongChina Rice Research Institute Shenyang Agricultural UniversityShenyang 110866LiaoningChina National Nanfan Research Institute(Sanya) Chinese Academy of Agricultural SciencesSanya 572024HainanChina

Increasing effective panicle number per plant(EPN)is one approach to increase yield potential in rice.However,molecular mechanisms underlying EPN remain unclear.In this study,we integrated mapbased cloning and genome-wide association analysis to identify the EPN4 gene,which is allelic to NARROW LEAF1(NAL1).Overexpression lines containing the Teqing allele(TQ)of EPN4 had significantly increased EPN.NIL-EPN4^(TQ) in japonica(geng)cultivar Lemont(LT)exhibited significantly improved EPN but decreased grain number and flag leaf size relative to LT.Haplotype analysis indicated that accessions with EPN4-1 had medium EPN,medium grain number,and medium grain weight,but had the highest grain yield among seven haplotypes,indicating that EPN4-1 is an elite haplotype of EPN4 for positive coordination of the three components of grain yield.Furthermore,accessions carrying the combination of EPN4-1 and haplotype GNP1-6 of GNP1 for grain number per panicle showed higher grain yield than those with other allele combinations.Therefore,pyramiding of EPN4-1 and GNP1-6 could be a preferred approach to obtain high yield potential in breeding.

关键词： Rice Effective panicle number per plant Grain number per panicle Haplotype Grain yield potential

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Saline-Alkali Tolerance in Rice: Physiological Response, Molecular Mechanism, and QTL Identification and Application to Breeding

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Rice science 2022年第5期29卷 412-434页

作者： Ratan Kumar GANAPATI Shahzad Amir NAVEED Sundus ZAFAR WANG Wensheng xu jianlong Institute of Crop Sciences National Key Facility for Crop Gene Resources and Genetic ImprovementChinese Academy of Agricultural SciencesBeijing 100081China Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China

Salinity-alkalinity is incipient abiotic stress that impairs plant growth and development.Rice(Oryza sativa)is a major food crop greatly affected by soil salinity and alkalinity,requiring tolerant varieties in the saline-alkali prone areas.Understanding the molecular and physiological mechanisms of saline-alkali tolerance paves the base for improving saline-alkali tolerance in rice and leads to progress in breeding.This review illustrated the physiological consequences,and molecular mechanisms especially signaling and function of regulating genes for saline-alkali tolerance in rice plants.We also discussed QTLs regarding saline-alkali tolerance accordingly and ways of deployment for improvement.More efforts are needed to identify and utilize the identified QTLs for saline-alkali tolerance in rice.

关键词： saline-alkali tolerance physiological mechanism molecular mechanism marker-assisted backcrossing rice

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Advancements in life-on-a-chip: The impact of “Beyond Limits Manufacturing” technology

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Chinese Chemical Letters 2024年第5期35卷 13-16页

作者： Weiwei He Hongbo Zhang xudong Lin Lili Zhu Tingting Zheng Hao Pei Yang Tian Min Zhang Guoyue Shi Lei Wu jianlong Zhao Gulinuer Wumaier Shengqing Li Yufang xu Honglin Li xuhong Qian Shanghai Key Laboratory of New Drug Design School of PharmacyEast China University of Science and TechnologyShanghai 200237China School of Mechanical and Power Engineering East China University of Science and TechnologyShanghai 200237China Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument School of Biomedical EngineeringShenzhen Campus of Sun Yat-sen UniversityShenzhen 518000China Shanghai Key Laboratory of Green Chemistry and Chemical Processes Department of ChemistrySchool of Chemistry and Molecular EngineeringEast China Normal UniversityShanghai 200241China Shanghai Institute of Microsystem and Information Technology Chinese Academy of SciencesShanghai 200050China Department of Pulmonary and Critical Care Medicine Huashan HospitalFudan UniversityShanghai 200040China Innovation Center for AI and Drug Discovery East China Normal UniversityShanghai 200062China Lingang Laboratory Shanghai 200031China

This review explores the concept of life-on-a-chip,which involves the creation of miniaturized biological systems,such as organs,tissues,and model organisms,on microscale platforms called microfluidic chips.These chips consist of intricately etched channels,wells,and chambers that enable precise control and observation of fluids,cells,and biochemical reactions,facilitating the simulation of various aspects of human or animal physiology and the study of responses to different stimuli,drugs,or disease conditions.The review highlights the application of a novel technology,“Beyond Limit Manufacturing”(BLM),in the development of sophisticated three-dimensional cell models and model organism microchips.Modelorganism-on-a-chip and organ-on-a-chip(OoC)are among the thriving developments in the field of microfluidics,allowing for the reconstruction of living microenvironments and implementation of multiple stimuli.The review discusses the latest advancements in life-on-a-chip technology using BLM and outlines potential future research directions,emphasizing the significant role of these chips in studying complex biological processes in a controlled and scalable manner.

