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An Arabidopsis Class II Formin,AtFH19,Nucleates Actin Assembly,Binds to the Barbed End of Actin Filaments,and Antagonizes the Effect of AtFH1 on Actin Dynamics

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Journal of Integrative Plant Biology 2012年第10期54卷 800-813页

作者： Yiyan Zheng haibo xin Jinxing Lin Chun-Ming Liu Shanjin Huang Key Laboratory of Plant Molecular Physiology Institute of Botanythe Chinese Academy of SciencesBeijing 1%0093China University of Chinese Academy of Sciences Beijing 10049China Ornamental Horticulture and Landscape Architecture China Agricultural UniversityBeijing 100094China

Formin is a major protein responsible for regulating the nucleation of actin filaments, and as such, it permits the cell to control where and when to assemble actin arrays. It is encoded by a multigene family comprising 21 members in Arabidopsis thaliana. The Arabidopsis formins can be separated into two phylogenetically-distinct classes： there are 11 class I formins and 10 class II formins. Significant questions remain unanswered regarding the molecular mechanism of actin nucleation and elongation stimulated by each formin isovariant, and how the different isovariants coordinate to regulate actin dynamics in cells. Here, we characterize a class II formin, AtFH19, biochemically. We found that AtFH19 retains all general properties of the formin family, including nucleation and barbed end capping activity. It can also generate actin filaments from a pool of actin monomers bound to profilin. However, both the nucleation and barbed end capping activities of AtFH19 are less efficient compared to those of another well-characterized formin, AtFHI. Interestingly, AtFH19 FH1FH2 competes with AtFH1 FHIFH2 in binding actin filament barbed ends, and inhibits the effect of AtFH1 FHIFH2 on actin. We thus propose a mechanism in which two quantitatively different formins coordinate to regulate actin dynamics by competing for actin filament barbed ends.

关键词： Actin actin-binding proteins actin nucleation Arabidopsis cytoskeleton formin.

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Design and testing of micro fluidic chemical analysis chip integrated with micro valveless pump

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Science China(Technological Sciences) 2005年第3期48卷 326-337页

作者： FU xin XIE haibo YANG Huayong JIA Zhijian FANG Qun State Key Laboratory of Fluid Power Transmission and Control Zhejiang UniversityHangzhou 310027China State Key Laboratory of Fluid Power Transmission and ControlZhejiang UniversityHangzhou 310027China State Key Laboratory of Fluid Power Transmission and ControlZhejiang UniversityHangzhou 310027China Institute of Microanalytical SystemsZhejiang UniversityHangzhou 310028China Institute of Microanalytical SystemsZhejiang UniversityHangzhou 310028China

A new structure and working principle of the chip integrated with micro valveless pump for capillary electrophoresis was proposed in this paper. The micro valveless pump with plane structure has advantages of simple structure, and the process technology is compatible with existing micro chips for capillary electrophoresis. Based upon the mathematical model, simulation study of micro pump was carried out to investigate the influence of structural parameters on flow characteristics, and the performance of the integrated micro pump was also tested with different control parameters. The simulation results agree with the experimental results. Three samples, which are amino acid, fluorescein and buffer solution, have been examined with this chip. The results of the primary experiments showed that the micro valveless pump was promising in the integration and automatization of miniature integrated fluidic systems.

关键词： MEMS,micro valveless pump,micro fluidic chip,CFD,capillary electrophoresis.

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Chromosome-scale genome assembly of marigold (Tagetes erecta L.):An ornamental plant and feedstock for industrial lutein production

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Horticultural Plant Journal 2023年第6期9卷 1119-1130页

作者： haibo xin Fangfang Ji Jie Wu Shiya Zhang Caijie Yi Shiwei Zhao Richen Cong Liangjun Zhao Hua Zhang Zhao Zhang Beijing Key Laboratory of Greening Plants Breeding Beijing Academy of Forestry and Landscape ArchitectureBeijing 100102China Beijing Key Laboratory of Development and Quality Control of Ornamental Crops Department of Ornamental HorticultureChina Agricultural UniversityBeijing 100193China

