检索结果-南通市图书馆

Journal of Integrative Agriculture 2020年第12期19卷 3025-3034页

作者： WEI Dong WANG Zhe xu hui-qian NIU Jin-zhi WANG Jin-jun Chongqing Key Laboratory of Entomology and Pest Control Engineering College of Plant ProtectionSouthwest UniversityChongqing 400715P.R.China State Cultivation Base of Crop Stress Biology for Southern Mountainous Land Academy of Agricultural SciencesSouthwest UniversityChongqing 400715P.R.China

The innate immune system of insects is the front line of self-defense against pathogen invasion. Peptidoglycan recognition proteins (PGRPs) are important components and play key roles in insect immune systems by recognizing peptidoglycan (PGN) in bacterial cell walls. We characterized two isoforms of the PGRP-LC gene, BdPGRP-LCa and BdPGRP-LCb, from Bactrocera dorsalis (Hendel), an important fruit and vegetable pest worldwide. These two isoforms contain an open reading frames of 1 668 bp and 1 731 bp, encoding a protein of 555 and 576 amino acids, respectively. Quantitative real-time PCR results showed that both transcripts were prominently expressed in midgut and fat body of B. dorsalis adult. Inoculation of pathogens showed that both isoforms actively responded to Escherichia coli PGN. We also observed a light response to Staphylococcus aureus PGN. Upon Beauveria bassiana inoculation, the expression of BdPGRP-LCa was enhanced, but the expression of BdPGRP-LCb was suppressed. Suppression of both transcripts by RNA interference led to increased mortality of flies challenged by E. coli, indicating that the two isoforms are involved in sensing Gram-negative bacterial infections.

关键词： oriental fruit fly innate immunity PGRPs Gram-negative bacterium

Remarkable rate acceleration of SmI3-mediated iodination of acetates of Baylis-Hillman adducts in ionic liquid: facile synthesis of (Z)-allyl iodides

在线全文

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2006年第3期7卷 193-197页

作者： LIU Yun-kui ZHENG hui xu Dan-qian xu Zhen-yuan ZHANG Yong-min State Key Laboratory Breeding Base of Green Chemistry-Synthesis Technology Zhejiang University of Technology Hangzhou 310014 China

Stereoselective transformation of Baylis-Hillman acetates I into corresponding （Z）-allyl iodides 2 has been achieved by treatment of I with samarium triiodide in THF. Remarkable rate acceleration of samarium triiodide-mediated iodination of 1 was found when ionic liquid 1-n-butyl-3-methyl-imidazolium tetrafluroborate （[bmim]BF4） was used as reaction media in stead of THF. This novel approach proceeds readily at 50 ℃ within a few minutes to afford （Z）-allyl iodides 2 in excellent yields. A mechanism involving stereoselective iodination of the acetates of Baylis-Hillman adducts by samarium triiodide is described, in which a six-membered ring transition state played a key role in the stereoselective formation of 2.

关键词： Baylis-Hillman adduct (Z)-allyl iodide Samarium triiodide Ionic liquid

在线全文

学校读者我要写书评

暂无评论

芦竹碱法合成DL-色氨酸

应用化学 2006年第6期23卷 668-671页

作者：许前会韦萍淮海工学院化工系连云港222005 南京工业大学制药与生命科学学院

以芦竹碱和海因为原料,经缩合、水解合成DL-色氨酸,收率达41.6%。缩合反应的最佳工艺条件为:反应时间5 h、碘甲烷与芦竹碱的摩尔比为1.2∶1、乙醇钠与芦竹碱的摩尔比为0.8∶1、原料芦竹碱与海因的摩尔比为0.9∶1;水解反应的最佳工艺条件... 详细信息

以芦竹碱和海因为原料,经缩合、水解合成DL-色氨酸,收率达41.6%。缩合反应的最佳工艺条件为:反应时间5 h、碘甲烷与芦竹碱的摩尔比为1.2∶1、乙醇钠与芦竹碱的摩尔比为0.8∶1、原料芦竹碱与海因的摩尔比为0.9∶1;水解反应的最佳工艺条件为:NaOH浓度为1.5 mol/L、水解时间10 h、吲哚甲基海因的浓度为0.2 mol/L、温度为100℃。

关键词： DL-色氨酸芦竹碱吲哚甲基海因合成

Small extracellular vesicles derived from human induced pluripotent stem cell-differentiated neural progenitor cells mitigate retinal ganglion cell degeneration in a mouse model of optic nerve injury

