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Bear bile powder attenuates senecionine-induced hepatic sinusoidal obstruction syndrome in mice

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Chinese Journal of Natural Medicines 2022年第4期20卷 270-281页

作者： JIANG Kai-Yuan ZHANG Yi YE Xuan-Ling xiong fen CHEN Yan JIA Xia-Li ZHANG Yi-Xin YANG Li xiong Ai-Zhen WANG Zheng-Tao The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines Institute of Chinese Materia MedicaShanghai University of Traditional Chinese MedicineShanghai 201203China Shanghai R&D Center for Standardization of Traditional Chinese Medicines Shanghai 201203China Institute of Interdisciplinary Integrative Medicine Research Shanghai University of Traditional Chinese MedicineShanghai 201203China

Hepatic sinusoidal obstruction syndrome(HSOS)via exposure to pyrrolizidine alkaloids(PAs)is with high mortality and there is no effective treatment in *** bile powder(BBP)is a famous traditional animal drug for curing a variety of hepatobiliary diseases such as cholestasis,inflammation,and ***,we aim to evaluate the protective effect of BBP against HSOS induced by senecionine,a highly hepatotoxic PA *** results showed that BBP treatment protected mice from senecionine-induced HSOS dose-dependently,which was evident by improved liver histology including reduced infiltration of inflammatory cells and collagen positive cells,alleviated intrahepatic hemorrhage and hepatic sinusoidal endothelial cells,as well as decreased conventional serum liver function *** addition,BBP treatment lowered matrix metalloproteinase 9 and pyrrole-protein adducts,two well-known markers positively associated with the severity of PA-induced *** investigation showed that BBP treatment prevents the development of liver fibrosis by decreasing transforming growth factor beta and downstream fibrotic *** treatment also alleviated senecionine-induced liver inflammation and lowered the pro-inflammatory cytokines,in which taurours-odeoxycholic acid played an important ***’s more,BBP treatment also decreased the accumulation of hydrophobic bile acids,such as cholic acid,taurocholic acid,glycocholic acid,as *** concluded that BBP attenuates senecionine-induced HSOS in mice by repairing the bile acids homeostasis,preventing liver fibrosis,and alleviating liver *** present study helps to pave the way to therapeutic approaches of the treatment of PA-induced liver injury in clinics.

关键词： Hepatic sinusoidal obstruction syndrome Pyrrolizidine alkaloids Bear bile powder Liver disease Bile acids

Paravertebral block's effect on analgesia and inflammation in advanced gastric cancer patients undergoing transarterial chemoembolization and microwave ablation

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World Journal of Gastrointestinal Surgery 2024年第1期16卷 196-204页

作者： Ying-fen xiong Ben-Zhong Wei Yu-feng Wang Xiao-feng Li Cong Liu Department of Anesthesiology The First Affiliated Hospital of Nanchang UniversityNanchang 330006Jiangxi ProvinceChina Department of Anesthesiology Nanjing Gulou Hospital Group Yizheng HospitalYangzhou 211400Jiangsu ProvinceChina Nuclear Medicine Department Xuzhou Cancer HospitalXuzhou 221000Jiangsu ProvinceChina Department of Radiology Xuzhou Cancer HospitalXuzhou 221000Jiangsu ProvinceChina Department of Minimally Invasive Oncology Xuzhou New Health Hospital(Xuzhou Hospital Affiliated to Jiangsu University)Xuzhou 221000Jiangsu ProvinceChina

BACKGROUND Transarterial chemoembolization(TACE)combined with microwave ablation(MWA)is an effective treatment strategy for patients with advanced gastric cancer and liver ***,it may cause severe postoperative pain and inflammatory *** paravertebral block(PVB)is a regional anesthetic technique that provides analgesia to the thoracic and abdominal *** To evaluate the effect of PVB on postoperative analgesia and inflammatory response in patients undergoing TACE combined with MWA for advanced gastric cancer and liver *** Sixty patients were randomly divided into PVB and control *** PVB group received ultrasound-guided PVB with 0.375%ropivacaine preoperatively,whereas the control group received intravenous analgesia with *** primary outcome was the visual analog scale(VAS)score for pain at 6 h,12 h,24 h,and 48 h after the *** outcomes were the dose of sufentanil used,incidence of adverse events,and levels of inflammatory markers(white blood cell count,neutrophil percentage,C-reactive protein,and procalcitonin)before and after the *** The PVB group had significantly lower VAS scores at 6 h,12 h,24 h,and 48 h after the procedure compared with the control group(Pfentanil and a lower incidence of nausea,vomiting,and respiratory depression than did the control group(P<0.05).Compared with the control group,the PVB group had significantly lower levels of inflammatory markers 24 h and 48 h after the procedure(P<0.05).CONCLUSION PVB can effectively reduce postoperative pain and inflammatory responses and improve postoperative comfort and recovery in patients with advanced gastric cancer and liver metastasis treated with TACE combined with MWA.

