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Prospects and Problems for Identification of Poisonous Plants in China using DNA Barcodes

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Biomedical and Environmental Sciences 2014年第10期27卷 794-806页

作者： xie Lei WANG Ying Wei GUAN Shan Yue xie li jing LONG Xin SUN Cheng Ye College of Nature Conservation Beijing Forestry University Institute of Botany the Chinese Academy of Sciences School of Agricultural Forestry and Environmental Sciences Forest Resources Clemson University National Institute of Occupational Health and Poison Control China Center for Disease Control and Prevention

Objective Poisonous plants are a deadly threat to public health in China. The traditional clinical diagnosis of the toxic plants is inefficient, fallible, and dependent upon experts. In this study, we tested the performance of DNA barcodes for identification of the most threatening poisonous plants in China. Methods Seventy-four accessions of 27 toxic plant species in 22 genera and 17 families were sampled and three DNA barcodes （motK, rbcL, and ITS） were amplified, sequenced and tested. Three methods, Blast, pairwise global alignment （PWG） distance, and Tree-Building were tested for discrimination power. Results The primer universality of all the three markers was high. Except in the case of ITS for Hemerocollis minor, the three barcodes were successfully generated from all the selected species. Among the three methods applied, Blast showed the lowest discrimination rate, whereas PWG Distance and Tree-Building methods were equally effective. The ITS barcode showed highest discrimination rates using the PWG Distance and Tree-Building methods. When the barcodes were combined, discrimination rates were increased for the Blast method.

关键词： Poisonous plants DNA barcoding metK rbcL iTS

Laser Surface Remelting of Medium Ni-Cr Infinite Chilling Cast Iron Roll

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材料热处理学报 2004年第5B期25卷 1009-1012页

作者： YAOJian-hua ZHANGQun-li xieSong-jing ZhefiangUniversityofTechnology HangzhouChina

Laser surface remelting of medium Ni-Cr infinite chilling cast iron was performed with a continuous wave COa laser beam with the power of 7 KW under the argon shielding. The microstructural analysis of the laser remelted layer by optical microscope shows that the laser remelted layer consists of three zones, which is the melting zone, the transition zone and the heat affected zone. The size of the dendrite of the melting zone is only in the 1/10 to 1/30 range of that of the substrate. The distribution of the hardness of the laser remelted layer was detected, and the carrying capacity of rolling steel was also field-tested. The results show that both the hardness of the remelted layer and the carrying capacity all increase, especially, the carrying capacity was 50% increased compared with the substrate.

关键词：激光重熔铸铁轧辊显微结构晶粒细化硬度承载能力 Ni-Cr激冷

An Association between Immunosenescence and CD4^+CD25^+ Regulatory T Cells: A Systematic Review

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Biomedical and Environmental Sciences 2010年第4期23卷 327-332页

作者： liNG WANG YAN xie li-jing ZHU TING-TING CHANG YAN-QING MAO JIE li Clinical Immunology Laboratory The First Affiliated Hospital of Soochow University Suzhou 215006 Jiangsu China

Objective Age-related increment of the prevalence of CD4^＋CD25^＋ regulatory T （Treg） cells were described controversially, and whether such changes explain immune dysfunction in the elderly is still unclear. The aim of this systematic review is to evaluate the role of the Tregs in immunosenescence. Methods Medline and manual searches were performed to identify all published epidemiological and animal studies investigating the efficacy of the association between immunosenescence and Treg cells. Results It was founded that the frequency, phenotypic characteristics, and number/function of Tregs were altered significantly with aging. Medical conditions in individuals with advanced ageas well as apoptosis intensity of Treg cells had an impact on the accumulation of Tregs which in turn could deteriorate cytotoxic activity of CD8＋ T and NK cells and production of IL-2. The range of immune cells that could be suppressed by Treg cells was quite wide and covered CD4^＋CD25^＋ T cells, NK cells, dendritic cells and even monocytes. These changes were observed both in humans and experimental animals. Besides, it was believed that frequency of Tregs increased with age and was accompanied by intensified suppressive activity for Tregs in patients, for example, with Alzheimer disease （AD） and Parkinson disease （PD）. The impaired condition of CD4＋ T cells, so-called immunosenescence, rendered transplant recipients less responsive to an allogeneic kidney graft, an effect that was limited to transplant recipients who were aged over 60 years. Conclusions Treg cells are associated with immunosenescence. All these changes contribute to the aging-related decline of immune responses and lead to the higher risk of immune-mediated diseases, cancer or infections in aged individuals.