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第2期20卷 587-597页

作者： Tong Li hui-Min Xing Hai-Dong qian Qiao Gao Sheng-Lan xu Hua Ma Zai-Long Chi State Key Laboratory of Ophthalmology Optometry and Visual ScienceEye HospitalWenzhou Medical UniversityWenzhouZhejiang ProvinceChina National Clinical Research Center for Ocular Diseases Eye HospitalWenzhou Medical UniversityWenzhouZhejiang ProvinceChina

Several studies have found that transplantation of neural progenitor cells(NPCs)promotes the survival of injured ***,a poor integration rate and high risk of tumorigenicity after cell transplantation limits their clinical *** extracellular vesicles(sEVs)contain bioactive molecules for neuronal protection and *** studies have shown that stem/progenitor cell-derived sEVs can promote neuronal survival and recovery of neurological function in neurodegenerative eye diseases and other eye *** this study,we intravitreally transplanted sEVs derived from human induced pluripotent stem cells(hiPSCs)and hiPSCs-differentiated NPCs(hiPSC-NPC)in a mouse model of optic nerve *** results show that these intravitreally injected sEVs were ingested by retinal cells,especially those localized in the ganglion cell *** with hiPSC-NPC-derived sEVs mitigated optic nerve crush-induced retinal ganglion cell degeneration,and regulated the retinal microenvironment by inhibiting excessive activation of *** analysis further revealed that hiPSC-NPC derived sEVs transported neuroprotective and anti-inflammatory miRNA cargos to target cells,which had protective effects on RGCs after optic nerve *** findings suggest that sEVs derived from hiPSC-NPC are a promising cell-free therapeutic strategy for optic neuropathy.

关键词： exosome miRNA neural progenitor cell neurodegeneration neuroinflammation neuroprotection optic nerve crush optic neuropathy retinal ganglion cell small extracellular vesicles

The pivotal role of microglia in injury and the prognosis of subarachnoid hemorrhage

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第7期20卷 1829-1848页

作者： Wenjing Ning Shi Lv qian Wang Yuzhen xu Department of Rehabilitation The Second Affiliated Hospital of Shandong First Medical UniversityTaianShandong ProvinceChina Department of Central Laboratory The Affiliated Taian City Central Hospital of Qingdao UniversityTaianShandong ProvinceChina

Subarachnoid hemorrhage leads to a series of pathological changes,including vascular spasm,cellular apoptosis,blood–brain barrier damage,cerebral edema,and white matter ***,which are the key immune cells in the central nervous system,maintain homeostasis in the neural environment,support neurons,mediate apoptosis,participate in immune regulation,and have neuroprotective *** evidence has shown that microglia play a pivotal role in the pathogenesis of subarachnoid hemorrhage and affect the process of injury and the prognosis of subarachnoid ***,microglia play certain neuroprotective roles in the recovery phase of subarachnoid *** approaches aimed at modulating microglia function are believed to attenuate subarachnoid hemorrhage *** provides new targets and ideas for the treatment of subarachnoid ***,an in-depth and comprehensive summary of the role of microglia after subarachnoid hemorrhage is still *** review describes the activation of microglia after subarachnoid hemorrhage and their roles in the pathological processes of vasospasm,neuroinflammation,neuronal apoptosis,blood–brain barrier disruption,cerebral edema,and cerebral white matter *** also discusses the neuroprotective roles of microglia during recovery from subarachnoid hemorrhage and therapeutic advances aimed at modulating microglial function after subarachnoid ***,microglia in subarachnoid hemorrhage are targeted with TLR inhibitors,nuclear factor-κB and STAT3 pathway inhibitors,glycine/tyrosine kinases,NLRP3 signaling pathway inhibitors,Gasdermin D inhibitors,vincristine receptorαreceptor agonists,ferroptosis inhibitors,genetic modification techniques,stem cell therapies,and traditional Chinese ***,most of these are still being evaluated at the laboratory *** clinical studies and data on subarachnoid hemorrhage are required to improve the treatment of subarachnoid hemor

关键词： apoptosis blood–brain barrier brain edema microglia neuroinflammation neuron neuroprotection subarachnoid hemorrhage vasoconstriction white matter injury

Investigating Müller glia reprogramming in mice: a retrospective of the last decade, and a look to the future

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第4期20卷 946-959页

作者： Zhiyuan Yin Jiahui Kang xuan Cheng hui Gao Shujia Huo Haiwei xu Key Lab of Visual Damage and Regeneration&Restoration of Chongqing Southwest Eye HospitalSouthwest HospitalThird Military Medical University(Army Medical University)ChongqingChina

