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婴幼儿血清25-羟维生素D水平与佝偻病的关系

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实用儿科临床杂志 2007年第19期22卷 1473-1474页

作者：马兰辛德莉田悦李靖吕芳侯安存首都医科大学附属北京友谊医院儿科北京100050

目的通过对婴幼儿进行佝偻病指标调查、检测与分析,了解北京地区婴幼儿血清维生素D水平,探讨维生素D缺乏性佝偻病诊断中需注意问题。方法对北京地区2004年2月-2005年8月收治72例年龄为2-24个月的体检和就诊婴幼儿,进行临床症状询问,进... 详细信息

目的通过对婴幼儿进行佝偻病指标调查、检测与分析,了解北京地区婴幼儿血清维生素D水平,探讨维生素D缺乏性佝偻病诊断中需注意问题。方法对北京地区2004年2月-2005年8月收治72例年龄为2-24个月的体检和就诊婴幼儿,进行临床症状询问,进行体格检查,根据临床表现初步作出有无佝偻病诊断。取血查其血清25羟-维生素D[25-（OH）D]及血清钙、磷、碱性磷酸酶（ALP）和小儿骨源性碱性磷酸酶（NBAP）水平。部分小儿行左腕X线片检查。结果单纯根据临床症状、体征诊断为无佝偻病正常组婴幼儿30例,年龄（10.75±6.27）个月;判断为佝偻病者42例,年龄（9.05±5.55）个月。二组血清25-（OH）D、ALP及NBAP均无统计学差异。血清25-（OH）D水平降低者二组各有1例;较正常值增高者21例。佝偻病组无1例血清钙、磷水平降低,其平均值反而较正常组为高。二组分别有23例及31例行左腕X线摄片检查,无1例异常发现。结论北京地区大多数婴幼儿血清25-（OH）D及钙、磷水平正常。单纯以临床表现判断有无佝偻病时,既存在过度诊断问题,也有漏诊可能;应尽可能对疑诊患儿进行血清25-（OH）D水平检测,避免维生素D应用不足或过量。

关键词： 25羟-维生素D 佝偻病婴幼儿

应用巢式聚合酶链反应和测序检测肺炎支原体23S rRNA中点突变

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中华儿科杂志 2008年第7期46卷 522-525页

作者：辛德莉糜祖煌韩旭秦玲李靖刘禧杰马少杰侯安存李贵首都医科大学附属北京友谊医院儿科 100050 无锡市克隆遗传技术研究所中国石油天然气集团公司中心医院儿科

目的建立快速检测肺炎支原体（Mycoplasrna pneumoniae，MP）耐药性的实验方法并应用于临床以了解MP耐药现状。方法应用巢式PCR直接检测咽拭子标本MP-23S rRNA基因并对扩增产物进行电泳检测或DNA测序分析；通过MP培养及体外药物敏感性... 详细信息

目的建立快速检测肺炎支原体（Mycoplasrna pneumoniae，MP）耐药性的实验方法并应用于临床以了解MP耐药现状。方法应用巢式PCR直接检测咽拭子标本MP-23S rRNA基因并对扩增产物进行电泳检测或DNA测序分析；通过MP培养及体外药物敏感性试验测定临床分离株的红霉素最小抑菌浓度，以验证23S rRNA基因检测结果的可靠性。结果200例临床标本经MP-IgM抗体、咽拭子MP种特异性16S rRNA基因巢式PCR扩增和MP分离培养测定证实为MP64例。应用巢式PCR技术扩增MP-23S rRNA基因，并对扩增产物进行测序，将基因序列与基因库中MP标准株基因序列进行比较，与标准株序列相同的共计26例，其余38例存在23S rRNA点突变：35例在2063位点发生了A到G的点突变，1例在2063位点有A到C的点突变，2例在2064位点有A到G的点突变；耐药率为59．4％。MP耐药基因检测方法灵敏度达10^2ccu／ml，MP培养及药物敏感性试验证实23S rRNA基因检测结果可靠。结论建立的MP耐药基因检测方法能直接检测临床标本中的MP耐药基因。59％的受检标本23S rRNA基因发生点突变。

关键词：支原体,肺炎抗药性 RNA,核糖体,23S 聚合酶链反应

Hydrogen sulfide reduces oxidative stress in Huntington's disease via Nrf2

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Neural Regeneration Research 2025年第6期20卷 1776-1788页

作者： Zige Jiang dexiang liu Tingting li Chengcheng Gai Danqing xin Yijing Zhao Yan Song Yahong Cheng Tong li Zhen Wang department of Physiology School of Basic Medical SciencesCheeloo College of MedicineShandong UniversityJinanShandong ProvinceChina department of Medical Psychology and Ethics School of Basic Medicine SciencesCheeloo College of MedicineShandong UniversityJinanShandong ProvinceChina department of Neurosurgery Surgery Qingdao Municipal HospitalQingdaoShandong ProvinceChina

