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关于Chelex 100提取DNA方法的进一步研究——延长保温时间对Chelex 100提取DNA的影响

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河北公安警察职业学院学报 2006年第4期6卷 41-43页

作者：戴文申徐银龙叶健姜成涛赵兴春蒋超中国人民公安大学北京100038 杭州市萧山区公安局浙江杭州311200 公安部物证鉴定中心北京100038

目的探讨用Chelex100法提取血斑DNA时,56℃保温时间、100℃保温时间长短对STR扩增的影响。方法分别改变提取过程中56℃和100℃保温时间,观察其扩增结果。结果56℃这一步骤的保温时间分别设为10min、30min和2h,100℃保温时间设为4min、8... 详细信息

目的探讨用Chelex100法提取血斑DNA时,56℃保温时间、100℃保温时间长短对STR扩增的影响。方法分别改变提取过程中56℃和100℃保温时间,观察其扩增结果。结果56℃这一步骤的保温时间分别设为10min、30min和2h,100℃保温时间设为4min、8min、15min和30min,以上步骤所提取的DNA其扩增结果有明显的差异。结论在一定的时间范围内,每个STR基因座的峰值随着这两步骤保温时间的增加而增加。

关键词：法医物证学 Chelex 100 DNA提取 STR基因座

不同气候变化情景下中国血吸虫病传播的范围与强度预估

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气候变化研究进展 2010年第4期6卷 248-253页

作者：杨坤潘婕杨国静李石柱许吟隆周晓农江苏省血吸虫病防治研究所无锡214064 中国农业科学院农业环境与可持续发展研究所/农业部农业环境与气候变化重点开放实验室北京100081 中国疾病预防控制中心寄生虫病预防控制所上海200025

旨在采用区域气候模型PRECIS模拟的A2、B2两种温室气体排放情景下,预估2050年时段(2046—2050年)和2070年时段(2066—2070年)我国血吸虫病的传播范围和强度的变化状况。结果表明,相对2005年时段(1991—2005年),2050年和2070年时段A2、B... 详细信息

旨在采用区域气候模型PRECIS模拟的A2、B2两种温室气体排放情景下,预估2050年时段(2046—2050年)和2070年时段(2066—2070年)我国血吸虫病的传播范围和强度的变化状况。结果表明,相对2005年时段(1991—2005年),2050年和2070年时段A2、B2情景下血吸虫病分布范围的北界线出现北移,在中国东部尤其是江苏和安徽省境内北移明显。2050年时段,A2、B2情景下的血吸虫病潜在北界线分布相似。长江、洞庭湖及鄱阳湖周围的血吸虫传播指数明显上升,以洞庭湖周围与湖北省内的长江沿线区域上升更加明显。2070年时段,A2情景下血吸虫病潜在北界线的北移趋势明显大于B2情景,进入到山东省境内。血吸虫传播指数进一步增加,A2情景增加的幅度明显大于B2情景。总之,在未考虑将来的适应措施与其他环境因素对血吸虫病传播影响的前提下,A2、B2情景下的血吸虫病的流行区分布和传播指数都将发生明显变化,其中A2情景对我国血吸虫病流行的影响程度大于B2情景。

关键词：气候变化传播范围传播强度血吸虫病预估中国

Anti-lymphangiogenesis for boosting drug accumulation in tumors

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Signal Transduction and Targeted Therapy 2024年第5期9卷 2161-2175页

作者： Chunling Wang Junchao xu Xiaoyu Cheng Ge Sun Fenfen Li Guangjun Nie yinlong Zhang CAS Key Laboratory for Biomedical Effects of Nanomaterials&Nanosafety CAS Center for Excellence in NanoscienceNational Center for Nanoscience and TechnologyBeijing 100190China Sino-Danish College University of Chinese Academy of SciencesBeijing 100190China Sino-Danish Center for Education and Research Beijing 100190China Department of Bioengineering University of PennsylvaniaPhiladelphiaPA 19104USA School of Nanoscience and Engineering University of Chinese Academy of SciencesBeijing 100049China