关键词： Transarterial chemoembolization Microwave ablation Paravertebral block Visual analog scale Sufentanil Inflammatory markers

Secretory expression and characterization of a recombinant-deleted variant of human hepatocyte growth factor in Pichia pastoris

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World Journal of Gastroenterology 2005年第45期11卷 7097-7103页

作者： Zhi-Min Liu Hong-Liang Zhao Chong Xue Bing-Bing Deng Wei Zhang Xiang-Hua xiong Bing-fen Yang xue-Qin Yao Department of Microbiological Engineering Beijing instituteof Biotechnology 20 Dongdajie Street Fengtai District Beijing100071 China

AIM： To study the secretory expression of human hepatocyte growth factor （hdHGF） gene in Pichia pastoris. METHODS： The full-length gene of human cDNA encoding the deleted variant of hdHGF was cloned by RT-PCR and overlapping-fragment PCR technique using mRNA of human placenta as a template. The cloned hdHGF cDNA was inserted into the Escherichia coilyeast shuttle vector of pPIC9. The constructed plasmid, pPIC9-hdHGF, was transformed into the GSl15 cells of the methylotrophic yeast, P pastoris, using a chemical method. The Hut ＋ transformants were screened to obtain high-expression strains by the test and analysis of expressed products of shake-flask culture. A secretory form of rhdHGF was made with the aid of the leader peptide sequence of Saccharomyces cerevisiae α-factor. RESULTS： The expressed products, which showed a band of molecular mass of about 80 ku, were observed on 15% SDS-PAGE and identified by Western blotting and N-terminal amino acid sequencing. In the high cell density culture of 5 L fermentor by fed-batch culture protocol, the cell biomass was reached at approximately 135 g （DCW）/L. The productivity of secreted total supernant protein concentration attained a high-level expression of more than 8.0 g/L and the ratio of rhdHGF band area was about 12.3% of the total band area scanned by SDS-PAGE analysis, which estimated that the product of rhdHGF was 500-900 mg/***： The P pastoris system represents an attractive tool of generating large quantities of hdHGF for both research and industrial purposes.

关键词： Hepatocyte growth factor Pichia pastoris Secretory expressing

Research on the lightweight Application of Micro-foaming TPV Material in Glass Guide Assembly

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Research on the lightweight Application of Micro-foaming TPV...

Clinical application of combined detection of SARS-CoV-2-specific antibody and nucleic acid

2020中国汽车工程学会年会暨展览会

作者： Huang Shuo Wang Qiang Li Zhihuan xiong fen Gao Xiangda Dongfeng Motor Corporation Technical Center

The application of the microfoam TPV material in glass guide grooves had been *** showed that the performance of the microfoam material meets standard requirements,the product performance of the microfoam TPV glass guide grooves meets the design *** application meanwhile reduces the part weight,realizes the lightweight on such part.

