检索结果-南通市图书馆

Agricultural Sciences in China 2011年第4期10卷 526-533页

作者： LIU Hong-xia xin zhi-yong ZHANG Zeng-yan National Key Facility for Crop Gene Resources and Genetic Improvement~Institute of Crop Sciences Chinese Academy of AgriculturalSciences Beijing 100081 P.R. China

In this study, the changes in activities of peroxidase （POD）, glutathione S-transferase （GST）, catalase （CAT）, and superoxide distmuase （SOD） in leaves of four wheat cultivars, including resistant cultivars Shannong 0431 and Shanhongmai, a mildsusceptible cultivar Lumai 21, and a susceptible cultivar Wenmai 6, were investigated to explore if these antioxidantrelated enzymes were involved in wheat defense response to Rhizoctonia cerealis attack. Results showed that the activities of these enzymes in different wheat cultivars varied greatly after challenging with R. cerealis. The POD activities in resistant cultivars Shannong 0431 and Shanhongmai increased much quicker after the inoculation and displayed much higher activity than that in susceptible cultivar Wenmai 6. In contrast, POD activity showed a slow accumulation and a delayed peak time. The activities of GST and CAT in Shannong 0431 and Lumai 21 increased obviously within 48 h post inoculation （hpi）, whereas SOD activity decreased dramatically within 24 hpi in Shannong 0431 and 24-48 hpi in Lumai 21. In susceptible cultivar Wenmai 6, the SOD and CAT activities didn’t change obviously, whereas the activity of GST was decreased. The activities of CAT and GST in Shanhongmai did not show significant difference compared with those in Wenmai 6. Results suggested that POD activity may be positively associated with wheat defense to R. cerealis.

关键词： wheat Rhizoctonia cerealis antioxidant-related enzymes peroxidase

Characterization and Molecular Mapping of a Stripe Rust Resistance Gene in Synthetic Wheat CI110

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2012年第4期11卷 521-527页

作者： REN Qiang LIU Hui-juan ZHANG Zeng-yan FENG Jing XU Shi-chang PU Zong-jun xin zhi-yong The National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops Ministry of Agriculture/Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 P.R.China Crop Research Institute Sichun Academy of Agricultural Sciences Chengdu 610066 P.R. China

Stripe rust,caused by Puccinia striiformis ***（Pst）,is one of the most destructive diseases of wheat（Triticum aestivum L.）.To diversify stripe rust-resistant resources for wheat breeding programs,a CIMMYT synthetic wheat line CI110 was identified to be resistant to 28 isolates of Pst,including 6 Chinese prevalent races *** analysis indicated that a single dominant gene was responsible for the stripe rust resistance in CI110,temporarily designated YrC110.A molecular map,harboring YrC110 and 9 linked SSR markers,was constructed through simple sequence repeat（SSR）,and bulked segregant *** linked markers and YrC110 were assigned on the short arm of chromosome 1B using the Chinese Spring nullisomic-tetrasomic and ditelosomic *** postulation based on seedling reaction patterns to 30 Pst isolates suggested that the resistance gene YrC110 seemed different from the other known resistance genes tested,such as Yr9,Yr10,Yr15,Yr24,and Yr26/*** SSR markers Xbarc187150,Xgwm18227,Xgwm11223,and Xbarc240292 distinguished YrC110 from Yr10,Yr15,Yr24,and Yr26/YrCH42,and could be used as diagnostic ones for YrC110 in wheat resistant breeding programs against stripe rust.

关键词： synthetic wheat Puccinia striiformis f.sp.tritici resistance gene simple sequence repeat(SSR) marker gene postulation

Molecular Characterization of a Triticum durum-Haynaldia villosa Amphiploid and Its Derivatives for Resistance to Gaeumannomyces graminis var. tritici

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2007年第5期6卷 513-521页

作者： HUANGDa-hui LIN zhi-shan CHEN Xiao ZHANG Zeng-yan CHEN Cai-ceng CHENG Shun-he xin zhi-yong Key Laboratory of Crop Genetics and Breeding of Ministry of Agriculture~Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100094 P.R. China Institute of Agricultural Science of Lixiahe District Yangzhou 225007 P.R.China

Take-all is a serious disease found in wheat across the world. Haynaldia villosa is considered to be resistant to take-all at a high level. TH3 was an amphiploid （2n =42, AABBVV） between Triticum durum and Haynaldia viUosa with significant resistance to take-all fungus isolated from China. In greenhouse experiment, the derivatives of the hybrid between wheat and TH3 showed better resistance to take-all than that of the wheat control. One of the derivatives named HW918-5 was selected for further analysis. Cytological and genomic in situ hybridization （GISH） analysis indicated that a monotelosome originated from H. villosa existed in the genome of the offspring of the line HW918-5. The monotelosome with promising resistant gene for take-all was located on the 3V chromosome of H. villosa in the further PCR-based molecular analysis.

