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Expression and Significance of Prosaposin (PSAP) in A Rat Model of Letrozole-induced Polycystic Ovary Syndrome

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Journal of Reproduction and Contraception 2013年第2期24卷 67-75页

作者： Yuan-yuan FANG Yi-ning LI Ying-xin ye xiu-xia WANG Department of Gynaecology and Obstetrics Shengjing Hospital of China Medical University

Objective To investigate the prosaposin （PSAP） expression in polycystic ovaries. Methods The expression of PSAP was examined using immunohistochemistry, quantitative RT-PCR and Western blotting in a rat model with letrozole-induced polycystic ovary syndrom （PCOS）. Results Letrozole-induced PCOS rat models were similar to those in human PCOS in endocrine disturbances, metabolic characteristics and morphology features. And in these models both mRNA and protein levels of PSAP were significantly increased. Conclusion PSAP was overexpressed in the letrozole-induced PCOS rat model and might play an important role in the oceurance and development of PCOS.

关键词： polycystic ovary syndrom (PSAP) PCOS rat model (PCOS) androgen receptor (AR) prosaposin

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萃取分光光度法测定氯化钾体系中的十八胺

光谱学与光谱分析 2024年第8期44卷 2240-2249页

作者：宋忠梅路淼张慧芳张鹏瑞房得珍马亮刘青青于雪峰刘海宁叶秀深马珍彭文博吴志坚中国科学院青海盐湖研究所青海省盐湖资源化学重点实验室青海西宁810008 青海盐湖工业股份有限公司盐湖股份中央研究院青海格尔木816000 青海省生产力促进中心有限公司科技事务部青海西宁810008 中国科学院大学化学科学学院北京100049

十八胺是冷结晶-正浮选法生产氯化钾的常用捕收剂,用量显著影响浮选分离效率。由于其会吸附在氯化钾表面,不可避免地在氯化钾中残留,不利于高纯钾盐产品的开发。针对氯化钾生产过程及产品中十八胺含量测定的需求,依据十八胺和溴酚蓝通... 详细信息

十八胺是冷结晶-正浮选法生产氯化钾的常用捕收剂,用量显著影响浮选分离效率。由于其会吸附在氯化钾表面,不可避免地在氯化钾中残留,不利于高纯钾盐产品的开发。针对氯化钾生产过程及产品中十八胺含量测定的需求,依据十八胺和溴酚蓝通过范德华力和氢键络合的作用原理,开发了以乙酸丁酯为萃取剂、溴酚蓝钠盐为显色剂的萃取分光光度法测定氯化钾中十八胺的浓度。考察了溶液pH、十二烷基吗啉、共存盐、平衡时间、显色剂用量对十八胺浓度测量的影响。结果表明:当溶液pH从3~9逐渐增大时,十八胺与溴酚蓝的络合作用减弱且络合分子极性增大,导致吸光度降低;在pH<5时,十二烷基吗啉和溴酚蓝能形成有色络合物,当pH为6~9时萃取液吸光度值趋近于零;共存氯化钾、氯化钠、硫酸钾、氯化镁等溶液的离子强度增大,氢键作用减弱但络合分子的盐析效应增强,溶液中微量的Li^(+)、NH_(4)^(+)和B对萃取液吸光度影响不大;显色剂用量过大时,吸光度值太大且不符合朗伯-比尔定律,平衡时间影响较小。选择测定条件为:溶液的pH为6、离子强度为1 mol·L^(-1),2 mmol·L^(-1)显色剂的用量为0.50 mL,采用缓冲溶液定容至25 mL,反应5 min后加入5.00 mL乙酸丁酯萃取,待分层平衡2 min后于458 nm处测定萃取液吸光度,工作曲线为A=0.04949c+0.06624(R^(2)=0.9923,ε=1.33×10^(4) L·mol^(-1)·cm^(-1),0~10 mg·L^(-1))。该方法的相对标准偏差为0.33%~2.63%,平均相对误差为-0.90%,十八胺和十二烷基吗啉混合体系的平均相对误差为-0.25%。采用该方法测得正浮选精钾母液中十八胺含量为8.66 mg·L^(-1),加标回收率为95.5%~106%,可用于测定盐湖钾肥生产过程中十八胺的浓度。