关键词： microfoamed TPV glass guide groove lightweight

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World Journal of Clinical Cases 2020年第19期8卷 4360-4369页

作者： Qing-Bin Me ng Jing-Jing Peng Xin Wei Jia-Yao Yang Peng-Cheng Li Zi-Wei Qu Yong-fen xiong Guang-Jiang Wu Zhi-Min Hu Jian-Chun Yu Wen Su Department of Pulmonary and Critical Care Medicine and Department of Gastrointestinal Surgery Wuhan Integrated TCM and Western Medicine HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022Hubei ProvinceChina Department of Respiratory Medicine General Hospital of the Yangtze River ShippingWuhan 430015Hubei ProvinceChina Department of Gastrointestinal Surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430000Hubei ProvinceChina Department of Gastroenterology Wuhan Integrated TCM and Western Medicine HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022Hubei ProvinceChina Department of Transfusion Medicine Wuhan Integrated TCM and Western Medicine HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022Hubei ProvinceChina Department of Gastrointestinal Surgery Wuhan Integrated TCM and Western Medicine HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022Hubei ProvinceChina Department of Infection Management and Disease Control Beijing Shijitan HospitalCapital Medical UniversityBeijing 100038China Department of Clinical Microbiology and Molecular Biology Wuhan Integrated TCM and Western Medicine HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022Hubei ProvinceChina Department of General Surgery Peking Union Medicine College HospitalChinese Academy of Medical SciencesBeijing 100730China Department of Science and Education Wuhan Integrated TCM and Western Medicine HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022Hubei ProvinceChina

BACKGROUND The global outbreak of human severe acute respiratory syndrome coronavirus(SARS-CoV)-2 infection represents an urgent need for readily available,accurate and rapid diagnostic *** acid testing of respiratory tract specimens for SARS-CoV-2 is the current gold standard for diagnosis of coronavirus disease 2019(COVID-19).However,the diagnostic accuracy of reverse transcription polymerase chain reaction(RT-PCR)tests for detecting SARS-CoV-2 nucleic acid may be lower than *** detection of SARS-CoV-2-specific antibodies should be used as a serological non-invasive tool for the diagnosis and management of SARS-CoV-2 *** To investigate the diagnostic value of SARS-CoV-2 IgM/IgG and nucleic acid detection in *** We retrospectively analyzed 652 suspected COVID-19 patients,and 206 non-COVID-19 patients in Wuhan Integrated TCM and Western Medicine *** on SARS-CoV-2 nucleic acid tests and serum antibody tests were collected to investigate the diagnostic value of nucleic acid RT-PCR test kits and immunoglobulin(Ig)M/IgG antibody test *** j2 test was used to compare differences between categorical variables.A 95%confidence interval(CI)was provided by the Wilson score *** analyses were performed with IBM SPSS Statistics version 22.0(IBM Corp.,Armonk,NY,United States).RESULTS Of the 652 suspected COVID-19 patients,237(36.3%)had positive nucleic acid tests,311(47.7%)were positive for IgM,and 592(90.8%)were positive for *** was a significant difference in the positive detection rate between the IgM and IgG test groups(P<0.001).Using the RT-PCR results as a reference,the specificity,sensitivity,and accuracy of IgM/IgG combined tests for SARS-CoV-2 infection were 98.5%,95.8%,and 97.1%,*** the 415 suspected COVID-19 patients with negative nucleic acid test results,366 had positive IgM/IgG tests with a positive detection rate of 88.2%.CONCLUSION Our data indicate that serological IgM/IgG antibody co

关键词： SARS-CoV-2 COVID-19 Nucleic acid detection Immunoglobulin M Immunoglobulin G Diagnosis

An Efficient and Highly Stereocontrolled Synthesis of Coenzyme Q10 via a C5+C45 strategy

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An Efficient and Highly Stereocontrolled Synthesis of Coenzy...

中国化学会第四届有机化学学术会议

作者： xiong-Jie Yu, fen-Er Chen Department of Chemistry, Fudan University Shanghai, 200433

A practical and highly stereoselective synthesis of coenzyme Q10 has been accomplished in an overall yield of 31.3% starting from 3-methyl-5,6-dimethoxybenzoquinone (2). The construction of the isoprenyl group with (E)-configuration at C-2 position in ester 5 was realized via a coupling reaction of 7 with oxirane, Swern oxidation and Wittig olefination. Reduction of 5 in ethyl ether followed by bromination gave bromide 6 which was condensed

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我国对外贸易与经济增长关系的实证分析

嘉应学院学报 2012年第12期30卷 42-47页

作者：熊芬嘉应学院数学学院广东梅州514015

利用我国1978-2010年度数据,在VAR模型的基础上,运用协整检验和误差修正模型、格兰杰因果检验、脉冲响应分析和方差分解的方法对我国对外贸易与经济增长之间的关系进行实证分析。结果发现,我国经济增长大大促进了进出口贸易的发展,我国... 详细信息

