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Effects of Glucocorticoid?Induced Transcript 1 Gene Deficiency on Glucocorticoid Activation in Asthmatic Mice

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Chinese Medical Journal 2018年第23期131卷 2817-2826页

作者： Cheng-Ping Hu qiu-Fen Xun Xiao-Zhao Li xin-Yue Hu Ling Qin Ruo-Xi He Jun-Tao Feng Department of Respiratory and Critical Care Medicine Key Cite of National Clinical Research Center for Respiratory DiseaseXiangya HospitalCentral South UniversityChangshaHunan 410008China Department of Respiratory Medicine The Second Affiliated Hospital of Nanchang UniversityNanchangJiangxi 330006China Department of Nephrology Xiangya HospitalCentral South UniversityChangshaHunan 410008China

Background: Glucocorticoid (GC) is the first?line therapy for asthma, but some asthmatics are insensitive to it. Glucocorticoid?induced transcript 1 gene (GLCCI1) is reported to be associated with GCs efficiency in asthmatics, while its exact mechanism remains unknown. Methods: A total of 30 asthmatic patients received fluticasone propionate for 12 weeks. Forced expiratory volume in 1 s (FEV1) and GLCCI1 expression were detected. Asthma model was constructed in wild?type and GLCCI1 knockout (GLCCI1?/?) mice. Glucocorticoid receptor (GR) and mitogen?activated protein kinase phosphatase 1 (MKP?1) expression were detected by polymerase chain reaction and Western blotting (WB). The phosphorylation of p38 mitogen?activated protein kinase (MAPK) was also detected by WB. Results: In asthmatic patients, the change of FEV1 was well positively correlated with change of GLCCI1 expression (r = 0.430, P = 0.022). In animal experiment, GR and MKP?1 mRNA levels were significantly decreased in asthmatic mice than in control mice (wild?type: GR: 0.769 vs. 1.000, P = 0.022; MKP?1: 0.493 vs. 1.000, P < 0.001. GLCCI1?/?: GR: 0.629 vs. 1.645, P < 0.001; MKP?1: 0.377 vs. 2.146, P < 0.001). Hydroprednisone treatment significantly increased GR and MKP?1 mRNA expression levels than in asthmatic groups; however, GLCCI1?/?.asthmatic mice had less improvement (wild?type: GR: 1.517 vs. 0.769, P = 0.023; MKP?1: 1.036 vs. 0.493, P = 0.003. GLCCI1?/?: GR: 0.846 vs. 0.629, P = 0.116; MKP?1: 0.475 vs. 0.377, P = 0.388). GLCCI1?/? asthmatic mice had more obvious phosphorylation of p38 MAPK than wild?type asthmatic mice (9.060 vs. 3.484, P < 0.001). It was still higher even though after hydroprednisone treatment (6.440 vs. 2.630, P < 0.001). Conclusions: GLCCI1 deficiency in asthmatic mice inhibits the activation of GR and MKP?1 and leads to more obvious phosphorylation of p38 MAPK, leading to a decremental sensitivity to GCs.

关键词： Asthma Glucocorticoid Receptor Glucocorticoid-Induced Transcript 1 Glucocorticoids Mitogen-Activated Protein Kinase Phosphatase-1

Proteomic Portrait of Human Lymphoma Reveals Protein Molecular Fingerprint of Disease Specific Subtypes and Progression

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Phenomics 2023年第2期3卷 148-166页

作者： xin Ku Jinghan Wang Haikuo Li Chen Meng fang Yu Wenjuan Yu Zhongqi Li Ziqi Zhou Can Zhang Ying Hua Wei Yan Jie Jin Shanghai Center for Systems Biomedicine Key Laboratory of Systems Biomedicine(Ministry of Education)Shanghai Jiao Tong UniversityShanghai 200240China Department of Hematology The First Affiliated Hospital of Medical School of Zhejiang UniversityHangzhou 310003China Bavarian Center for Biomolecular Mass Spectrometry Technical University of Munich85354 FreisingGermany Cancer Center Zhejiang UniversityHangzhou 310003China Department of Pathology The First Affiliated Hospital of Zhejiang UniversityHangzhou 310003China Department of Surgical Oncology College of MedicineThe First Affiliated HospitalZhejiang UniversityHangzhou 310003China Key Laboratory of Hematologic Malignancies Diagnosis and TreatmentHangzhou 310003China Present Address:Division of Biology&Biomedical Sciences Washington University in St.Louis School of MedicineSt.Louis 63130USA

