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Journal of Integrative Agriculture 2021年第8期20卷 2195-2203页

作者： YANG Ya-jun xu hong-xing WU Zhi-hong LU Zhong-xian State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Institute of Plant Protection and MicrobiologyZhejiang Academy of Agricultural SciencesHangzhou 310021P.R.China

Midgut juice plays an important role in food digestion and detoxification in *** order to understand the potential of midgut juice of Cnaphalocrocis medinalis(Guenée)to degrade Bt proteins,the enzymatic activity of midgut juice and its degradation of Bt proteins(Cry2A,Cry1C,Cry1Aa,and Cry1Ac)were evaluated in this study through protease inhibitor *** activities of total protease in midgut juices were significantly inhibited by phenylmethylsulfonyl fluoride(PMSF),tosyl-L-lysine chloromethyl ketone(TLCK),pepstatin A and *** enzymatic activity of chymotrypsin was significantly inhibited by PMSF,and enzymatic activity of trypsin was significantly inhibited by ethylenediaminetetraacetic acid(EDTA),PMSF,tosyl phenylalanine chloromethyl ketone(TPCK),TLCK and trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane(E-64).EDTA could significantly inhibit the degradation of Cry2A by ***,PMSF,TPCK,and TLCK could inhibit the degradation of Cry1C and ***,PMSF,TPCK,TLCK,and E-64 could inhibit the degradation of *** results indicated that some protease inhibitors hindered various enzymatic activities in the larval midgut of ***,which may reduce the insect’s ability to degrade Bt *** findings may aid the application of protease inhibitors in the management of this insect pest in the future.

关键词： Cnaphalocrocis medinalis midgut juice protease inhibitor enzyme activity degradation Bt protein

pH influences the profiles of midgut extracts in Cnaphalocrocis medinalis(Guenée) and its degradation of activated Cry toxins

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Journal of Integrative Agriculture 2020年第3期19卷 775-784页

Midgut extracts play crucial roles in food digestion and *** evaluated the effect of pH on the profiles of the midgut extracts in rice leaffolder,Cnaphalocrocis medinalis and the degradation of activated Bt-toxins by the midgut extracts under different pH *** protease activity increased slightly with the increase with the simulated pH in the midgut extracts and the maximal protease activity was observed at pH 10.5,while an upward trend was observed as the pH of reaction buffer *** of chymotrypsin-like enzymes increased with pH,both in the buffer and midgut extracts,while the activity of trypsin-like enzyme was *** of the activated Cry2 A by the midgut extracts enhanced as the pH ***2 A was fully degraded into smaller segments at pH *** Cry1 C protein at pH 9.0-10.5 was partially degraded by the midgut *** Cry1 Aa and Cry1 Ac were partially degraded into fragments by the midgut extracts at high *** results will facilitate our further understanding of the interactions between *** and the Cry toxin.

关键词： Cnaphalocrocis medinalis Bacillus thuringiensis pH digestion degradation protease

Controllable Excitation of Surface Plasmons in End-to-Trunk Coupled Silver Nanowire Structures

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Chinese Physics Letters 2012年第7期29卷 221-225页

作者： ZHU Yin WEI hong YANG Peng-Fei xu hong-xing Beijing National Laboratory for Condensed Matter Physics and Institute of Physics Chinese Academy of SciencesP.O.Box 603-146Beijing 100190

In branched nanowire structures,the controllable excitation of surface plasmons is investigated by both experi-ments and *** focusing the excitation light at the junction between the main wire and the branch wire,surface plasmons can be selectively launched to propagate to different output terminals,depending on the polarization of the excitation *** parameters influencing the plnsmon excitation and thus emission behavior are investigated,including the branch angle,the position of the branch and the nanowire *** different polarization dependence of the output light is determined by the surface plasmon modes selectively excited in the junction through end-excitation or/and gap-excitation *** the branch wire,when the branch angle is small,the end-excitation is dominant,which makes the branched wire behave like an individual *** the increase of the branch angle,the coupling between the branch wire end and the primary wire trunk is increased,which influences the plasmon excitation in the branch wire as evidenced by the rotation of the polarization angle for maximum *** the primary wire,the SP excitation is dependent on the branch angle,position of the junction along the primary wire,and the radii of the *** results may be important for the design of a controllable surface plasmon launcher,one of the functional components in surface-plasmon-based nanophotonic circuits.