利用我国1978-2010年度数据,在VAR模型的基础上,运用协整检验和误差修正模型、格兰杰因果检验、脉冲响应分析和方差分解的方法对我国对外贸易与经济增长之间的关系进行实证分析。结果发现,我国经济增长大大促进了进出口贸易的发展,我国对外贸易和经济增长之间也具有长期均衡关系。长期来看,出口对国民经济增长具有推动作用,我国是出口导向型经济;从短期来看,进口对经济增长又有促进作用。进口、出口是经济增长的Granger原因,进口增长也是出口增长的Granger原因,出口对经济增长的贡献度比进口更大。我国要实现进出口贸易平衡发展,从而促进经济健康发展。

关键词：对外贸易协整格兰杰因果检验脉冲响应函数方差分解

B超引导下留置针穿刺技术在增强CT穿刺困难患者中的应用效果

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中国医疗器械信息 2024年第17期30卷 134-136页

作者：熊芬江西省吉安市中心人民医院江西吉安343000

目的:探讨增强CT穿刺困难患者应用B超引导下留置针穿刺技术的效果。方法:回顾性选取2020年2月~2023年2月本院增强CT穿刺困难患者80例,依据穿刺方法分为B超引导下留置针穿刺技术组(B超引导组)、常规穿刺组,各40例。统计分析两组一次置管... 详细信息

目的:探讨增强CT穿刺困难患者应用B超引导下留置针穿刺技术的效果。方法:回顾性选取2020年2月~2023年2月本院增强CT穿刺困难患者80例,依据穿刺方法分为B超引导下留置针穿刺技术组(B超引导组)、常规穿刺组,各40例。统计分析两组一次置管成功情况、非计划拔管情况穿刺次数、操作时间、留置针留置时间、耗材费用、增强CT图像质量、不良反应发生情况、满意度。结果:B超引导组患者的一次置管成功率高于常规穿刺组(P<0.05),非计划拔管率低于常规穿刺组(P<0.05)。B超引导组患者的穿刺次数少于常规穿刺组(P<0.05),操作时间、留置针留置时间均短于常规穿刺组(P<0.05),耗材费用低于常规穿刺组(P<0.05)。B超引导组患者的增强CT图像质量优良率95.00%(38/40)高于常规穿刺组的75.00%(30/40),差异有统计学意义(χ^(2)=6.275,P<0.05)。B超引导组患者的不良反应发生率7.50%(3/40)低于常规穿刺组的25.00%(10/40),差异有统计学意义(χ^(2)=4.501,P<0.05)。B超引导组患者的满意度97.50%(39/40)高于常规穿刺组的80.00%(32/40),差异有统计学意义(χ^(2)=4.507,P<0.05)。结论:增强CT穿刺困难患者应用B超引导下留置针穿刺技术的效果较常规穿刺好。

关键词：增强CT 穿刺困难 B超引导留置针穿刺技术一次置管成功非计划拔管

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高职院校辅导员职业化问题与对策探赜

成才之路 2021年第33期 15-17页

作者：熊芬湖南铁路科技职业技术学院湖南株洲412000

辅导员职业化是通过统一的职业标准,构建价值共识、优化知识结构、制定育人规范、塑造职业形象,最终形成能助力辅导员素养提升、价值生成、自我奋斗的合力。在推进辅导员职业化的进程中,政府通过组织竞赛、职业培训、项目遴选、推优示... 详细信息

辅导员职业化是通过统一的职业标准,构建价值共识、优化知识结构、制定育人规范、塑造职业形象,最终形成能助力辅导员素养提升、价值生成、自我奋斗的合力。在推进辅导员职业化的进程中,政府通过组织竞赛、职业培训、项目遴选、推优示范等办法引导,院校依靠行政力量促进,但是辅导员个体追求职业化的动力相对滞后,在高职院校显得尤为突出。应推动平台建设,强化政策引领;重视名师打造,突出品牌效应;促进个体交流,唤醒互生共长;坚持内培外拓,开拓公共空间。

关键词：高职院校辅导员职业化立德树人能力培养