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Oral Immunization of Mice With Vaccine of Attenuated Salmonella typhimurium Expressing Helicobacter pylori Urease B Subunit

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Biomedical and Environmental Sciences 2005年第6期18卷 411-418页

作者： xing-LONG YANG WEN-CHAO lid.Wu-WEI YANG DONG ZHONG Yu-Hu LIU JING-DONG ZHANG JIAN-HUI JIANG SHAN-SHAN LI Department of Oncology Xijing Hospital The Fourth Military Medical University Xi＇an 710032 Shaanxi China Department of Oncology Affiliated Hospital of the Academy of Military Medical Sciences Beijing 100039 China Department of Gastroenterology Nanfang Hospital Guangzhou 510515 Guangdong China Department of Pathology University of Vermont 208 South Park Dr. Suite 2 Colchester VT05446 U.S.A. Department of Epidemiology the Fourth Military Medical University Xi＇an 710032 Shaanxi China

Objective To prepare the live recombinant vaccine of attenuated Salmonella typhimurium SL3261 expressing Helicobacterpylori （H. pylori） B subunit （UreB） and to determine whether it could be used as an oral vaccine against H. pylori infection. Methods Using genomic DNA of H. pylori Sydney strain （SS1） as template, the H. pylori UreB gene fragment was amplified by PCR and subcloned into the expression vector pTC01. The recombinant plasmid pTC01-UreB was then transferred into LBS000 to obtain modified forms, and further conversed into the attenuated Salmonella typhimurium SL3261 to obtain recombinant SL3261/pCT01-UreB as an oral immunization reagent, which was then used to orally immunize Balb/c mice twice at a three-week interval. Twelve weeks later, anti-UreB IgA antibodies in intestinal fluid and IgG antibodies in sera were determined by ELISA. The relating data in control groups （including body weight, gastric inflammation, etc.） were also collected. Results The sequencing analysis showed that the UreB gene fragment amplified by PCR was consistent with the sequence of the H. pylori UreB gene. The restriction enzyme digestion revealed that the correct pTC01-UreB was obtained. SDS-PAGE and Western blot showed that a 61KD protein was expressed in SL3261/pTC01-UreB, which could be recognized by anti-H, pylori UreB antiserum and was absent in the control containing only Salmonella typhimurium SL3261 strain. The multiple oral immunization with SL3261/pTC01-UreB could significantly induce H. pylori specific mucosal IgA response as well as serum IgG responses. IFN-T and IL-10 levels were significantly increased in SL3261/pTC01-UreB group, and no obvious side effect and change in gastric inflammation were observed. Conclusion The attenuated vaccine of Salmonella typhimurium expressing H. pylori UreB can be used as an oral vaccine against H. pylori infection.

关键词： Helicobacterpylori Urease B subunit Recombination Attenuated Salmonella typhimurium Oral vaccine

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Zero increase in peak discharge for sustainable development

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Frontiers of Environmental Science & Engineering 2017年第4期11卷 11-21页

作者： xing Fang Junqi Li Yongwei Gong Xiaoning Li Department of Civil Engineering Auburn University Auburn AL 36849-5337 USA Key Laboratory of Urban Stormwater System and Water Environment Ministry of Education Beijing University of Civil Engineering and Architecture Beijing 100044 China

For urban land development, some or all natural land uses （primarily pervious） are converted into impervious areas which lead to increases of runoff volume and peak discharge. Most of the developed countries require a zero increase in peak discharge for any land development, and the policy has been implemented for several decades. The policy of zero increase in peak discharge can be considered as historical and early stage for the low impact development （lid. and sustainable development, which is to maintain natural hydrological conditions by storing a part or all of additional runoff due to the development on site. The paper will discuss the policy, the policy implementation for individual projects and their impact on regional hydrology. The design rainfalls for sizing lid.facilities that are determined in 206 weather stations in USA are smaller than design rainfalls for sizing detention *** zero-increase policy links to financial responsibility and sustainability for construction of urban stormwater infrastructures and for reducing urban flooding. The policy was compared with current practices of urban development in China to shine the light for solving urban stormwater problems. The connections and differences among lid.practices, the zero-increase policy, and the flood control infrastructure were discussed. We promote and advocate the zero-increase policy on peak discharge for comprehensive stormwater management in China in addition to lid.

