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Isolation and Structural Identification of Herbicidal Active Substance from Root of Flaveria bident(L.) Kuntze

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Journal of Integrative Agriculture 2014年第4期13卷 804-810页

作者： HUO jing-qian xing ji-hong ZHANG Li-hui KANG Zhan-hai ZHANG jin-lin College of Plant Protection Agricultural University of Hebei College of Life Sciences Agricultural University of Hebei

In order to understand the composition and structure of herbicidal active substance from the root of Flaveria bidentis （L.） Kuntze, the isolation and structural identification were researched in this paper. The crude extract from the root ofF. bidentis （L.） Kuntze was extracted by petroleum ether, ethyl acetate, and water saturation of n-butyl alcohol, respectively, and the extraction fluid was separated by using the method of TLC, then the main fraction was separated by HPLC, and the structure of the herbicidal active substance was analyzed by LC-MS, elemental analysis and ~H-NMR. The results showed that the petroleum extraction had the strongest herbicidal activity, and the purple blue stripe separated by TLC had the strongest effect on Digitaria sanguinalis. The herbicidal active substance was identified as ct-terthienyl according to the data of LC-MS, elemental analysis and 1H-NMR.

关键词： Flaveria bidentis (L.) Kuntze herbicidal active substance isolation structure identification

Cloning One CIPK Gene from a Thermo-Sensitive Genic Self-Incompatible Line in Maize Expressing Under Different Temperatures

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Agricultural Sciences in China 2011年第6期10卷 813-819页

作者： LIN xing-e ji hong-qing NIU Jun-hai HU Yan-min FU Zhong-jun LIU Zong-hua TANG ji-hua College of Agronomy Henan Agricultural University Zhengzhou 450002 P.R.China

A calcineurin B-like protein-interacting protein kinases （CIPK） gene was cloned （EU 424058） from a thermo-sensitive genic self-incompatibility （TSGI） line, HE97, in maize, which was selected for suppression subtractive hybridization （SSH） library of the self-incompatible and self-compatible silks of the TSGI line under different temperatures. The full-length cDNA of the candidate CIPK-like gene consisted of 1 918 nucleotides and contained a 1 332-bp open reading frame in maize. Real-time PCR indicated that the candidate CIPK-like gene was highly expressed in the self-incompatible pollen of the TGSI line, and promoted elevated levels of calcium （Ca2＋）. High Ca2＋ concentrations in the pollen strongly inhibit pollen tube formation in silk of the same plant, thus inducing self-incompatibility （SI） under high temperature. These results implied that the CIPKs-like gene would play an important role in the regulation of HE97 characteristics under different temperatures, perhaps acting as a secondary messenger in the expression of SI in the TGSI line in maize.

关键词： CIPK gene cloning expression temperature maize

BcSDR1 is involved in regulation of glucose transport and cAMP and MAPK signaling pathways in Botrytis cinerea

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Journal of Integrative Agriculture 2022年第9期21卷 2628-2640页

作者： SI He-long ZHANG Kang LI Bai YUAN Xue-mei ZANG jin-ping CAO hong-zhe xing ji-hong DONG jin-gao State Key Laboratory of North China Crop Improvement and Regulation Hebei Agricultural UniversityBaoding 071000P.R.China Hebei Key Laboratory of Plant Physiology and Molecular Pathology Hebei Agricultural UniversityBaoding 071000P.R.China

Botrytis cinerea is a typical necrotrophic pathogenic fungus that causes severe diseases in a wide range of plant species, leading to significant economic losses. Our previous study showed that BcSDR1 positively regulates growth,development, and pathogenicity of B. cinerea. However, the regulation mechanism of BcSDR1 and the relationship between BcSDR1 and cAMP and MAPK signaling pathways are not well understood. In this study, transcriptome data showed that BcSDR1 is involved in glucose transmembrane transport, signal transduction, secondary metabolism, and other biological processes. BcSDR1 mutant(BCt41) showed remarkably weak sensitivity to cAMP and MAPK signaling pathways specific inhibitors, SQ22536 and U0126, and significantly decreased cAMP content. The key genes of cAMP and MAPK signaling pathways, BcGB1, BcBTP1, BcBOS1, BcRAS1, and BcBMP3 were significantly upregulated,whereas BcPLC1, BcBCG1, BcCDC4, BcSAK1, BcATF1, and BcBAP1 were significantly downregulated(P<0.05).BcSDR1 was obviously upregulated in BcBCG2, BcBCG3, BcPKA1, and BcPKAR RNA interference(RNAi) mutants, but significantly downregulated in BcPKA2, BcBMP1, and BcBMP3 RNAi mutants. Thus, BcBCG2, BcBCG3, BcPKA1, and BcPKAR negatively regulate BcSDR1 expression, whereas BcPKA2, BcBMP1, and BcBMP3 positively regulate BcSDR1expression.

