检索结果-南通市图书馆

Tumor suppressor function of ezrin-radixin-moesin-binding phosphoprotein-50 through β-catenin/E-cadher in pathway in human hepatocellular cancer

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2013年第8期19卷 1306-1313页

作者： xiu-Lan Peng Meng-Yao Ji zi-Rong yang Jia Song Wei-Guo Dong Department of Gastroenterology Renmin Hospital of Wuhan University

AIM:To determine the effect and molecular mechanism of ezrin-radixin-moesin-binding phosphoprotein-50(EBP50) in hepatocellular carcinoma(HCC).METHODS:Three human HCC cell lines,i.e.,SMMC7721,HepG2 and Hep3B,were *** transfected the Pbk-CMV-HA-EBP50 plasmid into SMMC7721 cells with Lipofectamine 2000 to overexpress *** blotting were performed to determine the effects of the plasmid on EBP50 expression and to detect the expression of β-catenin and E-cadherin before and after the transfection of the plasmid into SMMC7721 *** vitro cell proliferation was assessed with a Cell Counting Kit-8(CCK-8) *** cycle distribution was assessed with flow *** and migration ability of before and after the transfection were determined with a transwell *** apoptosis was demonstrated with Annexin *** effect of EBP50 overexpressing on tumor growth in vivo was performed with a xenograft tumor model in nude ***:The transfection efficiency was confirmed with Western blotting(1.36 ± 0.07 vs 0.81 ± 0.09,P < 0.01).The CCK8 assay demonstrated that the growth of cells overexpressing EBP50 was significantly lower than control cells(P < 0.01).Cell cycle distribution showed there was a G0/G1 cell cycle arrest in cells overexpressing EBP50(61.3% ± 3.1% vs 54.0% ± 2.4%,P < 0.05).The transwell assay showed that cell invasion and migration were significantly inhibited in cells overexpressing EBP50 compared with control cells(5.8 ± 0.8 vs 21.6 ± 1.3,P < 0.01).Annexin V-FITC revealed that apoptosis was significantly increased in cells overexpressing EBP50 compared with control cells(14.8% ± 2.7% vs 3.4% ± 1.3%,P < 0.05).The expression of β-catenin was downregulated and E-cadherin was upregulated in cells overexpressing EBP50 compared with control cells(0.28 ± 0.07 vs 0.56 ± 0.12,P < 0.05;0.55 ± 0.08 vs 0.39 ± 0.07,P < 0.05).In vivo tumor growth assay confirmed that up-regulation of EBP50 could obviously slow the growth of HCC derived from SMM

关键词： Hepatocellular carcinoma Ezrin-radixinmoesin-binding phosphoprotein-50 Growth Migration Invasion

Overexpression of carbonic anhydrase 1 in pterygium

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2016年第6期9卷 931-932页

作者： Qian yang zi-Yan Chen xiu-Ping Liu Kai-Li Wu Zhongshan Ophthalmic Center State Key Laboratory of OphthalmologySun Yat-sen University

Carbonic anhydrase （CA） is a kind of zinc enzyme that reversibly catalyzes hydration of CO2. Sixteen CAisoenzymes have been described in mammals . These isozymes play physiological roles in erythrocytes, including CO2 transport, ion secretion, pH regulation and so forth. CA1 and 2 are both cytosolic enzymes that are found in large quantities in erythrocytes. Excluding hemoglobin, CA I is the most abundant protein in erythrocytes

关键词： Overexpression of carbonic anhydrase 1 in pterygium

Mesh parameterization based on edge collapse

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2009年第8期10卷 1153-1159页

作者： Jiang QIAN xiu-zi YE Cui-hao FANG San-yuan ZHANG State Key Lab of CAD & CG School of Computer Science and Technology Zhejiang University Hangzhou 310027 China

Parameterizations that use mesh simplification to build the base domain always adopt the vertex removal *** paper applies edge collapse to constructing the base domain *** inducing the parameterization of the original mesh over the base domain,new algorithms map the new vertices in the simplified mesh back to the original one according to the edge transition sequence to integrate the *** present a direct way,namely edge classification,to deduce the *** results show that the new parameterization features considerable saving in computing complexity and maintains smoothness.