关键词： Stormwater management Detention basin Zero increase Peak discharge ' Sustainable development Design rainfall

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Theoretical and numerical study of hydraulic characteristics of orifice energy dissipator

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Water Science and Engineering 2010年第2期3卷 190-199页

作者： Ning HE Zhen-xing ZHAO Institute of High Energy Physics CAS Beijing 100049 R R. China College of Mechanics and Materials Hohai University Nanjing 210098 P. R. China

Different factors affecting the efficiency of the orifice energy dissipator were investigated based on a series of theoretical analyses and numerical simulations. The main factors investigated by dimension analysis were identified, including the Reynolds number （Re）, the ratio of the orifice diameter to the inner diameter of the pipe （ did ）, and the ratio of distances between orifices to the inner diameter of the pipe （ lid.）. Then, numerical simulations were conducted with a k-ε two-equation turbulence model. The calculation results show the following： Hydraulic characteristics change dramatically as flow passes through the orifice, with abruptly increasing velocity and turbulent energy, and decreasing pressure. The turbulent energy appears to be low in the middle and high near the pipe wall. For the energy dissipation setup with only one orifice, when Re is smaller than 105, the orifice energy dissipation coefficient K increases rapidly with the increase of Re. When Re is larger than l05, K gradually stabilizes. As diD increases, K and the length of the recirculation region L1 show similar variation patterns, which inversely vary with diD. The function curves can be approximated as straight lines. For the energy dissipation model with two orifices, because of different incoming flows at different orifices, the energy dissipation coefficient of the second orifice （K2） is smaller than that of the first. If lid.is less than 5, the K value of the lid.model, depending on the variation of/（2, increases with the spacing between two orifices L, and an orifice cannot fulfill its energy dissipation function. If lid.is greater than 5, K2 tends to be steady; thus, the K value of the lid.model gradually stabilizes. Then, the flow fully develops, and L has almost no impact on the value of K.

关键词： orifice energy dissipator theoretical analysis numerical simulation k-ε two-equation turbulent model hydraulic characteristics

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Applications of the CE/SE Scheme to Incompressible Viscous Flows in Two-Sided lid.Driven Square Cavities

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Chinese Physics Letters 2012年第8期29卷 176-180页

作者： YANG Duo-xing ZHANG De-Liang Institute of Crustal Dynamics Chinese Earthquake AdministrationBeijing 100085 LHD Institute of MechanicsChinese Academy of SciencesBeijing 100190

The spacetime conservation element-solution element(CE/SE)method is extended to two-dimensional incompressible viscous flow in a two-sided lid.driven square *** on the SIMPLE method concept,the preconditioned dual-time scheme is introduced for unsteady *** CE/SE-based code is valid.ted by simulating one-sided lid.driven cavity *** two-sided lid.driven square cavity problem involves several interesting characteristics being successfully predicted,including development of a pair of off-corner vortices and a free shear layer in the case of parallel wall motion,as well as the appearance of corner vortices for lower Reynolds numbers in the case of anti-parallel motion of the *** is found that both the Reynolds number and the direction of the moving walls affect the fluid flow in the cavity.

关键词： sided walls Reynolds

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A systematic review of the treatment of lower eyelid.retraction and our attempt of a dermal-orbicularis oculi suspension flap

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Chinese Journal of Plastic and Reconstructive Surgery 2022年第1期4卷 38-43页

作者： Yi Ding xing Huang Lin Lu Rui Jin Di Sun Jun Yang Xusong Luo Department of Plastic and Reconstructive Surgery Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200011China

Lower eyelid.retraction describes the inferior displacement of the lower eyelid.with or without inversion or *** on the causes of lower eyelid.retraction,we divided the forming factors of lower eyelid.retraction into three categories:(1)change in the balance of forces associated with the lower eyelid.margin;(2)excessive loss of lower eyelid.volume;(3)changes in the relative position of the eyeball and lower lid.*** this review,the corresponding treatment methods are elaborated on individually for the above three forming *** also reported,for the first time,a new treatment for lower eyelid.*** created a dermal-orbicularis oculi suspension flap on the lateral side of the canthus and suspended it upward and inward on the dense connective tissue over the lateral bony surface of the orbital *** longest follow-up time was one year,and the results were satisfactory.

