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Effects of subchronic oral toxic metal exposure on the intestinal microbiota of mice

Science Bulletin 2017年第12期62卷 831-840页

作者： Qixiao Zhai Tianqi Li Leilei Yu Yue xiao Saisai Feng Jiangping Wu {2. Zhao Hao Zhang Wei chen State Key Laboratory of Food Science and Technology School of Food Science and TechnologyJiangnan UniversityWuxi 214122.China UK-China Joint Centre on Probiotic Bacteria NorwichUK and WuxiChina Beijing Innovation Centre of Food Nutrition and Human Health Beijing Technology&Business UniversityBeijing 100048.China

Oral exposure to toxic metals such as cadmium （Cd）, lead （Pb）, copper （Cu） and aluminum （AI） can induce various adverse health effects in humans and animals. However, the effects of these metals on the gut microbiota have received limited attention. The present study demonstrated that long-term toxic metal exposure altered the intestinal microbiota of mice in a metal-specific and time-dependent manner. Subchronic oral Cu exposure for eight weeks caused a profound decline in gut microbial diversity in mice, whereas no significant changes were observed in groups treated with other metals. Cd exposure signif- icantly increased the relative abundances of organisms from the genera Alistipes and Odoribacter and caused marked decreases in Mollicutes and unclassified Ruminococcaceae. Pb exposure significantly decreased the abundances of eight genera： unclassified and uncultured Ruminococcaceae, unclassified Lachnospiraceae, Ruminiclostridium_9, Rikenellaceae_RCg_gut group, Oscillibacter, Anaerotruncus and Lachnoclostridium. Cu exposure affected abundances of the genera Alistipes, Bacteroides, Ruminococcaceae_UCG-014, AUobaculum, Mollicutes_RF9_norank, Rikenellaceae_RCg gut_group, Ruminococcaceae_unclassified and Turicibacter. A1 exposure increased the abundance of Odoribacter and decreased that of Anaerotruncus. Exposure to any metal for eight weeks significantly decreased the abun- dance of Akkermansia. These results provide a new understanding regarding the role of toxic metals in the pathogenesis of intestinal and systemic disorders in the host within the gut microbiota framework.

关键词： CadmiumLeadAluminumCopperGut microbiotaMetal

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北部湾盆地油气成藏规律与勘探新领域、新类型、资源潜力

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石油学报 2024年第1期45卷 202-225页

作者：邓勇胡德胜朱继田刘国昌陈奎童传新张道军徐新德满勇游君君满晓吴云鹏周刚张建新油气资源与工程全国重点实验室中国石油大学(北京)北京102249 中海石油(中国)有限公司湛江分公司广东湛江524057

北部湾盆地是南海西部海域重要的原油勘探区和产区,具备多凹陷含油、多层系成藏、多种油气成藏模式等优势成藏条件,近年来在页岩油、潜山、岩性、深层油气、后备凹陷/洼陷等新领域、新类型油气勘探中取得了突破。为了落实北部湾盆地重... 详细信息

北部湾盆地是南海西部海域重要的原油勘探区和产区,具备多凹陷含油、多层系成藏、多种油气成藏模式等优势成藏条件,近年来在页岩油、潜山、岩性、深层油气、后备凹陷/洼陷等新领域、新类型油气勘探中取得了突破。为了落实北部湾盆地重要生烃凹陷的区域成藏规律,指导后续常规油气精细勘探开发及新领域、新类型油气勘探,对区域石油地质条件开展了研究,总结了成藏规律。研究结果表明,多期构造运动控制了北部湾盆地的构造演化和物质充填;古近系始新统流沙港组二段油页岩和半深湖亚相泥岩为盆地最重要的烃源岩,此外,在流沙港组三段、流沙港组一段、涠洲组二段也发育多套烃源岩;发育潜山储层和碎屑岩储层,形成了多源、多储、多盖的格局;凹陷区原生近源成藏、凹中断裂带/断隆带/陡坡带垂向成藏、斜坡区过路成藏、凸起/隆起区汇流成藏等多种成藏模式控制了北部湾盆地的多凹陷成藏;油气在多凹多带有序分布,油气的差异聚集控制了内气外油、深气浅油的油气分布特征,不同区带内的油气藏类型差异显著。在区域石油地质条件与成藏规律的指导下,从资源潜力、成藏条件、勘探开发程度综合分析,页岩油、潜山、岩性圈闭、深层油气、后备凹陷/洼陷是北部湾盆地未来勘探开发的重要领域。

