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磺胺间二甲氧嘧啶纳米抗体免疫磁珠的制备及其鉴定

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中国生物化学与分子生物学报 2024年第2期40卷 223-230页

作者：程小容赵琳王迪王雅婷杨雅匀古少鹏何金鑫山西农业大学动物医学学院预防兽医系公共卫生学实验室山西太谷030801 南江县农业农村局四川南江635600

基于纳米抗体(nanobody,Nb)和磁小体(bacterial magnetic particles,BMPs)的免疫磁珠在污染物分离分析中具有良好的应用前景,然而,不同长度柔性连接肽(linker)对免疫磁珠性能的影响尚未见相关报道。为了探究柔性连接肽长度对免疫磁珠的... 详细信息

基于纳米抗体(nanobody,Nb)和磁小体(bacterial magnetic particles,BMPs)的免疫磁珠在污染物分离分析中具有良好的应用前景,然而,不同长度柔性连接肽(linker)对免疫磁珠性能的影响尚未见相关报道。为了探究柔性连接肽长度对免疫磁珠的性能影响,本研究使用pET-28a作为载体,在磺胺间二甲氧嘧啶(sulfadimethoxine,SDM)Nb基因上融合了不同长度的柔性连接肽,分别为pET28a-SDM-Nb-(G4S)1-Cys和pET28a-SDM-Nb-(G4S)4-Cys,并使用大肠杆菌BL21(DE3)作为重组工程菌进行表达,最终获得Nb-(G4S)1-Cys和Nb-(G4S)4-Cys重组蛋白质。利用异源双功能试剂3-(2-吡啶二巯基)丙酸N-羟基琥珀酰亚胺酯(N-succinimidyl-3-(2-pyridyldithiol)propionate,SPDP),分别将重组蛋白质与BMPs进行偶联,构建了免疫磁珠。利用免疫印迹对偶联结果进行了初步鉴定,并对偶联条件进行了优化。同时,使用透射电镜和Zeta电位分析仪对免疫磁珠的水合粒径、Zeta电位和分散性进行了分析。研究结果表明,SPDP能有效地将Nb-(G4S)1-Cys和Nb-(G4S)4-Cys定向固定在BMPs表面。通过差值法计算发现,Nb-(G4S)1-Cys与BMPs的偶联效率高于Nb-(G4S)4-Cys与BMPs的偶联效率。进一步表征结果显示,BMP-(G4S)1-Nb的Zeta电位绝对值更高,水合粒径更小,并且具有较低的多分散性指数,说明其在水相体系中具有更强的胶体稳定性。综上所述,利用BMPs和Nb-(G4S)1-Cys构建的免疫磁珠性能优于BMPs和Nb-(G4S)4-Cys构建的免疫磁珠。这为今后选择合适长度的连接肽构建高效的免疫磁珠分离分析SDM提供了理论依据。

关键词：磺胺间二甲氧嘧啶磁小体连接肽纳米抗体免疫磁珠

Structural Properties of Ions and Polyelectrolytes in Aqueous Solutions under External Electric Fields:The Sign Effect

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Chinese Journal of Polymer Science 2024年第9期42卷 1341-1352,I0007页

作者： cheng-Jiang Lin Jun-Jun Wang Yuan Jiang Shu-Li Chen Hong-Fei Li Wen-Han Zhao Qing-rong Huang Chang-Ru rong xiao-Zheng Duan State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun130022China School of Applied Chemistry and Engineering University of Science and Technology of ChinaHefei230026China National Key Laboratory of Advanced Vehicle Integration and Control China FAW Group Co.Ltd.Changchun130013China State Key Laboratory of Molecular Engineering of Polymers Department of Macromolecular ScienceFudan UniversityShanghai200438China Department of Food Science Rutgers University65 Dudley RoadNew BrunswickNew Jersey08901USA

We utilize molecular dynamics simulations to investigate the microstructures of ions and polyelectrolytes in aqueous solutions under external electric *** focusing on the multi-body interactions between ionic components and H_(2)O molecules,as well as their responses to the external electric fields,we clarify several nontrivial molecular features of the ionic and polyelectrolyte solutions,such as the solvations of cations and anions,clustering of the ions,and dispersions/aggregations of polyelectrolyte chains,as well as the corresponding responses of H_(2)O molecules in these *** simulations illustrate the variations in structures of ionic solutions caused by reversing the charge sign of the ions,and elucidate the disparity in structures between anionic and cationic polyelectrolyte solutions in the presence of the external electric *** work clarifies the mechanism for the alternations in complex multi-body interactions in aqueous solutions caused by the application electric field,which can contribute to the fundamental understanding of the physical and chemical natures of ion-containing and charged polymeric systems.