关键词： Lower eyelid.retraction Dermal-orbicularis oculi suspension flap Orbicularis retaining ligament

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Biomaterials for tarsal plate reconstruction and our innovative work

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Chinese Journal of Plastic and Reconstructive Surgery 2021年第3期3卷 150-154页

作者： xing Huang Yi Ding Lin Lu Rui Jin Sun Di Jun Yang Xusong Luo Department of Plastic and Reconstructive Surgery Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200011China

Large tarsal plate defect reconstruction is one of the most challenging tasks for plastic *** on our practical work and literature review,the techniques and postoperative results of the preserved sclera,ear cartilage,and acellular dermal matrix(ADM)as tarsal plate substitutes were *** cases were *** 1 was a 22-year-old female who had total loss of the right lower *** cartilage(23 mm25 mm)was implanted under the expanded skin during the forehead skin-expanding procedure to form a skin-cartilageexpander capsule compound structure for lower eyelid.*** 2 was a 7-year-old boy with a unilateral divided *** lid.and conjunctiva of the lower eyelid.were invaded.A piece of ADM(12 mm4 mm)was used to repair the tarsal plate defect(12 mm3 mm).The conjunctiva of ADM was covered by *** orbicularis oculi myocutaneous flap was used for anterior lamella *** case 1,the compound tissue survived and supported the lower eyelid.the lid.margin was clear,and the structure was complete over a 1-year *** aesthetic appearance of the eyelid.improved after *** is the first clinical research on the expander capsule as eyelid.*** case 2,the conjunctiva completely covered the superior ADM,and the transplant merged with the surrounding tissues without complications after 5 *** preserved sclera has the longest application history as a tarsal plate ***,it should be preserved in an eye bank and transplanted with conjunctiva *** cartilage is an autologous tissue that requires conjunctival *** clinical practice was the first to prove the possibility of the expander capsule as eyelid.*** is feasible to prefabricate the skin-tarsal conjunctiva complex during the skin expansion *** ADM is a common clinical material with advantages of good biological histocompatibility,supportive strength,conjunctivalization,and not requir

关键词： Tarsal plate reconstruction Ear cartilage Preserved sclera Acellular dermal matrix Conjunctival repair Expander capsule

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基于GPU并行的改进SPH方法对粘性流场的模拟

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哈尔滨工程大学学报 2015年第8期36卷 1011-1018页

作者：金善勤郑兴段文洋哈尔滨工程大学船舶工程学院黑龙江哈尔滨150001

光滑粒子水动力学(SPH)方法对模拟破碎波问题有着良好的适应性。基于众核架构的GPU计算平台在加速SPH方法方面有着强大的优势。针对传统SPH方法计算效率低和计算精度差的问题,采用δ-SPH方法对腔内剪切流动、Poiseuille流动、Couette流... 详细信息

光滑粒子水动力学(SPH)方法对模拟破碎波问题有着良好的适应性。基于众核架构的GPU计算平台在加速SPH方法方面有着强大的优势。针对传统SPH方法计算效率低和计算精度差的问题,采用δ-SPH方法对腔内剪切流动、Poiseuille流动、Couette流动问题、孤立波砰击问题进行了模拟,并且提出一种基于粒子对的GPU并行计算方法。通过比较,得到不同边界处理方法对粘性流场模拟结果的影响规律,并且研究基于粒子对和单个粒子2种不同GPU并行计算方法,对比不同计算方法的精度和CPU时间。结果表明,采用粒子对的GPU并行方法可以使δ-SPH方法的最大加速比超过10。

关键词：光滑粒子流体动力学粒子对粘性流场孤立波砰击

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求解N-S方程的多边形网格与三角形网格对比

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工程热物理学报 2008年第7期29卷 1221-1223页

