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突变芳香基硫酸酯酶H260L工程菌的发酵条件优化

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集美大学学报（自然科学版） 2017年第3期22卷 26-32页

作者：乔超超郭玉淅倪辉肖安风朱艳冰集美大学食品与生物工程学院福建厦门361021 福建省食品微生物与酶工程重点实验室福建厦门361021 厦门市食品与生物工程技术研究中心福建厦门361021 厦门南方海洋研究中心经济海藻资源化利用与深加工重点实验室福建厦门361021

通过摇瓶培养对突变芳香基硫酸酯酶H260L工程菌的发酵条件进行初步优化,研究不同发酵条件包括接种量、诱导时期、诱导剂浓度、发酵时间、诱导剂加入方式、发酵温度及培养基初始pH值对重组芳香基硫酸酯酶表达的影响。结果表明,重组芳香... 详细信息

通过摇瓶培养对突变芳香基硫酸酯酶H260L工程菌的发酵条件进行初步优化,研究不同发酵条件包括接种量、诱导时期、诱导剂浓度、发酵时间、诱导剂加入方式、发酵温度及培养基初始pH值对重组芳香基硫酸酯酶表达的影响。结果表明,重组芳香基硫酸酯酶工程菌发酵的乳糖诱导表达优化条件为:以5%接种量培养3 h后,加入乳糖诱导剂至5 g/L,诱导表达7 h;当发酵温度为25℃、培养基的初始pH值为7.5时,重组芳香基硫酸酯酶活性最高。在优化条件下,工程菌芳香基硫酸酯酶活性达到2.63 U/m L,是未优化酶活性的46.9倍。突变酶H260L对龙须菜粗多糖硫酸基团的脱硫率为82.1%。

关键词：突变芳香基硫酸酯酶发酵乳糖诱导表达

响应面法优化纤维素酶辅助从泡叶藻中提取泡叶藻聚糖的工艺

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过程工程学报 2018年第3期18卷 632-638页

作者：鲍青云韦敬柳乙姜泽东余刚黄高凌朱艳冰肖安风倪辉李清彪集美大学食品与生物工程学院福建厦门361021 福建省食品微生物与酶工程重点实验室福建厦门361021 厦门南方海洋研究中心海藻资源化利用与深加工重点实验室福建厦门361021 厦门市食品与生物工程技术研究中心福建厦门361021

通过单因素实验研究纤维素酶辅助提取泡叶藻聚糖时液料比、酶添加量、酶解温度、酶解时间等关键因素对泡叶藻聚糖提取率的影响,并进一步采用Box-Behnken实验设计和响应面分析法优化其工艺参数.结果表明,纤维素酶辅助提取泡叶藻聚糖的优... 详细信息

通过单因素实验研究纤维素酶辅助提取泡叶藻聚糖时液料比、酶添加量、酶解温度、酶解时间等关键因素对泡叶藻聚糖提取率的影响,并进一步采用Box-Behnken实验设计和响应面分析法优化其工艺参数.结果表明,纤维素酶辅助提取泡叶藻聚糖的优化工艺条件为液料比30 mL/g、酶浓度200 IU/mL、酶解时间2.0 h、酶解温度50℃,该条件下多糖提取率为14.65%±0.73%,与模型预测值14.75%非常接近,采用响应面法对泡叶藻聚糖提取条件进行优化合理可行.

关键词：纤维素酶辅助提取泡叶藻聚糖工艺优化响应面法

利用淀粉糖作碳源培养法夫酵母生产虾青素的研究

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中国食品学报 2009年第1期9卷 29-34页

作者：倪辉肖安风李利君吴光斌翁惠芬何国庆蔡慧农苏文金集美大学生物工程学院厦门361021 厦门市食品工程中心厦门361021 浙江大学生物系统工程与食品学院杭州310029

为选择一种适合法夫酵母发酵虾青素的工业碳源,分别以麦芽糖浆、果葡糖浆、标准糖浆3种淀粉糖为碳源做发酵试验。试验结果表明,以麦芽糖浆、果葡糖浆和标准糖浆为碳源摇瓶培养法夫酵母,虾青素的体积产率及细胞产率间没有显著差异;分别以... 详细信息