关键词： Beyond Limit Manufacturing Model-organism-on-a-chip Organ-on-a-chip

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液相色谱-串联质谱-内标法测定尿液中河豚毒素

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卫生研究 2024年第1期53卷 95-101页

作者：徐小民蔡增轩许娇娇郑熠斌徐美佳韩见龙浙江省疾病预防控制中心杭州310051

目的建立液相色谱-串联质谱(liquid chromatography-tandem mass spectrometry,LC-MS/MS)-内标法测定尿液中河豚毒素(tetrodotoxin,TTX)的分析方法。方法样品中的TTX经乙酸/甲醇/乙腈(0.005 mL/0.8 mL/1.8 mL)混合溶剂提取,阳离子交换固相萃取(solid phase extraction,SPE)小柱净化,采用含0.3%盐酸的50%乙腈/水洗脱、氨水中和后用XBridgeTMBEH Amide柱(150 mm×3.0 mm,1.7μm)分离,MS/MS测定。通过优化样品提取和SPE净化条件,解决阳离子交换SPE小柱净化尿液基质中TTX回收率偏低、质谱检测基质抑制效应强的难题。结果以春雷霉素为内标定量测定,TTX在0.2~200μg/L范围内线性关系良好,相关系数(r2)0.997,样品基质和纯溶剂中TTX线性相关方程的斜率比为0.95;尿液中TTX检出限和定量限分别为0.1和0.2μg/L;三水平加标(0.2、10.0和200μg/L)平均回收率为89.3%~95.3%,相对标准偏差≤5.1%(n=6)。11例中毒患者尿样中TTX含量为0.4~138μg/L;中毒3天以内获得的尿液中TTX的检出率为100%。结论该方法操作简单、准确、灵敏度高,可以为TTX中毒事件快速处置和毒素体内代谢研究提供可靠的技术支撑。

关键词：液相色谱-串联质谱法固相萃取河豚毒素尿液食物中毒

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应用于远程配电组件系统的改进型CAN总线设计与验证

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电光与控制 2024年第5期31卷 112-117页

作者：陈潇然张之江徐健龙上海大学上海200000 上海飞机设计研究院上海201000

为了将CAN总线应用于国产大飞机的远程配电组件系统,设计了一种改进型的CAN总线应用方案。针对国产大飞机远程配电组件系统的特殊性设计要求,选择合适的网络拓扑结构和通信速率,对CAN的标识符和数据域结构进行合理划分,并定义CAN消息转... 详细信息

为了将CAN总线应用于国产大飞机的远程配电组件系统,设计了一种改进型的CAN总线应用方案。针对国产大飞机远程配电组件系统的特殊性设计要求,选择合适的网络拓扑结构和通信速率,对CAN的标识符和数据域结构进行合理划分,并定义CAN消息转换为ARINC664报文的格式,然后设计附属RPDU和网关RPDU的通信模块。其中,基于ARINC825协议改进了CAN通信的可用性和完整性,基于TTCAN协议改进了ARINC825的通信调度机制,保证了CAN通信的确定性。实验结果证明所提方案满足设计要求,具有可行性。

关键词：远程配电组件系统 CAN总线 ARINC825协议 TTCAN协议

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山地辣椒收获机液压系统的设计与仿真

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机械设计 2024年第1期41卷 94-101页

作者：张建龙唐勇吴荻林蜀云张太华徐卫平贵州师范大学机械与电气工程学院贵州贵阳550025 贵州省山地农业机械研究所贵州贵阳550007

贵州为我国典型的喀斯特地貌省份,辣椒多在坡地种植,现有的辣椒收获机不能满足贵州辣椒采收要求,因此,文中设计并制造了适用于山地地形的全液压自行式履带辣椒收获样机。通过对山地辣椒收获机关键部位液压系统的设计计算及元器件选型,... 详细信息

贵州为我国典型的喀斯特地貌省份,辣椒多在坡地种植,现有的辣椒收获机不能满足贵州辣椒采收要求,因此,文中设计并制造了适用于山地地形的全液压自行式履带辣椒收获样机。通过对山地辣椒收获机关键部位液压系统的设计计算及元器件选型,并采用AMESim液压仿真软件对行走、采收机构的液压系统进行建模与仿真。结果显示,行走马达启动时间为0.52 s,马达流量稳定在117.9 L/min;采摘辊的马达转速稳定在210 r/min,压合辊油缸、地轮油缸和升降油缸的举升用时分别为2.2,3.2和2 s,油缸压力稳定在16 MPa。由此得出,行走系统与采收系统的启动时间短暂且平稳,满足液压系统的设计使用要求。该研究以期为山地辣椒收获机及其他山地机器液压系统的改进与优化设计提供理论依据。

关键词：山地辣椒收获机液压系统计算选型 AMESim仿真

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