An altered proteome in lymph nodes often suggests abnormal signaling pathways that may be associated with diverse lymphatic *** clinical biomarkers for histological classification of lymphomas have encountered many discrepancies,particularly for borderline ***,we launched a comprehensive proteomic study aimed to establish a proteomic landscape of patients with various lymphatic disorders and identify proteomic variations associated with different disease *** this study,109 fresh-frozen lymph node tissues from patients with various lymphatic disorders(with a focus on Non-Hodgkin’s Lymphoma)were analyzed by data-independent acquisition mass spectrometry.A quantitative proteomic landscape was comprehensively characterized,leading to the identification of featured protein profiles for each *** correlations between clinical outcomes and expression profiles of signature proteins were also *** representative signature proteins,phospholipid-binding proteins Annexin A6(ANXA6)and Phospholipase C Gamma 2(PLCG2),were successfully validated via *** also evaluated the capability of acquired proteomic signatures to segregate multiple lymphatic abnormalities and identified several core signature proteins,such as Sialic Acid Binding Ig Like Lectin 1(SIGLEC1)and GTPase of immunity-associated protein 5(GIMAP5).In summary,the established lympho-specific data resource provides a comprehensive map of protein expression in lymph nodes during multiple disease states,thus extending the existing human tissue proteome *** findings will be of great value in exploring protein expression and regulation underlying lymphatic malignancies,while also providing novel protein candidates to classify various lymphomas for more precise medical practice.

关键词： Lymphoma Non-Hodgkin’s Lymphoma Proteomics Stratification Molecular profiling

极端降雨对黄土沟壑区隧洞施工力学效应影响分析

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科学技术与工程 2024年第15期24卷 6388-6397页

作者：尚凯陶虎方自刚樊娜娜万宇兰州交通大学土木工程学院兰州730070

黄土地区输水隧洞的建设中,降雨入渗极大影响着输水隧洞围岩稳定性。利用非饱和渗流理论和数值模拟方法研究了极端降雨条件入渗与黄土沟壑区隧洞的开挖扰动耦合影响下围岩的变形和渗流场演变规律,通过Plaxis3D模型对降雨入渗下新奥法施... 详细信息

黄土地区输水隧洞的建设中,降雨入渗极大影响着输水隧洞围岩稳定性。利用非饱和渗流理论和数值模拟方法研究了极端降雨条件入渗与黄土沟壑区隧洞的开挖扰动耦合影响下围岩的变形和渗流场演变规律,通过Plaxis3D模型对降雨入渗下新奥法施工过程中围岩力学特性、初期支护过程进行流固耦合分析。结果显示,降雨等级越高,围岩孔隙水压力变化幅度越大,对围岩的稳定性越不利;降雨入渗与开挖扰动会加速土层中渗流场和应力场的重分布过程;基于沟道的汇水特性,隧洞围岩含水率在最初5~10 d呈台阶式增长,拱顶、拱腰和拱趾处的最大变形为17.8、28.2、24.6 mm,最大剪切应力为150.6、182.5、175.3 kPa,降雨结束约15~20 d围岩变形速率逐渐减小收敛至特定值,但围岩的应力持续增加,为避免围岩发生塑性变形,故可在15~20 d进行二次衬砌支护。本次研究结果可对极端降雨期黄土沟壑地区隧洞施工安全控制提供参考。

关键词：极端降雨黄土沟壑区隧洞开挖力学响应时效性

在群论框架下电子三重态与声子耦合的理论研究

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物理学报 2011年第1期60卷 552-559页

作者：冯胜奇方海邱庆春韩山师范学院物理与电子工程系潮州521041 汕头大学医学院物理教研室汕头515041

本文基于绝热近似和群论导出了电声耦合系统的哈密顿量的一般形式,讨论了电声耦合系统中的电子算符和活跃的声子模式.应用幺正平移变换和能量最小化方法,进一步计算了正四面体群下T(e+t2)杨-泰勒系统中的激发态能量,从对称性的角度分... 详细信息

本文基于绝热近似和群论导出了电声耦合系统的哈密顿量的一般形式,讨论了电声耦合系统中的电子算符和活跃的声子模式.应用幺正平移变换和能量最小化方法,进一步计算了正四面体群下T(e+t2)杨-泰勒系统中的激发态能量,从对称性的角度分析了T1电子态的能级分裂以及晶格体系的对称性破缺,得出了对称性的破缺方式和电声耦合系统密切相关的结论.结果表明:通过群论与对称性分析完全可以定性地解释由于电声耦合所造成的简并电子态的能级分裂,在某些情况下还可以给出分裂后的基态与激发态的对称性.

