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Additive manufacturing of promising heterostructure for biomedical applications

International Journal of Extreme Manufacturing 2023年第3期5卷 363-405页

作者： Cijun Shuai Desheng Li xiong yao Xia Li Chengde Gao State Key Laboratory of Precision Manufacturing for Extreme Service Performance College of Mechanical and Electrical EngineeringCentral South UniversityChangsha 410083People’s Republic of China Institute of Additive Manufacturing Jiangxi University of Science and TechnologyNanchang 330013People’s Republic of China Research Center for Macromolecules and Biomaterials National Institute for Materials Science(NIMS)1-1NamikiTsukubaIbaraki 305-0044Japan

As a new generation of materials/structures,heterostructure is characterized by heterogeneous zones with dramatically different mechanical,physical or chemical *** endows heterostructure with unique interfaces,robust architectures,and synergistic effects,making it a promising option as advanced biomaterials for the highly variable anatomy and complex functionalities of individual ***,the main challenges of developing heterostructure lie in the control of crystal/phase evolution and the distribution/fraction of components and *** recent years,additive manufacturing techniques have attracted increasing attention in developing heterostructure due to the unique flexibility in tailored structures and synthetic *** review focuses on the additive manufacturing of heterostructure for biomedical *** structural features and functional mechanisms of heterostructure are *** typical material systems of heterostructure,mainly including metals,polymers,ceramics,and their composites,are *** the resulting synergistic effects on multiple properties are also systematically discussed in terms of mechanical,biocompatible,biodegradable,antibacterial,biosensitive and magnetostrictive ***,this work outlines the research progress of additive manufacturing employed in developing heterostructure from the aspects of advantages,processes,properties,and *** review also highlights the prospective utilization of heterostructure in biomedical fields,with particular attention to bioscaffolds,vasculatures,biosensors and ***,future research directions and breakthroughs of heterostructure are prospected with focus on their more prospective applications in infection prevention and drug delivery.

关键词： additive manufacturing heterostructure synergistic effects integrated properties biomedical applications

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渐变截面梁波透射反射系数求解的数值验证

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振动与冲击 2011年第3期30卷 274-280页

作者：林耀雄厦门市公路局厦门361009 福州大学土木工程学院福州350002

推导任意截面形状的预应力Timoshenko梁波动微分方程,对预应力箱板梁渐变截面段弹性波透(反)射系数矩阵进行计算,为求得小端传播到大端的透射波幅值,将渐变截面梁近似当作具有一系列截面间断的阶梯梁,按层状结构波传播的传递矩阵法求波... 详细信息

推导任意截面形状的预应力Timoshenko梁波动微分方程,对预应力箱板梁渐变截面段弹性波透(反)射系数矩阵进行计算,为求得小端传播到大端的透射波幅值,将渐变截面梁近似当作具有一系列截面间断的阶梯梁,按层状结构波传播的传递矩阵法求波幅在大小端间的透(反)射系数矩阵,通过基于Maple软件平台编写子程序对梁截面尺寸及渐变段分段数目对透(反)射系数矩阵精确性的影响进行数值分析。在波动微分方程伽辽金近似解基础上,对渐变截面段梁波透(反)射系数矩阵的求解法进行数值验证,验证中以第一类贝塞尔函数组合作为系数具有高次项幂级数函数的高阶常微分方程伽辽金法近似解的试函数,该试函数对这类方程具有普遍的适用性。

关键词：渐变截面梁透(反)射系数矩阵伽辽金法弹性波贝塞尔函数组合

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Protease inhibitor ASP enhances freezing tolerance by inhibiting protein degradation in kumquat

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Horticulture Research 2023年第4期10卷 53-62页

作者： Hua Yang Ke-wei Qiao Jin-jing Teng Jia-bei Chen Ying-li Zhong Li-qun Rao Xing-yao xiong Huang Li College of Bioscience and Biotechnology Hunan Agricultural UniversityChangsha 410128China Hunan Provincial Key Laboratory for Germplasm Innovation and Crop Utilization Hunan Agricultural UniversityChangsha 410128China Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural SciencesShenzhen 518000China Center for Plant Science Innovation University of Nebraska-LincolnLincolnNE 68588USA

