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The NAC transcription factor LuNAC61 negatively regulates fiber development in flax (Linum usitatissimum L.)

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Journal of Integrative Agriculture 2024年第3期23卷 795-805页

作者： Dongwei xie Jing Li Wan Li lijun Sun Zhigang Dai Wenzhi Zhou Jianguang Su Jian Sun School of Life Sciences Nantong UniversityNantong 226019China Institute of Crop Cultivation and Tillage Heilongjiang Academy of Agricultural SciencesHarbin 150086China Institute of Bast Fiber Crops Chinese Academy of Agricultural SciencesChangsha 410221China Jiangsu Sanshu Biotechnology Co.Ltd. Nantong 226001China

Flax is a crucial fiber crop that exhibits excellent textile properties and serves as a model plant for investigating phloem fiber development. The regulation of multiple genes significantly influences fiber development, notably involving NAC(NAM, ATAF1/2, CUC2) transcription factors in forming the fiber secondary cell wall(SCW).Overexpression of LuNAC61 in flax resulted in sparse top meristematic zone leaves and significantly reduced stem cellulose content. Scanning electron microscopy and staining observations revealed a significant reduction in fiber bundles. β-Glucuronidase(GUS) staining analysis demonstrated high activity of the LuNAC61 promoter in the bast fibers of the flax stem. Additionally, several members of the LuPLATZ and LuCesA families exhibited significant coexpression with LuNAC61. Subcellular localization indicated the presence of LuPLATZ24 protein in the nucleus and cytoplasm, LuNAC61 protein exclusively in the nucleus, and LuCesA10 in the nucleus and endoplasmic reticulum. LuPLATZ24 positively regulates LuNAC61, whereas LuNAC61 negatively affects LuCesA10, suggesting the involvement of a metabolic network in regulating flax fiber development. In conclusion, this study provides a critical opportunity for a comprehensive and in-depth analysis of the mechanisms governing flax fiber development and the potential use of biotechnology to enhance flax fiber yield.

关键词： flax fiber development Lu NAC61 gene function gene interaction

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基于纹理的增强型3D流场绘制

计算机辅助设计与图形学学报 2009年第3期21卷 406-411页

作者：周迪斌解利军郑耀浙江大学工程与科学计算研究中心计算机科学与技术学院

提出一种基于纹理的增强型3D矢量场可视化算法,可显著地改善传统纹理法的绘制质量.首先通过对3D纹理的线性卷积运算生成具有空间相关性的卷积纹理;然后对卷积纹理进行高通滤波,以增加流面内流线之间强度的对比;最后通过体绘制方式展示3... 详细信息

提出一种基于纹理的增强型3D矢量场可视化算法,可显著地改善传统纹理法的绘制质量.首先通过对3D纹理的线性卷积运算生成具有空间相关性的卷积纹理;然后对卷积纹理进行高通滤波,以增加流面内流线之间强度的对比;最后通过体绘制方式展示3D卷积纹理.借助权重区域,该算法可以显示用户感兴趣区域或特征区域,避免卷积数据过多引起的紊乱及相互遮挡.

关键词：流场可视化线性卷积 3D矢量场体绘制

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稠油污水热能的再利用

油气田地面工程 2009年第3期28卷 3-4页

作者：解利军新疆油田公司HAFNIUM(新港)石油开发公司

新疆油田九1～九5区块每天的污水处理量为1.3×104～1.5×104m3,污水温度在52℃左右。为了对油田污水中的余热进行再利用,在建立日处理2×104m3的污水处理站时,应用了热泵技术,对污水处理站进行保温试验。经过2年的运行,热泵机组制热性... 详细信息

新疆油田九1～九5区块每天的污水处理量为1.3×104～1.5×104m3,污水温度在52℃左右。为了对油田污水中的余热进行再利用,在建立日处理2×104m3的污水处理站时,应用了热泵技术,对污水处理站进行保温试验。经过2年的运行,热泵机组制热性能稳定可靠,达到了油田余热再利用的效果。

关键词：污水热能热泵技术热效率效益分析

The OsFTIP6-OsHB22-OsMYBR57 module regulates drought response in rice

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Molecular Plant 2022年第7期15卷 1227-1242页