关键词： Aging Immunosenescence CD4^+CD25^+ T cell Treg Case-control studies Cohort studies Cross-sectional studies

GP‐FMLNet:A feature matrix learning network enhanced by glyph and phonetic information for Chinese sentiment analysis

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CAAI Transactions on Intelligence Technology 2024年第4期9卷 960-972页

作者： jing li Dezheng Zhang Yonghong xie Aziguli Wulamu Yao Zhang School of Computer and Communication Engineering University of Science and Technology BeijingBeijingChina Beijing Key Laboratory of Knowledge Engineering for Materials Science University of Science and Technology BeijingBeijingChina University of Alberta EdmontonAlbertaCanada

Sentiment analysis is a fine‐grained analysis task that aims to identify the sentiment polarity of a specified *** methods in Chinese sentiment analysis tasks only consider sentiment features from a single pole and scale and thus cannot fully exploit and utilise sentiment feature information,making their performance less than *** resolve the problem,the authors propose a new method,GP‐FMLNet,that integrates both glyph and phonetic information and design a novel feature matrix learning process for phonetic features with which to model words that have the same pinyin information but different glyph *** method solves the problem of misspelling words influencing sentiment polarity prediction ***,the authors iteratively mine character,glyph,and pinyin features from the input comments ***,the authors use soft attention and matrix compound modules to model the phonetic features,which empowers their model to keep on zeroing in on the dynamic‐setting words in various positions and to dispense with the impacts of the deceptive‐setting ***-periments on six public datasets prove that the proposed model fully utilises the glyph and phonetic information and improves on the performance of existing Chinese senti-ment analysis algorithms.

关键词： aspect‐level sentiment analysis deep learning feature extraction glyph and phonetic feature matrix compound learning

Effects of interactions between arbuscular mycorrhizal fungi and bacteria on the growth of Lotus corniculatus L.:From the perspective of regulating rhizosphere fungal community

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Pedosphere 2024年第2期34卷 411-423页

作者： Qing ZHANG Jin CHEN Yingyue li Tao HUANG Kailing xie jing ZHOU Xiaoyu li Schools of life Sciences Anhui Agricultural UniversityHefei 230036China National Engineering Laboratory of Crop Stress Resistance Breeding Anhui Agricultural UniversityHefei 230036China Anhui Province Key Laboratory of Crop Biology Anhui Agricultural UniversityHefei 230036China

Arbuscular mycorrhizal fungi(AMF)provide essential nutrients to crops and are affected by ***-solubilizing bacteria(PSB),nitrogen-fixing bacteria(NFB),and AMF have mutually beneficial relationships with plants,but the effects of their interactions on plant growth by regulating rhizosphere fungal community have not been sufficiently *** this study,a greenhouse pot experiment was conducted to investigate the interactions between AMF and bacteria(PSB and NFB)on the growth of Lotus corniculatus ***,the role of rhizosphere fungal community in the growth of Lotus corniculatus *** explored using Illumina MiSeq high-throughput *** results showed that combined inoculation of AMF with PSB and NFB increased plant biomass,plant height,and fungal colonization *** richness,complexity,and stability of rhizosphere fungal community also increased after combined inoculation of AMF with PSB and/or NFB,particularly with *** addition,combined inoculation of AMF with PSB and NFB enriched the abundance of beneficial microorganisms,with Chaetomium and Humicola showing the greatest *** structural equation model showed that the interactions of AMF with PSB and NFB promoted plant growth by affecting fungal network structure and soil enzyme activities involved in carbon,nitrogen,and phosphorus *** findings provide evidence for the effects of interactions of AMF with PSB and NFB on rhizosphere fungal community and plant growth.

