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Relationship between Acute Organophosphorus Pesticide Poisoning and Damages Induced by Free Radicals

Biomedical and Environmental Sciences 2002年第2期15卷 177-186页

作者： ZHOU JUN-FU xu gen-bo FANG WEI-JUN SecondAffiliatedHospitalofCollegeofMedicine ZhejiangUniversity88JiefangRoadHangzhou310009ZhejiangProvinceChina

To study the relationship between abnormal reactions of free radicals in bodies of patients with acute organophosphorus pesticide poisoning (AOPP) and damages induced by free *** 58 AOPP patients and 58 healthy adult volunteers(HAV)were enrolled in an independent samples control design,in which spectrophotometric methods were used to determine the concentrations of nitric oxide(NO)and lipoperoxides(LPO)in plasma,and LPO in erythrocytes,vitamin C (VC),vitamin E(VE)and β-carotene (β-CAR)in plasma as well as activities of superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-Px)and acetylcholiesterase(AChE) in ***:Compared with the average values of every biochemical parameter in the HAV group,the average values of LPO in plasma and in erythrocytes,and NO in plasma in the AOPP group were significantly increased(P=0.00001),while the average values of VC,VE,β-CAR in plasma as well as SOD,CAT,GSH-PX and AChE in erythrocytes in the AOPP group were significantly decreased(P=0.000001).The finding of Pearoson product-moment correlation analysis between the value of AChE in erythrocytes and the values of above biochemical parameters for 58 AOPP patients showed that there was a significant linear negative correlation between AChE in erythrocytes and LPO,NO in plasma,and LPO in erythrocytes(P=0.00001-0.001319),while there was a significant linear positve correlation between AChE in erythrocytes and VC,VE,β-CAR in plasma as well as SOD,CAT,GSH-Px in erythrocytes(P=0.000013-0.000824),The results of discriminant analysis of above chemical parameters for 58 AOPP patients and 58 HAV suggested that the correct rates of discriminant analysis were increased to 100 3.395193e-313n the values of AChE and LPO in plasma and in erythrocytes,or AChE and others,were jointly used for the discriminant analysis ,Conclusion:The findings of the present study suggest that a series of free radical reactions in AOPP patients’ bodies are pathologically aggravat

关键词： Organophosphorus pesticide poisoning Free radicals Lipoperoxides Nitric oxide Antioxidants Antioxidases

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Novel distribution pattern of fibrinolytic components in rabbit tissues extract: a preliminary study

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第8期8卷 570-574页

作者： LU Xing-guo WU Xian-guo xu Xiao-hua GONG xu-bo ZHOU xuan xu gen-bo ZHU Lei ZHAO Xiao-ying Department of Haematology the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China Department of Clinical Laboratory Diagnosis the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China

Objective: The purpose of this work was to investigate the distribution pattern of fibrinolytic factors and their inhibitors in rabbit tissues. Methods: The components of the fibrinolytic system in extracts from a variety of rabbit tissues, including tissue plasminogen activator (tPA), plasminogen activator inhibitor-1 (PAI-1), plasminogen (Plg), plasmin (Pl) and α2 plasmin inhibitor (α2PI), were determined by colorimetric assay. Results: The tissue extracts in renal, small intestine, lung, brain and spleen demonstrated strong fibrinolytic function, in which high activity of tPA, Plg and Pl was manifested; whereas in skeletal muscle, tongue and stomach, higher activity of PAI-1 and α2PI showed obviously. Also excellent linear correlations were found between levels of tPA and PAI-1, Pl and α2PI, Plg and Pl. In related tissues, renal cortex and renal marrow showed distinctly higher activity of tPA and lower activity of PAI-1, with the levels of Plg and Pl in renal cortex being higher than those in renal marrow, where the α2PI level was higher than that in renal cortex. Similarly, the levels of tPA, Plg and Pl in small intestine were higher than those in large intestine, but with respect to PAI-1 and α2PI, the matter was reverse. In addition, the fibrinolytic activity in muscle tissue was lower, however, the levels of tPA, Plg, and Pl in cardiac muscle were obviously higher than those in skeletal muscles, and the levels of PAI-1 and α2PI were significantly lower than those in skeletal muscle. Conclusion: Our data demonstrate that a remarkable difference of the fibrinolytic patterns exists in rabbit tissues, which has probable profound significance in understanding the relationship between the function of haemostasis or thrombosis and the physiologic function in tissues.