关键词：十八胺萃取分光光度法氯化钾十二烷基吗啉盐湖

Posterior reversible encephalopathy syndrome in patients with hematologic tumor confers worse outcome

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World Journal of Pediatrics 2015年第3期11卷 245-249页

作者： Hui Li Ying Liu Jing Chen Xia Tan xiu-Yun ye Ming-Sheng Ma Jian-Ping Huang Li-Ping Zou Department of Pediatrics Chinese PLA General HospitalBeijing 100583China Department of Pediatrics Xin Hua Hospital and Shanghai Children's Medical CenterShanghai Second Medical UniversityShanghai 200127China Department of Radiology The Yuying Children Hospital Affiliated Wenzhou Medical UniversityWenzhou 325027China Department of Pediatrics Peking Union Medical College HospitalBeijing 1007320China Department of Nephrology and Rheumatology Bayi Children's Hospital Affiliated to Beijing Military Region General HospitalBeijing 100700China

Background:This study aimed to evaluate the clinical features of posterior reversible encephalopathy syndrome(PRES)in ***:The medical records of 31 patients from five medical centers who were diagnosed with PRES from 2001 to 2013 were retrospectively *** the 31 patients,16 were males,and 15 females,with a median age of 7 years(3-12 years).Patients younger than 10 years accounted for 74.2%of the 31 ***:Seizure,the most common clinical sign,occurred in 29 of the 31 *** disturbances were also observed in 20 *** imaging abnormalities were bilateral and predominant in the parietal and occipital white *** this series,three patients died in the acute phase of *** patient had resolution of neurologic presentation within one week,but no apparent improvement in radiological abnormalities was observed at eight *** patient showed gradual recovery of both neurologic presentation and radiological abnormalities during follow-up at eight *** patient developed long-term cortical *** of the PRES patients with hematologic tumor had a worse prognosis than those without hematologic ***:Seizure is a prevalent characteristic of children with *** prognosis can be seen in PRES patients with hematologic tumor.

关键词： adverse outcomes posterior reversible encephalopathy syndrome seizure tumors

A Trichophyton Rubrum Infection Model Based on the Reconstructed Human Epidermis - Episkin

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Chinese Medical Journal 2016年第1期129卷 54-58页

作者： Pan-Pan Liang Xin-Zhu Huang Jin-Ling Yi Zhi-Rui Chen Han Ma Cong-xiu ye Xian-Yan Chen Wei Lai Jian Chen Department of Dermatology Third Affiliated Hospital of Sun Yat-sen University Guangzhou Guangdong 510630 China

Background： Trichophyton rubrum represents the most common infectious fungus responsible for dermatophytosis in human, but the mechanism involved is still not completely understood. An appropriate model constructed to simulate host infection is the prerequisite to study the pathogenesis of dernlatophytosis caused by T.. rubrum. In this study, we intended to develop a new T. rubrum infection model in vitro, using the three-dimensional reconstructed epidermis - EpiSkin, and to pave the way for further investigation of the mechanisms involved in T. rubrum infection. Methods： The reconstructed human epidermis （RHE） was infected by inoculating low-dose （400 conidia） and high-dose （4000 conidia） T. rubrum conidia to optimize the infection dose. During the various periods after infection, the samples were processed for pathological examination and scanning electron microscopy （SEM） observation. Results： The histological analysis of RHE revealed a fully differentiated epidermis with a functional stratum corneum, which was analogous to the normal human epidermis. The results of hematoxylin and eosin staining and the periodic acid-Schiff staining showed that the infection dose of 400 conidia was in accord with the pathological characteristics of host dermatophytosis caused by T. rubrum. SEM observations further exhibited the process of 77 ruhrum infection in an intuitionistic way, Conclusions： We established the T. rubrum infection model on RHE in vitro successfully. It is a promising model fbr further investigation of the mechanisms involved in T. rubrum infection.

