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Screening and identification of HTNV_(pv)entry inhibitors with high-throughput pseudovirus-based chemiluminescence

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Virologica Sinica 2022年第4期37卷 531-537页

作者： xiaojing Wen Li Zhang Qiang Liu xinyue xiao Weijin Huang Youchun Wang Division of HIV/AIDS and Sex-transmitted Virus Vaccines Institute for Biological Product ControlNational Institutes for Food and Drug Control(NIFDC)and WHO Collaborating Center for Standardization and Evaluation of BiologicalsBeijing 102629China Department of Microbiological Laboratory Technology School of Public HealthShandong UniversityJinan 250012China Institute for Reference Standards and Standardization National Institutes for Food and Drug ControlBeijing 102629China

Hantaviruses,such as Hantaan virus(HTNV)and Seoul virus,are the causative agents of Hantavirus cardiopulmonary syndrome(HCPS)and hemorrhagic fever with renal syndrome(HFRS),and are important zoonotic *** has the highest incidence of HFRS,which is mainly caused by HTNV and Seoul *** approved antiviral drugs are available for these hantaviral ***,a chemiluminescence-based highthroughput-screening(HTS)assay was developed and used to screen HTNV pseudovirus(HTNVpv)inhibitors in a library of 1813 approved drugs and 556 small-molecule compounds from traditional Chinese medicine *** identified six compounds with in vitro anti-HTNVpvactivities in the low-micromolar range(EC50values of0.1–2.2μmol/L;selectivity index of 40–900).Among the six selected compounds,cepharanthine not only showed good anti-HTNVpvactivity in vitro but also inhibited HTNVpv-fluc infection in Balb/c mice 5 h after infection by94%(180 mg/kg/d,P<0.01),93%(90 mg/kg/d,P<0.01),or 92%(45 mg/kg/d,P<0.01),respectively,in a bioluminescent imaging mouse model.A time-of-addition analysis suggested that the antiviral mechanism of cepharanthine involves the membrane fusion and entry ***,we have established a HTS method for antiviral drugs screening,and shown that cepharanthine is a candidate for HCPS and HFRS *** findings may offer a starting point for the treatment of patients infected with hantaviruses.

关键词： Hantaan virus(HTNV) High-throughput screening(HTS) Inhibitor Drug repurposing

Study on a noninvasive method for rapid screening Human Serum albumin injectables by Raman spectroscopy

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Journal of Innovative Optical Health Sciences 2017年第1期10卷 34-42页

作者： Yu Zhao Nan Ji Xiuji Li Jifeng Hou Min Li Yongjian Yang Ping Tian Xinmei Sun xinyue xiao Lihui Yin National Institutes of Food and Drug Control(NIFDC)No.2 Tiantan Xi Li BeijingP.R.China tSichuan Vspec Technologies Co.Ltd.Beijing Office No.2 BeiTaiPingZhuang RoadBeijingP.R.China Shanghai Institute for Food and Drug Control(SIFDC)No.1500 Zhangheng Road ShanghaiP.R.China

Human serum albumin(HSA)injectable product is a severely afflicted area on drug safety due to its high price and restricted *** spectroscopy performances high specificity on HSA detection and it is even possible to determine HSA injectable products *** this study,we developed a noninvasive rapid screening method for of HSA injectable products by using portable Raman *** models were established by using principal component analysis combined with classical least squares(PCA-CLS)algorithm,while quanti-tative model was established by using partial least squares(PLS)*** transfer in different instruments of both the same and different apparatus modules was further discussed in this paper.A total of 34 HSA injectable samples collected from markets were used for *** identification results showed 100%accuracy and the predicted concentrations of those identified as true HSA were consistent with their labeled *** quantitative results also indicated that model transfer was excellent in the same apparatus modules of Raman spectrometer at all concentration levels,and still good enough in the different apparatus modules although the relative standard deviation(RSD)value showed a little increasing trend at low HSA concentration *** conclusion,the method was proved to be feasible and efficient for screening HSA injections,especially on its screening speed and the consideration of glass ***,with inspiring results on the model transfer,the method could be used as a universal screening mean to different Raman instruments.

关键词： Human serum albumin Raman noninvasive rapid screening method principal component analysis combined with classical least squares partial least squares model transfer.