Cold acclimation is a complex biological process leading to the development of freezing tolerance in *** this study,we demonstrated that cold-induced expression of protease inhibitor FmASP in a Citrus-relative species kumquat[Fortunella margarita(Lour.)Swingle]contributes to its freezing tolerance by minimizing protein ***,we found that only cold-acclimated kumquat plants,despite extensive leaf cellular damage during freezing,were able to resume their normal growth upon stress *** dissect the impact of cold acclimation on this anti-freezing performance,we conducted protein abundance assays and quantitative proteomic analysis of kumquat leaves subjected to cold acclimation(4◦C),freezing treatment(−10◦C)and post-freezing recovery(25◦C).FmASP(Against Serine Protease)and several non-specific proteases were identified as differentially expressed proteins induced by cold acclimation and associated with stable protein abundance throughout the course of low-temperature *** was further characterized as a robust inhibitor of multiple *** addition,heterogeneous expression of FmASP in Arabidopsis confirmed its positive role in freezing ***,we proposed a working model of FmASP and illustrated how this extracellular-localized protease inhibitor protects proteins from degradation,therebymaintaining essential cellular function for post-freezing *** findings revealed the important role of protease inhibition in freezing response and provide insights on how this role may help develop new strategies to enhance plant freezing tolerance.

关键词： freezing inhibiting thereby

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Exact free vibration analysis of open circular cylindrical shells by the method of reverberation-ray matrix

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2016年第4期17卷 295-316页

作者： xiong-liang yao Dong TANG Fu-zhen PANG Shuo LI College of Shipbuilding Engineering Harbin Engineering University Harbin 150001 China

This paper is concerned with the free vibration analysis of open circular cylindrical shells with either the two straight edges or the two curved edges simply supported and the remaining two edges supported by arbitrary classical boundary conditions. Based on the Donnell-Mushtari-Vlasov thin shell theory, an analytical solution of the traveling wave form along the simply supported edges and the modal wave form along the remaining two edges is obtained. With such a unidirectional traveling wave form solution, the method of the reverberation-ray matrix is introduced to derive the equation of natural frequencies of the shell with different classical boundary conditions. The exact solutions for natural frequencies of the open circular cylindrical shell are obtained with the employment of a golden section search algorithm. The calculation results are compared with those obtained by the finite element method and the methods in the available literature. The influence of length, thickness, radius, included angle, and the boundary conditions of the open circular cylindrical shell on the natural frequencies is investigated. The exact calculation results can be used as benchmark values for researchers to check their numerical methods and for engineers to design structures with thin shell components.

关键词： Open circular cylindrical shell Method of reverberation-ray matrix Free vibration analysis Donnell-Mushtari- Vlasov thin shell theory Analytical wave form solution

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Development of a multiplex reverse transcription-PCR assay for simultaneous detection of garlic viruses

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Journal of Integrative Agriculture 2015年第5期14卷 900-908页

作者： HU Xin-xi LEI Yan WANG Pei TANG Lin-fei HE Chang-zheng SONG Yong xiong Xing-yao NIE Xian-zhou Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization Hunan Provincial Engineering Research Center for PotatoesCollege of Horticulture and LandscapeHunan Agricultural University Potato Research Centre Agriculture and Agri-Food Canada