关键词： Tissue extract Fibrinolytic factors Fibrinolytic inhibitors

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Technique for Roof-turf Establishment and management

Technique for Roof-turf Establishment and management

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中国草学会草坪专业委员会第六届全国会员代表大会及第九次学术研讨会

作者： xu Li-gen,QIN bo-cai, LI xue-qing, AND MA bo-ying (College of Life Sciences, Zhejiang University, Hangzhou, 310012, China Zhejiang Educational College, Hangzhou 310012,China)

This article is a summary written by the authors according to their researches result in the past decade on the roof lawn in the areas of its history, its the key technologies in grow and maintenance, economic index, ecosystem effect, etc. It also provides the evaluation on the accountability and feasibility of the application of technology.

关键词： Roof lawn Roof turf Establishment Management

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4-氯苯甲酰小檗胺体内外抑制伊马替尼耐药K562细胞增殖的作用及机制

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南方医科大学学报 2011年第12期31卷 1997-2001页

作者：张云锋徐根波甘宜超许晓华徐荣臻浙江大学医学院附属第二医院血液科浙江杭州310009

目的研究4-氯苯甲酰小檗胺(BBD9)体内外抑制伊马替尼(IM)耐药k562细胞(K562/IR)的增值作用,并探讨其机制。方法 MTT法测定BBD9和小檗胺(BBM)IC50以比较药效。0.5μg/ml BBD9或8μg/ml BBM处理体外培养K562/IR细胞48 h Western blot检测p... 详细信息

目的研究4-氯苯甲酰小檗胺(BBD9)体内外抑制伊马替尼(IM)耐药k562细胞(K562/IR)的增值作用,并探讨其机制。方法 MTT法测定BBD9和小檗胺(BBM)IC50以比较药效。0.5μg/ml BBD9或8μg/ml BBM处理体外培养K562/IR细胞48 h Western blot检测p210Bcr-Abl,IKKα,胞浆和胞核NF-κBp65表达水平。不同浓度BBD9处理体外培养的K562/IR48 h后流式测定细胞存活,凋亡,坏死比例;Western blotting检测PARP、Caspase3、Caspase9和LC3II表达水平。裸鼠移植瘤模型组1(15 mg/kg BBD9)、组2(30 mg/kg BBD9)、组3(100 mg/kg IM)和对照组(不给药)中移植瘤的瘤质量、瘤消退率和裸鼠体质量检测。结果 BBD9和BBM IC50分别为0.73μg/ml和5.43μg/ml。0.5μg/ml BBD9或8μg/ml BBM处理48 h后p210Bcr-Abl,IKKα和胞核NF-κBp65表达均下降且以BBD9更明显(P<0.05),胞浆NF-κBp65基本一致。细胞凋亡,坏死均和BBD9呈计量依赖;活化的PARP,Caspase3,Caspase9和LC3 II也随着药物浓度增加而升高(P<0.05)。裸鼠移植瘤瘤质量、瘤消退率、和体质量等指标BBD9给药组优于IM给药组(P<0.05)。结论 BBD9对K562/IR体内外均能起效,抑制p210Bcr-Abl,IKKα表达和胞浆NF-κBp65向胞核转位,并激活凋亡,坏死,自噬等多条死亡通路。

关键词： 4-氯苯甲酰小檗胺伊马替尼耐药 K562细胞

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骨髓印片在骨髓细胞学检验中的应用价值

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中华医学杂志 2010年第22期90卷 1531-1536页

作者：龚旭波卢兴国徐根波吴先国王琳张晓红朱蕾王炜琴浙江大学医学院附属第二医院血液科骨髓实验室杭州310009

目的研究骨髓印片在血液病诊断中的应用价值.方法收集2002年1月至2008年6月同步骨髓涂片、印片和切片检查的3024例初诊血液系统疾病患者（男1667例,女1357例,年龄7～82岁,中位年龄55岁）的骨髓标本重新阅片,以切片有核细胞量为标准评... 详细信息