Screening and Identification of Marburg Virus Entry Inhibitors Using Approved Drugs

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Virologica Sinica 2020年第2期35卷 235-239页

作者： Li Zhang Shan Lei Hui Xie Qianqian Li Shuo Liu Qiang Liu Weijin Huang xinyue xiao Youchun Wang Division of HIV/AIDS and Sexually-transmitted Virus Vaccines National Institutes for Food and Drug ControlBeijing 102629China Institute for Reference Standards and Standardization National Institutes for Food and Drug ControlBeijing 102629China

Dear Editor,Marburg virus(MARV)belongs to the Filoviridae family,along with the related Ebola virus(EBOV).Although MARV is less renowned than EBOV,it causes an equally devastating disease,with clinical symptoms similar to EBOV infection and a remarkably high mortality *** date,about 600 MARV cases have been reported globally,with an average mortality rate of around 80%(Brauburger et al.2012).Due to the severity of disease caused by MARV,as well as its threat to global public health,MARV is classified as a risk group 4 pathogen by WHO,necessitating containment measures equivalent to biosafety level 4(BSL-4).

关键词： mortality al. clinical

Modulating the threshold voltage of oxide nanowire field-effect transistors by a Ga^＋ ion beam

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Nano Research 2014年第11期7卷 1691-1698页

作者： Wenqing Li Lei Liao Xiangheng xiao xinyue Zhao Zhigao Dai Shishang Guo Wei Wu Ying Shi Jinxia Xu Feng Ren Changzhong Jiang Department of Physics and Key Laboratory of Artificial Micro- and Nano-structures of Ministry of Education Wuhan UniversityWuhan 430072 China

In this paper, we report a method to change the threshold voltage of SnO2 and In2O3 nanowire transistors by Ga^＋ ion irradiation. Unlike the results in earlier reports, the threshold voltages of SnO2 and In2O3 nanowire field-effect transistors （FETs） shift in the negative gate voltage direction after Ga^＋ ion irradiation. Smaller threshold voltages, achieved by Ga^＋ ion irradiation, are required for high-performance and low-voltage operation. The threshold voltage shift can be attributed to the degradation of surface defects caused by Ga＋ ion irradiation. After irradiation, the current on/off ratio declines slightly, but is still close to -106. The results indicate that Ga^＋ ion beam irradiation plays a vital role in improving the performance of oxide nanowire FETs.

关键词： nanowire field-effect transistor ion irradiation threshold voltage

Quantification of Central and Eastern China's atmospheric CH_(4) enhancement changes and its contributions based on machine learning approach

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Journal of Environmental Sciences 2024年第4期138卷 236-248页

作者： xinyue Ai Cheng Hu Yanrong Yang Leying Zhang Huili Liu Junqing Zhang Xin Chen Guoqiang Bai Wei xiao College of Biology and the Environment Joint Center for sustainable Forestry in Southern ChinaNanjing Forestry UniversityNanjing 210037China Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters(CIC-FEMD) Nanjing University of Information Science&TechnologyNanjing 210044China Guang’an Vocational&Technical College Guangan 638550China HuaNan Meteorological Administration Huanan 154400China

Methane is the second largest anthropogenic greenhouse gas,and changes in atmospheric methane concentrations can reflect the dynamic balance between its emissions and ***,the monitoring of CH_(4) concentration changes and the assessment of underlying driving factors can provide scientific basis for the government’s policy making and *** is the world’s largest emitter of anthropogenic ***,due to the lack of ground-based observation sites,little work has been done on the spatial-temporal variations for the past decades and influencing factors in China,especially for areas with high anthropogenic emissions as Central and Eastern *** to quantify atmospheric CH_(4) enhancements trends and its driving factors in Central and Eastern China,we combined the most up-to-date TROPOMI satellite-based column CH_(4)(xCH_(4))concentration from 2018 to 2022,anthropogenic and natural emissions,and a random forest-based machine learning approach,to simulate atmospheric xCH_(4) enhancements from 2001 to *** results showed that(1)the random forest model was able to accurately establish the relationship between emission sources and xCH_(4) enhancement with a correlation coefficient(R^(2))of 0.89 and a root mean-square error(RMSE)of 11.98 ppb;(2)The xCH_(4) enhancement only increased from 48.21±2.02 ppb to 49.79±1.87 ppb from the year of 2001 to 2018,with a relative change of 3.27%±0.13%;(3)The simulation results showed that the energy activities and waste treatment were the main contributors to the increase in xCH_(4) enhancement,contributing 68.00% and 31.21%,respectively,and the decrease of animal ruminants contributed-6.70% of its enhancement trend.