作者： Lijia Yang Ying Chen Liang Xu Jiaxuan Wang Haoyue Qi Jiazhuo Guo Liang Zhang Jun Shen Huanyu Wang Fan Zhang lijun xie Wenjun Zhu Peitao Lü Qian Qian Hao Yu Shiyong Song State Key Laboratory of Rice Biology Zhejiang Provincial Key Laboratory of Crop Genetic ResourcesInstitute of Crop ScienceCollege of Agriculture and BiotechnologyZhejiang UniversityHangzhou 310058China State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 311400China College of Horticulture FAFU-UCR Joint Center for Horticultural Biology and MetabolomicsHaixia Institute of Science and TechnologyFujian Agriculture and Forestry UniversityFuzhou 350002China Department of Biological Sciences and Temasek Life Sciences Laboratory National University of SingaporeSingapore 117543Singapore

Plants have evolved a sophisticated set of mechanisms to adapt to drought *** factors play crucial roles in plant responses to various environmental stimuli by modulating the expression of numerous stress-responsive ***,how the crosstalk between different transcription factor families orchestrates initiation of the key transcriptional network and the role of posttranscriptional modification of transcription factors,especially in cellular localization/trafficking in response to stress in rice,remain still largely *** this study,we isolated an Osmybr57 mutant that displays a drought-sensitive phenotype through a genetic screen for drought stress *** found that OsMYBR57,an MYB-related protein,directly regulates the expression of several key drought-related OsbZ/Ps in response to drought *** studies revealed that OsMYBR57 interacts with a homeodomain transcription factor,OsHB22,which also plays a positive role in drought *** further demonstrate that OsFTIP6 interacts with OsHB22 and promotes the nucleocytoplasmic translocation of OsHB22 into the nucleus,where OsHB22 cooperates with OsMYBR57 to regulate the expression of drought-responsive *** findings have revealed a mechanistic framework underlying the OsFTIP6-0sHB22-0sMYBR57 module-mediated regulation of drought response in *** OsFTIP6-mediated OsHB22 nucleocytoplasmic shuttling and OsMYBR57-0sHB22 regulation of OsbZIP transcription ensure precise control of expression of OsLEA3 and Rab21,and thereby regulate the response to water deficiency in rice.

关键词： OsMYBR57 OsHB22 OsFTIP6 drought response nucleocytoplasmic trafficking rice

Robust Output Feedback Control for A Class of Nonlinear Systems by Adaptive Neural Networks

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Robust Output Feedback Control for A Class of Nonlinear Syst...

过共晶A390-T6合金挤压铸造力学性能及组织研究

2009中国控制与决策会议

作者： LONG lijun,xie Chengkang School of Mathematics and System Science,Southwest University

By Nussbaum-type gain technique and adaptive neural network,a robust adaptive neural network controller is established for a class of uncertain nonlinear *** this controller,the output is regulated to a neighborhood of origin by appropriately choosing design parameters,and all signals of the closed-loop are kept *** feasibility is investigated by an illustrative simulation example.

关键词： Radial Basis Function Neural Network Output Feedback Backstepping

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热加工工艺 2014年第17期43卷 96-99页

作者：谢丽君长沙职业技术学院湖南长沙410111

研究了过共晶A390-T6合金挤压铸造成形工艺过程中压力、浇注温度、热处理工艺参数等对合金力学性能与微观组织的影响。实验结果表明：合金共晶硅组织,随着挤压压力增加,共晶硅颗粒越细小且分布也越均匀,当初晶硅大小大约为20-30 μm时... 详细信息

研究了过共晶A390-T6合金挤压铸造成形工艺过程中压力、浇注温度、热处理工艺参数等对合金力学性能与微观组织的影响。实验结果表明：合金共晶硅组织,随着挤压压力增加,共晶硅颗粒越细小且分布也越均匀,当初晶硅大小大约为20-30 μm时随着挤压压力的增加合金共晶硅组织基本保持不变;随着浇注温度的增加,共晶相不断由细小、圆整近似球状向蔷薇状、树枝状转变;在挤压压力为90 MPa时,可获得组织形态较好、结构致密的高性能铸件;在合适的热处理条件下,可以得到良好的显微组织和优良的力学性能。

关键词： A390-T6合金挤压铸造力学性能微观组织

基于MASTA汽车变速器齿轮的滚齿工艺和热处理分析

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热加工工艺 2013年第10期42卷 220-222,225页

