检索结果-南通市图书馆

Microplate chemiluminescence enzyme immunoassay for the quantitative evaluation of carbohydrate antigen 72-4 in human serum

引用

Chinese Science Bulletin 2008年第19期53卷 2958-2963页

作者： JIN Hui WANG Xu xin tianbing GAO Peng LIN Jin-Ming LIANG ShuXuan College of Chemistry and Environmental Science Hebei University Baoding 071002 China Department of Chemistry Tsinghua University Beijing 100084 China Beijing Chemclin Biotech Co. Ltd. Beijing 100094 China

A highly sensitive and specific microplate chemiluminescence enzyme immunoassay (CLEIA) was developed for the quantitative evaluation of carbohydrate antigen 72-4 (CA72-4) in human serum, using luminol-H2O2 catalyzed by horseradish peroxidase (HRP) as the chemiluminescence system. The simple and quick determination was accomplished through a sandwich reaction mode. Several physico-chemical parameters of the immunoreaction, including incubation conditions, antibody coating conditions, dilution ratio of anti-CA72-4-HRP conjugate, and chemiluminescence reaction time, were studied and optimized. The proposed method exhibited a linear range of 0―200 U/mL with correlation coefficient and detection limit of 0.9995 and 0.18 U/mL, respectively. The inter-assay and intra-assay coeffi-cients of variation (CV) were both less than 10%. The average recovery of two clinical sera with low and high concentration CA72-4 was 99.3% and 98.7%, respectively. Normal tumor markers, including AFP, CEA, CA24-2, CA19-9 and CA15-3, did not cross-react with each other. The method's stability was evaluated by assessing its analytical performance after storing the immunoreagents at 4℃ and 37℃ for 7 days. Little difference was found, indicating satisfactory stability of the method. The present method has been successfully applied to the detection of CA72-4 human serum, and showed a good correlation with the commercially available ELISA kit (r 2=0.9383). This method showed great potential in the fabrication of diagnostic kit for CA72-4, and could be well used in diagnosis of cancer in clinical practice.

关键词：化学发光酶学免疫检测肿瘤标记

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Oxidative thiocyanation of allylic alcohols:an easy access to allylic thiocyanates with K_(2)S_(2)O_(8) and NH_(4)SCN

引用

Organic Chemistry Frontiers 2022年第4期9卷 966-972页

作者： Keyuan Zhang tianbing Liang Yulong Wang Chonglong He Mingyou Hu xin-Hua Duan Le Liu School of Chemistry Xi’an Key Laboratory of Sustainable Energy Materials ChemistryMOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed MatterXi’an Jiaotong UniversityXi’an 710049China

A practical method for the synthesis of allylic thioacyanates from allylic alcohols was disclosed employing K_(2)S_(2)O_(8) as the oxidant and NH_(4)SCN as the thiocyanate *** introducing a leaving group in advance,a series of disubstituted and monosubstituted allylic thiocyanate compounds were obtained in moderate to good *** transition metal-free method features mild reaction conditions,broad sub-strate scope and operational *** potential application of this method was demonstrated by some derivatization reactions of the product and mechanistic investigation indicated that a radical process is involved.

关键词： alcohols simplicity substituted

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Primary reconstruction of Digital nerve defect by human acellular nerve graft

Primary reconstruction of Digital nerve defect by human acel...

引用

2015年浙江省显微外科学会年会暨浙江省手外科学术年会

作者： Xueyuan Li Haoliang Hu Janrong Fei Wang xin tianbing Wang Peixun Zhang 宁波市第六医院手外科 Traumatic & Orthopedic department of Peking university People's University Hospital

PURPOSE:To explore the clinical feasibility and effectiveness of human acellular nerve graft for reconstruction of digital nerve defects within 5 ***:15 patients with 18 digital nerve injury with defect in the *** wound were evaluated cautiously for primary nerve allograft *** clean and mild contaminated wound were *** average nerve defectswasl8 mm(5-50 mm).All the patients underwent primary nerve repair with human acellular nerve graft and followed up 6 months to 2 *** sensory were evaluated by monofilaments and 2-point *** immune rejections such as wound delay were also *** of the patients received immunosuppressive therapy ***:According to Mackinnon-Dellon scale for stratification of 2PD criteria,9 of 18(50%) digits had excellent results,7 of 18(38.9%) had good results,2 of 18(11.1%) digits had poor ***-Weinstein monofilament testing results normal in 7 digits,good in 7 digits,fair in 2 digits and poor in 2 *** were no signs of infection and extrusion except in 1 case showed redness and swelling 3 days after surgery,which was controlled by prolonged antibiotic ***:Human acellular nervegraft is proved to be a safe and effective alternative for the reconstruction of peripheral digital nerve deficits measuring up to 50 mm,which avoid the donor site disfunction.

