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Establishment of a Sandwich ELISA Method for Detection of Vascular Endothelial Growth Factor in Serum Samples of Hepatocellular Carcinoma Patients

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biomedical and Environmental Sciences 2008年第1期21卷 69-74页

作者： biNG SHAN CHEN GAO JIAN-MING CHEN xin-yu bi BAO-yuN ZHANG YAN GUO CHEN-FANG DONG RUN AN QI SHI JING-QUN HU PING ZHAO JUN HAN XIAO-PING DONG State Key Laboratory for Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 100052 China Department of Abdominal Surgery Cancer Hospital Chinese Academy of Medical Sciences Beijing 100021 China

Objective To establish a sandwich ELISA method for detecting vascular endothelial growth factor （VEGF） in sera of population and the patients with hepatocellular carcinoma （HCC）. Methods Full length and two truncated human VEGF cDNA sequences were amplified from a commercial plasmid pBLAST49-hVEGF by PCR and inserted into the prokaryotic-expression plasmid pET-32a or pGEX-2T. Various VEGF proteins were expressed and purified from E. coli in His-Trx or GST fusion forms. The specific VEGF antibodies were elicited in experimental rabbits and mice by immunization of the full length VEGF fusion protein His-Trx-VEGF1-165. After purification of antibodies with chromatograph of Protein G, a sandwich ELISA technique was established. Serum VEGF levels were evaluated in 229 adults and 291 HCC patients. Results SDS-PAGE displayed that the molecular weights of the expressed full length （His-Trx-VEGF1-165）, N-terminal （His-Trx-VEGF1-100） and C-terminal （GST-VEGF100-165） human VEGF fusion proteins were about 38KD, 31KD, and 33KD, respectively. Western blots confirmed that the prepared antisera were able to recognize both prokaryoticly and eukaryoticly expressed recombinant VEGF proteins. Assays of serially diluted His-Trx-VEGF1-100 by the established sandwich ELISA method showed that the linear range of the standard curve was 0.625-320 ng/mL, with the squared correlation coefficient R^2=0.991. Screening of a serum panel containing 291 serum samples of HCC patients and 229 health adults revealed that the average VEGF level in HCC patients was higher than that in healthy controls, with a statically significant difference. Conclusion The established sandwich ELISA reflects the level of serum VEGF and provide scientific basis for screening metastasis and recurrence of HCC using serum VEGF as an index.

关键词： Hepatocellular carcinoma Vascular endothelial growth factor Enzyme-linked immunosorbent assay

Clinicopathological and Immunohistochemical Study of Low-Grade Myofibroblastic Sarcoma of the Liver One Case Report

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Clinical oncology and cancer researeh 2011年第4期8卷 250-253页

作者： Yi FANG Tao YAN xin-yu bi Hong-tu ZHANG Jian-guo ZHOU Zhen HUANG yu-quan XIE Ping ZHAO Jian-qiang CAI Department of Abdominal Surgery CancerHospital Chinese Academy of MedicalScience & Peking Union Medical CollegeBeijing China Department of Pathology Cancer HospitalChinese Academy of Medical Science &Peking Union Medical College Beijing China

Low-grade myofibroblastic sarcoma is a recently characterized tumor showing features of myofibroblastic differen-tiation that is part of the spectrum of malignant mesenchymal tumors. This extremely rare type of tumor occurs most commonly in superficial locations. The case we describe herein is that of a 60- year-old man with two large hepatic masses. The patient＇s tumor was removed radically through an incision due to the inconclusive imaging findings. Follow-up computed tomography showed no recurrence and metastasis after 37 months, suggesting that enucleation was adequate for tumor eradication. To our knowl-edge, this is the first reported case of low-grade myofibroblastic sarcoma in the liver in the English language literature.

关键词： myofibroblastic sarcoma liver soft-tissue neoplasms.