关键词：北部湾盆地新领域新类型成藏条件资源潜力

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基于MetaCrypto平台的SM4密码算法实现

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南昌大学学报（工科版） 2024年第2期46卷 185-192页

作者：肖超恩许弘可郑玉崝王建新张磊程心雨北京电子科技学院北京100070

针对现有通用编程语言在实现密码算法时存在编程难度大和可维护性差等问题,提出了一种基于MetaCrypto平台的密码算法实现方式。基于MetaCrypto平台的MCL元语言和映射原理,实现了SM4密码算法模型和Python语言映射接口,完成了从SM4密码算... 详细信息

针对现有通用编程语言在实现密码算法时存在编程难度大和可维护性差等问题,提出了一种基于MetaCrypto平台的密码算法实现方式。基于MetaCrypto平台的MCL元语言和映射原理,实现了SM4密码算法模型和Python语言映射接口,完成了从SM4密码算法模型到Python语言的代码生成;对映射为Python平台的SM4算法进行正确性验证和加/解密速率测试,并比较了x86架构、ARM架构、LoongArch架构下的加/解密速率。实验结果显示MetaCrypto平台输出的SM4密码算法结果与官方参考文档的测试向量完全相同,验证了其正确性;在实现加/解密速率方面,基于64 Kbit的数据量在x86架构CPU下达到了0.32. Mbit·s^(-1),在ARM架构CPU下达到了0.2.4 Mbit·s^(-1),在LoongArch架构CPU下达到了0.145 Mbit·s^(-1),与GMSSL-Python实现库基本一致。

关键词： SM4密码算法 MetaCrypto平台 Python语言映射接口代码映射

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The age at first consumption of forage in calves and its effect on growth and rumination in the short‑and long‑term

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畜牧与生物技术杂志:英文版 2023年第5期14卷 2143-2157页

作者： jianxin xiao Tianyu {2. Rong Peng Gibson Maswayi Alugongo Hui Yang Muhammad Zahoor Khan Shuai Liu Yulin Ma Jingjun Wang Wei Wang Yajing Wang Shengli Li Zhijun Cao Key Laboratory of Low Carbon Culture and Safety Production in Cattle in Sichuan Animal Nutrition InstituteSichuan Agricultural UniversityChengdu 611130People’s Republic of China State Key Laboratory of Animal Nutrition International Calf and Heifer OrganizationCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193People’s Republic of China

Background Previous investigations into the effect of dietary forage on calf performance have been inconsistent,and there is a paucity of information exploring the effect of age on the growth performance and rumination of ***-four female Holstein calves(41.5±4.2.kg)were enrolled at birth,a subset of the calves were fed calf starter only(CON,n=2.)while the rest(n=63)were classified into three treatment groups:the early(EHAY,n=2.,5.1±0.8 d),the middle(MHAY,n=2.,7.9±0.8 d)and the late(LHAY,n=16,12.1.1.4 d)hay *** short-term effect of the age at first forage consump-tion(AFF)on calves’feed intake was monitored until d *** addition,the long-term effects of AFF on body weight,structural growth and rumination behavior were recorded until d *** samples were collected on d 1,7,35,84 and 196 to ana-lyze the rumen fermentation,while fecal samples were collected from d 78 to 84 to estimate digestibility *** Treatment had no effect on feed ***,the EHAY calves tended to have lower BW and ADG compared to LHAY and CON *** total-tract apparent digestibility parameters and digestible nutrients intake were significantly lower in EHAY calves compared with CON and LHAY *** in the EHAY group tended to begin ruminating ealier,while CON calves were the latest(12.3 vs.15.5 days of age).A treatment and time interaction was present for rumination time due to greater rumination in calves consuming hay compared to CON calves in week 10 to 12.the differences in rumination disappeared afterwards,no long-lasting significant differences in the rumina-tion and rumen fermentation parameters were found between *** In conclusion,this study showed that hay consumption earlier in life(in the first week,around 5 days of life)could negatively affect the growth of the calf in the short and long *** to consuming hay from the second week(around 12.days of life)or feeding concentrate only without hay,starting