目的研究骨髓印片在血液病诊断中的应用价值.方法收集2002年1月至2008年6月同步骨髓涂片、印片和切片检查的3024例初诊血液系统疾病患者（男1667例,女1357例,年龄7～82岁,中位年龄55岁）的骨髓标本重新阅片,以切片有核细胞量为标准评估印片与涂片的指标性能,并以临床汇总诊断为依据,比较骨髓印片、涂片和切片的诊断符合率.选取79例浸润性淋巴瘤、114例浆细胞骨髓瘤（PCM）患者骨髓标本,对肿瘤细胞在印片与切片中的浸润方式进行等级相关分析.结果骨髓印片含较明显的造血区和非造血区,细胞基本保留了完整的形态学结构.骨髓印片的有核细胞量评估性能优于涂片（P〈0.05）而与切片相近（P〉0.05）.印片对脾功能亢进（130例）、骨髓转移癌（67例）、伴原始细胞增多难治性贫血（27例）、骨髓增殖性肿瘤（174例）和PCM（94例）的诊断符合率高于涂片（分别为96.9%比80.7%、91.0%比76.1%、92.6%比81.5%、92.5%比76.4%、97.8%比92.6%,均P〈0.05）,对巨幼红细胞性贫血（69例）、急性髓细胞白血病亚型（104例）、单系和多系增生异常难治性血细胞减少（分别为15例和22例）、淋巴浆细胞性淋巴瘤（12例）的诊断符合率高于切片（分别为100%比84.0%,91.3%比74.0%,86.7%比60.0%,90.9%比72.7%,66.6%比50.0%,均P〈0.05）,对骨髓增生异常-骨髓增殖性肿瘤（26例）的诊断符合率（96.2%）高于涂片（76.3%,P〈0.05）和切片（78.2%,P〈0.05）.浸润性淋巴瘤与PCM患者骨髓印片和切片中肿瘤细胞浸润方式具有良好的相关性（r=0.90,r=0.78,均P〈0.05）.结论骨髓印片兼有涂片和切片的特征.印片评估有核细胞量优于涂片,也可预测切片中肿瘤细胞浸润的某些方式.印片单个核细胞的形态学清晰度优于骨髓切片,对主要依据形态学进行的诊断具有明显优势.

关键词：骨髓检查诊断血液病

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骨髓间质干细胞及其临床应用的研究进展

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中国肿瘤 2005年第11期14卷 740-743页

作者：王珍徐根波浙江大学医学院附属第二医院浙江杭州310009

骨髓间质干细胞(mesenchymalstemcell,MSC)是骨髓内除造血干细胞之外的另一重要成分,除具有自我更新和多向分化的潜能外,更具有很强的可塑性。近年来很多研究发现骨髓间质干细胞在适宜的体外培养中能生存、增殖,并保存多向分化潜能。因... 详细信息

骨髓间质干细胞(mesenchymalstemcell,MSC)是骨髓内除造血干细胞之外的另一重要成分,除具有自我更新和多向分化的潜能外,更具有很强的可塑性。近年来很多研究发现骨髓间质干细胞在适宜的体外培养中能生存、增殖,并保存多向分化潜能。因其移植免疫排斥反应弱,使得骨髓间质干细胞成为组织工程中重要的替代细胞。作为基因的一个载体,其在基因治疗方面也表现出较好的前景。文章就间质干细胞的生物学特性和临床应用前景做一综述。

关键词：间质干细胞生物学特性组织工程基因治疗

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恶性血液病化疗患者院内下呼吸道感染的随访观察

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中华预防医学杂志 2008年第2期42卷 123-126页

作者：陆群黄连生张嵘徐根波赵小英浙江大学医学院附属第二医院医院感染管理科杭州 310009

目的随访观察恶性血液病化疗患者下呼吸道感染的危险因素和病原菌变迁。方法对2004年4月—2005年12月的50例各种类型恶性血液病拟准备化疗的患者进行化疗前和化疗后不同时间段的呼吸道菌群定量分析，并对院内感染的细菌进行药敏试验，... 详细信息