Risk Factors for Lymph Node Metastasis and Endoscopic Treatment Strategies of Undifferentiated Early Gastric Cancer

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Clinical oncology and cancer researeh 2011年第3期8卷 181-184页

作者： Yi FANG Tao YAN Ji-dong GAO xin-yu bi Hong ZHAO Hai-tao ZHOU Zhen HUANG Jian-qiang CAI Department of Abdominal Surgery Cancer Hospital Chinese Academy of Medical Sciences Beijing 100021 China

OBJECTIVE To discuss the effects of clinico-pathological features on lymph node metastasis （LNM） in undifferentiated EGC （early gastric cancer）, as well as identify the appropriate medical management. METHODS From January 1999 to June 2011, 352 patients were treated for undifferentiated EGC in our hospital. All patients had undergone gastrectomy with regional lymphadenectomy. We used univariate and multivariate associated with lymph node entiated EGC. analyses to determine the features metastasis in patients with undiffer- RESULTS Signet ring cell carcinoma （SRC） was more common in patients with undifferentiated EGC than other undifferentiated carcinoma （UDC）. SRC had a tendency to be confined to the mucosa, with a smaller size than other UDC. The incidence of LNM for SRC was lower than that for other UDC. Multivariate analysis showed that LNM was associated with the sex, tumor size, depth of invasion, lymphovascular invasion, and histological type. CONCLUSION Complete endoscopic resection is suitable for SRC- type intramucosal EGC, which is less than 2 cm in diameter without lymphovascular invasion in the postoperative histological examination.

关键词： undifferentiated early gastric cancer endoscopic submucosal dissection lymph node metastasis

Excision Repair Cross-complementation Group 1 is a Prognostic biomarker in Patients with Colorectal Cancer Receiving Chemotherapy

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Chinese Medical Journal 2016年第5期129卷 586-593页

作者： Mu-xing Li xin-yu bi Hong Zhao Zhen Huang yue Han Dong-bin Zhao Jian-Jun Zhao Jian-Qiang Cai Department of Abdominal Surgical Oncology Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100021 China Department of Interventional Therapies Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100021 China

Background：Conflicting results about the association between expression level of excision repair cross-complementation group 1 （ERCC1） and clinical outcome in patients with colorectal cancer （CRC） receiving chemotherapy have been ***,we searched the available articles and performed the meta-analysis to elucidate the prognostic role of ERCC1 expression in patients with ***：A thorough literature search using PubMed （Medline）,Embase,Cochrane Library,Web of Science databases,and Chinese Science Citation Database was conducted to obtain the relevant *** hazard ratios （HRs） or odds ratios （ORs） with 95% confidence intervals （CIs） were calculated to estimate the ***：A total of 11 studies were finally enrolled in this *** with patients with lower ERCC1 expression,patients with higher ERCC1 expression tended to have unfavorable overall survival （OS）（HR =2.325,95% CI：1.720-3.143,P 〈 0.001）,progression-free survival （PFS）（HR =1.917,95% CI：1.366-2.691,P 〈 0.001） and poor response to chemotherapy （OR =0.491,95% CI：0.243-0.990,P =0.047）.Subgroup analyses by treatment setting,ethnicity,HR extraction,detection methods,survival analysis,and study design demonstrated that our results were ***：ERCC1 expression may be taken as an effective prognostic factor predicting the response to chemotherapy,OS,and *** studies with better study design and longer follow-up are warranted in order to gain a deeper understanding of ERCC 1＇s prognostic value.

关键词： Colorectal Cancer: Excision Repair Cross-complementation Group I Prognosis

Evaluation of Eight Different Clinical Staging Systems Associated with Overall Survival of Chinese Patients with Hepatocellular Carcinoma

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Chinese Medical Journal 2015年第3期128卷 316-321页

作者： Jian-Jun Zhao Tao Yan Hong Zhao Jian-Guo Zhou Zhen Huang Ye-Fan Zhang yuan Li Zhi-yu Li xin-yu bi Jian-Qiang Cai Department of Abdominal Surgery Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100021 China Department of Anesthesiology Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100021 China