关键词： Polyelectrolyte Ions Aqueous solutions External electric fields Asymmetric dipole

Disinfection Byproducts and Their Precursors in Drinking Water Sources:Origins,Influencing Factors,and Environmental Insights

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Engineering 2024年第5期36卷 36-50页

作者： rong xiao Yang Deng Zuxin Xu Wenhai Chu State Key Laboratory of Pollution Control and Resource Reuse College of Environmental Science and EngineeringTongji UniversityShanghai 200092China Ministry of Education Key Laboratory of the Yangtze River Water Environment Tongji UniversityShanghai 200092China Department of Earth and Environmental Studies Montclair State UniversityMontclairNJ 07043USA

Tracing the contamination origins in water sources and identifying the impacts of natural and human processes are essential for ecological safety and public ***,current analysis approaches are not ideal,as they tend to be laborious,time-consuming,or technically *** byproducts(DBPs)are a family of well-known secondary pollutants formed by the reactions of chemical disinfectants with DBP precursors during water disinfection *** DBP precursors have various origins(e.g.,natural,domestic,industrial,and agricultural sources),and since the formation of DBPs from different precursors in the presence of specific disinfectants is distinctive,we argue that DBPs and DBP precursors can serve as alternative indicators to assess the contamination in water sources and identify pollution *** providing a retrospective of the origins of DBPs and DBP precursors,as well as the specific formation patterns of DBPs from different precursors,this article presents an overview of the impacts of various natural and anthropogenic factors on DBPs and DBP precursors in drinking water *** practice,the DBPs(i.e.,their concentration and speciation)originally present in source water and the DBP precursors determined using DBP formation potential tests—in which water samples are dosed with a stoichiometric excess of specific disinfectants in order to maximize DBP formation under certain reaction conditions—can be considered as alternative *** jointly used with other water quality parameters(e.g.,dissolved organic carbon,dissolved organic nitrogen,fluorescence,and molecular weight distribution)and specific contaminants of emerging concern(e.g.,certain pharmaceuticals and personal care products),DBPs and DBP precursors in drinking water sources can provide a more comprehensive picture of water pollution for better managing water resources and ensuring human health.

关键词： Disinfection byproducts Disinfection byproduct precursors Drinking water sources Contamination indicator Natural factors Human factors

Reconstruction of biomimetic ionic channels within covalent organic frameworks for ultrafast and selective uranyl capture

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Science China Chemistry 2024年第10期67卷 3423-3431页

作者： cheng-rong Zhang xiao-Juan Chen cheng-Peng Niu cheng Meng Shun-Mo Yi Xin Liu Jia-Xin Qi Qiu-Xia Luo Ru-Ping Liang Jian-Ding Qiu State Key Laboratory of Nuclear Resources and Environment East China University of TechnologyNanchang 330013China College of Chemistry and Chemical Engineering Nanchang UniversityNanchang 330031China

The efficient extraction of uranium,as the primary component of nuclear energy,holds significant *** inspiration from the charge interaction observed in biological ion channels,we encapsulated negatively charged polystyrene sulfonate(PSS)or sodium polystyrene carboxylate(PVBA)into the nanochannels of amidoxime functionalized covalent organic framework(COF-AO)in-situ to alter the cavity environment of *** synthesized COF-AO-PSS and COF-AO-PVBA are used for ultra-fast and highly selective uranium *** negatively charged PSS/PVBA was confined in the COF-AO channel providing the driving force for uranium transport and blocking other ions,thus creating a highly selective“uranium highway”.Additionally,introducing sulfonate groups or carboxyl groups into COF-AO offers supplementary coordination environments and weak interactions with *** to charge-assisted migration and various interaction mechanisms,both COF-AO-PSS and COF-AO-PVBA exhibit faster adsorption kinetics and higher selectivity compared to COF-AO *** adsorption capacities are 3.8 times and 2.4 times that of COF-AO alone respectively which highlights the necessity for constructing biomimetic ion channels in uranium adsorption *** work presents a bionic adsorbent based on covalent organic frameworks(COFs)for the first time,overcoming environmental and equipment limitations associated with traditional photocatalysis and electrocatalysis methods for uranium capture,opening up new avenues for designing multifunctional materials that mimic biological systems.