关键词： EpiSkin Infection Model: Trichophyton Rubrum

Ginsenoside Rg3 Promotes the Survival and Migration of Trophoblast Cells in a Rat Model of Gestational Hypertension by Regulating miR-100a/Insulin Growth Factor-2 Axis

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World Journal of Traditional Chinese Medicine 2024年第1期10卷 65-74页

作者： xiu-Mei Fan Li Yang Gang Zhao Sen-ye Huo Yan Gao Department of Obstetrics Xingtai Third HospitalXingtaiChina Department of Obstetrics Xingtai Maternal and Child Health Care HospitalXingtaiChina

Objective:Preeclampsia(PE)is a common complication during ***-100a is expressed in the placenta and regulates the survival and development of placental *** growth factor-2(IGF-2)may serve as its downstream *** study investigated the protective mechanisms of ginsenoside Rg3 against PE in rat *** and Methods:LPS-induced rat PE models were suitable for intravenous administration of the highly expressed miR-100a ginsenoside Rg3 lentiviral *** trophoblasts were cultured in vitro for JEG-3,and PE cell models were *** vivo effects on tumor growth and apoptosis were *** Rg3 was treated with different concentrations of shRNA,miR-100a analogs,inhibitors,or *** and the expression of autophagy-related proteins were *** activity and migration were determined using Cell Counting Kit-8 and Transwell *** drugs strongly inhibited trophoblasts under normal conditions with some synergy between ***-luciferase return assay confirmed the binding affinity of miR-100a for ***:In response to Rg3,autophagy and the expression of autophagy-related proteins LC3-I/II,Beclin1,and SQSTM1 were reduced in PE rat placental ***3 inhibited autophagy in JEG-3 cells and promoted JEG-3 survival and migration in a concentration-dependent ***-100a upregulated PE *** results suggested that autophagy was a vital signaling ***3 intervention inhibited miR-100a expression and miR-100a downregulated IGF-2 expression in placental tissues and promoted autophagy,thereby inhibiting JEG-3cell survival and *** rats,Rg3 inhibited PE development by regulating the activity of the miR-100a-IGF-2 signaling ***:Ginsenoside Rg3 positively regulates the miR-100a-IGF-2 axis and protects PE rats by inhibiting trophoblastic autophagy and promoting trophoblastic cell survival and migration.

关键词： Ginsenoside Rg3 inflammatory response miR-100a/insulin growth factor-2 axis preeclampsia pregnancy-induced hypertension

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互补或替代:保税区与出口加工区的贸易效应

国际经贸探索 2017年第5期33卷 40-50,94页

作者：叶修群华东师范大学经济与管理学部

保税区和出口加工区作为实施改革开放战略的核心环节,对外向型经济发展的重要性不言而喻。文章利用2000~2013年省级面板数据分析了保税区和出口加工区的贸易效应及其相互作用,研究发现保税区和出口加工区的贸易效应体现在出口上,且两者... 详细信息

保税区和出口加工区作为实施改革开放战略的核心环节,对外向型经济发展的重要性不言而喻。文章利用2000~2013年省级面板数据分析了保税区和出口加工区的贸易效应及其相互作用,研究发现保税区和出口加工区的贸易效应体现在出口上,且两者存在明显的替代;保税区和出口加工区促进了中西部出口贸易发展,且两者呈替代关系。进一步分析发现出口加工区的加工贸易和一般贸易效应显著为正,且与保税区呈替代关系;保税区和出口加工区的加工贸易和一般贸易效应在中西部均显著为正,且相互替代;出口加工区促进了东部加工贸易出口的发展,并与保税区存在互补作用。