关键词： TROPOMI Methane column concentrations Anthropogenic sources Random Forest model

不同覆盖措施对旱区春小麦产量及碳效率的影响

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寒旱农业科学 2024年第6期3卷 515-521页

作者：王小燕逄蕾路建龙胡南南杨锦肖歆玥张正伟任磊甘肃省干旱生境作物学重点实验室甘肃兰州730070 甘肃农业大学农学院甘肃兰州730070 甘肃农业大学植物生产类实验教学中心甘肃兰州730070 甘肃农业大学水利水电工程学院甘肃兰州730070

为实现旱区春小麦种植生产环境效益和经济效益双赢,依托大田试验,研究不同覆盖措施秸秆带状覆盖、地膜覆盖、秸秆碎秆覆盖和对照露地平作对旱区春小麦产量及碳效率的影响,并对经济效益进行了评价。结果表明,与露地平作相比,地膜覆盖显... 详细信息

为实现旱区春小麦种植生产环境效益和经济效益双赢,依托大田试验,研究不同覆盖措施秸秆带状覆盖、地膜覆盖、秸秆碎秆覆盖和对照露地平作对旱区春小麦产量及碳效率的影响,并对经济效益进行了评价。结果表明,与露地平作相比,地膜覆盖显著提高了生育期春小麦干物质积累量,增幅为37.58%;秸秆带状覆盖提高了小麦开花期和收获期干物质积累量,增幅分别为32.65%和9.83%。秸秆碎秆覆盖、地膜覆盖千粒重较露地平作分别提高了15.55%、21.91%。地膜覆盖碳投入总量增幅最大,较露地平作增加1223.07 kg C/hm^(2)。秸秆碎秆覆盖较露地平作籽粒碳和植株碳产出量分别提高了38.41%和23.34%。秸秆带状覆盖提高了碳生产效率、碳经济效率、碳生态效率,增幅分别为0.58%、0.45%、0.24%。秸秆碎秆覆盖经济效益为3882.69元,较露地平作增加22.7%。综合表明,各覆盖措施均能提高春小麦产量和经济效益,其中秸秆带状覆盖显著提高了碳生产效率、碳经济效率和碳生态效率。

关键词：秸秆带状覆盖地膜覆盖秸秆碎秆覆盖产量碳效率

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Conservation genomics of the critically endangered Chinese pangolin

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Science China Life Sciences 2024年第10期67卷 2051-2061页

作者： Shichao Wei Huizhong Fan Wenliang Zhou Guangping Huang Yan Hua Shibao Wu xiao Wei Yiting Chen xinyue Tan Fuwen Wei Jiangxi Province Key Laboratory of Conservation Biology College of ForestryJiangxi Agricultural University Center for Evolution and Conservation Biology Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) Institute of Zoology Chinese Academy of Sciences Guangdong Provincial Key Laboratory of Silviculture Protection and UtilizationGuangdong Academy of Forestry School of Life Science South China Normal University Terrestrial Wildlife Rescue and Epidemic Diseases Surveillance Center of Guangxi

The Chinese pangolin(Manis pentadactyla, MP) has been extensively exploited and is now on the brink of extinction, but its population structure, evolutionary history, and adaptive potential are unclear. Here, we analyzed 94 genomes from three subspecies of the Chinese pangolin and identified three distinct genetic clusters(MPA, MPB, and MPC), with MPB further divided into MPB1 and MPB2 subpopulations. The divergence of these populations was driven by past climate change. For MPB2 and MPC, recent human activities have caused dramatic population decline and small population size as well as increased inbreeding, but not decrease in genomic variation and increase in genetic load probably due to strong gene flow; therefore, it is crucial to strengthen in situ habitat management for these two populations. By contrast, although human activities have a milder impact on MPA, it is at high risk of extinction due to long-term contraction and isolation, and genetic rescue is urgently needed. MPB1 exhibited a relatively healthy population status and can potentially serve as a source population. Overall, our findings provide novel insights into the conservation of the Chinese pangolin and biogeography of the mammals of eastern Asia.