关键词： covalent organic frameworks Uranium biomimetic ion-channels adsorption

Strengthening and control of second-phase particle precipitation in ferritic/austenitic/martensitic heat-resistant alloys:a review

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Journal of Iron and Steel Research International 2024年第1期31卷 3-23页

作者： Xue-cheng Peng Han-jie Guo Xin-fang Zhang Yi-wa Luo Ye Sun Jing Guo rong-guang Yang xiao-dan Zheng School of Metallurgical and Ecological Engineering University of Science and Technology Beijing(USTB)Beijing100083China Beijing Key Laboratory of Special Melting and Preparation of High-End Metal Materials University of Science and Technology Beijing(USTB)Beijing100083China Beijing Shougang Gitane New MATERIALS Co. Ltd.Beijing102206China

Heat-resistant alloys with excellent mechanical properties are widely used in various fields,and further improvement in their properties is essential to meet the requirements in new-generation advanced supercritical boilers,nuclear reactors,superheaters,and other new materials *** effectively enhance the comprehensive performance of heat-resistant alloys,second-phase particle strengthening has been widely studied,and in the face of different service envi-ronments of advanced heat-resistant steels,the selection of suitable second-phase particles is essential to maximize the performance of these *** this end,three major types of reinforcing phases in heat-resistant alloys such as carbides,rare earth oxides,and intermetallic compounds are summarized.A comparative analysis of the precipitation behavior of the reinforcing phases with different types as well as the risks and means of controlling their use in service,is *** parameters for the application of various types of second-phase particles in heat-resistant alloys are provided to support the design and preparation of new ultrahigh-performance heat-resistant alloys.

关键词： Heat-resistant alloy Second-phase particle Carbide Rare earth oxide Intermetallic compound Strengthening

Correlation between the charge radii difference in mirror partner nuclei and thesymmetry energy slope

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Chinese Physics C 2024年第8期48卷 177-184页

作者： xiao-rong Ma Shuai Sun rong An Li-Gang Cao School of Physics Ningxia UniversityYinchuan 750021China Key Laboratory of Beam Technology of Ministry of Education College of Nuclear Science and TechnologyBeijing Normal UniversityBeijing 100875China Key Laboratory of Beam Technology of Ministry of Education Institute of Radiation TechnologyBeijing Academy of Science and TechnologyBeijing 100875China

A correlation between the charge radii difference of mirror partner nucleiΔR_(ch) and the slope parameter L of symmetry energy has been built to ascertain the equation of state of isospin asymmetric nuclear *** this work,the influences of pairing correlations and isoscalar compression modulus on theΔRch are systematically investigated based on the Skyrme energy density functional *** calculated results suggest that the linear correlation betweenΔR_(ch) and L is decreased by the surface pairing *** slope parameter deduced from the difference of charge radii of mirror-pair nuclei ^(32)Ar-^(32)Si,^(36)Ca-^(36)S,^(38)Ca-^(38)Ar,and ^(54)Ni-^(54)Fe falls into the range of L=42.57−50.64 MeV,that is,the rather soft equation of state of asymmetric nuclear ***,the range of the slope parameter can also be influenced by the effective forces classified by various isoscalar incompressibility coefficients.

关键词： symmetry energy charge radius mirror-pair nuclei slope parameter

Biphase-to-monophase structure evolution of Na_(0.766+x)Li_(x)Ni_(0.33-x)Mn_(0.5)Fe_(0.1)Ti_(0.07)O_(2) toward ultradurable Na-ion batteries

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Carbon Energy 2024年第9期6卷 66-79页

作者： Mengting Liu Zhiwei cheng Xu Zhu Haojie Dong Tianran Yan Liang Zhang Lu Zheng Hu-rong Yao Xian-Zuo Wang Lianzheng Yu Bing xiao Yao xiao Peng-Fei Wang State Key Laboratory of Electrical Insulation and Power Equipment School of Electrical EngineeringCenter of Nanomaterials for Renewable EnergyXi'an Jiaotong UniversityXi'anShaanxiChina Jiangsu Key Laboratory of Advanced Negative Carbon Technologies Institute of Functional Nano&Soft Materials(FUNSOM)Soochow UniversitySuzhouJiangsuChina Fujian Provincial Key Laboratory of Quantum Manipulation and New Energy Materials College of Physics and EnergyFujian Normal UniversityFuzhouFujianChina Wenzhou Key Laboratory of Sodium-Ion Batteries Institute for Carbon NeutralizationWenzhou University Technology InnovationWenzhouZhejiangChina