Marigold(Tagetes erecta L., African marigold) is a widely grown ornamental plant and a main source of the carotenoid lutein for the industrial production of pharmaceuticals, food coloring, and feed additives. To gain a deeper understanding of the genetic mechanism of lutein in marigold, a chromosome-scale assembly of the marigold(T. erecta V-01) genome was completed based on Illumina, PacBio, and Hi-C reads. The707.21-Mb assembled genome consisted of 35 834 annotated protein-coding genes, with 97.7% genomic integrity. We anchored 87.8% of the contigs(covering 621.20 Mb) to 12 pseudochromosomes, bringing the scaffold N50 length to 54.15 Mb. Phylogenetic analysis showed that marigold was closely related to the Asteraceae species bitter vine(Mikania micrantha) and sunflower(Helianthus annuus), all three of which originated in the Americas. Marigold diverged from the sunflower clade 23.57 million years ago(MYA) and from M. micrantha 19.59 *** has undergone three whole-genome duplication events, as well as a recent whole-genome duplication event(WGD-2) common to *** and M. micrantha. Marigold gene families were significantly less expanded than those of M. micrantha or H. annuus, and the marigold genome contained significantly fewer interspersed repeats, which might account for its smaller genome. In addition, a range of candidate genes involved in the lutein biosynthetic pathway were identified. The high-quality reference genome obtained in this study provided a valuable genomic resource for studying the evolution of the Asteraceae family and for improving marigold breeding strategies.

关键词： T.erecta L. Genome Gene family Carotenoid Lutein

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Bimagnetic metal constructed core-shell structure of Fe@Co nanowires for multi-band microwave absorption

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Progress in Natural Science:Materials International 2024年第2期34卷 354-361页

作者： Ping-an Yang Rui Cai haibo Ruan Nanqing Zhang xin Huang Rui Li Yuxin Zhang Yi Lu Zhihao Zhou School of Automation Chongqing University of Posts and Telecommunications Chongqing Key Laboratory of Materials Surface & Interface Science Chongqing University of Arts and Sciences College of Engineering and Technology Zunyi Normal College College of Material Science and Engineering Chongqing University

Controlled fabrication of materials by surface modification techniques has been a hot research topic. In this paper,one-dimensional(1D) core-shell structure Fe@Co nanowires(NWs) were successfully prepared by anchoring 0-dimensional(0D) Co nanoparticles on 1D Fe NWs based on the in situ reduction method. The electromagnetic parameters of the specimens at a filler mass fraction of 25 wt% were tested and the microwave loss mechanism was deeply analyzed. Binary magnetic metals in a core-shell structure have excellent multi-band microwave absorption capability(S-band, X-band and Ku-band). This is due to the heterogeneous interface and magnetic coupling effects tuning the dielectric and magnetic loss. This study offers a workable plan for creating effective multi-band magnetic metal-based microwave absorbers.

关键词： Microwave absorption One-dimensional nanowires Core-shell structure Magnetic coupling Interface

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Lodging-Resistance Breeding of Platycodon grandiflorus Using Distant Hybridization

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American Journal of Plant Sciences 2015年第18期6卷 2844-2849页

作者： Yan Ma Yan Lin Huali Zhang Fang Liang Liping Sun Maoliang Wang haibo xin Beijing Institute of Landscape and Architecture Beijing Key Laboratory of Green Plants Breeding Beijing China

The distant hybridization was used in lodging-resistance breeding of Platycodon grandiflorus. The parents were Platycodon grandiflorus (♀) and Campanula medium (♂). 187 seeds of F1 were harvested by using the inter-generic hybridization in 2009 and 2010, 2 repeat, and 88 normal plants were obtained. The characteristics of leaves, stems and branches were the same as female for F1 generation, F2 generation, backcross generation and S1 generation, and there were some different characteristics with female which were flower colors, branching habit and plant height. A lodging-resistance plant was selected in F1, which was about 75 cm high, deep pink flower color and developed lateral branches. The DNA groups of each generation were separated by 1% agarose gel electrophoresis and there were not significant differences. Among the each generation many flower colors and forms were obtained.