为选择一种适合法夫酵母发酵虾青素的工业碳源,分别以麦芽糖浆、果葡糖浆、标准糖浆3种淀粉糖为碳源做发酵试验。试验结果表明,以麦芽糖浆、果葡糖浆和标准糖浆为碳源摇瓶培养法夫酵母,虾青素的体积产率及细胞产率间没有显著差异;分别以3种淀粉糖为碳源,在20L机械搅拌罐中补料分批培养法夫酵母的发酵规律相似,但以标准糖浆为碳源的虾青素细胞产率和虾青素得率高于其它2种淀粉糖,分别达到0.38mg虾青素/g干酵母和0.25mg虾青素/g葡萄糖;以标准糖浆为碳源,在75L机械搅拌罐中补料分批培养法夫酵母的发酵规律与20L罐的发酵规律一致,但虾青素的细胞产率为0.33mg/g干菌体,略低于20L罐的细胞产率;虾青素的得率为0.34mg/L,略高于20L罐的虾青素得率。

关键词：法夫酵母虾青素淀粉糖碳源工业发酵

Observation of acoustic hybrid topological phases induced by the p-d orbital interactions

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 132-139页

作者： xiao Xiang Feng Gao Yu-Gui Peng Peng Wu Zong-Lin Li Xue-Feng Zhu School of Physics and Innovation Institute Huazhong University of Science and Technology

In spinless systems,growing attention has recently been attracted to synthetic gauge fields,which redefine the fundamental crystal symmetries by utilizing the projective algebraic ***,synthetic gauge fields have been commonly investigated in single orbital systems,and explorations on hybrid orbitals remain elusive in ***,we report the experimental observation of hybrid topological phases induced by hybrid synthetic gauge flux,which is formed by the interaction between p and d orbitals embedded in acoustic *** breaking the translation symmetries of Lxand/or Ly,we unambiguously demonstrate the first-order M?bius edge states and higher-order corner *** work establishes a platform for seeking exotic topological phases induced by hybrid-orbitals interactions and initializing the framework of hybrid-orbitalsrelated topological *** applications can be anticipated in scenarios such as high-quality sensing and robust wave trapping due to the stepwise energy distribution of the hybrid topological phase.

关键词： topological insulator synthetic gauge field projective symmetry corner states

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米曲霉多蛋白酶代谢动力学初步研究

中国食品学报 2009年第1期9卷 46-51页

作者：杨远帆倪辉胡阳黄高凌肖安风李利君黄志勇蔡慧农集美大学生物工程学院厦门361021

目的:利用米曲霉能分泌多种蛋白酶的特性,研究该菌培养液水解鱼粉的动力学。方法:采用Folin法测定不同时间培养液的蛋白酶活力,甲醛滴定法测定不同时间培养液的氨基态氮,SDS-PAGE凝胶电泳测定水解产物的大小及培养液中产物的相对分子质... 详细信息

目的:利用米曲霉能分泌多种蛋白酶的特性,研究该菌培养液水解鱼粉的动力学。方法:采用Folin法测定不同时间培养液的蛋白酶活力,甲醛滴定法测定不同时间培养液的氨基态氮,SDS-PAGE凝胶电泳测定水解产物的大小及培养液中产物的相对分子质量。结果:米曲霉在以鱼蛋白为唯一氮源的培养基中能够产生多种蛋白酶,且随时间和底物的不同,蛋白酶的活力与种类都不同。结论:米曲霉是一种产多种蛋白酶的菌种。

关键词：米曲霉蛋白酶动力学鱼蛋白

贮藏条件对江蓠琼胶酶活性和琼胶凝胶强度的影响

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水产科学 2020年第6期39卷 880-886页

作者：张曦文伍菱童风景姜泽东朱艳冰肖安风倪辉李清彪集美大学食品与生物工程学院福建厦门361021 福建省食品微生物与酶工程重点实验室福建厦门361021 厦门南方海洋研究中心经济海藻资源化利用重点实验室福建厦门361021 厦门市食品生物工程技术研究中心福建厦门361021