Layered composite oxide materials with O3/P2 biphasic crystallographic structure typically demonstrate a combination of high capacities of the O3 phase and high operation voltages of the P2 ***,their practical applications are seriously obstructed by difficulties in thermodynamic phase regulation,complicated electrochemical phase transition,and unsatisfactory cycling ***,we propose an efficient structural evolution strategy from biphase to monophase of Na_(0.766+x)Li_(x)Ni_(0.33-x)Mn_(0.5)Fe_(0.1)Ti_(0.07)O_(2) through Li+*** role of Li+substitution not only simplifies the unfavorable phase transition by altering the local coordination of transition metal(TM)cations but also stabilizes the cathode–electrolyte interphase to prevent the degradation of TM cations during battery *** a result,the thermodynamically robust O_(3)-Na_(0.826)Li_(0.06)Ni_(0.27)Mn_(0.5)Fe_(0.1)Ti_(0.07)O_(2) cathode delivers a high capacity of 139.4 mAh g^(-1) at 0.1 C and shows prolonged cycling life at high rates,with capacity retention of 81.6%at 5 C over 500 *** work establishes a solid relationship between the thermodynamic structure evolution and electrochemistry of layered cathode materials,contributing to the development of long-life sodium-ion batteries.

关键词： electrochemistry Li+substitution Na-ion batteries O_(3)phase phase transition

Compound Product of Hypermatrices

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Journal of Systems Science & Complexity 2024年第1期37卷 169-183页

作者： cheng Daizhan MENG Min ZHANG xiao JI Zhengping Key Laboratory of Systems and Control Academy of Mathematics and Systems ScienceChinese Academy of SciencesBeijing 100190China Department of Control Science and Engineering School of Electronics and Information EngineeringTongji UniversityShanghai 201210China National Center for Mathematics and Interdisciplinary Sciences Chinese Academy of SciencesBeijing 100190China School of Mathematical Sciences University of Chinese Academy of SciencesBeijing 100149China.

The semi-tensor product(STP)of matrices is generalized to multidimensional arrays,called the compound product of *** product is first defined for three-dimensional hypermatrices with compatible orders and then extended to general *** different types of hyperdeterminants are introduced and certain properties are *** Lie groups and Lie algebras corresponding to the hypermatrix products are ***,these results are applied to dynamical systems.

关键词： Compound semi-tensor product hyperdeterminants hypereigenvalues and hypereigenvectors hypergeneral linear groups hypermatrices.

Creep-Resistant Covalent Adaptable Networks with Excellent SelfHealing and Reprocessing Performance via Phase-Locked Dynamic Covalent Benzopyrazole-Urea Bonds

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Chinese Journal of Polymer Science 2024年第10期42卷 1545-1556,I0013页

作者： Miao Xie xiao-rong Wang Zhan-Hua Wang He-Sheng Xia State Key Laboratory of Polymer Materials Engineering Polymer Research InstituteSichuan UniversityChengdu610065China School of Petrochemical Engineering Liaoning Petrochemical UniversityFushun113001China

Covalent adaptive networks(CANs)are capable of undergoing segment rearrangement after being heated,which endows the materials with excellent self-healing and reprocessing performance,providing an efficient solution to the environment pollution caused by the plastic *** main challenge remains in developing CANs with both excellent reprocessing performance and creep-resistance *** this study,a series of CANs containing dynamic covalent benzopyrazole-urea bonds were developed based on the addition reaction between benzopyrazole and isocyanate *** calculation confirmed that relatively low dissociation energy is obtained through undergoing a five-member ring transition state,confirming excellent dynamic property of the benzopyrazole-urea *** verified by the FTIR results,this nice dynamic property can be well maintained after incorporating the benzopyrazole-urea bonds into polymer *** self-healing and reprocessing performance is observed by the 3-ABP/PDMS elastomers owing to the dynamic benzopyrazole-urea *** separation induced by the aggregation of the hard segments locked the benzopyrazole-urea bonds,which also makes the elastomers display excellent creep-resistance *** hard phase locking strategy provides an efficient approach to design CANs materials with both excellent reprocessing and creep-resistance performance.

关键词： Covalent adaptive networks Self-healing polymer Dynamic covalent bond