Background： Hepatocellular carcinoma （HCC） is a common cancer in China, an area of high hepatitis B virus （HBV） infection. Although several staging systems are available, there is no consensus on the best classification to use because multiple factors, such as etiology, clinical treatment and populations could affect the survival of HCC patients. Methods： This study analyzed 743 HBV-related Chinese HCC patients who received surgery first and evaluated the predictive values of eight different commonly used staging systems in the clinic. Results： The overall 1-, 3-, 5-year survival rates and a median survival were 91.5%, 70.3%, 55.3% and 72 months respectively. Barcelona Clinic Liver Cancer （BCLC） staging systems had the best stratification ability and showed the lowest Akaike information criterion （AIC） values （2896.577）, followed by tumor-node-metastasis Th （TNM 7^th）（AIC = 2899.980）, TNM 6th （AIC = 2902.17）, Japan integrated staging score （A1C = 2918.085）, Tokyo （AIC = 2938.822）, Cancer of the Liver Italian Program score （AIC = 2941.950）, Chinese University Prognostic Index grade （AIC = 2962.027）, and Okuda （AIC = 2979.389）. Conclusions： BCLC staging system is a better staging model for HBV infection patients with HCC in Chinese population among the eight currently used staging systems. These identifications afford a large group of Chinese HCC patients with HBV infection and could be helpful to design a new staging system for a certain population.

关键词： Chinese Population Clinical Staging Ssystem Hepatocellular Carcinoma Survival

Prognostic Analysis of 102 Patients with Synchronous Colorectal Cancer and Liver Metastases Treated with Simultaneous Resection

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Chinese Medical Journal 2017年第11期130卷 1283-1289页

作者： Rui Mao Xiao Chen Jian-Jun Zhao xin-yu bi Zhi-yu Li Jian-Guo Zhou Hong Zhao Zhen Huang Yong-Kun Sun Jian-Qiang Cai Department of Hepatobiliary Surgery National Cancer Center/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100021 China Department of Medical Oncology National Cancer Center/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100021 China

Background： The liver is the most common site for colorectal cancer （CRC） metastases. Their removal is a critical and challenging aspect of CRC treatment. We investigated the prognosis and risk factors of patients with CRC and liver metastases （CRCLM） who underwent simultaneous resections for both lesions. Methods： From January 2009 to August 2016, 102 patients with CRCLM received simultaneous resections of CRCLM at our hospital. We retrospectively analyzed their clinical data and analyzed their outcomes. Overall survival （OS） and disease-free survival （DFS） were examined by Kaplan-Meier and log-rank methods. Results： Median follow-up time was 22.7 months： no perioperative death or serious complications were observed. Median OS was 55.5 months： postoperative OS rates were l-year： 93.8%, 3-year： 60.7%, and 5-year： 46.4%. Median DFS was 9.0 months; postoperative DFS rates were l-year： 43.1%, 3-year： 23.0%, and 5-year 21.1%. Independent risk factors found in multivariate analysis included carcinoembryonic antigen 〉100 ng/ml, no adjuvant chemotherapy, tumor thrombus in liver metastases, and bilobar liver metastases for OS; age 〉60 years, no adjuvant chenlotherapy, multiple metastases, and largest diameter ≥3 cm for DFS. Conclusions： Simultaneous surgical resection is a safe and effective treatment for patients with synchronous CRC LM. The main prognostic factors are pathological characteristics of liver metastases and whether standard adjuvant chemotherapy is performed.

关键词： Colorectal Cancer Liver Metastases Prognosis Simultaneous Resection

Targeting TrkB–PSD-95 coupling to mitigate neurological disorders

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Neural Regeneration Research 2025年第3期20卷 715-724页

作者： xin Yang yu-Wen Alvin Huang John Marshall Department of Molecular biology Cell Biology and BiochemistryBrown UniversityProvidenceRIUSA Department of Neurology Warren Alpert Medical School of Brown UniversityProvidenceRIUSA Center for Translational Neuroscience Robert J.and Nancy D.Carney Institute for Brain Science and Brown Institute for Translational ScienceBrown UniversityProvidenceRIUSA