作者：谢丽君长沙职业技术学院湖南长沙410151

利用MASTA圆柱齿轮制造模块对某汽车变速器齿轮进行滚齿工艺和热处理模拟。仿真结果表明:滚齿加工具有适应性好、连续切削、无空行程,生产率较高的工艺特点;由MASTA软件仿真计算齿轮热处理变形量的最大值约为70μm,而且热变形的变化趋... 详细信息

利用MASTA圆柱齿轮制造模块对某汽车变速器齿轮进行滚齿工艺和热处理模拟。仿真结果表明:滚齿加工具有适应性好、连续切削、无空行程,生产率较高的工艺特点;由MASTA软件仿真计算齿轮热处理变形量的最大值约为70μm,而且热变形的变化趋势呈现先增大再减小后增大的趋势。

关键词：变速器齿轮滚齿 MASTA 热处理

Neddylation is a novel therapeutic target for lupus by regulating double negative T cell homeostasis

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Signal Transduction and Targeted Therapy 2024年第2期9卷 738-750页

作者： Yun Zhang lijun Du Chenxi Wang Zhangsheng Jiang Qingchi Duan Yiping Li Zhijun xie Zhixing He Yi Sun Lin Huang Liwei Lu Chengping Wen Key Laboratory of Chinese medicine rheumatology of Zhejiang Province Research Institute of Chinese Medical Clinical Foundation and ImmunologyCollege of Basic Medical ScienceZhejiang Chinese Medical UniversityHangzhou 310053China Department of General Surgery Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China Cancer Institute(Key Laboratory of Cancer Prevention and Intervention China National Ministry of Education)of the Second Affiliated Hospital and Institute of Translational MedicineZhejiang University School of MedicineHangzhou 310029China Cancer Center of Zhejiang University Hangzhou 310029China Department of Pathology and Shenzhen Institute of Research and Innovation The University of Hong KongHong KongChina Chongqing International Institute for Immunology Chongqing 400038China

Systemic lupus erythematosus(SLE),a severe autoimmune disorder,is characterized by systemic inflammatory response,autoantibody accumulation and damage to *** dysregulation of double-negative(DN)T cells is considered as a crucial commander during ***,a significant type of protein post-translational modification(PTM),has been well-proved to regulate T cell-mediated immune ***,the function of neddylation in SLE is still ***,we reported that neddylation inactivation with MLN4924,a specific inhibitor of NEDD8-activating enzyme E1(NAE1),or genetic abrogation of Ube2m in T cells decreased DN T cell accumulation and attenuated murine lupus *** investigations revealed that inactivation of neddylation blocked Bim ubiquitination degradation and maintained Bim level in DN T cells,contributing to the apoptosis of the accumulated DN T cells in lupus *** double knockout(KO)lupus-prone mice(Ube2m-/-Bim-/-lpr)were generated and results showed that loss of Bim reduced Ube2m deficiency-induced apoptosis in DN T cells and reversed the alleviated lupus *** findings identified that neddylation inactivation promoted Bim-mediated DN T cell apoptosis and attenuated lupus ***,we also found that in SLE patients,the proportion of DN T cells was raised and their apoptosis was ***,compared to healthy groups,SLE patients exhibited decreased Bim levels and elevated Cullin1 neddylation ***,the inhibition of neddylation induced Bim-dependent apoptosis of DN T cells isolated from SLE ***,our findings provide the direct evidence about the function of neddylation during lupus,suggesting a promising therapeutic approach for this disease.

关键词： inactivation double finding

Generation of four-photon entangled states based on interaction between a three-level atom and two bimodal cavities

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商丘师范学院学报 2011年第6期27卷 24-27页

作者： ZHANG Dengyu TANG Shiqing WANG Xinwen xie lijun ZHAN Xiaogui CHEN Yinhua Department of Physics and Electronic Information Science Hengyang Normal University Hengyang 421008 China

A simple scheme is presented for generating four-photon entangled states with interaction between a three-level atom and two bimodal *** the proposed protocol,the quantum information is encoded on Fock states of the cavity *** detection of the atom can collapse the cavity made/wave function to the desired *** experimental feasibility of our proposal is also discussed.

关键词： quantum information three-level atom bimodal cavity entangled state