关键词： Age at first forage consumption Dairy calf Growth Nutrient digestibility Rumen fermentation Rumination

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Ameliorative effects of Lactobacillus fermentum isolated from individuals following vegan， omnivorous and high-meat diets on ulcerative colitis in mice

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Food Science and Human Wellness 2023年

作者： Qingsong Zhang Wei xiao Leilei Yu Fengwei Tian jianxin Zhao Hao Zhang Wei {2. Qixiao Zhai School of Food Science and Technology， Jiangnan university National Engineering Research Center for Functional Food， Jiangnan university Wuxi Translational Medicine Research Center and Jiangsu Translational Medicine Research Institute Wuxi Branch State Key Laboratory of Food Science and Technology， Jiangnan university

Lactobacillus spp. can be beneficial for the prevention or treatment of ulcerative colitis (UC). In this study， 153 participants who followed vegan， omnivorous， or high-meat diet were recruited. Compositional analysis of the Lactobacillus community in feces revealed that L. fermentum strains were significantly affected by diet. Administration of mixed L. fermentum strains from vegans significantly improved inflammation compared to that from omnivores and high-meat consumers， as evidenced by a significant reduction in colonic tissue damage， improvement in inflammatory cytokines， enhanced expression of ZO-1， occludin， and claudin-3， and a significant increase in short chain fatty acids (SCFAs) concentration. The effect of a single strain of L. fermentum was similar to that of a mixed strains of L. fermentum group. Genomic analysis suggested that L. fermentum strains from the guts of vegans possessed a higher prevalence of genes involved in carbohydrate catabolism than those from the guts of omnivores and high-meat eaters. In particular， the ME2.gene is involved in the biosynthesis of acetate， a compound considered to possess anti-inflammatory properties. In conclusion， this study indicates strain-specific differences in the ability of L. fermentum strains to alleviate UC in mice， influenced by habitual diets.

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人工虎骨粉与玻璃酸钠联合治疗肝肾亏虚型膝骨关节炎的效果分析

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同济大学学报（医学版） 2024年第4期45卷 536-542页

作者：蒋建新郑宪友王骁陈元元李莉虞申葛京化赵佳宁上海交通大学医学院附属第六人民医院骨科上海200233 上海中医药大学附属龙华医院风湿科上海200032 上海交通大学医学院附属第六人民医院医学检验科上海200233 上海中医药大学附属龙华医院骨伤科上海200032

目的观察人工虎骨粉与玻璃酸钠联合治疗肝肾亏虚型膝骨关节炎的临床疗效。方法按随机数字表法将12.例肝肾亏虚型膝骨关节炎患者分为对照组和治疗组,每组60例。对照组患者每周进行一次玻璃酸钠关节腔内注射治疗,治疗组患者在对照组基础... 详细信息

目的观察人工虎骨粉与玻璃酸钠联合治疗肝肾亏虚型膝骨关节炎的临床疗效。方法按随机数字表法将12.例肝肾亏虚型膝骨关节炎患者分为对照组和治疗组,每组60例。对照组患者每周进行一次玻璃酸钠关节腔内注射治疗,治疗组患者在对照组基础上给予人工虎骨粉口服治疗。持续治疗5周后,比较两组治疗前后Lequesne评分、WOMAC评分、膝关节Lysholm评分、膝骨性关节炎症状分级量化表、中医证候评分,采用ELISA法检测两组治疗前后关节液中MMP-3、TIMP-1、IL-1β及TGF-β1水平。结果治疗组总有效率为86.67%,总满意度91.67%,高于对照组(P1β表达水平显著降低(P1、TGF-β1水平较治疗前升高(P1β水平,升高TIMP-1、TGF-β1的水平相关。

关键词：人工虎骨粉玻璃酸钠肝肾亏虚型膝骨关节炎

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CEPC Technical Design Report

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Radiation Detection Technology and Methods 2024年第1期8卷 I0003-I0016,1-1091页