关键词：电声耦合杨-泰勒畸变活跃的杨-泰勒声子模式电声耦合哈密顿量

Anion-Responsive Manganese Porphyrin Facilitates Chloride Transport and Induces Immunogenic Cell Death

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CCS Chemistry 2022年第7期4卷 2409-2419页

作者： fang-xin Wang Jie-Wei Liu Xiao-Qiao Hong Cai-Ping Tan Li Zhang Wen-Hua Chen Peter J.Sadler Zong-Wan Mao MOE Key Laboratory of Bioinorganic and Synthetic Chemistry Department of ChemistrySun Yat-Sen UniversityGuangzhou 510275 Department of Chemistry University of WarwickCoventry CV47AL School of Biotechnology and Health Sciences Wuyi UniversityJiangmen 529020

Chloride is the most abundant anion in living *** natural or synthetic chloride anionophores function via hydrogen-bonding ***,dynamic metal-anion coordination can also be an efficient way of transporting chloride across ***,we investigate anion transport by manganese(Ⅲ)meso-tetraphenylporphyrin chloride{[Mn(TPP)Cl],TPP=meso-tetraphenylporphyrin}complex that exhibits labile axial coordination.[Mn(TPP)Cl]showed high chloride transport activity in a bilayer vesicle model with an EC_(50) value of 4.42×10^(−3) mol%.In living cells,[Mn(TPP)Cl]induced rapid chloride influx and *** release of Ca^(2+)and adenosine 5′-triphosphate(ATP),as well as the relocation of calreticulin,revealed that[Mn(TPP)Cl]caused immunogenic cell *** analysis indicated that[Mn(TPP)Cl]impaired several physiological processes,including DNA synthesis,transcription,mitochondrial respiration,RNA translation,and immune *** study suggests that dynamic metal-anion interactions across membranes might provide a practical strategy for the interference of chloride homeostasis.

关键词： manganese porphyrin anionophore chloride transport autophagy immunogenic cell death

头针不同留针时间对脑卒中后运动功能障碍和血清C反应蛋白、血糖、血脂的影响

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广州中医药大学学报 2024年第6期41卷 1510-1516页

作者：钟琪蔡海燕金炳旭霍湛新罗慧艺钟秋霞郭浩讯陈嘉雯邹舒惠广州市番禺区中医院康复科广东广州511401

【目的】观察头针不同留针时间对脑卒中患者运动功能障碍和血清C反应蛋白(CRP)、血糖、血脂的改善情况。【方法】将120例脑卒中后运动功能障碍患者随机分为对照组、观察1组、观察2组,每组各40例。3组患者均采取头针、体针及常规运动康... 详细信息

【目的】观察头针不同留针时间对脑卒中患者运动功能障碍和血清C反应蛋白(CRP)、血糖、血脂的改善情况。【方法】将120例脑卒中后运动功能障碍患者随机分为对照组、观察1组、观察2组,每组各40例。3组患者均采取头针、体针及常规运动康复训练治疗,对照组头针留针30 min,观察1组头针留针1 h,观察2组头针留针2 h,每日1次,每周6次,连续治疗2周。比较3组患者的美国国立卫生研究院卒中量表(NIHSS)、Fugl-Meyer运动评定量表(FMA)、Berg平衡量表(BBS)、改良Barthel指数(MBI)评分以及外周血CRP、空腹血糖(FPG)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)的改善程度,并评价3组治疗方案的安全性。【结果】(1)量表评分方面,治疗后,3组患者的NIHSS评分均较治疗前降低(P0.05),表明头针运动治疗方案的疗效与头针留针时长呈正向时效关系。(2)实验室指标方面,治疗后,3组患者的外周血CRP、FPG水平和两观察组的TG、LDL-C水平以及观察2组的HDL-C水平均较治疗前改善(P0.05)。(3)安全性方面,3组患者治疗期间均未发生晕针、断针、血肿等不良反应,且患者的生命体征均平稳,脉搏、血压、呼吸频率等均无明显异常变化。【结论】头针治疗可有效改善脑卒中患者的运动功能,且留针时长呈正向时效关系,以头针留针2 h方案疗效更显著。

