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Comparison of the virulence plasmid genomes of two strains of Shigella which lost the ability to bind Congo red

Science china(Life Sciences) 2006年第2期49卷 141-148页

作者： xiong zhaohui1. tang xudong1.2, yang fan1. zhang xiaobing1. yang jian1. chen lihong1. nie huan1. yan yongliang1. jiang yan1. wang jing1. xue Ying1, xu xingye1. zhu yafang1. dong jie1. AN lizhe2, wang xunling2 & jin qi1.1. state key laboratory for molecular virology and genetic engineering, beijing 1.0052, china 2. School of Life Science, Lanzhou University, Lanzhou, 743000) 1. state key laboratory for molecular virology and genetic engineering Beijing 100052 China 2. School of Life Science Lanzhou University Lanzhou 743000 China

We determined; analyzed the Shigella flexneri serotype 5 (pSF5); S. dysenteriae serotype 1 (pSD1) virulence plasmid genomes. The total length of pSF5 is 136513 bp, including 165 open reading frames (ORFs). Of these ORFs, 133 were identified; 32 of those had no significant homology to proteins with known functions. The length of pSD1 is 1.2545 bp, including 224 ORFs, of which we identified 1.1. The remaining 43 ORFs were not significantly homologous to proteins with known functions. The insertion sequence (IS) elements are 53787 bp in pSF5,; 49616 bp in pSD1, which represents 39.4%; 27.1% of the genome, respectively. There are 22 IS element types in pSF5; pSD1, among which we report ISEc8; ISSbo6 for the first time in the Shigella virulence plasmid. Compared to pCP301, there are a large number of deleted genes; gene inversions in both pSF5; pSD1. The ipa-mxi-spa locus in pSF5 is completely absent,; the genes related to the O-antigen biosynthesis are partially missing. In contrast, the above genes in pSD1 are integral, with the exception of virF. The whole genome analysis of the two plasmids shows that the loss of genes related to gene invasion or regulation also obliterates the ability of pPF5; pSD1 to bind Congo red (Crb). Whether these genes determine the Crb function requires continued investigation.

关键词： Shigella flexneir serotype 5, Shigella dysenteriae serotype 1, virulence plasmid, Congo red, genome comparison.

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