作者： Waleed Abdallah Tiago Carlos Adorno de Freitas Konstantin Afanaciev Shakeel Ahmad Ijaz Ahmed xiaocong Ai Abid Aleem Wolfgang Altmannshofer Fabio Alves Weiming An Rui An Daniele Paolo Anderle Stefan Antusch Yasuo Arai Andrej Arbuzov Abdesslam Arhrib Mustafa Ashry Sha Bai Yu Bai Yang Bai Vipul Bairathi Csaba Balazs Philip Bambade Yong Ban Tripamo Bandyopadhyay Shou-Shan Bao Desmond P.Barber Ayse Bat Varvara Batozskaya Subash Chandra Behera Alexander Belyaev Michele Bertucci xiao-Jun Bi Yuanjie Bi Tianjian Bian Fabrizio Bianchi Thomas Biekotter Michela Biglietti Shalva Bilanishvili Deng Binglin Denis Bodrov Anton Bogomyagkov Serge Bondarenko Stewart Boogert Maarten Boonekamp Marcello Borri Angelo Bosotti Vincent Boudry Mohammed Boukidi Igor Boyko Ivanka Bozovic Giuseppe Bozzi Jean-Claude Brient Anastasiia Budzinskaya Masroor Bukhari Vladimir Bytev Giacomo Cacciapaglia Hua Cai Wenyong Cai Wujun Cai Yijian Cai Yizhou Cai Yu{2. Cai Haiying Cai Hua{2.g Cai Lorenzo Calibbi Junsong Cang Guofu Cao Jianshe Cao Antoine Chance Xuejun Chang Yue Chang Zhe Chang Xinyuan Chang Wei Chao Auttakit Chatrabhuti Yimin Che Yuzhi Che Bin {2. Danping {2. Fuqing {2. Fusan {2. Gang {2. Guoming {2. Hua-Xing {2. Huirun {2. Jinhui {2. Ji-Yuan {2. Kai {2. Mali {2. Mingjun {2. Mingshui {2. Ning {2. Shanhong {2. Shanzhen {2. Shao-Long {2. Shaomin {2. Shiqiang {2. Tianlu {2. Wei {2. Xiang {2. xiaoyu {2. Xin {2. Xun {2. Xurong {2. Ye {2. Ying {2. Yukai {2. Zelin {2. Zilin {2. Gang {2. Boping {2. Chunhui {2. Hok Chuen {2.g Huajie {2.g Shan {2.g Tongguang {2.g Yunlong Chi Pietro Chimenti Wen Han Chiu Guk Cho Ming-Chung Chu xiaotong Chu Ziliang Chu Guglielmo Coloretti Andreas Crivellin Hanhua Cui xiaohao Cui Zhaoyuan Cui Brunella D'Anzi Ling-Yun Dai Xin{2. Dai Xuwen Dai Antonio De Maria Nicola De Filippis Christophe De La Taille Francesca De Mori Chiara De Sio Elisa Del Core Shuangxue Deng Wei-Tian Deng Zhi Deng Ziyan Deng Bhupal Dev Tang Dewen Biagio Di Micco Ran Ding Siqin Dingl Yadong Ding Haiyi Dong Jianin AGH university of Science and Technology Faculty of Physics and Applied Computer ScienceKrakow Anhui university HefeiAnhui Applied Physics Department Federal Urdu University of ArtsScience and TechnologyIslamabad Banaras Hindu university Institute of ScienceVaranasi Bandirma Onyedi Eylul university Bandirma Beihang university Beijing Beijing Computational Science Research Center Beijing Beijing Conveyi LTD Beijing Beijing Glass Research Institute Co. LtdBeijing Beijing Institute of Technology Beijing Beijing Normal university Beijing Brown university ProvidenceRI Budker Institute of Nuclear Physics Novosibirsk Cadi Ayyad university Laboratory of Fundamental and Applied PhysicsPolydisciplinary FacultyMarrakech Cairo university Giza Center for Theory and Computation National Tsing Hua UniversityHsinchu Central China Normal university WuhanHubei Central South university ChangshaHunan Centro de Fisica Teorica de Particulas Universidade Tecnica de LisboaLisboa chengdu KaitengSifang Digital Radio&TV Equipment Co. LtdChengduSichuan China academy of engineering physics MianyangSichuan China building materials academy Beijing China Center of Advanced Science and Technology Beijing China Institute of Atomic Energy Beijing China Jiliang university HangzhouZhejiang China Nuclear Instrument CO. LTDBeijing China university of geosciences Beijing China university of geosciences WuhanHubei China university of Mining and Technology XuzhouJiangsu China West Normal university NanchongSichuan Chongqing university Department of PhysicsChongqing Chulalongkorn university Bangkok City university of Hong Kong Department of PhysicsHong Kong Cockcroft Institute Daresbury Cornell university IthacaNY CPPM(AMU/IPhU&CNRS/INP) Marseille Dalian Minzu university DalianLiaoning Dalian university of Technology Department of PhysicsDalian Deutsches Elektronen-Synchrotron DESY Hamburg Dzhelepov Laboratory of Nuclear Problems of Joint Institute for Nuclear Research(DLNP JINR) Dubna East China university Of Science And Technol