The pathophysiology of Huntington's disease involves high levels of the neurotoxin quinolinic acid. Quinolinic acid accumulation results in oxidative stress, which leads to neurotoxicity. However, the molecular and cellular mechanisms by which quinolinic acid contributes to Huntington's disease pathology remain unknown. In this study, we established in vitro and in vivo models of Huntington's disease by administering quinolinic acid to the PC12 neuronal cell line and the striatum of mice, respectively. We observed a decrease in the levels of hydrogen sulfide in both PC12 cells and mouse serum, which was accompanied by down-regulation of cystathionine β-synthase, an enzyme responsible for hydrogen sulfide production. However, treatment with NaHS(a hydrogen sulfide donor) increased hydrogen sulfide levels in the neurons and in mouse serum, as well as cystathionine β-synthase expression in the neurons and the mouse striatum, while also improving oxidative imbalance and mitochondrial dysfunction in PC12 cells and the mouse striatum. These beneficial effects correlated with upregulation of nuclear factor erythroid 2-related factor 2 expression. Finally, treatment with the nuclear factor erythroid 2-related factor 2inhibitor ML385 reversed the beneficial impact of exogenous hydrogen sulfide on quinolinic acid-induced oxidative stress. Taken together, our findings show that hydrogen sulfide reduces oxidative stress in Huntington's disease by activating nuclear factor erythroid 2-related factor 2,suggesting that hydrogen sulfide is a novel neuroprotective drug candidate for treating patients with Huntington's disease.

关键词： apoptosis cystathionine-β-synthase nuclear factor erythroid 2-related factor 2 Huntington's disease hydrogen sulfide mitochondrion neuroplasticity oxidative stress quinolinic acid reactive oxygen species

北京地区1890例莱姆病疑似患者流行病学分析

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寄生虫与医学昆虫学报 2017年第3期24卷 155-160页

作者：田秀君栗绍刚吴赵永辛德莉首都医科大学附属北京友谊医院北京热带医学研究所热带病防治研究北京市重点实验室北京100050

为了解莱姆病Lyme disease在北京地区的感染状况,应用EliSA的方法检测了2013~2015年度首都医科大学附属北京友谊医院收集的1 890例莱姆病疑似患者血清中的抗莱姆病螺旋体Ig G抗体,并对抗体阳性病例的临床资料进行了回顾性分析。综合分... 详细信息

为了解莱姆病Lyme disease在北京地区的感染状况,应用EliSA的方法检测了2013~2015年度首都医科大学附属北京友谊医院收集的1 890例莱姆病疑似患者血清中的抗莱姆病螺旋体Ig G抗体,并对抗体阳性病例的临床资料进行了回顾性分析。综合分析各抗体阳性病例的流行病学史和具体临床表现,65例抗体阳性病例中57例确诊为莱姆病患者。本研究中莱姆病抗体的检出率为3.44%,临床诊断符合率为87.69%。不同性别、年龄段患者之间莱姆病的阳性率之间无差别,不同年份和季节莱姆病的阳性率之间差异显著。本文将加深医务工作者对莱姆病的认识,提高临床医师对该病的诊治水平。

关键词：莱姆病流行病学筛查实验

Vector meson's spin alignments in high energy reactions

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 5-20页

作者： Jin-Hui Chen Zuo-Tang liang Yu-Gang Ma xin-li Sheng Qun Wang Key Laboratory of Nuclear Physics and Ion-beam Application (MoE) Institute of Modern PhysicsFudan University Institute of Frontier and Interdisciplinary Science Key Laboratory of Particle Physics and Particle Irradiation (MOE)Shandong University INFN-Firenze department of Modern Physics University of Science and Technology of China School of Mechanics and Physics Anhui University of Science and Technology

The global spin alignment of vector mesons has been observed by the STAR collaboration at the Relativistic Heavy Ion Collider （RHIC） at Brookhaven National Laboratory （BNL）.It provides a unique opportunity to probe the correlation between the polarized quark and antiquark in the strongly coupled quark-gluon plasma （sQGP） produced in relativistic heavy ion collisions,opening a new window to explore the properties of s *** addition,spin alignments of vector mesons have also been observed in other high-energy particle collisions such as e+e-annihilations at high energies where hadron production is dominated by quark fragmentation *** results obtained are quite different from those obtained in heavy ion collisions where quark coalescence/combination mechanism dominates suggesting strong dependence on hadronization *** comprehensive studies in different hadronization processes are *** this article,we present a brief review of theoretical and experimental advances in the study of vector meson’s spin alignments in a variety of high-energy particle collisions,with emphasis on hadronization mechanisms.