关键词： Lodging-Resistance Breeding Platycodon grandiflorus Campanula medium Distant Hybridization

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Gene Cloning and Expression of the Pyrroline-5-carboxylate Reductase Gene of Perennial Ryegrass(Lolium perenne)

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Horticultural Plant Journal 2015年第2期1卷 113-120页

作者： CAO Li WEI Shuqiang HAN Lei QIAN Yongqiang ZHANG Huali xin haibo SUN Zhenyuan Department of Horticulture and Landscape Yanbian University Key Laboratory of Tree Breeding and Cultivation State Forestry Administration/Research Institute of Forestry Chinese Academy of Forestry Beijing Key Laboratory of Greening Plants Breeding Beijing Institute of Landscape and Architecture

Salt and drought limit the range of applications of perennial ryegrass(Lolium perenne L.), which is one of the important turf and forage grasses. Previous studies have suggested that pyrroline-5-carboxylate reductase(P5CR) might play a central role in proline accumulation in plants that are responsive to stresses. In the present study, the Lolium perenne L. pyrroline-5-carboxylate reductase(LpP 5CR) gene was cloned from leaves of the cultivar ‘Derby' using the RACE technique. The full-length LpP 5CR gene was 1 047 bp in length, which comprised an open reading frame(ORF) of 840 bp in size. Sequence alignment revealed that the putative Lp P5 CR had a 94.3% similarity to Ta P5 CR. q RT-PCR displayed that the mR NA levels of the LpP 5CR gene were almost the same as that in the roots, stems, and leaves of perennial ryegrass seedlings subjected to normal condition or NaC l treatment for 1 h. Moreover, the transcription level of LpP 5CR was up- or down- regulated with Na Cl, polyethylene glycol(PEG), cold, or abscisic acid(ABA) treatment for 3 to 48 h. In addition, confocal microscopy localized the GFP-Lp P5 CR fusion protein to the cytoplasm of onion epidermal cells. These findings suggest that LpP 5CR encodes a cytoplasmic P5 CR protein that plays an important role in the response of perennial ryegrass to various stresses.

关键词： Lolium perenne pyrroline-5-carboxylate reductase proline subcellular localization gene expression

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Study on an intelligent fault-tolerant technique for multiple satellite configured navigation under highly dynamic conditions

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Science China(Information Sciences) 2011年第3期54卷 529-541页

作者： WANG ShiCheng 1 , ZHAO xin 1 , MIN haibo 1 , LIU ZhiGuo 1 & SI XiaoSheng 2 1 High-Tech Institute of Xi’an, Xi’an 710025, China 2 Department of Automation, Tsinghua University, Beijing 100084, China High-Tech Institute of Xi’an Xi’an China Department of Automation Tsinghua University Beijing China

The probability of pseudo-range information being faulty will increase when there is increased noise in the receiver due to highly dynamic conditions. In this paper, an intelligent fault-tolerant federated Kalman filter (IFFKF) is presented to solve the problem of invalid position information as a result of the uncertainty in the category and quantity of failed satellites in a multiple configured satellite system. At the same time, a corresponding intelligent fault-tolerant system structure based on a cell-level fusion mode of operation was designed. Furthermore, a fault diagnosis strategy is proposed to deal with gradually or suddenly changing faults. By monitoring the residual and the residual rate, an observation quality gene is obtained using a fuzzy logic control system, and the local filter is adaptively adjusted on-line. Consequently, any filtering divergence arising from a gradually changing fault can, to a large extent, be prevented. The results show that the proposed algorithm and structure can achieve reliable positioning by fully utilizing new multiple satellites, monitoring and managing the gradual fault, as well as detecting and isolating any sudden fault in a synchronous manner. As such, the proposed algorithm has the potential to improve the reliability of a multiple satellite configured navigation system.