The Circular Electron Positron Collider(CEPC)is a large scientific project initiated and hosted by China,fostered through extensive collaboration with international *** complex comprises four accelerators:a 30 GeV Linac,a 1.1 GeV Damping Ring,a Booster capable of achieving energies up to 180 GeV,and a Collider operating at varying energy modes(Z,W,H,and tt).The Linac and Damping Ring are situated on the surface,while the subterranean Booster and Collider are housed in a 100 km circumference underground tunnel,strategically accommodating future expansion with provisions for a potential Super Proton Proton Collider(SPPC).The CEPC primarily serves as a Higgs *** its baseline design with synchrotron radiation(SR)power of 30 MW per beam,it can achieve a luminosity of 5×10^(34)cm^(-2.s^(-1)per interaction point(IP),resulting in an integrated luminosity of 13 ab^(-1)for two IPs over a decade,producing 2.6 million Higgs *** the SR power to 50 MW per beam expands the CEPC's capability to generate 4.3 million Higgs bosons,facilitating precise measurements of Higgs coupling at sub-percent levels,exceeding the precision expected from the HL-LHC by an order of *** Technical Design Report(TDR)follows the Preliminary Conceptual Design Report(Pre-CDR,2.1.)and the Conceptual Design Report(CDR,2.1.),comprehensively detailing the machine's layout,performance metrics,physical design and analysis,technical systems design,R&D and prototyping efforts,and associated civil engineering ***,it includes a cost estimate and a preliminary construction timeline,establishing a framework for forthcoming engineering design phase and site selection *** is anticipated to begin around 2.2.-2.2.,pending government approval,with an estimated duration of 8 *** commencement of experiments and data collection could potentially be initiated in the mid-2.30s.

关键词： initiated exceeding precise

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An essential role of serum B-type natriuretic peptide in patients with acute inferior myocardial infarction

An essential role of serum B-type natriuretic peptide in pat...

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第十次中国中西医结合学会心血管病学术大会暨第五次江西省中西医结合学会心血管病学术大会

作者： Ling chen jianxin Hu Yanqi Wu {2. Huang {2.shu cheng~△Department of Cardiology,the Second Affiliated Hospital of Nanchang university, Nanchang 330006,JiangXi Province,China

Objective:To investigate the relationship between the level of serum B-type natriuretic peptide(BNP) and right ventricular infarction in patient s with acute inferior myocardial infarction(AIMI).Method:The serum BNP level was measured in 2.3 consecutive patient s with AIMI who were admitted in the coronary care unit(CCU) and in the normal control with 98 cases from October 2.06 to May *** patients were divided into five groups in accordance with the coronary angiographic findings and electrocardiogram(ECG):Acute inferior myocardial infarction group;acute inferior myocardial infarction and right ventricular infarction group;control group;left anterior circumflex(LXC) group;proximal,middle and distal segment of right coronary artery(RCA)*** patient s were performed directly percutaneous coronary intervention(PCI) within 2. hours after the onset of *** incidence of major adverse cardiac events(MACE),including arrhythmia, heart failure,cardiac shock and mortality,had been observed during hospital,30 days and 3 ***:BNP level in the acute inferior myocardial infarction and right ventricular infarction group was significantly higher than that in acute inferior myocardial infarction group(P101).The level in the proximal2.id segment of RCA group was higher than that in the LXC group(P101).Additionally,logistic regression analysis showed that the level of BNP was an independent predictor of MACE in the 30 days and 3 months in acute inferior myocardial infarction patient s (r=0.701 0,95%CI1 - 0.615,P101).Conclusion:We demonstrated the level of BNP in patients with acute inferior myocardial infarction or/ and right ventricular infarction,and BNP could be a good predictor for patients with acute inferior myocardial infarction and right ventricular infarction.