作者：王振兴徐明海中国石油大学(华东)储运与建筑工程学院热能工程系山东东营257061

本文介绍了基于边的数据结果离散对流扩散方程的方法,编制了适用于任意多边形单元网格的有限容积法计算程序,利用方腔自然对流、外掠后台阶和移动顶盖驱等算例考察了三角形网格与多边形网格在数值计算方面的性能差别。结果表明,采用三... 详细信息

本文介绍了基于边的数据结果离散对流扩散方程的方法,编制了适用于任意多边形单元网格的有限容积法计算程序,利用方腔自然对流、外掠后台阶和移动顶盖驱等算例考察了三角形网格与多边形网格在数值计算方面的性能差别。结果表明,采用三角形网格收敛较慢且不容易获得网格无关的解,而采用与三角形网格对偶的多边形网格则收敛较快且易获得网格无关的解。

关键词：非结构化网格多边形网格 N-S方程有限容积

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中低雷诺数流动直接数值模拟的算法比较（英文）

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船舶力学 2017年第6期21卷 651-660页

作者：王建春吴乘胜王星中国船舶科学研究中心江苏无锡214082

文章采用不同的算法对中低雷诺数方腔驱动流动进行了直接数值模拟,所用算法分别是人工压缩方法、SIMPLE算法以及PISO算法。三种算法均采用有限体积法基于交错网格技术离散N-S方程,时间项采用全隐格式离散,对流项采用QUICK格式离散,并将... 详细信息

文章采用不同的算法对中低雷诺数方腔驱动流动进行了直接数值模拟,所用算法分别是人工压缩方法、SIMPLE算法以及PISO算法。三种算法均采用有限体积法基于交错网格技术离散N-S方程,时间项采用全隐格式离散,对流项采用QUICK格式离散,并将它们得到结果与Ghia发表的基准解进行了比对。文中分析了在同样的收敛条件下,不同算法之间的稳定性,收敛速率以及准确性的差异,发现PISO算法在较低雷诺数Re=400和Re=1 000情况下最准确,而人工压缩算法在雷诺数为5 000时最准确,在所有计算的不同Re数条件下,发现人工压缩法达到收敛所需时间都是最少的,这可以使它成为中低雷诺数下研究直接数值模拟最好的算法之一。

关键词： SIMPLE PISO 人工压缩方法 DNS 准确性收敛速度稳定性

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朱蔚荣所藏“凤江炉”炉身图像考释

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艺术百家 2015年第5期31卷 148-155页

作者：赵兴勤赵韡江苏师范大学文学院江苏徐州221116

徐州朱蔚荣先生珍藏的明代手炉,铜质匀净,炉盖严密,历久而不失光泽,更兼刀工技法纯熟,线条舒展,纹饰精美。从款识、用料及雕工来看,当为著名匠人王凤江的作品。王凤江生活于明代中后叶,万历年间已成名,或是嘉靖年间生人。朱藏"凤江炉",... 详细信息

徐州朱蔚荣先生珍藏的明代手炉,铜质匀净,炉盖严密,历久而不失光泽,更兼刀工技法纯熟,线条舒展,纹饰精美。从款识、用料及雕工来看,当为著名匠人王凤江的作品。王凤江生活于明代中后叶,万历年间已成名,或是嘉靖年间生人。朱藏"凤江炉",炉身两面皆有人物图案,且神态各异,栩栩如生。经细致考察,这两幅图像分别表现的,殆即汪廷讷所撰《狮吼记》第九出《奇妒》、第十六出《顶灯》之相关场景。这一重要发现,对明代戏曲演出形态、剧目之传播与接受等研究均很有帮助,可补目前学者论述之未逮,并启示研究者,于传统的文本文献研究和常见的戏曲专属文物(如古戏台等)研究之外,还应关注业已融入古代日常生活的某些戏曲元素(如剪纸、年画、刺绣、瓷器、铜器、家具、门窗、瓦当等),此或为古代戏曲研究拓展出一条新的路径。

关键词：戏曲艺术戏曲文物王凤江凤江炉图像考释《狮吼记》

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