关键词： excitation branch rotation

A Facile Method for Synthesizing TiO_(2) Sea-Urchin-Like Structures and Their Applications in Solar Energy Harvesting

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Chinese Physics Letters 2011年第7期28卷 301-304页

作者： WANG Wen-Hui WANG Wen-Zhong xu hong-xing Beijing National Laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of SciencesBeijing 100190 College of Science Minzu University of ChinaBeijing 100081

We present a new method to prepare TiO_(2) sea−urchin-like structures,which involves the initial formation of tubular nanostructures and subsequent self-assembly of the nanotubes into micrometer-scale sea-urchin-like *** also investigate the important role of alkali aqueous conditions in the preparation of TiO_(2) sea−urchin-like *** facile and cost-effective approach provides a new route for the preparation of self-assembled TiO_(2) *** addition,the performance of the as−synthesized TiO2 sea-urchin-like structures as the active layer of an efficient solar energy harvester is also studied and discussed.

关键词： alkali subsequent preparation

Influence of Temperature on Symbiotic Bacterium Composition in Successive Generations of Egg Parasitoid, Anagrus nilaparvatae

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Rice science 2016年第4期23卷 203-210页

作者： WANG Xin xu hong-xing LIU Shu-ping TANG Jiang-wu ZHENG xu-song LV Zhong-xian State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control Institute of Plant Protection and Microbiology Zhejiang Academy of Agricultural Sciences

Anagrus nilaparvatae is the dominant egg parasitoid of rice planthoppers and plays an important role in biological control. Symbiotic bacteria can significantly influence the development, survival, reproduction and population differentiation of their hosts. To study the influence of temperature on symbiotic bacterial composition in the successive generations of A. nilaparvatae, A. nilaparvatae were raised under different constant temperatures of 22 °C, 25 °C, 28 °C, 31 °C and 34 °C. Polymerase chain reaction-denaturing gradient gel electrophoresis was used to investigate the diversity of symbiotic bacteria. Our results revealed that the endophytic bacteria of A. nilaparvatae were Pantoea sp., Pseudomonas sp. and some uncultured bacteria. The bacterial community composition in A. nilaparvatae significantly varied among different temperatures and generations, which might be partially caused by temperature, feeding behavior and the physical changes of hosts. However, the analysis of wsp gene showed that the Wolbachia in A. nilaparvatae belonged to group A, sub-group Mors and sub-group Dro. Sub-group Mors was absolutely dominant, and this Wolbachia composition remained stable in different temperatures and generations, except for the 3rd generation under 34 °C during which sub-group Dro became the dominant Wolbachia. The above results suggest that the continuous high temperature of 34 °C can influence the Wolbachia community composition in A. nilaparvatae.

关键词： temperature Anagrus nilaparvatae Wolbachia bacterial community polymerase chain reaction-denaturing gradient gel electrophoresis rice brown planthopper

Sustainable Management of Rice Insect Pests by Non-Chemical-Insecticide Technologies in China

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Rice science 2017年第2期24卷 61-72页

作者： xu hong-xing YANG Ya-jun LU Yan-hui ZHENG xu-song TIAN Jun-ce LAI Feng-xiang FU Qiang LU Zhong-xian Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control Institute of Plant Protection and MicrobiologyZhejiang Academy of Agricultural SciencesHangzhou 310021China China National Rice Research Institute Hangzhou 310006China

Chemical pesticides play crucial roles in the management of crop diseases and pests. However, excessive and irrational use of pesticides has become a major concern and obstacle to sustainable agriculture. As a result, the quality and security of agricultural products are reduced, and the ecological and environmental integrities are threatened. Recently, environment-friendly pest management measures have been introduced and adopted to manage rice insect pests and reduce the use of insecticides. This paper reviewed the advancements in development and application of non-chemical technologies for insect pest management during rice production in China.

关键词： rice chemical insecticide reduce insecticide non-chemical pest management ecological and environmental integrity

Electrophysiological responses of Plutella xylostella to 9 different plant volatiles

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环境昆虫学报 2011年第4期33卷 442-447页

作者： xu hong-xing WANG Su TAN Xiao-ling ZHANG Fan Lab of Natural Enemy Research Institute of Plant&Environment ProtectionBeijing Academy of Agriculture&Forestry SciencesBeijing 100097China College of Plant Protection Northwest A&F UniversityYangling 712100Shannxi ProvinceChina

Plutella xylostella is the main pest of cruciferae plants in the worldwide *** volatiles released from host plants serve vital roles in host-finding and oviposition *** were recorded from male and female Plutella xylostella to 9 different plant volatiles(cabbage Brassica oleracea ***,baby bokchoi Brassica chinensis L.,broccoli Brassica oleracea *** L.,Chinese cabbage Brassica pekinensis Rupr.,Radish Raphanus sativus L.,towel gourd Luffa Cylindrica Roem.,eggplant Solanum melongena L.,tomato Solanum lycopersicum,pepper Capsicum annuum L.) in healthy/injured *** statistic analysis show there is a significant difference of EAG relative values between cruciferae and non-cruciferae *** EAG relative values of injured plant volatiles to both male and female changed a lot during the ***-ANOVA analysis represents cross factors(sex,host-plant,plant status) showed a great interactive impacts to the EAG-RV.