关键词： AFLP Gaeumannomyces graminis var. tritici genomic in situ hybridization Haynaldia villosa Triticum aestivum

国外儿童关系性攻击影响因素及测量方法新进展

在线全文

学校读者我要写书评

暂无评论

医学与哲学（A） 2013年第1期34卷 52-54页

作者：王伟辛志勇山西大同大学医学院心理系山西大同037009 中国人民大学心理学系北京100872 中央财经大学社会发展学院北京100081

关系性攻击是对他人人际关系的攻击。儿童关系性攻击受到很多因素的影响,其中主要包括父母的教养方式、同胞、同伴和媒体等。由于儿童关系性攻击具有隐蔽性的特点,所以儿童关系性攻击的测量方法主要包括社会测量评价、教师评定和自我报... 详细信息

关系性攻击是对他人人际关系的攻击。儿童关系性攻击受到很多因素的影响,其中主要包括父母的教养方式、同胞、同伴和媒体等。由于儿童关系性攻击具有隐蔽性的特点,所以儿童关系性攻击的测量方法主要包括社会测量评价、教师评定和自我报告等,每种方法都具有各自的优点和缺点,多种方法联合使用会更准确。

关键词：关系性攻击父母教养方式测量方法

自钻自攻螺钉与不同基材连接节点抗拉拔承载力试验与理论研究

在线全文

学校读者我要写书评

暂无评论

工程力学 2023年第1期40卷 190-200页

作者：汪大洋辛志勇区彤潘步新叶锡钧张永山广州大学土木工程学院广州510006 珠海安维特工程检测有限公司珠海519100 广东省建筑设计研究院广州510145

螺钉与基材连接节点的力学性能是大跨金属屋面研究的关键问题之一,可靠的连接节点是确保屋面抗风安全的重要组件之一。开展了碳钢螺钉与铝基材(CSA节点)、不锈钢复合螺钉与铝基材(SSA节点)、不锈钢复合螺钉与钢基材(SSS节点)三类节点共... 详细信息

螺钉与基材连接节点的力学性能是大跨金属屋面研究的关键问题之一,可靠的连接节点是确保屋面抗风安全的重要组件之一。开展了碳钢螺钉与铝基材(CSA节点)、不锈钢复合螺钉与铝基材(SSA节点)、不锈钢复合螺钉与钢基材(SSS节点)三类节点共360组样本试验研究,分析了三类节点的损伤失效模式,揭示了抗拉拔承载力的参数影响规律,建立了节点承载力设计计算公式。结果表明:两种钻入方式下三类节点均呈现螺钉与基材共同承载、到二者咬合处产生损伤变形行为、再到螺钉被拔出基材的失效模式,节点抗拉拔能力依次为SSS节点、CSA节点、SSA节点;基材厚度、螺钉直径和螺距均对节点承载力产生影响,尤以基材厚度和螺距最为显著,同等条件下改变螺距对三类节点承载力的影响最高达94.54%;所提三类节点承载力设计计算公式与试验结果吻合度良好。

关键词：自钻自攻螺钉型钢基材铝基材拉拔承载力性能试验

Cloning and Characterization of a Family of Disease Resistance Gene Analogs from 6VS of Haynaldia villosa

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2003年第8期2卷 937-942页

作者： KONG Fan-jing, MA You-zhi, CHEN Xiao and xin zhi-yong(Institute of Crop Breeding and Cultivation , Chinese Academy of Agricultural Sciences , Beijing 100081,P. R. China Open Laboratory of Saline Lake Resources and Environment of Ministryof Land and Resources , Beijing 100037 , P.R. China) Institute of Crop Breeding and Cultivation Chinese Academy of Agricultural Sciences Beijing 100081P.R. China Open Laboratory of Saline Lake Resources and Environment of Ministry of Land and Resources Beijing 100037 P.R. China Institute of Crop Breeding and Cultivation Chinese Academy of Agricultural Sciences Beijing 100081P.R. China