The inadequate tumor accumulation of anti-cancer agents is a major shortcoming of current therapeutic drugs and remains an even more significant concern in the clinical prospects for *** strategies aiming at regulating the intratumoral permeability of therapeutic drugs have been explored in preclinical studies,with a primary focus on vascular regulation and stromal ***,these methods may trigger or facilitate tumor metastasis as a ***,there is an urgent need for innovative strategies that boost intratumoral drug accumulation without compromising treatment *** another important factor affecting drug tumor accumulation besides vasculature and stroma,the impact of tumor-associated lymphatic vessels(LVs)has not been widely *** the current research,we verified that anlotinib,a tyrosine kinase inhibitor with anti-lymphangiogenesis activity,and SAR131675,a selective VEGFR-3 inhibitor,effectively decreased the density of tumor lymphatic vessels in mouse cancer models,further enhancing drug accumulation in tumor *** combining anlotinib with therapeutic drugs,including doxorubicin(Dox),liposomal doxorubicin(Lip-Dox),and anti-PD-L1 antibody,we observed improved anti-tumor efficacy in comparison with monotherapy ***,this strategy significantly reduced tumor metastasis and elicited stronger anti-tumor immune *** work describes a new,clinically transferrable approach to augmenting intratumoral drug accumulation,which shows great potential to address the current,unsatisfactory efficacies of therapeutic drugs without introducing metastatic risk.

关键词： drugs angiogenesis metastasis

Local resistance characteristics of elbows for supercritical pressure RP-3 flowing in serpentine micro-tubes

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Propulsion and Power Research 2024年第2期13卷 245-256页

作者： Yanchen Fu yinlong Liu Juan Wang Yu Wang Guoqiang xu Jie Wen Research Institute of Aero-engine Beihang UniversityBeijing 100191China National Key Laboratory of Science and Technology on Aero-Engine Aero-thermodynamics Beihang UniversityBeijing 100191China Zhongfa Aviation Institute of Beihang University Hangzhou 310023China AECC Sichuan Gas Turbine Research Institute Chengdu 610500China AECC Hunan Aviation Powerplant Research Institute Zhuzhou 412002China School of Energy and Power Engineering Beihang UniversityBeijing 100191China

Based on the demands of compact heat exchangers and micro cooling channels applied for aviation thermal protection on aero-engines,the elbow localflow resistance charac-teristics for supercritical pressure aviation fuel RP-3flowing in adiabatic horizontal serpentine tubes with the inner diameter of 1.8 mm and the massflux of 1179 kg/(m^(2)·s)were experimen-tally *** long-short-tube method was used to obtain the elbow pressure drop from the total serpentine tube pressure drop,and the effects of system pressures(P/Pc=1.72-2.58)and geometry parameters including bend numbers(n=5-11),bend diameters(D/d=16.7-27.8),and bend distances(L/d=20-60)on elbow pressure drops and local resistance co-efficients are analyzed on the basis of the thermal physical property *** results show that both the increase in the elbow pressure drop and the decrease in the local resistance coef-ficient with temperatures speed up at the near pseudo-critical temperature region of T>*** the growth of the elbow local pressure drop could be inhibited by the increase of system pressures,while the local resistance coefficient is slightly affected by *** influence of bend diameters on the local resistance coefficient is mild when D/d is larger than 22.2 in the premise of fully developedflow in straight ***,a piecewise empir-ical correlation considering the bend diameter and physical property ratio is developed to pre-dict the elbow pressure drop of the serpentine tube and optimize the layout of the cooling tube system on aero-engines.

关键词： Supercritical pressure Aviation fuel Local resistance coefficient Elbow Serpentine tube Empirical correlation

The Potential Distribution Prediction of Oncomelania hupensis Based on Newly Emerging and Reemergent Habitats-China,2015−2019

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China CDC weekly 2021年第5期3卷 90-93,I0001,I0002页

作者： Yanfeng Gong yinlong Li Lijuan Zhang Shan Lv Jing xu Shizhu Li National Institute of Parasitic Diseases Chinese Center for Disease Control and PreventionChinese Center for Tropical Diseases ResearchWHO Collaborating Centre for Tropical DiseasesNational Center for International Research on Tropical DiseasesMinistry of Science and TechnologyKey Laboratory of Parasite and Vector BiologyNational Health Commission of ChinaShanghaiChina