A preliminary screening for garlic viruses in garlic plants in Hunan,China,using existing monoplex（simplex） reverse transcription-polymerase chain reaction（RT-PCR） procedures detected four viruses/virus *** viruses/virus groups were Onion yellow dwarf virus（OYDV）,Leek yellow stripe virus（LYSV）,Shallot latent virus（SLV） and allexiviruses（e.g.,garlic viruses A,B,C,D,E,X）.Sequence analysis of the projected allexivirus amplicons revealed the allexivirus in the infected garlic plants was Garlic virus D（GarV-D）,which shared 92-97%sequence identities with various isolates from the world.A multiplex RT-PCR（mRT-PCR） was therefore developed to simultaneously detect and differentiate the four viruses/virus *** achieve this,four primer pairs targeting allexiviruses,OYDV,LYSV and SLV were *** anticipated amplicon sizes are 183 bp（allexiviruses）,265 bp（OYDV）,404 bp（LYSV） and 592 bp（SLV）,*** primer pairs produced virus-specific fragments in both simplex and multiplex formats,thus confirming the efficacy of the newly developed mRT-PCR for detection of these *** mRT-PCR further was evaluated by applying it to garlic plant samples collected in two geographic locations in ***,OYDV,LYSV and SLV were detected in 50.9,40.3,28.3 and 58.5%of leaf samples,respectively;and mixed infections with two or more viruses accounted for 54%of the garlic *** results obtained by mRT-PCR were confirmed by simplex RT-PCR *** conclusion,this newly developed mRT-PCR provides a rapid,sensitive and reliable method for the detection and identification of major garlic viruses.

关键词： simplex multiplex yellow latent primer simultaneous accounted Hunan targeting geographic

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PAPP2C Interacts with the Atypical Disease Resistance Protein RPW8.2 and Negatively Regulates Salicylic Acid-Dependent Defense Responses in Arabidopsis

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Molecular Plant 2012年第5期5卷 1125-1137页

作者： Wen-Ming Wang Xian-Feng Ma Yi Zhang Ming-Cheng Luo Guo-Liang Wang Maria Bellizzi Xing-yao xiong Shun-Yuan Xiao Institute for Bioscience and Biotechnology Research and Department of Plant Sciences and Landscape Architecture University of Maryland Rockville MD20850 USA Rice Research Institute Sichuan Agricultural University Chengdu 611130 China Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop Hunan Agricultural University Changsha 410128 China Department of Plant Sciences University of.Califor.nia. Davis: CA 95616 USA Department of Plant Pathology The Ohio State University Columbus OH 43210 USA

Many fungal and oomycete pathogens differentiate a feeding structure named the haustorium to extract nutrition from the plant epidermal cell. The atypical resistance （R） protein RPW8.2 activates salicylic acid （SA）-dependent, haustorium-targeted defenses against Golovinomyces spp., the causal agents of powdery mildew diseases on multiple plant species. How RPW8.2 activates defense remains uncharacterized. Here, we report that RPWS.2 interacts with the phytochrome-associated protein phosphatase type 2C （PAPP2C） in yeast and in planta as evidenced by co- immunoprecipitation and bimolecular fluorescence complementation assays. Down-regulation of PAPP2C by RNA interfer- ence （RNAi） in Col-0 plants lacking RPWS.2 leads to leaf spontaneous cell death and enhanced disease resistance to powdery mildew via the SA-dependent signaling pathway. Moreover, down-regulation of PAPP2C by RNAi in the RPW8.2 background results in strong HR-like cell death, which correlates with elevated RPWS.2 expression. We further demonstrate that hemagglutinin （HA）-tagged PAPP2C prepared from tobacco leaf cells transiently transformed with HA-PAPP2C possesses phosphatase activity. In addition, silencing a rice gene （Os04g0452000） homologous to PAPP2C also results in spontaneous cell death in rice. Combined, our results suggest that RPW8.2 is functionally connected with PAPP2C and that PAPP2C negatively regulates SA-dependent basal defense against powdery mildew in Arabidopsis.

关键词： Arabidopsis PAPP2C RPW8 protein phosphatase powdery mildew resistance SA-signaling.