关键词： Allograft digital nerve nerve conduit nerve reconstruction peripheral nerve

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

好氧堆肥微生物选育及应用研究进展

引用

工业微生物 2022年第4期52卷 55-62页

作者：朱鑫郜天兵杨太婷河南热联科技有限公司河南郑州450000

堆肥是多种微生物群落共同作用的动态过程,堆肥的进程、最终产品质量与堆肥内微生物种类和活性密切相关。本文概述了好氧堆肥内微生物的影响因素,近年来国内外报道的好氧堆肥中常见的优势微生物与常用的堆肥微生物选育方法,以及将选育... 详细信息

堆肥是多种微生物群落共同作用的动态过程,堆肥的进程、最终产品质量与堆肥内微生物种类和活性密切相关。本文概述了好氧堆肥内微生物的影响因素,近年来国内外报道的好氧堆肥中常见的优势微生物与常用的堆肥微生物选育方法,以及将选育出的微生物制成菌剂应用于堆肥有关研究。本文为好氧堆肥微生物的选育与应用提供了一定的参考。

关键词：好氧堆肥微生物菌剂菌种选育进展

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

床旁超声指导肺部机械辅助排痰治疗重症肺炎患儿的疗效观察

引用

当代医学 2022年第25期28卷 175-178页

作者：杨弋仙陈天兵彭新江西省儿童医院急诊科江西南昌330038

目的探讨床旁超声指导肺部机械辅助排痰治疗重症肺炎患儿的临床疗效。方法选取2019年1月至2021年1月入住本院儿童重症监护室(PICU)和/或呼吸科的100例重症肺炎患儿作为研究对象,按照随机数字表法分为两组,各50例。观察组采用床旁超声指... 详细信息

目的探讨床旁超声指导肺部机械辅助排痰治疗重症肺炎患儿的临床疗效。方法选取2019年1月至2021年1月入住本院儿童重症监护室(PICU)和/或呼吸科的100例重症肺炎患儿作为研究对象,按照随机数字表法分为两组,各50例。观察组采用床旁超声指导肺部机械辅助排痰治疗,对照组行人工叩背排痰治疗,比较两组治疗24、48、72 h后的心率、呼吸频率、动脉血二氧化碳分压(PCO_(2))、氧合指数及吸氧时间、入住PICU时间、总住院时间。结果治疗24、48、72 h后,观察组心率、呼吸频率均低于对照组,差异有统计学意义(P<0.05);治疗24、48、72 h后,观察组PCO_(2)均低于对照组,氧合指数均高于对照组,差异有统计学意义(P<0.05);观察组吸氧时间、入住PICU时间、总住院时间均短于对照组,差异有统计学意义(P<0.05)。结论床旁超声指导下肺部机械辅助排痰治疗重症肺炎患儿能有效维持患儿生命体征稳定,改善患儿血气指标,缩短吸氧时间及住院时间,促进患儿康复,值得临床推广应用。

关键词：床旁超声肺部机械辅助排痰重症肺炎

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

精索静脉曲张对青春期大鼠睾丸生精细胞HIF-1α和Bax表达的影响

引用

内分泌外科杂志 2007年第2期1卷 86-88页

作者：吕天兵苟欣余周吴跃重庆医科大学附属第一医院泌尿外科重庆400016

目的：研究精索静脉曲张（VC）对大鼠睾丸生精细胞低氧诱导因子-1α（hypoxia—inducible factor-lα、HIF-1α）和Bax表达的影响，探索VC引起不育的机理。方法：采用青春期雄性Wistar大鼠复制左侧VC模型，将25只大鼠随机分为VC组（n=1... 详细信息

目的：研究精索静脉曲张（VC）对大鼠睾丸生精细胞低氧诱导因子-1α（hypoxia—inducible factor-lα、HIF-1α）和Bax表达的影响，探索VC引起不育的机理。方法：采用青春期雄性Wistar大鼠复制左侧VC模型，将25只大鼠随机分为VC组（n=15）和假手术组（SOG）（n=10），于术后30天一并处死、取左侧睾丸，用免疫组化SP法检测HIF-1α和Bax的表达。结果：HIF-1α和Bax在VC组左侧睾丸组织中的表达均明显高于假手术组（SOG）。结论：实验性VC可以改变青春期大鼠睾丸组织生精细胞HIF-1α和Bax的表达。

关键词：精索静脉曲张生精细胞凋亡 HIF—1α Bax

来源：

维普期刊数据库

同方期刊数据库评论