Müller glia,as prominent glial cells within the retina,plays a significant role in maintaining retinal homeostasis in both healthy and diseased *** lower vertebrates like zebrafish,these cells assume responsibility for spontaneous retinal regeneration,wherein endogenous Müller glia undergo proliferation,transform into Müller glia-derived progenitor cells,and subsequently regenerate the entire retina with restored ***,Müller glia in the mouse and human retina exhibit limited neural reprogramming.Müller glia reprogramming is thus a promising strategy for treating neurodegenerative ocular disorders.Müller glia reprogramming in mice has been accomplished with remarkable success,through various *** in molecular,genetic,epigenetic,morphological,and physiological evaluations have made it easier to document and investigate the Müller glia programming process in ***,there remain issues that hinder improving reprogramming efficiency and ***,understanding the reprogramming mechanism is crucial toward exploring factors that will improve Müller glia reprogramming efficiency,and for developing novel Müller glia reprogramming *** review describes recent progress in relatively successful Müller glia reprogramming *** also provides a basis for developing new Müller glia reprogramming strategies in mice,including epigenetic remodeling,metabolic modulation,immune regulation,chemical small-molecules regulation,extracellular matrix remodeling,and cell-cell fusion,to achieve Müller glia reprogramming in mice.

关键词： cell fusion chemical small-molecules epigenetic extracellular matrix immune metabolic mice Müller glia neurodegenerative diseases reprogramming retina regeneration

在线全文

学校读者我要写书评

暂无评论

复合材料粘接结构强度与环境耐久性综述

交通运输工程学报 2021年第6期21卷 78-93页

作者：那景新王广彬庄蔚敏慕文龙徐千卉吉林大学汽车仿真与控制国家重点实验室吉林长春130022 河南农业大学机电工程学院河南郑州450002 吉林大学机械与航空航天工程学院吉林长春130022

为深化对复合材料粘接结构环境耐久性的研究,从胶粘剂基础研究和面向工程的粘接结构应用研究两方面综述了国内外研究现状,探讨了粘接结构老化、疲劳及其耦合作用对强度的影响,总结了单因素和多因素耦合作用下的老化机理,根据基础研究归... 详细信息

为深化对复合材料粘接结构环境耐久性的研究,从胶粘剂基础研究和面向工程的粘接结构应用研究两方面综述了国内外研究现状,探讨了粘接结构老化、疲劳及其耦合作用对强度的影响,总结了单因素和多因素耦合作用下的老化机理,根据基础研究归纳了粘接结构强度预测方法和疲劳寿命预测方法,并对未来研究重点及方向进行展望。分析结果表明:温度和湿度对粘接结构力学性能影响最为显著,多因素耦合作用下的老化更具破坏性,随温度产生的固化收缩、热膨胀系数的差异以及随湿度产生的水解和增塑作用均会使粘接剂老化,且载荷能够加速吸湿对粘接界面造成损伤,从而引发结构过早失效;老化与疲劳之间存在双向耦合作用,随时间变化的交变载荷不仅会影响粘接结构的疲劳寿命,同时还会加速粘接结构老化,而粘接结构在长期服役过程中的老化又会降低结构的疲劳性能;目前尚缺乏对湿热环境与交变载荷耦合作用下老化机理的深入研究,工程应用中的内聚力模型对延展性胶粘剂和厚胶层的预测效果欠佳,应进一步提高内聚力模型在复杂应力状态下的使用精度;损伤力学模型应考虑车辆实际服役工况并加入湿热耦合因素影响以提高使用精度;粘接结构疲劳寿命预测大多基于半经验模型,且对接头疲劳行为的预测局限于特定环境条件;随着粘接技术的进一步发展,对复杂应力状态下粘接结构服役性能的有效评估与建立准静态、疲劳和环境退化综合影响的渐进损伤模型将是未来研究的重点。

关键词：车辆工程粘接结构复合材料老化耦合作用强度设计

Increased excitatory amino acid transporter 2 levels in basolateral amygdala astrocytes mediate chronic stress–induced anxiety-like behavior

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第6期20卷 1721-1734页

作者： Xirong xu Shoumin xuan Shuai Chen Dan Liu qian Xiao Jie Tu Shenzhen Key Laboratory of Neuroimmunomodulation for Neurological Diseases Shenzhen-Hong Kong Institute of Brain ScienceShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina CAS Key Laboratory of Brain Connectome and Manipulation Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina Guangdong Provincial Key Laboratory of Brain Connectome and Behavior the Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina University of Chinese of Academy of Sciences BeijingChina Faculty of Life and Health Sciences Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina