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A numerical investigation of bubble dynamics based on the potential-flow theory

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Journal of Marine Science and Application 2006年第4期5卷 14-21页

作者： yao xiong-liang ZHANG A-man College of Shipbuilding Engineering Harbin Engineering University Harbin 150001 China

In this paper, the flow field is assumed to be inviscid, irrotational and incompressible, triangular elements are adopted to discretize the boundary of flow field, the boundary integral method is used to solve the flow field and the Mixed-Eulerian-Lagrangian method is applied to simulate the evolution of bubble. Three-dimensional smoothing method is used to smooth the bubble surface and the velocity potential to make the computing process more accurate and stable. In the analysis process, three-dimensional model simulates the dynamics of a bubble in the free field, gravitational field and near the rigid wall respectively, and the calculated results coincide well with the exact results and experimental data, which show that the algorithm and 3D model in this paper are of high accuracy. Calculation process indicates that bubble takes on strong non-linear under the combine effect of gravity and rigid wall.

关键词： bubble potential-flow theory boundary integral jet rigid wall three-dimensional

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近壁面气泡的运动规律研究

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物理学报 2008年第3期57卷 1662-1671页

作者：张阿漫姚熊亮哈尔滨工程大学船舶工程学院哈尔滨150001

基于势流假设,建立气泡与壁面耦合数值模型,运用边界积分法求解,并开发三维计算程序,计算值与实验值符合很好.从气泡与壁面相互作用的基本现象入手,基于开发的程序系统地研究了刚性壁面附近气泡的动力学特性,其中包括水平壁面及倾斜壁面... 详细信息

基于势流假设,建立气泡与壁面耦合数值模型,运用边界积分法求解,并开发三维计算程序,计算值与实验值符合很好.从气泡与壁面相互作用的基本现象入手,基于开发的程序系统地研究了刚性壁面附近气泡的动力学特性,其中包括水平壁面及倾斜壁面,研究壁面的Bjerknes效应与各特征参数之间的关系,并将各种工况的计算结果与基于Kelvin-impulse理论的Blake准则进行对比分析讨论,得出偏射流方向及壁面压力与气泡的特征参数有密切的关系,同时给出了Blake准则的适用范围.旨在为相关的近壁面气泡动态特性研究提供参考.

关键词：气泡壁面边界积分 Bjerknes效应动力学特性

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近自由面水下爆炸气泡的运动规律研究

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物理学报 2008年第1期57卷 339-353页

作者：张阿漫姚熊亮哈尔滨工程大学船舶工程学院哈尔滨150001

基于边界积分法建立水下爆炸气泡与自由面耦合数值模型;并开发相应的计算程序,计算值与实验值符合较好.从气泡与自由面相互作用的基本现象入手,运用开发的程序系统地研究了近自由面水下爆炸气泡的动态特性,包括近自由面气泡的环状回弹... 详细信息

基于边界积分法建立水下爆炸气泡与自由面耦合数值模型;并开发相应的计算程序,计算值与实验值符合较好.从气泡与自由面相互作用的基本现象入手,运用开发的程序系统地研究了近自由面水下爆炸气泡的动态特性,包括近自由面气泡的环状回弹及自由面的水冢现象;研究距离参数、浮力参数以及强度参数等特征参数与自由面Bjerknes效应之间的关系,总结相关规律,近自由面气泡的动态特性与这些特征参数有密切的关系.并在研究过程中,讨论了基于Kelvin-impulse理论的Blake准则的适用范围,并解释了Blake准则的失效的原因.旨在为相关的近自由面水下爆炸气泡动态特性研究提供参考.

关键词：气泡自由面环状水冢

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Enhancement of Frame-rate Using Digital Camera

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Semiconductor Photonics and Technology 2007年第1期13卷 80-82,88页

作者： LIU Wen-jing HUANG yao-xiong xiong Ping Institute of Biomedical Engineering Technology Jinan University Guangzhou 510632 CHN Chongqing Optoelectronics Research Institute Chongqing 400060 CHN

Frame rate is corresponding to the temporal resolution and the number of the pixels of the picture is corresponding to the spatial resolution. They are both very important for the researchers. The ideal image analysis and processing system should have high spatiotemporal characteristics, which is much expensive in practical use. A new method to enhance dynamic frame rate using multi-digital camera(DC) is proposed and the result shows that it could increase the frame rate effectively and decreases the cost in practical use.

关键词： frame rate temporal resolution digital camera

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