In the present study, microdissection of 6VS and the cloning of the resistance gene analogs(RGA)from them were reported. The 6VS were microdissected with needle and 10 types of resistance gene analogs were obtained by PCR with degenerate oligonucleotide primer designed according to resistance genes. They were designated as Hvrgak1-Hvrgak10, GenBank accession numbers are AF387113-AF387121, AY040671- AY040672. Identity among RGAs was about 10-50%, and identity with cloned R gene from plants was 5-20%. Southern hybridization analysis results showed 3 RGAs, Hvrgak2, Hvrgak4, and Hvr-gak5 were linked with wheat powdery mildew resistance. These RGAs may be used as direct entrance or probes for cloning the disease resistance genes.

关键词： 6VS of Haynaldia Villosa Microdissection Resistance gene analogs(RGA) Cloning

Study on the Factors Influencing the Efficiency of Wheat Transformation

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2002年第1期1卷 30-35页

作者： YE xing-guo, XU Hui-jun, DU Li-pu and xin zhi-yong( Key Laboratory for Crop Genetics and Breeding of Agricultural Ministry, Institute of Crop Breeding and Cultivation , Chinese Academy of Agricultural Sciences, Beijing 100081) Key Laboratory for Crop Genetics and Breeding of Agricultural Ministry Institute of Crop Breeding and Cultivation Chinese Academy of Agricultural Sciences Beijing 100081

Wheat transformation efficiency is closely related to several factors such as receptor genotype, constructed plasmid and selection procedure after bombardment or co-cultivation. In our study, several kinds of antibiotics, which were normally used in plant transformation to the selection genes of nptII, bar and hpt, were tested for the optimal concentrations for wheat transformation. The results showed that 25 - 50mg/L of geneticin (G418) was suitable for the selection of nptll, kanamycin or neomycin was not suitable for use. 3 -5mg/L of phosphinothricin (PPT) or biolaphos could be used for the selection of bar, 100 - 150mg/L of hygromycin for the selection of hpt. Yangmai 158 and Yangmai 10 with high tissue culture response and good agronomic characteristics were screened from 25 potential Chinese wheat cultivars. The concentration changing of selectable agent in selection medium was helpful to obtain enough regeneration plantlets with strong root system.

关键词： Wheat Transformation Selectable marker Receptor genotype Selection procedure

病原诱导的小麦转录因子TaERF1b基因的分离和表达

在线全文

学校读者我要写书评

暂无评论

中国农业科学 2008年第4期41卷 946-953页

作者：董娜张增艳辛志勇中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程/农业部作物遗传育种重点开放实验室中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程/农业部作物遗传育种重点开放实验室北京100081

【目的】研究一个病原诱导的小麦ERF转录因子基因在对纹枯病菌、赤霉病菌防御反应中的作用。【方法】应用生物信息学、RT-PCR、RACE方法,从赤霉病菌诱导的小麦中,分离ERF转录因子基因的全长cDNA序列;利用半定量RT-PCR方法,分析该基因对... 详细信息

【目的】研究一个病原诱导的小麦ERF转录因子基因在对纹枯病菌、赤霉病菌防御反应中的作用。【方法】应用生物信息学、RT-PCR、RACE方法,从赤霉病菌诱导的小麦中,分离ERF转录因子基因的全长cDNA序列;利用半定量RT-PCR方法,分析该基因对小麦纹枯病菌、赤霉病菌及MeJA、ET和SA处理的应答表达情况。【结果】从赤霉病菌诱导的抗赤霉病小麦品种苏麦3号cDNA中,分离出1个编码ERF转录因子基因的全长cDNA序列。该基因暂被命名为TaERF1b,编码由280个氨基酸组成的蛋白质TaERF1b。TaERF1b具有保守的ERF/AP2结构域,但TaERF1b蛋白全长氨基酸序列与已克隆的ERF蛋白同源性较低(<36.5%)。TaERF1b基因表达分析的结果表明,纹枯病菌、赤霉病菌侵染可快速诱导抗病小麦中TaERF1b基因的上调表达,该基因转录表达水平在防卫相关激素乙烯、茉莉酸处理早期显著增强,而且TaERF1b对外源乙烯、茉莉酸处理的响应时期早于对纹枯病菌、赤霉病菌的响应时期。【结论】分离出一个受病原诱导的小麦ERF新基因TaERF1b,其编码蛋白TaERF1b为植物ERF转录因子家族的一个新成员,可能通过茉莉酸、乙烯信号途径介导小麦对纹枯病菌、赤霉病菌的防御反应。