关键词： cruciferae EAG Plutella xylostella plant odours volatiles collention host specificity

High Temperature Modifies Resistance Performances of Rice Varieties to Brown Planthopper,Nilaparvata lugens(Stl)

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Rice science 2010年第4期17卷 334-338页

作者： WANG Bao-ju xu hong-xing ZHENG xu-song Fu Qiang Lu Zhong-xian Institute of Plant Protection and Microbiology Zhejiang Academy of Agricultural Sciences Itangzhou 310021 China School of Life and Environment Science Hangzhou Normal University Hangzhou 310036 China China National Rice Research Institute Hangzhou 310006 China

To investigate the effect of temperature on the resistance characteristics of dce varieties with different resistance genes to brown planthopper （BPH）, Nilaparvata lugens （Stal）, the resistances of IR26 （Bphl） and IR36 （bph2） to BPH population in Hangzhou, China were monitored in greenhouse during September in 2007 and 2008 by using the standard seedling screening techniques （SSST） developed by the International Rice Research Institute （IRRI）. Furthermore, the changes in resistance of IR26 and IR36 to BPH, soluble sugar and oxalic acid contents in 25-day-old rice plants of susceptible variety TN1 and resistant varieties IR26 and IR36 were detected at five temperatures （22℃, 25℃, 28℃, 31℃ and 34℃）. IR26 completely lost resistance both in greenhouse and at the five tested temperatures. IR36 still had moderate resistance at natural temperature, but its resistance decreased gradually when the temperature increased from 25℃ to 34℃, and fully lost its resistance at 31℃ and 34℃. The highest durable resistance of IR26 and IR36 were recorded at 25℃. The soluble sugar content in plants of the three tested rice varieties increased with temperature increase, and the oxalic acid content increased with the temperature increase at first, maximized at 25℃, and then declined. Two-way ANOVA indicated significant effects of temperature and rice variety on contents of soluble sugar and oxalic acid in rice plants

关键词： brown planthopper Nilaparvata lugens temperature rice resistant characteristics

Bacterial Community in Different Populations of Rice Brown Planthopper Nilaparvata lugens(Stl)

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Rice science 2014年第1期21卷 59-64页

作者： xu hong-xing ZHENG xu-song YANG Ya-jun WANG Xin YE Gong-yin LU Zhong-xian Institute of Insect Science Zhejiang University State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control Institute of Plant Protection and Microbiology Zhejiang Academy of Agricultural Sciences

The structures of bacterial communities in the brown planthopper （BPH） Nilaparvata lugens （St^l） from different geographic and resistant virulent populations were analyzed by using denatured gradient gel electrophoresis （DGGE）. Results showed that the bacterial communities in BPH nymph from the first to the fifth instars varied with nymphal growth and development. The bacterial communities in the first-instar BPH nymph were similar to those in adults. Nine geographic BPH populations were divided into three groups based on the cluster analysis of DGGE fingerprint. The first group was from the Philippines; the second group was from Thailand and Hainan, Yunnan and Zhejiang provinces of China; and the third group was from Vietnam and Guangxi, Hunan and Jiangxi provinces of China. BPH populations adapted to different resistant rice varieties. The BPH populations from Mudgo （with resistant gene Bphl） and ASD7 （with resistant gene bph2） differed with those of the susceptible rice variety TNI.

关键词： brown planthopper endosymbiont bacterial community geographic population virulence resistant variety

What is the Role of Gravity,Turbulence and Magnetic Fields in High-mass Star Formation Clouds?

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Research in Astronomy and Astrophysics 2024年第6期24卷 21-27页

作者： An-xu Luo hong-Li Liu Guang-xing Li Sirong Pan Dong-Ting Yang School of Physics and Astronomy Yunnan UniversityKunming 650091China South-Western Institute for Astronomy Research Yunnan UniversityKunming 650500China

To explore the potential role of gravity,turbulence and magnetic fields in high-mass star formation in molecular clouds,this study revisits the velocity dispersion–size(σ–L)and density–size(ρ–L)scalings and the associated turbulent energy spectrum using an extensive data *** sample includes various hierarchical density structures in high-mass star formation clouds,across scales of 0.01–100 *** observeσ∝L^(0.26)andρ∝L^(-1.54)scalings,converging toward a virial equilibrium state.A nearly flat virial parameter–mass(α_(vir)-M)distribution is seen across all density scales,withα_(vir)values centered around unity,suggesting a global equilibrium maintained by the interplay between gravity and turbulence across multiple *** turbulent energy spectrum(E(k))analysis,based on theσ–L andρ–L scalings,yields a characteristic E(k)∝k^(-1.52).These findings indicate the potential significance of gravity,turbulence,and possibly magnetic fields in regulating dynamics of molecular clouds and high-mass star formation therein.

关键词： ISM:clouds stars:formation stars:kinematics and dynamics