关键词： colonization intensity colonization rate combined inoculation enzyme activity network structure nitrogen-fixing bacteria phosphatesolubilizing bacteria

Exploration of cyclooxygenase-2 inhibitory peptides from walnut dreg proteins based on in silico and in vitro analysis

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Food Science and Human Wellness 2024年第3期13卷 1636-1644页

作者： Zishan Hong jing xie liang Tao jing-jing Dai Tingting li li Zhang Yuying Bai Xia Hu Jinlian Chen Jun Sheng Yang Tian College of Food Science and Technology Yunnan Agricultural UniversityKunming 650201China Yunnan Provincial Key Laboratory of Precision Nutrition and Personalized Food Manufacturing Yunnan Agricultural UniversityKunming 650201China Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources Ministry of EducationYunnan Agricultural UniversityKunming 650201China School of Tea and Coffee Pu’er UniversityPu’er 665000China Yunnan Provincial Engineering Research Center for Edible and Medicinal Homologous Functional Food Yunnan Agricultural UniversityKunming 650201China Key Laboratory of Pu-er Tea Science Ministry of EducationYunnan Agricultural UniversityKunming 650201China

Walnut dreg protein hydrolysates(WDPHs)exhibit a variety of biological activities,however,the cyclooxygenase-2(COX-2)inhibitory peptide of WDPHs remain *** aim of this study was to rapidly screen for such peptides in WDPHs through a combination of in silico and in vitro *** total,1262 peptide sequences were observed by nano liquid chromatography/tandem mass spectrometry(nano LC-MS/MS)and 4 novel COX-2 inhibitory peptides(AGFP,FPGA,LFPD,and VGFP)were *** kinetic data indicated that AGFP,FPGA,and LFPD displayed mixed-type COX-2 inhibition,whereas VGFP was a non-competitive *** is mainly because the peptides form hydrogen bonds and hydrophobic interactions with residues in the COX-2 active *** results demonstrate that computer analysis combined with in vitro evaluation allows for rapid screening of COX-2 inhibitory peptides in walnut protein dregs.

关键词： Walnut dreg proteins Cyclooxygenase-2 inhibitory peptide Identification Virtual screening Molecular docking

Investigation on the cutting force of high-speed milling of high volume fraction of SiCp/Al composites with PCD tools

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Investigation on the cutting force of high-speed milling of ...

The NAC transcription factor LuNAC61 negatively regulates fiber development in flax (linum usitatissimum L.)

第八届中国功能材料及其应用学术会议

作者： PENG Song xie li-jing WANGXi-bin FU Na-xin SHI Xing-kuan Key Laboratory of Fundamental Science for Advanced Machining Beijing Institute of Technology

ISBN: (纸本)9783037859698

High-speed milling tests for 65vol%SiCp/6063A1 composites were performed by polycrystalline diamond(PCD) tools,response surface methodology was utilized in this study,and a cutting force model was developed through response surface methodology,which contained some important parameters such as cutting speed,cutting feed rate,cutting depth and cutting *** analysis of variance(ANOVA) indicated that the proposed mathematical model can adequately describe the relationship between cutting force and cutting process *** results show that cutting depth is the biggest important factor of milling force,and cutting feed rate is the second important factor,cutting speed is the third;milling force would not increase with the increasing of cutting width.