关键词：小麦 ERF转录因子克隆纹枯病菌赤霉病菌表达防御反应

Study on the Obtaining of Transgenic Wheat with GNA Alien Gene by Biolistic Particle

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2002年第1期1卷 25-29页

作者： XU Qiong-fang, LI Lian-cheng, CHEN Xiao, TIAN Fang, MA You-zhi, YE xing-guo, ZHANG Zeng-yan, XU Hui-jun and xin zhi-yong( Key Laboratory of Agricultural Ministry for Crop Genetics and Breeding, Institute of Crop Breeding and Cultivation ,Chinese Academy of Agricultural Sciences, Beijing 100081 ) Key Laboratory of Agricultural Ministry for Crop Genetics and Breeding Institute of Crop Breeding and Cultivation Chinese Academy of Agricultural Sciences Beijing 100081

The immature embryos of wheat plants, cv. Jing 411, 12 - 14 days after pollination, were cultured on SD2 medium for callus induction. After 10 days culture, 800 wheat calli were bombarded by biolistic particle coated with the DNA of plasmid pBI121-2 harboring both Galanthus nivalis agglutinin gene and bar gene. 67 green plants were finally regenerated from the bombardment calli on selection medium containing 4mg/L Basta. The results of bioassay by both inoculating wheat aphids onto the plants and applying Basta solution of 50 mg/L and 75 mg/L onto the wheat leaves in the field, and the molecular analysis, such as PCR and Southern blotting, indicated that 8 T2 plants contaning the target genes were obtained.

关键词： Galanthus nivalis agglutinin bar gene Particle bombardment Transgenic wheat

在线全文

学校读者我要写书评

暂无评论

无机粒子复配聚磷酸铵对硅橡胶性能的影响

华南理工大学学报（自然科学版） 2017年第11期45卷 63-70页

作者：罗远芳丁勇薛锋贾志欣陈勇军贾德民华南理工大学材料科学与工程学院广东广州510640

为研究不同形貌结构的无机粒子复配聚磷酸铵对硅橡胶阻燃性能和机械性能的影响,分别将经偶联剂表面处理后的针状的硅灰石(WS)、中空管状的埃洛石(HNTs)、片层状的磷酸锆(α-ZrP)与聚磷酸铵加入硅橡胶基体中,通过机械共混制备了阻燃硅橡... 详细信息

为研究不同形貌结构的无机粒子复配聚磷酸铵对硅橡胶阻燃性能和机械性能的影响,分别将经偶联剂表面处理后的针状的硅灰石(WS)、中空管状的埃洛石(HNTs)、片层状的磷酸锆(α-ZrP)与聚磷酸铵加入硅橡胶基体中,通过机械共混制备了阻燃硅橡胶复合材料(FRSR).利用SEM、TEM和激光粒度分析仪对3种无机粒子的形貌结构、粒径分布及在FRSR中的分散性进行了表征,采用万能试验机、热失重分析仪、垂直燃烧仪、极限氧指数仪等对FRSR的机械性能、热稳定性和阻燃性能进行了研究.结果表明:3种无机粒子在FRSR中均能保持初始形貌,在基体中能较好地分散;当无机粒子与聚磷酸铵复配物的添加量为25 phr时,填充1 phr改性HNTs的FRSR拥有更高的机械性能(拉伸强度为8.8 MPa,断裂伸长率为218.6%),填充1 phr改性α-ZrP的FRSR的阻燃性能和热稳定性最好(极限氧指数为32.3,垂直燃烧级别为V-0,起始分解温度为312.9℃).文中还采用SEM对FRSR燃烧后的碳渣进行了分析,并对残渣形成机制进行了初步探讨.

关键词：硅橡胶无机粒子聚磷酸铵阻燃性能机械性能