关键词： vector meson spin alignment global polarization relativistic heavy ion collisions fragmentation

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LAMP技术在肺炎支原体快速检测中的应用

中国医疗器械信息 2014年第7期20卷 16-18页

作者：辛德莉李丹米佳首都医科大学附属友谊医院北京热带病研究所北京100050 北京蓝谱生物科技有限公司北京100081

环介导等温扩增技术具有快速、特异、灵敏度高、操作简便等特点,本文介绍了LAMP?技术在肺炎支原体上的应用,结果表明,MP-LAMP方法具有较高特异性和灵敏度,是一种非常适用于临床检测的方法。

关键词：环介导等温扩增技术肺炎支原体快速检测

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套式PCR法在肺炎支原体感染诊断中的应用

中国医药指南 2012年第7期10卷 1-1,7页

作者：马少杰辛德莉民航总医院儿科北京100123 首都医科大学附属北京友谊医院儿科北京100050

目的比较套式PCR法(nested polymerase chain reaction,nPCR)与培养法在早期诊断MP(Mycoplasma pneumoniae,MP)感染敏感性差异。方法选取2007年至2009年间MPP(Mycoplasma pneumoniae pneumonia,MPP)患儿,分别行血清MP-IgM测定、取咽拭子标本行MP分离、鉴定及培养,提取MP-DNA样本,应用nPCR法进行分子鉴定。结果累计选取研究对象65例,nPCR法检测阳性率分别为:16SrRNA-nPCR为75.4%(49例)和23SrRNA-nPCR阳性率为78.5%(51例),无统计学差异(P=0.687);分离培养得到MP31株,培养法MP阳性率47.6%,与nPCR法相比有显著差异(P<0.001)。结论早期检测MP感染nPCR方法比培养法敏感性高。

关键词：肺炎支原体套式PCR

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北京地区肺炎支原体肺炎的流行状况

实用儿科临床杂志 2006年第16期21卷 1054-1055页

作者：辛德莉李贵李靖马红秋陈小庚首都医科大学附属北京友谊医院儿科北京100050

目的了解北京地区肺炎支原体（MP）肺炎的发病规律及感染后带菌状况。方法选择1929例儿科住院的肺炎患儿．对不同年度、不同季节、不同年龄MP肺炎的发病情况进行统计。对20例确诊为MP肺炎患儿进行随访。结果MP肺炎发病率第一、二、三季... 详细信息

目的了解北京地区肺炎支原体（MP）肺炎的发病规律及感染后带菌状况。方法选择1929例儿科住院的肺炎患儿．对不同年度、不同季节、不同年龄MP肺炎的发病情况进行统计。对20例确诊为MP肺炎患儿进行随访。结果MP肺炎发病率第一、二、三季度间比较P均〉0．05，第一、二、三季度分别与第四季度比较，P均〈0．05。7～9岁与10～14岁比较P〉0．05，1个月～3岁与4～6岁、7～9岁、10～14岁比较，4～6岁与7～9岁、10～14岁比较P均〈0．05。1992～1994年支原体肺炎呈逐年下降趋势，而1995年末发病显著增加（2、3倍），后又有下降趋势。20例MP肺炎培养结果显示，带菌时间最短为0．5个月，平均带菌时间为1．5个月，最长为4．5个月。结论MP肺炎的发病与年龄、季节和年度有密切关系。

关键词：肺炎支原体流行病学带菌时间

Differential distribution of PINK1 and Parkin in the primate brain implies distinct roles

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Neural Regeneration Research 2025年第4期20卷 1124-1134页

作者： Yanting liu Wei Huang Jiayi Wen xin Xiong Ting Xu Qi Wang Xiusheng Chen Xianxian Zhao Shihua li Xiaojiang li Weili Yang Guangdong Key Laboratory of Non-human Primate Research Key Laboratory of CNS Regeneration(Ministry of Education)Guangdong-Hongkong-Macao CNS Regeneration Institute of Jinan UniversityJinan UniversityGuangzhouGuangdong ProvinceChina

The vast majority of in vitro studies have demonstrated that PINK1 phosphorylates Parkin to work together in mitophagy to protect against neuronal ***,it remains largely unclear how PINK1 and Parkin are expressed in mammalian *** has been difficult to address because of the intrinsically low levels of PINK1 and undetectable levels of phosphorylated Parkin in small *** this issue is critical for elucidating the in vivo roles of PINK1 and ***,we showed that the PINK1 kinase is selectively expressed as a truncated form(PINK1–55)in the primate *** the present study,we used multiple antibodies,including our recently developed monoclonal anti-PINK1,to validate the selective expression of PINK1 in the primate *** found that PINK1 was stably expressed in the monkey brain at postnatal and adulthood stages,which is consistent with the findings that depleting PINK1 can cause neuronal loss in developing and adult monkey ***1 was enriched in the membrane-bound fractionations,whereas Parkin was soluble with a distinguishable *** double staining experiments showed that PINK1 and Parkin did not colocalize under physiological conditions in cultured monkey astrocytes,though they did colocalize on mitochondria when the cells were exposed to mitochondrial *** findings suggest that PINK1 and Parkin may have distinct roles beyond their well-known function in mitophagy during mitochondrial damage.

关键词： neurodegeneration Parkin Parkinson’s disease PINK1 subcellular distribution