The conventional perception of astrocytes as mere supportive cells within the brain has recently been called into question by empirical evidence, which has revealed their active involvement in regulating brain function and encoding behaviors associated with ***, astrocytes in the basolateral amygdala have been found to play a role in the modulation of anxiety-like behaviors triggered by chronic stress. Nevertheless, the precise molecular mechanisms by which basolateral amygdala astrocytes regulate chronic stress–induced anxiety-like behaviors remain to be fully elucidated. In this study, we found that in a mouse model of anxiety triggered by unpredictable chronic mild stress, the expression of excitatory amino acid transporter 2 was upregulated in the basolateral amygdala. Interestingly, our findings indicate that the targeted knockdown of excitatory amino acid transporter 2 specifically within the basolateral amygdala astrocytes was able to rescue the anxiety-like behavior in mice subjected to stress. Furthermore, we found that the overexpression of excitatory amino acid transporter 2 in the basolateral amygdala, whether achieved through intracranial administration of excitatory amino acid transporter 2agonists or through injection of excitatory amino acid transporter 2-overexpressing viruses with GfaABC1D promoters, evoked anxiety-like behavior in mice. Our single-nucleus RNA sequencing analysis further confirmed that chronic stress induced an upregulation of excitatory amino acid transporter 2 specifically in astrocytes in the basolateral amygdala. Moreover, through in vivo calcium signal recordings, we found that the frequency of calcium activity in the basolateral amygdala of mice subjected to chronic stress was higher compared with normal *** knocking down the expression of excitatory amino acid transporter 2 in the basolateral amygdala, the frequency of calcium activity was not significantly increased, and anxiety-like behavior was ob

关键词： anxiety astrocytes basolateral amygdala behavior dihydrokainic acid excitatory amino acid transporter 2 fiber photometry glutamate LDN-212320 transporter

在线全文

学校读者我要写书评

暂无评论

艾灸对力竭运动心肌细胞保护的研究进展

针刺研究 2014年第6期39卷 504-507页

作者：胡引顾一煌张宏如许慧倩南京中医药大学第二临床医学院南京210046

艾灸对消除运动疲劳,预防和保护心肌损伤的作用机制是当今运动医学界十分关注的研究方向。笔者查阅了近10年来艾灸防治运动性心肌损伤的相关文献后对其作用机制进行综述,发现艾灸主要从调节心肌细胞内分泌功能,清除自由基,抗氧化损伤,... 详细信息

艾灸对消除运动疲劳,预防和保护心肌损伤的作用机制是当今运动医学界十分关注的研究方向。笔者查阅了近10年来艾灸防治运动性心肌损伤的相关文献后对其作用机制进行综述,发现艾灸主要从调节心肌细胞内分泌功能,清除自由基,抗氧化损伤,影响心肌细胞血液流变性几个方面发挥对力竭运动心肌细胞的保护作用,在此基础上,从分子水平提出艾灸对力竭运动心肌细胞保护的可能新机制——端粒酶调控机制。

关键词：艾灸疗法力竭运动心肌损伤线粒体端粒酶

在线全文

学校读者我要写书评

暂无评论

结点局部能量损失对管网水力计算的影响

水利水电技术 2007年第1期38卷 78-81,85页

作者：茅泽育徐千惠孙梅罗昇清华大学水利水电工程系北京100084 清华大学环境科学与工程系北京100084

对于管径较大、管段长度较短的管网,结点处的动量交换和局部能量损失显著影响管网中管段通过流量的计算结果。进行管网水力计算时,忽略结点处的局部能量损失或采用不变的局部能量损失系数均是不合适的。文中分别应用三种计算方法,即忽... 详细信息

对于管径较大、管段长度较短的管网,结点处的动量交换和局部能量损失显著影响管网中管段通过流量的计算结果。进行管网水力计算时,忽略结点处的局部能量损失或采用不变的局部能量损失系数均是不合适的。文中分别应用三种计算方法,即忽略结点处局部能量损失、采用恒定不变的结点局部能量损失系数,以及根据试验成果所得的与水流条件有关的半经验公式,针对短管段构成的管网系统进行了计算比较,管段流量的计算结果相差很大。研究结果还表明,与同一结点相接的各管段间存在显著的能量交换,因此,忽略或采用恒定不变的能量损失系数均不能正确地反映这些水流现象。

关键词：管网水力计算局部能量损失 T形结点分流汇流