关键词： Edge collapse Vertex removal Mapping Edge classification

Effects of Thermally Assisted Warm Laser Shock Processing on the Microstructure and Fatigue Property of IN718 Superalloy

在线全文

学校读者我要写书评

暂无评论

Acta Metallurgica Sinica(English Letters) 2021年第12期34卷 1645-1656页

作者： yang Liu Lei Wang Kaiyue yang xiu Song The Key Laboratory for Anisotropy and Texture of Materials(Ministry of Education) School of Materials Science and EngineeringNortheastern UniversityShenyang 110819China

The effects of laser shock processing(LSP)and warm laser shock processing(WLSP)on the microstructure of surface hardening layer and high-cycle fatigue performance at room temperature and high temperature(600°C)of IN718 alloy were *** has been revealed that the grain refined hardening layer with greater residual compression stresses,higher fraction of coincidence site lattice(CSL)boundaries and dislocation densities was formed in WLSP-treated alloy than in LSP-treated ***,microtwins includedγ″phase/high density dislocation complex was found in the surface of WLSP-treated *** characters caused the significant enhancement of the medium value fatigue strength of WLSP-treated alloy at room temperature and elevated ***,the microtwins includedγ″phase/high density dislocation complex formed in the surface hardening layer of LSP-treated alloy has more complicated steric structure and more stable at elevated temperature thanγ″phase/low density dislocation complex formed in LSP-treated alloy,leading to the slow recovery ***,the surface hardening layer in the WLSP-treated alloy remained more ideal strengthening effect under high-cycle fatigue at elevated temperature than that in LSP-treated *** resulted in the much longer fatigue crack initiation incubation and longer high-cycle life of WLSP-treated IN718 alloy under cycling load at 600℃.This discovery provides a new cognition of fatigue resistance by WLSP treatment of precipitation strengthening superalloy.

关键词： IN718 superalloy Warm laser shock processing(WLSP) Microstructure Fatigue Residual stress

Integrated phase unwrapping algorithm for the measurement of 3D shapes by Fourier transform profilometry

在线全文

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2009年第7期10卷 1018-1028页

作者： Shuang-qing WU Yin ZHANG San-yuan ZHANG xiu-zi YE School of Computer Science and Technology Zhejiang University Hangzhou 310027 China

An integrated and reliable phase unwrapping algorithm is proposed based on residues and blocking-lines detection, closed contour extraction and quality map ordering for the measurement of 3D shapes by Fourier-transform profilometry (FTP). The proposed algorithm first detects the residues on the wrapped phase image, applies wavelet analysis to generate the blocking-lines that can just connect the residues of opposite polarity, then carries out the morphology operation to extract the closed contour of the shape, and finally uses the modulation intensity information and the Laplacian of Gaussian operation of the wrapped phase image as the quality map. The unwrapping process is completed from a region of high reliability to that of low reliability and the blocking-lines can prevent the phase error propagation effectively. Furthermore, by using the extracted closed contour to exclude the invalid areas from the phase unwrapping process, the algorithm becomes more efficient. The experiment shows the effec-tiveness of the new algorithm.

关键词： Fourier-transform profilometry (FTP) Phase unwrapping Residues Blocking-lines Laplacian of Gaussian Modulation intensity

A review of the strengthening–toughening behavior and mechanisms of advanced structural materials by multifield coupling treatment

在线全文

学校读者我要写书评

暂无评论

International Journal of Minerals,Metallurgy and Materials 2022年第2期29卷 185-199页

作者： xiu Song Lei Wang yang Liu The Key Laboratory for Anisotropy and Texture of Materials(Ministry of Education) School of Materials Science and EngineeringNortheastern UniversityShenyang 110819China

The application of an external field is a promising method to control the microstructure of materials, leading to their improved performance. In the present paper, the strengthening and toughening behavior of some typical high-performance structural materials subjected to multifield coupling treatment, including electrostatic field, electro-pulse current, thermal field, and stress field, are reviewed in detail. In addition to the general observation that the plasticity of materials could be increased by multi-external fields, strength enhancement can be achieved by controlling atomic diffusion or phase transformations. The paper is not limited to the strengthening and toughening mechanisms of the multifield coupling effects on different types of structural materials but is intended to provide a generic method to improve both the strength and ductility of the materials. Finally, the prospects of the applications of multi-external fields have also been proposed based on current works.