What is already known about this topic?Oncomelania hupensis(***)is the only intermediate host of Schistosoma *** distribution of *** is affected by a series of climate,geographical,sand ocioeconomic factors,which reflect the risk areas of *** is added by this report?There were certain geographical aggregations in the potential high distribution areas of ***,which were mainly distributed in Poyang Lake area,Dongting Lake area,and the middle and the lower reaches of the Yangtze *** are the implications for public health practice?Monitoring and forecasting the distribution of *** is conducive to improving the early warning capabilities of the potential risk of schistosomiasis transmission and progressively promoting the elimination of schistosomiasis nationwide.

关键词： Schistosoma schistosomiasis pens

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丝素固定化凝血酶的工艺研究

中国药师 2016年第4期19卷 671-674页

作者：苗艳丽余广仁徐银龙刘义峰黄慰凤陈绍红广东海洋大学理学院广东湛江524088 广东海洋大学现代生物化学中心

目的:考察家蚕丝素对凝血酶的固定化作用。方法:以家蚕丝素为载体,戊二醛为交联剂制备固定化凝血酶。以固定化凝血酶的活力回收率(AR)为考察指标,采用正交试验确定丝素固定化凝血酶的最佳工艺条件。结果:丝素固定化凝血酶的最佳工艺条件... 详细信息

目的:考察家蚕丝素对凝血酶的固定化作用。方法:以家蚕丝素为载体,戊二醛为交联剂制备固定化凝血酶。以固定化凝血酶的活力回收率(AR)为考察指标,采用正交试验确定丝素固定化凝血酶的最佳工艺条件。结果:丝素固定化凝血酶的最佳工艺条件为:固定时间为6 h,加酶量为2 400 NIH·g^(-1)载体,温度为25℃,p H为7.6。固定化凝血酶的回收率(AR)达67.22%。结论:丝素对凝血酶有良好的固定化作用。

关键词：丝素凝血酶固定化活力回收率

Sentinel Surveillance of Schistosomiasis—China,2021

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China CDC weekly 2023年第12期5卷 278-282页

作者： Suying Guo Hui Dang yinlong Li Lijuan Zhang Fan Yang Junyi He Chunli Cao Jing xu Shizhu Li National Institute of Parasitic Diseases Chinese Center for Disease Control and PreventionChinese Center for Tropical Diseases ResearchNHC Key Laboratory of Parasite and Vector BiologyWHO Collaborating Centre for Tropical DiseasesNational Center for International Research on Tropical DiseasesShanghaiChina

Introduction:This report analyzes the national surveillance data for schistosomiasis in 2021 to understand the current status and provide evidence for further policy actions to promote *** analysis is in line with the National Surveillance Plan of Schistosomiasis,which was revised in 2020 to adapt to the new stage of moving towards ***:Data from the 2021 national surveillance of schistosomiasis in humans,livestock,and snails were collected from 13 provincial-level administrative divisions(PLADs)and analyzed using descriptive epidemiological *** antibody-positive rate and area of newly discovered and re-emergent snail habitats were ***:In 2021,a total of 31,661 local residents and 101,558 transient population were screened for antibodies using indirect hemagglutination assay(IHA).Of those who tested positive,745 local residents and 438 transient population underwent further parasitological examination,with only one stool-positive result in the transient ***,12,966 livestock were examined using the miracidia hatching test,with no positives *** total area of newly discovered and re-emergent snail habitats was 957,702 m^(2) and 4,381,617 m^(2),*** infected snails were found using the microscopic dissection method,but six pooled snail samples were reported as positive using the loopmediated isothermal amplification method for detecting specific sequences of ***,in Anhui and Jiangxi ***:The prevalence of schistosomiasis among humans and livestock was found to be low,however,a potential transmission risk was identified in certain *** reduce the risk of transmission,a comprehensive control strategy should be continued and new techniques should be implemented in the surveillance and early warning system.