目的随访观察恶性血液病化疗患者下呼吸道感染的危险因素和病原菌变迁。方法对2004年4月—2005年12月的50例各种类型恶性血液病拟准备化疗的患者进行化疗前和化疗后不同时间段的呼吸道菌群定量分析，并对院内感染的细菌进行药敏试验，对院内感染的同一种类的细菌用脉冲场凝胶电泳（PFGE）进行同源性分析。结果恶性血液病患者化疗后下呼吸道感染的发生率为16％。鲍曼不动杆菌、大肠埃希菌和真菌是主要的院内感染致病菌。其中不动杆菌对头孢菌素类、喹诺酮类、氨基糖甙类、碳青霉烯类和含酶抑制剂类抗生素高度耐药，但对头孢哌酮和（或）舒巴坦这一含酶抑制剂类抗生素较为敏感。用PFGE同源性分析结果为2个克隆株。结论恶性血液病化疗患者院内下呼吸道感染的随访观察可以为抗生素的合理使用和预防、控制院内感染的发生提供理论依据。

关键词：血液肿瘤呼吸道感染药物疗法因素分析统计学

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Targets capture by distributed active swarms via bio-inspired reinforcement learning

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 210-221页

作者： Kun xu Yue Li Jun Sun Shuyuan Du Xinpeng Di Yuguang Yang bo Li Institute of Biomechanics and Medical Engineering Applied Mechanics Laboratory Department of Engineering MechanicsTsinghua University Institute of Nuclear and New Energy Technology Tsinghua University Shanghai Aerospace Control Technology Institute Shanghai Key Laboratory of Aerospace Intelligent Control Technology College of Automation Engineering Nanjing University of Aeronautics and Astronautics

Natural swarms arranged from cells to herds are usually decentralized but display intriguing collective intelligence in coordinating individuals across large scales to efficiently achieve their common goals. Learning from nature may provide new strategies for controlling collective dynamics of synthetic swarms to accomplish specific functions. Here, we present a bioinspired computational framework that steers distributed active swarms to collectively capture and merge targets via reinforcement learning. We exploit collective milling structures of natural herds to cage the targets, and adopt a switching control policy inspired by sperms' chiral dynamics to optimize the trajectories of individuals, through which the active swarms can selforganize to enclose single or multiple distant targets in a dynamical, adaptive and scalable manner. There exists a critical swarm size, beyond which the excessive competition between agents would generate large mechanical forces, leading to capture instability but enabling the transition from short-distance to long-distance merging capture of multiple targets. This work provides physical insights into distributed active swarms and could offer a multilevel, decentralized strategy toward controlling swarm robotics in wide applications such as bio-medical devices, machine immunity, and target clearance.

关键词： swarm intelligence robotics multi-tasks artificial intelligence bionics

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Construction of a genetic linkage map and mapping of a female-specific DNA marker in half-smooth tongue sole（Cynoglossus semilaevis）

Construction of a genetic linkage map and mapping of a femal...

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2009年中国水产学会学术年会

作者： LIAO Xiao-Lin,MA Hong-Yu,xu gen-bo,SHAO Chang-Wei,TIAN Yong-Sheng, JI Xiang-Shan,YANG Jing-Feng,CHEN Song-Lin Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China

＜正＞The half-smooth tongue sole（Cynoglossus semilaevis,hereafter,‘tongue sole’） is a marine flatfish with great commercial importance for fisheries and aquaculture in *** has also been a promising model for the study of sex determination mechanisms in ***,we report the construction of a genetic linkage map for the tongue sole,based on 137 markers including 103 AFLP markers,33 microsatellite markers,and one female-specific DNA ***-six linkage groups（LGs） were found. The total map length was 934.6 cM（Kosambi）,with an average spacing of 8.4 cM,covering 64.4%of the estimated genome ***,a female-specific SCAR marker,CseF-382,was mapped on *** study represents the first genetic linkage map in the tongue *** map has great potential in the identification of quantitative traits loci and sex-related genes and marker-assisted selection in the tongue sole. Meanwhile,the new set of polymorphic microsatellite markers developed in this study is not only useful for genetic mapping but also of critical importance for studies on genetic diversity and broodstock management in tongue sole.

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原发性甲状旁腺功能亢进症骨髓细胞学观察一例

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中华内分泌代谢杂志 2009年第3期25卷 354-355页

作者：朱蕾卢兴国张晓红徐根波赵小英浙江大学医学院附属第二医院血液科杭州310009

原发性甲状旁腺功能亢进症（PHPT）较少见，其骨髓细胞学变化的报告甚少，现报道一例如下：

关键词：原发性甲状旁腺功能亢进症骨髓细胞学变化细胞学观察

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