关键词： Manis pentadactyla conservation genomics demographic history human activities conservation strategies

Recent advances in design of hydrogen evolution reaction electrocatalysts at high current density:A review

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Chinese Journal of Catalysis 2024年第8期63卷 33-60页

作者： Zhipeng Li xiaobin Liu Qingping Yu xinyue Qu Jun Wan Zhenyu xiao Jingqi Chi Lei Wang Key Laboratory of Eco-chemical Engineering International Science and Technology Cooperation Base of Eco-chemical Engineering and Green Manufac-turingCollege of Environment and Safety EngineeringQingdao University of Science and TechnologyQingdao 266042ShandongChina College of Chemistry and Molecular Engineering Qingdao University of Science and TechnologyQingdao 266042ShandongChina

The electrolysis of water powered by renewable energy sources offers a promising method of"green hydrogen"production,which is considered to be at the heart of future carbon-neutral energy *** the past decades,researchers have reported a number of hydrogen evolution reaction(HER)electrocatalysts with activity comparable to that of commercial Pt/C,but most of them are tested within a small current density range,typically no more than 500 mA cm^(-2).To realize the industrial application of hydrogen production from water electrolysis,it is essential to develop high-efficiency HER electrocatalysts at high current density(HCD≥500 mA cm^(-2)).Nevertheless,it remains challenging and significant to rational design HCD electrocatalysts for *** this paper,the design strategy of HCD electrocatalysts is discussed,and some HCD electrocatalysts for HER are reviewed in seven categories(alloy,metal oxide,metal hydroxide,metal sulfide/selenide,metal nitride,metal phosphide and other derived electrocatalysts).At the end of this article,we also pro-pose some viewpoints and prospects for the future development and research directions of HCD electrocatalysts for HER.

关键词： Electrocatalyst High current density Hydrogen evolution reaction Water electrolysis

Programmable double-unlock nanocomplex self-supplies phenylalanine ammonia-lyase for precise phenylalanine deprivation of tumors

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Chinese Chemical Letters 2024年第8期35卷 455-459页

作者： Chunqing Ou Meijia xiao xinyue Zheng Xianzhou Huang Suleixin Yang Yingying Leng xiaowei Liu Xiuqi Liang Linjiang Song Yanjie You Shaohua Yao Changyang Gong Department of Biotherapy Cancer Center and State Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengdu 610041China State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoAvenida da UniversidadeTaipaMacao 999087China School of Medical and Life Sciences Chengdu University of Traditional Chinese MedicineChengdu 611137China Department of Gastroenterology People’s Hospital of Ningxia Hui Autonomous RegionYinchuan 750002China

Essential amino acids(EAAs)deprivation is a potential antitumor approach because EAAs are critical for tumor *** efficiently inhibit tumor growth,continuous deprivation of EAAs is required,how-ever,continuous deprivation without precise control will introduce toxicity to normal ***,a programmable double-unlock nanocomplex(ROCK)was prepared,which could self-supply phenylalanine ammonia-lyase(PAL)to tumor cells for phenylalanine(Phe)*** was double-locked in physiological conditions when administered *** ROCK actively targeted to tumor cells by integrinαvβ3/5 and CD44,ROCK was firstly unlocked by cleavage of protease on tumor cell membrane,exposing CendR and R8 to enhance ***,hyaluronic acid was digested by hyaluronidase overexpressed in endo/lysosome of tumor cells,in which ROCK was secondly unlocked,resulting in pro-moting endo/lysosome escape and PAL plasmid(pPAL)*** pPAL could sustainably express PAL in host tumor cells until the self-supplied PAL precisely and successfully deprived Phe,thereby block-ing the protein synthesis and killing tumor cells ***,our precise Phe deprivation strategy effectively inhibited tumor growth with no observable toxicity to normal cells,providing new insights to efficiently remove intratumoral nutrition for cancer therapy.

关键词： Programmable double-unlock Essential amino acids deprivation Phenylalanine ammonia-lyase Self-supply Gene therapy