Tropomyosin receptor kinase B(TrkB)signaling plays a pivotal role in dendritic growth and dendritic spine formation to promote learning and *** activity-dependent release of brain-derived neurotrophic factor at synapses binds to pre-or postsynaptic TrkB resulting in the strengthening of synapses,reflected by long-term ***,the association of postsynaptic density protein-95 with TrkB enhances phospholipase Cγ-Ca^(2+)/calmodulin-dependent protein kinaseⅡand phosphatidylinositol 3-kinase-mechanistic target of rapamycin signaling required for long-term *** this review,we discuss TrkB-postsynaptic density protein-95 coupling as a promising strategy to magnify brain-derived neurotrophic factor signaling towards the development of novel therapeutics for specific neurological disorders.A reduction of TrkB signaling has been observed in neurodegenerative disorders,such as Alzheimer's disease and Huntington's disease,and enhancement of postsynaptic density protein-95 association with TrkB signaling could mitigate the observed deficiency of neuronal connectivity in schizophrenia and *** with brain-derived neurotrophic factor is problematic,due to poor pharmacokinetics,low brain penetration,and side effects resulting from activation of the p75 neurotrophin receptor or the truncated TrkB.T1 *** TrkB agonists and antibodies that activate TrkB are being intensively investigated,they cannot distinguish the multiple human TrkB splicing isoforms or cell type-specific *** TrkB–postsynaptic density protein-95 coupling provides an alternative approach to specifically boost TrkB signaling at localized synaptic sites versus global stimulation that risks many adverse side effects.

关键词： Angelman syndrome autism brain-derived neurotrophic factor depression neurodegenerative disorder neurodevelopmental disorder postsynaptic density protein-95 synaptic plasticity TrkB

Visible-light-responsive homologous heterostructure of oxynitrides constructed to boost charge separation and water splitting

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Science China Chemistry 2025年第1期68卷 186-191页

作者： Hai Zou yunfeng Bao Xueshang xin yu Qi Tengfeng Xie Fuxiang Zhang State Key Laboratory of Catalysis Dalian National Laboratory for Clean Energy Dalian Institute of Chemical Physics Chinese Academy of Sciences School of Chemistry and Chemical Engineering Liaoning Normal University College of Chemistry Jilin University

Constructing type II heterostructures represents a widely recognized strategy for enhancing charge separation and photocatalytic efficiency,but it remains challenging to fabricate homologous heterostructures based on visible-light-responsive semiconductors,especially for popularly investigated （oxy）nitrides with low thermal ***,we synthesized a homologous heterostructure （denoted as YTON＿H） based on the oxynitrides of YTaO4-xNyand YTa *** heterostructure exhibits type II band structure alignment and intimate interface achieved through a magnesium （Mg） powder-assisted nitridation *** with widely reported heterologous heterostructures,the as-obtained YTON＿H homologous heterostructure could similarly exhibit enhanced charge separation as well as photocatalytic water splitting performance with respect to its counterparts(YTaO4-xNyand YTaON2).Meanwhile,detailed structural characterizations and spectroscopy analysis have been made to confirm the formation of heterostructure and promotion of spatial charge separation.

关键词： charge separation heterostructure oxynitride photocatalysis visible light

Vector meson's spin alignments in high energy reactions

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 5-20页

作者： Jin-Hui Chen Zuo-Tang Liang yu-Gang Ma xin-Li Sheng Qun Wang Key Laboratory of Nuclear Physics and Ion-beam Application (MoE) Institute of Modern PhysicsFudan University Institute of Frontier and Interdisciplinary Science Key Laboratory of Particle Physics and Particle Irradiation (MOE)Shandong University INFN-Firenze Department of Modern Physics University of Science and Technology of China School of Mechanics and Physics Anhui University of Science and Technology

The global spin alignment of vector mesons has been observed by the STAR collaboration at the Relativistic Heavy Ion Collider （RHIC） at Brookhaven National Laboratory （BNL）.It provides a unique opportunity to probe the correlation between the polarized quark and antiquark in the strongly coupled quark-gluon plasma （sQGP） produced in relativistic heavy ion collisions,opening a new window to explore the properties of s *** addition,spin alignments of vector mesons have also been observed in other high-energy particle collisions such as e+e-annihilations at high energies where hadron production is dominated by quark fragmentation *** results obtained are quite different from those obtained in heavy ion collisions where quark coalescence/combination mechanism dominates suggesting strong dependence on hadronization *** comprehensive studies in different hadronization processes are *** this article,we present a brief review of theoretical and experimental advances in the study of vector meson’s spin alignments in a variety of high-energy particle collisions,with emphasis on hadronization mechanisms.

关键词： vector meson spin alignment global polarization relativistic heavy ion collisions fragmentation