关键词： High-speedmilling 65vol%SiCp/6063Al composites PCD,Response surface methodology Cutting force

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Journal of Integrative Agriculture 2024年第3期23卷 795-805页

作者： Dongwei xie jing li Wan li lijun Sun Zhigang Dai Wenzhi Zhou Jianguang Su Jian Sun School of life Sciences Nantong UniversityNantong 226019China Institute of Crop Cultivation and Tillage Heilongjiang Academy of Agricultural SciencesHarbin 150086China Institute of Bast Fiber Crops Chinese Academy of Agricultural SciencesChangsha 410221China Jiangsu Sanshu Biotechnology Co.Ltd. Nantong 226001China

Flax is a crucial fiber crop that exhibits excellent textile properties and serves as a model plant for investigating phloem fiber development. The regulation of multiple genes significantly influences fiber development, notably involving NAC(NAM, ATAF1/2, CUC2) transcription factors in forming the fiber secondary cell wall(SCW).Overexpression of LuNAC61 in flax resulted in sparse top meristematic zone leaves and significantly reduced stem cellulose content. Scanning electron microscopy and staining observations revealed a significant reduction in fiber bundles. β-Glucuronidase(GUS) staining analysis demonstrated high activity of the LuNAC61 promoter in the bast fibers of the flax stem. Additionally, several members of the LuPLATZ and LuCesA families exhibited significant coexpression with LuNAC61. Subcellular localization indicated the presence of LuPLATZ24 protein in the nucleus and cytoplasm, LuNAC61 protein exclusively in the nucleus, and LuCesA10 in the nucleus and endoplasmic reticulum. LuPLATZ24 positively regulates LuNAC61, whereas LuNAC61 negatively affects LuCesA10, suggesting the involvement of a metabolic network in regulating flax fiber development. In conclusion, this study provides a critical opportunity for a comprehensive and in-depth analysis of the mechanisms governing flax fiber development and the potential use of biotechnology to enhance flax fiber yield.

关键词： flax fiber development Lu NAC61 gene function gene interaction

GAN-DIRNet:A Novel Deformable Image Registration Approach for Multimodal Histological Images

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Computers, Materials & Continua 2024年第7期80卷 487-506页

作者： Haiyue li jing xie jing Ke Ye Yuan Xiaoyong Pan Hongyi Xin Hongbin Shen Institute of Image Processing and Pattern Recognition Shanghai Jiao Tong Universityand Key Laboratory of System Control and Information ProcessingMinistry of Education of ChinaShanghai200240China Department of Pathology Ruijin HospitalShanghai Jiao Tong UniversitySchool of MedicineShanghai200025China Department of Computer Science and Engineering Shanghai Jiao Tong UniversityShanghai200240China Global Institute of Future Technology Shanghai Jiao Tong UniversityShanghai200240China

Multi-modal histological image registration tasks pose significant challenges due to tissue staining operations causing partial loss and folding of *** neural network(CNN)and generative adversarial network(GAN)are pivotal inmedical image ***,existing methods often struggle with severe interference and deformation,as seen in histological images of conditions like Cushing’s *** argue that the failure of current approaches lies in underutilizing the feature extraction capability of the discriminator *** this study,we propose a novel multi-modal registration approach GAN-DIRNet based on GAN for deformable histological image *** begin with,the discriminators of two GANs are embedded as a new dual parallel feature extraction module into the unsupervised registration networks,characterized by implicitly extracting feature descriptors of specific ***,modal feature description layers and registration layers collaborate in unsupervised optimization,facilitating faster convergence and more precise ***,experiments and evaluations were conducted on the registration of the Mixed National Institute of Standards and Technology database(MNIST),eight publicly available datasets of histological sections and the Clustering-Registration-Classification-Segmentation(CRCS)dataset on the Cushing’s *** results demonstrate that our proposed GAN-DIRNet method surpasses existing approaches like DIRNet in terms of both registration accuracy and time efficiency,while also exhibiting robustness across different image types.

关键词： Histological images registration deformable registration generative adversarial network cushing’s disease machine learning computer vision