关键词： multiple satellite configured systems fuzzy logic control intelligent fault-tolerant filtering federated filtering fault detection and isolation

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焊接机器人在机车构架生产中的应用

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金属加工（热加工） 2008年第24期 53-55页

作者：辛海波上海交通大学 200240

针对焊接机器人的特点,分析焊接机器人在GE机车转向架构架生产中的应用,介绍了焊接机器人的工艺要点,在装配和焊接过程中的技术要求,工装设计以及编程等问题。焊接机器人大大提高了焊接质量和效率。

关键词：焊接机器人转向架构架应用生产机车工艺要点焊接过程工装设计

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Multiphysics simulation and optimization of the in-situ beneficiation process of ilmenite minerals from lunar regolith

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Science China Earth Sciences 2024年

作者： Peng ZHANG xin LIU Guanghui LIU Wei DAI Hanzhe YANG haibo ZHENG Zhi WANG Ran NIU Yifan BAI Yang ZHANG Chengbao LIU Ge YANG Junwei YANG Guang ZHANG Technology and Engineering Center for Space Utilization Chinese Academy of Sciences Beijing Hegong Simulation Co. Ltd

The exploration and utilization of lunar resources have become the major objectives of future lunar exploration missions. The classified collection and processing of lunar minerals is the basic prerequisite for the in-situ exploration and utilization of lunar resources. In this study, we propose a scheme for in-situ separation of magnetic minerals from lunar soils using the dry magnetic separation method. The scheme can realize the purpose of separating magnetic minerals from lunar regolith by altering the motion trajectory of lunar regolith particles with different magnetic properties. Detailed modeling of the dry magnetic separation device is conducted, and the three-dimensional distribution of magnetic parameters is calculated and analyzed. Based on the characterization and analysis of the ChangE-5’s samples, the morphologies and physical parameters of lunar regolith particles are modeled and simulated. A discrete element simulation of the motion process of lunar regolith particles under multi-physical field conditions is performed, and the influences of key parameters on the beneficiation performance of lunar magnetic minerals are investigated. Our results indicate that optimal separation and beneficiation of lunar regolith particles can be achieved under specific conditions of magnetic field strength and drum speed. Additionally, we analyze the effects of lunar weak gravity conditions and the charged nature of lunar particles on the beneficiation process, showing that these factors can obviously enhance the beneficiation process of lunar ilmenites. This study demonstrates the feasibility of using the dry magnetic separation method for the beneficiation of magnetic minerals from lunar regolith, providing a theoretical basis for the scientific tasks of collecting, processing, and utilizing lunar mineral resources.

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野生翠雀传粉生物学特性研究

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西北植物学报 2014年第9期34卷 1789-1794页

作者：张华丽赵剑颖辛海波张金凤北京市园林科学研究院北京100102 绿化植物育种北京市重点实验室北京100102 泛华集团设计八所北京100082 北京林业大学北京100083

对从北京郊区引种的野生翠雀（Delphiniun grandi florum L.）的开花习性进行了观察,对其花粉活力、柱头可授性,花粉/胚珠比（P/O）,杂交指数（OCI）进行了检测,并进行了人工授粉实验.结果表明：（1）翠雀的花序为总状花序,具3~1 5朵花... 详细信息

对从北京郊区引种的野生翠雀（Delphiniun grandi florum L.）的开花习性进行了观察,对其花粉活力、柱头可授性,花粉/胚珠比（P/O）,杂交指数（OCI）进行了检测,并进行了人工授粉实验.结果表明：（1）翠雀的花序为总状花序,具3~1 5朵花,花梗长1.5~3.8 cm,单花期10 d,花序花期15~18 d.（2）翠雀花粉活力日变化呈先升高后降低的趋势;柱头在开花当天至开花后4d无可授性,在开花后的5d、6d有可授性.（3）翠雀的花粉胚珠比（P/O）为2 159.14,杂交指数OCI为5;人工授粉实验表明,翠雀在自然条件下能够结实,结实率为100％;存在自主自花授粉现象,部分自交亲和,结实受传粉者限制;不存在无融合生殖现象.（4）翠雀主要的访花昆虫有四条无垫蜂和短腹管蚜蝇.研究认为,翠雀的繁育系统为兼性异交或专性异交,部分自交亲和,需要传粉者.

关键词：翠雀传粉生物学繁育系统

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