关键词：脑卒中运动功能障碍头针疗法留针时间 C反应蛋白(CRP) 血糖血脂

3D Finite Element Analysis of a Man Hip Joint Femur under Impact Loads

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Chinese Journal of Biomedical Engineering(English Edition) 2007年第1期16卷 1-7页

作者： YU Xue-zhong GUO Yi-mu LI Jun ZHANG Yun-qiu HE Rong-xin Second Affiliated Hospital College of Medicine Zhejiang University Hangzhou 310009 China Department of Engineering Mechanics Zhejiang University Hangzhou 310027 China

Objective： The biomechanical characters of the bone fracture of the man femoral hip joint under impact loads are explored. Methods ：A biosystem model of the man femoral hip joint by using the GE （ General Electric） lightspeed multi-lay spiral CT is conducted. A 3D finite element model is established by employing the finite element software ANSYS. The FE analysis mainly concentrates on the effects of the impact directions arising from intense movements and the parenchyma on the femoral hip joint on the stress distributions of the proximal femur. Results：The parenchyma on the hip joint has relatively large relaxation effect on the impact loads. Conclusion：Effects of the angle δ of the impact load to the anterior direction and the angle γ of the impact load to the femur shaft on the bone fracture are given;δ has larger effect on the stress and strain distributions than the angle γ,which mainly represents the fracture of the upper femur including the femoral neck fracture when the posterolateral femur is impacted, consistent with the clinical resuits.

关键词： Femoral hip joint Impact loading 3 D finite element analysis

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电力系统中长期过程动态仿真的组合积分算法

电力自动化设备 2017年第2期37卷 113-120页

作者：张芳仇雪芳李传栋天津大学智能电网教育部重点实验室天津300072 福建省电力有限公司电力科学研究院福建福州350007

电力系统中长期过程具有非线性、强刚性的特点,其仿真时间框架长,与电力系统机电暂态过程相比,中长期过程对数值积分算法的数值稳定性、收敛性和计算效率的要求更高。针对此问题,将隐式梯形积分法和多步高阶隐式Taylor级数法进行组合,... 详细信息

电力系统中长期过程具有非线性、强刚性的特点,其仿真时间框架长,与电力系统机电暂态过程相比,中长期过程对数值积分算法的数值稳定性、收敛性和计算效率的要求更高。针对此问题,将隐式梯形积分法和多步高阶隐式Taylor级数法进行组合,提出新的组合积分算法。在机电暂态过程采用隐式梯形积分法,在中长期过程采用多步高阶隐式Taylor级数法。算例仿真与分析结果验证了新组合积分算法的有效性和可行性。

关键词：电力系统机电暂态中长期动态刚性系统新组合积分算法

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盘龙参内生真菌多样性及其拮抗作用研究

江西农业大学学报 2019年第2期41卷 365-371页

作者：裴东方吴秋秋莫俊鑫金缘罗欢邓建新周燚长江大学农学院湖北荆州434025

为研究盘龙参内生真菌的多样性,并筛选具有抑菌活性的内生真菌。通过组织分离法分离重庆合川野生盘龙参内生真菌,利用ITS序列分析进行其多样性研究,并选用3种重要农作物病原菌进行平板对峙试验,最后对拮抗效果较好的菌株进行鉴定。结果... 详细信息

为研究盘龙参内生真菌的多样性,并筛选具有抑菌活性的内生真菌。通过组织分离法分离重庆合川野生盘龙参内生真菌,利用ITS序列分析进行其多样性研究,并选用3种重要农作物病原菌进行平板对峙试验,最后对拮抗效果较好的菌株进行鉴定。结果表明:从盘龙参的根、茎、叶和花序中共分离出内生真菌48株,从中挑选出17株代表菌株,经ITS序列分析其分属于10个属,其中假埃希氏菌属真菌Pseudallescheria minutispora对番茄灰霉病菌有较好的拮抗作用,抑菌带达7.1 mm。本研究为盘龙参内生真菌的开发利用提供了基础资料,首次在国内详细描述了P. minutispora的形态学特征,为P. minutispora的分类鉴定提供依据。

关键词：盘龙参内生真菌拮抗作用 Pseudallescheria minutispora