关键词： Botrytis cinerea BcSDR1 glucose transmembrane transport cAMP signaling pathway MAPK signaling pathway

Identification and expression analysis of sugar transporter family genes reveal the role of ZmSTP2 and ZmSTP20 in maize disease resistance

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Journal of Integrative Agriculture 2023年第11期22卷 3458-3473页

作者： MA Yu-xin ZHOU Zhi-jun CAO hong-zhe ZHOU Fan SI He-long ZANG jin-ping xing ji-hong ZHANG Kang DONG jin-gao State Key Laboratory of North China Crop Improvement and Regulation Hebei Agricultural UniversityBaoding 071000P.R.China Hebei Key Laboratory of Plant Physiology and Molecular Pathology Hebei Agricultural UniversityBaoding 071000P.R.China Hebei Bioinformatic Utilization and Technological Innovation Center for Agricultural Microbes Hebei Agricultural UniversityBaoding 071000P.R.China Experimental Training Center of Hebei Agricultural University Hebei Agricultural UniversityBaoding 071000P.R.China

Sugar is an indispensable source of energy for plant growth and development, and it requires the participation of sugar transporter proteins(STPs) for crossing the hydrophobic barrier in plants. Here, we systematically identified the genes encoding sugar transporters in the genome of maize(Zea mays L.), analyzed their expression patterns under different conditions, and determined their functions in disease resistance. The results showed that the mazie sugar transporter family contained 24 members, all of which were predicted to be distributed on the cell membrane and had a highly conserved transmembrane transport domain. The tissue-specific expression of the maize sugar transporter genes was analyzed, and the expression level of these genes was found to be significantly different in different tissues. The analysis of biotic and abiotic stress data showed that the expression levels of the sugar transporter genes changed significantly under different stress factors. The expression levels of Zm STP2 and Zm STP20 continued to increase following Fusarium graminearum infection. By performing disease resistance analysis of zmstp2 and zmstp20 mutants, we found that after inoculation with Cochliobolus carbonum, Setosphaeria turcica, Cochliobolus heterostrophus, and F. graminearum, the lesion area of the mutants was significantly higher than that of the wild-type B73 plant. In this study, the genes encoding sugar transporters in maize were systematically identified and analyzed at the whole genome level. The expression patterns of the sugar transporter-encoding genes in different tissues of maize and under biotic and abiotic stresses were revealed, which laid an important theoretical foundation for further elucidation of their functions.

关键词： maize sugar transporter gene expression disease resistance

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玉米大斑病菌菌丝蛋白单克隆抗体制备

植物病理学报 2006年第2期36卷 174-176页

作者：邢继红董金皋河北农业大学真菌毒素实验室保定071001

人们在研究玉米大斑病菌与寄主互作的模式时，推测病菌可能的致病机制是大斑病菌产生的HT-毒素先与玉米细胞膜受体蛋白（结合蛋白）结合，进而引发寄主的生理生化过程并导致表型的病变。基于HT-毒素为低分子量小分子化合物，具体的化学... 详细信息

人们在研究玉米大斑病菌与寄主互作的模式时，推测病菌可能的致病机制是大斑病菌产生的HT-毒素先与玉米细胞膜受体蛋白（结合蛋白）结合，进而引发寄主的生理生化过程并导致表型的病变。基于HT-毒素为低分子量小分子化合物，具体的化学结构尚不清楚，因此研究HT-毒素的结合蛋白和作用位点存在很大的困难。本试验从玉米大斑病菌的菌丝蛋向着手，制备玉米大斑病菌菌丝蛋白的单克隆抗体，以期获得与HT-毒素反应的单抗株，为进一步研究毒素的结合蛋白及作用位点奠定基础。[第一段]

关键词：玉米大斑病菌单克隆抗体菌丝蛋白抗体制备 HT-毒素结合蛋白小分子化合物受体蛋白致病机制生化过程

我国新一代信息技术产业自主创新能力评价研究

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华东经济管理 2017年第3期31卷 100-104页

作者：邢纪红龚惠群东南大学经济管理学院江苏南京210096 南京信息工程大学经济管理学院江苏南京210044

文章以我国新一代信息技术产业为研究对象,从自主研发的活动能力、创新成果的科研产出能力以及商业产出能力等三个方面构建了产业自主创新能力的评价指标体系。运用结构方程模型建立评价模型,并以我国新一代信息技术产业相关18个子行业2... 详细信息

文章以我国新一代信息技术产业为研究对象,从自主研发的活动能力、创新成果的科研产出能力以及商业产出能力等三个方面构建了产业自主创新能力的评价指标体系。运用结构方程模型建立评价模型,并以我国新一代信息技术产业相关18个子行业2009-2015年的面板数据为研究样本,对自主创新能力评价指标的合理性进行评价和实证分析。

关键词：新一代信息技术产业自主创新能力结构方程模型评价