关键词： transient elimination Anhui

Correction:Anti-lymphangiogenesis for boosting drug accumulation in tumors

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Signal Transduction and Targeted Therapy 2024年第7期9卷 3141-3142页

作者： Chunling Wang Junchao xu Xiaoyu Cheng Ge Sun Fenfen Li Guangjun Nie yinlong Zhang National Center for Nanoscience and Technology CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety CAS Center for Excellence in Nanoscience Beijing China (GRID:grid.419265.d) (ISNI:0000 0004 1806 6075) University of Chinese Academy of Sciences Sino-Danish College Beijing China (GRID:grid.410726.6) (ISNI:0000 0004 1797 8419) Sino-Danish Center for Education and Research Beijing China (GRID:grid.484648.2) (ISNI:0000 0004 0480 4559) National Center for Nanoscience and Technology CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety CAS Center for Excellence in Nanoscience Beijing China (GRID:grid.419265.d) (ISNI:0000 0004 1806 6075) University of Pennsylvania Department of Bioengineering Philadelphia USA (GRID:grid.25879.31) (ISNI:0000 0004 1936 8972) University of Chinese Academy of Sciences Sino-Danish College Beijing China (GRID:grid.410726.6) (ISNI:0000 0004 1797 8419) University of Chinese Academy of Sciences School of Nanoscience and Engineering Beijing China (GRID:grid.410726.6) (ISNI:0000 0004 1797 8419) National Center for Nanoscience and Technology CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety CAS Center for Excellence in Nanoscience Beijing China (GRID:grid.419265.d) (ISNI:0000 0004 1806 6075)

Correction to:Signal Transduction and Targeted Therapy https://***/10.1038/s41392-024-01794-4,published online 15 April 2024 After the article was published online,1 the authors noticed one inadvertent mistake in Supplementary *** image of lung H&E in the Lip-Dox group was repeatedly inserted as the image of Dox group by mistake during the preparation of the *** correct figure was provided as *** correction did not affect any of our results or discussion presented in the original *** apologize for this inadvertent *** original article has been corrected.

关键词： lung boosting repeatedly

Discovery and evaluation of a novel ^(18)F-labeled vasopressin 1a receptor PET ligand with peripheral binding specificity

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Acta Pharmaceutica Sinica B 2024年第9期14卷 4014-4027页

作者： Junqi Hu yinlong Li Chenchen Dong Huiyi Wei Kai Liao Junjie Wei Chunyu Zhao Ahmad Chaudhary Jiahui Chen Hao xu Ke Zhong Steven H.Liang Lu Wang Weijian Ye Center of Cyclotron and PET Radiopharmaceuticals Department of Nuclear Medicine&Key Laboratory of Basic and Translational Research on Radiopharmaceuticalsthe First Affiliated Hospital of Jinan UniversityGuangzhou 510630China Department of Radiology and Imaging Sciences Emory UniversityAtlant1GA 30322USA Department of Pharmacy Sun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhou 510120China

The arginine-vasopressin(AVP)hormone plays a pivotal role in regulating various physiological processes,such as hormone secretion,cardiovascular modulation,and social *** studies have highlighted the V1a receptor as a promising therapeutic ***-depth insights into V1a receptor-related pathologies,attained through in vivo imaging and quantification in both peripheral organs and the central nervous system(CNS),could significantly advance the development of effective V1a *** address this need,we develop a novel V1a-targeted positron emission tomography(PET)ligand,[^(18)F]V1A-2303([^(18)F]8),which demonstrates favorable in vitro binding affinity and selectivity for the V1a *** tracer binding in peripheral tissues was also confirmed through rigorous cell uptake studies,autoradiography,biodistribution ***,[^(18)F]8 was employed in PET imaging and arterial blood sampling studies in healthy rhesus monkeys to assess its brain permeability and specificity,whole-body distribution,and kinetic *** research indicated[^(18)F]8 as a valuable tool for noninvasively studying V1a receptors in peripheral organs,and as a foundational element for the development of next-generation,brain-penetrant ligands specifically designed for the CNS.

关键词： Vasopressin Autism V1a receptor Positron emission tomography PET imaging Radiolabeling Fluorine 18 Kinetic modeling