关键词： myocardial infarction natriuretic peptide

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基于iTRAQ蛋白组学的植物乳杆菌镉吸附及耐受特征分析

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中国食品学报 2019年第4期19卷 1-15页

作者：甘雨肖越翟齐啸赵建新张灏陈卫江南大学食品学院江苏无锡214122

植物乳杆菌是在食品工业中广泛应用的一种益生菌,不同植物乳杆菌对重金属镉的吸附及耐受能力存在显著的菌株差异。为了探究植物乳杆菌的镉吸附机制,通过筛选得到两株镉吸附能力有显著差别的植物乳杆菌(强吸附菌株CCFM8610和弱吸附菌株CC... 详细信息

植物乳杆菌是在食品工业中广泛应用的一种益生菌,不同植物乳杆菌对重金属镉的吸附及耐受能力存在显著的菌株差异。为了探究植物乳杆菌的镉吸附机制,通过筛选得到两株镉吸附能力有显著差别的植物乳杆菌(强吸附菌株CCFM8610和弱吸附菌株CCFM595),采用基于同位素标记相对和绝对定量(iTRAQ)方法的比较蛋白组学分析手段,研究两株菌间的差异表达蛋白。在两菌株中共鉴定得到1 690个蛋白,其中有109个丰度差异表达蛋白,以及89个仅在强镉吸附菌株CCFM8610检测到的蛋白。通过对这些差异蛋白进行功能注释、代谢通路重构以及互作网络解析,阐明植物乳杆菌CCFM8610与CCFM595的镉吸附及耐受机制可能为:表达更多的参与细胞壁及胞外聚合物合成的蛋白,从而加强镉离子的隔离和吸附;CCFM8610一直处于低压力的状态,表现为压力应答相关蛋白在CCFM8610中低表达;CCFM8610具有独特的能量节省的代谢模式,表现为TCA循环通路蛋白的整体较低水平;CCFM8610具有更强的金属外排转运能力,表现为cadA等镉外排泵也在CCFM8610具有更高丰度;CCFM8610的疏水性氨基酸的表达增加,可增强细胞的表面疏水性,还能通过表达调节渗透压及胞内结合镉的氨基酸来应对镉毒性。

关键词：镉吸附蛋白组植物乳杆菌

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谷氨酰胺转氨酶对高水分豌豆蛋白挤出物结构及消化特性的影响

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食品科学 2023年第5期44卷 1-8页

作者：刘潇秦鉴新李江华周景文陈坚江南大学未来食品科学中心江苏无锡214122

本实验以豌豆蛋白为原料,利用双螺杆挤出机制备挤出物,探究高水分挤出过程中添加不同比例的谷氨酰胺转氨酶(transglutaminase,TG)对挤出物结构及消化特性的影响。通过质构分析、组织化度分析和扫描电子显微镜观察等方法表征挤出物的宏... 详细信息

本实验以豌豆蛋白为原料,利用双螺杆挤出机制备挤出物,探究高水分挤出过程中添加不同比例的谷氨酰胺转氨酶(transglutaminase,TG)对挤出物结构及消化特性的影响。通过质构分析、组织化度分析和扫描电子显微镜观察等方法表征挤出物的宏观结构和微观结构。通过测定流变特性、蛋白质溶解度以及消化特性评价挤出物的结构性能及营养价值。结果表明,添加适量TG(0.2.%~1.00%)促进了豌豆蛋白分子间交联,形成致密的网络结构,提高了挤出物的消化率,促进游离氨基酸释放。然而添加过量TG(2.00%)则对挤出物纤维的形成以及消化特性起到抑制作用。TG添加量为1.00%时,制备的豌豆蛋白高水分挤出物组织化程度与营养价值较好。综上,高水分挤压-TG法联用加工工艺能够有效改善植物蛋白肉的结构及消化特性。

关键词：谷氨酰胺转氨酶豌豆蛋白挤出物结构消化特性

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维普期刊数据库

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