关键词：保税区出口加工区贸易效应加工贸易一般贸易

我国自由贸易园区(FTZ)的FDI引致效应实证研究

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国际经贸探索 2016年第2期32卷 81-90页

作者：叶修群华东师范大学商学院上海200241

采用1993-2013年28省市面板数据,利用投资引力模型分析我国自由贸易园区的FDI引致效应。研究表明：我国自由贸易园区的FDI引致效应明显,但总体呈弱化趋势;自由贸易园区的FDI引致效应具有明显的区域性差异,东部地区和西部地区自由贸易园... 详细信息

采用1993-2013年28省市面板数据,利用投资引力模型分析我国自由贸易园区的FDI引致效应。研究表明：我国自由贸易园区的FDI引致效应明显,但总体呈弱化趋势;自由贸易园区的FDI引致效应具有明显的区域性差异,东部地区和西部地区自由贸易园区的FDI引致效应显著为正,中部地区自由贸易园区的FDI引致效应为负;各区域自由贸易园区的FDI引致效应的变化趋势具有明显的区域性差异,东部地区自由贸易园区的FDI引致效应弱化、中部地区为强化、西部地区则是先强化后弱化。

关键词：自由贸易园区外商直接投资引致效应引力模型

保税区与出口加工区对工业企业选址的影响——基于中国省级面板数据的实证分析

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中央财经大学学报 2018年第10期 96-104页

作者：叶修群江西师范大学商学院

作为重要的地方指向型对外开放政策,保税区和出口加工区能够通过税收优惠政策和贸易便利化措施降低工业企业的贸易成本,进而吸引工业企业在园区及其周边地区投资设厂。笔者以规模以上工业企业为研究对象,基于2000—2014年省级面板数据,... 详细信息

作为重要的地方指向型对外开放政策,保税区和出口加工区能够通过税收优惠政策和贸易便利化措施降低工业企业的贸易成本,进而吸引工业企业在园区及其周边地区投资设厂。笔者以规模以上工业企业为研究对象,基于2000—2014年省级面板数据,运用面板负二项回归实证检验了保税区和出口加工区对工业企业选址的影响及其异质性。研究发现:保税区和出口加工区显著提高了所在地区对工业企业的吸引力,且两者之间不存在明显的替代关系,但区位劣势限制了保税区对工业企业的吸引作用;保税区和出口加工区有利于私营工业企业选址,但两者之间存在明显的替代关系;同时,保税区和出口加工区也促进了国有和外资工业企业由东向西转移。据此,笔者得出相关研究结论并有针对性地提出政策性建议:政府应该利用自由贸易试验区整合保税区和出口加工区,并在园区内试验制度创新,创造竞争中立的投资环境,实现引进企业所有制类型多元化,不同地区自由贸易试验区招商引资应各有侧重,等等。

关键词：保税区出口加工区工业企业企业选址

“一带一路”战略下我国自由贸易园区的贸易效应研究

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广东财经大学学报 2016年第2期31卷 25-32页

作者：叶修群华东师范大学商学院上海200241

通过构建理论模型阐释自由贸易园区对对外贸易的影响,进而利用1993年-2013年28省的面板数据实证分析我国自由贸易园区的贸易效应。结果表明：我国自由贸易园区的贸易效应显著,且呈明显的强化趋势;东部自由贸易园区的贸易效应总体呈强化... 详细信息

通过构建理论模型阐释自由贸易园区对对外贸易的影响,进而利用1993年-2013年28省的面板数据实证分析我国自由贸易园区的贸易效应。结果表明：我国自由贸易园区的贸易效应显著,且呈明显的强化趋势;东部自由贸易园区的贸易效应总体呈强化趋势,但其进口贸易效应则明显弱化;中部自由贸易园区的贸易效应总体呈强化趋势,但进口贸易效应明显弱化;西部自由贸易园区的贸易效应显著,但总体呈明显弱化趋势。因而东中西部自由贸易园区的建设和发展应因地制宜,在＂一带一路＂发展战略中发挥出各自的特色和优势。

关键词：对外贸易贸易自由化自由贸易园区贸易效应一带一路进出口贸易