关键词： multifield coupling structural materials strengthening toughening microstructure

Inhibitory effects of luteolin on TLR3-mediated inflammation caused by TAK/NF-κB signaling in human corneal flbroblasts

在线全文

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2022年第3期15卷 371-379页

作者： zi-Han Guo Ping-Ping Liu Heng Wang xiu-Xia yang Cheng-Cheng yang Hui Zheng Dan Tang yang Liu Department of Ophthalmology the Fifth Affiliated HospitalSun Yat-sen UniversityZhuhai 519000Guangdong ProvinceChina

AIM: To study the role of luteolin(LUT) in the expression of toll-like receptors 3(TLR3) ligand poly I:C stimulated inflammatory factors in human corneal fibroblasts(HCFs).METHODS: HCFs cells were cultivated with or without LUT or poly I:*** expression levels of interleukin(IL)-6, IL-8, monocyte chemotactic protein-1(MCP-1), vascular cell adhesion molecule(VCAM)-1, as well as intercellular adhesion molecule(ICAM)-1 were measured using enzymelinked immunosorbent assay(ELISA), immunoblotting or reverse transcription-quantitative polymerase chain reaction(PCR) *** was used to assess toll-interleukin-1 receptor-domain-containing adapter-inducing interferon-β(TRIF), TLR3, transforming growth factor-bactivated kinase 1(TAK1), tumor necrosis factor receptor-associated factor 6(TRAF6), the transcription factor AP-1, as well as transcription factor nuclear factor(NF-κB)–inhibitory protein IκB-α degradation and *** assays were used to localize the cellular location of the p65 subunit of NF-κ***: Corneal fibroblasts exposed to poly I:C demonstrated decreased VCAM-1, ICAM-1, MCP-1, IL-6, and IL-8 expression levels upon exposure to LUT in a time-dependent and concentration-dependent *** was observed to suppress poly I:C-triggered expression of TLR3, the translocation of NF-κB p65 into cell nuclei, as well as the phosphorylation of TAK, c-Jun, and IκB-α, while no impact on the expression levels of TRIF and TRAF6 were ***: LUT suppress the expression of proinflammatory adhesion molecules, chemokines, and cytokines in poly I:C exposed *** effects are likely mediated through TAK/NF-κB signal ***, LUT is a candidate molecule that can prevent the TLR3-mediated inflammation response associated with corneal viral infection.

关键词： toll-like receptor inflammation corneal fibroblast poly I:C

Solvent-tuned polyoxometalate-based supramolecular hybrids constructed from different metal-organic motifs:Various structures and adsorption properties for dyes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2018年第2期29卷 309-312页

作者： Shan Zhang Xiang Wang Hui-xiu Zhang zi-Hui Zhao xiu-Li Wang Department of Chemistry Bohai University

By changing the solvents, three polyoxometalate（POM）-based supramolecular hybrids, [Co2 L3][SiW（12）O（40）]·17 H2 O（1）,[Co2 L2（H2 O）4][SiW（12）O（40）].2 EtOH·2 H2 O（2） and [Co2 L3][SiW（12）O（40）]·9 DMA·6 H2 O（3）, have been synthesized under hydrothermal or solvothermal conditions. All three hybrids show various supramolecular structures, which contain two kinds of different metal-organic motifs. A kind of metal-organic cationic cage [Co2 L3]^4＋ is formed in the presence of H2 O for 1, DMA/H2 O for 3, and a metalorganic cationic circle [Co2 L2]^（4＋） is constructed in the presence of EtOH/H2 O for 2. That is to say, the solvents show a key role in tuning the architectures of metal-organic fragments and the final POM-based supramolecular structures. The adsorption properties of 1-3 for different organic dyes have also been investigated.

关键词： Polyoxometalate Solvent-tuned Bis-imidazolyl-bis-amide ligand Dyes adsorption Supramolecular hybrids

Optimal dispatch of multi energy system using power-to-gas technology considering flexible load on user side

在线全文

学校读者我要写书评

暂无评论

Frontiers in Energy 2018年第4期12卷 569-581页

作者： zi LING xiu yang zilin LI College of Electric Engineering Shanghai University of Electric PowerShanghai 200090China

The relation between power-to-gas technology (P2G) and energy interconnection becomes increasingly close. Meanwhile, the participation of flexible load on user side in system optimization has attracted much attention as an efficient approach to relieve the contradiction between energy supply and energy demand. Based on the concept of energy hub, according to its series characteristic, this paper established a generic multi-energy system model using the P2G technology. The characteristic of flexible load on user side was considered and optimal dispatch analysis was made, so as to reduce the cost, to reasonably dispatch the flexible load, to reduce the discharge, to enhance the new energy output, and to increase the power-to-gas conversion efficiency. Finally, a concrete analysis was made on the optimal dispatch result of the multi-energy system using the P2G technology considering flexible load on user side in the calculating example, and optimal dispatch of the system was verified via four different scenarios. The results indicate that cooperative dispatch of multi-energy system using the P2G technology considering flexible load on user side is the most economic, and can make a contribution to absorption of new energy and P2G conversion. In this way, environmental effects and safe and stable operation of the system can be guaranteed.

关键词： multi-energy system energy hub series characteristic optimal dispatch flexible load