通过调控保鲜盒中湿度获得水分为10%、14%、40%、60%的江蓠样品,然后将各组江蓠样品密封保鲜盒分别置于5、15、25℃和35℃恒温培养箱中,模拟贮藏温度和湿度环境,连续处理4 d,每日测定处理后江蓠中琼胶酶活性、还原糖含量、琼胶提取得率... 详细信息

通过调控保鲜盒中湿度获得水分为10%、14%、40%、60%的江蓠样品,然后将各组江蓠样品密封保鲜盒分别置于5、15、25℃和35℃恒温培养箱中,模拟贮藏温度和湿度环境,连续处理4 d,每日测定处理后江蓠中琼胶酶活性、还原糖含量、琼胶提取得率、琼胶凝胶强度等指标,连续测定4 d、每组3次。试验结果表明,环境温度和湿度的变化对江蓠琼胶酶活性和所提取的琼胶凝胶强度具有重要的影响,而湿度是最关键的影响因素,江蓠琼胶酶活性和琼胶的凝胶强度随着湿度的上升呈升高趋势。在贮藏开始1 d和2 d后,在江蓠水分含量为10%时提取琼胶的凝胶强度普遍高于水分含量为60%的江蓠所提取琼胶的凝胶强度,其胶凝胶强度最高达到604.2±136.7。

关键词：江蓠琼胶还原糖琼胶酶活性凝胶强度

miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow-derived neural crest cells

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Neural Regeneration Research 2025年第1期20卷 277-290页

作者： Meng Cong Jing-Jing Hu Yan Yu xiao-Li Li xiao-Ting Sun Li-Ting Wang Xia Wu Ling-Jie Zhu xiao-Jia Yang Qian-Ru He Fei Ding Hai-Yan Shi School of Medicine Nantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Coinnovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Physiology Jiangsu Health Vocational CollegeNanjingJiangsu ProvinceChina Department of Pathology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

Our previous study found that rat bone marrow–derived neural crest cells(acting as Schwann cell progenitors)have the potential to promote long-distance nerve ***-based therapy can enhance peripheral nerve repair and regeneration through paracrine bioactive factors and intercellular ***,the complex contributions of various types of soluble cytokines and extracellular vesicle cargos to the secretome remain *** investigate the role of the secretome and extracellular vesicles in repairing damaged peripheral nerves,we collected conditioned culture medium from hypoxia-pretreated neural crest cells,and found that it significantly promoted the repair of sensory neurons damaged by oxygen-glucose *** mRNA expression of trophic factors was highly expressed in hypoxia-pretreated neural crest *** performed RNA sequencing and bioinformatics analysis and found that miR-21-5p was enriched in hypoxia-pretreated extracellular vesicles of neural crest ***,to further clarify the role of hypoxia-pretreated neural crest cell extracellular vesicles rich in miR-21-5p in axonal growth and regeneration of sensory neurons,we used a microfluidic axonal dissociation model of sensory neurons in vitro,and found that hypoxia-pretreated neural crest cell extracellular vesicles promoted axonal growth and regeneration of sensory neurons,which was greatly dependent on loaded ***,we constructed a miR-21-5p-loaded neural conduit to repair the sciatic nerve defect in rats and found that the motor and sensory functions of injured rat hind limb,as well as muscle tissue morphology of the hind limbs,were obviously *** findings suggest that hypoxia-pretreated neural crest extracellular vesicles are natural nanoparticles rich in ***-21-5p is one of the main contributors to promoting nerve regeneration by the neural crest cell *** helps to explain the mechanism of action of the secretome and e

关键词： axotomy cell-free therapy conditioned medium extracellular vesicles hypoxic preconditioning microRNA oxygen-glucose deprivation peripheral nerve injury Schwann cell precursors

Endoplasmic reticulum stress and autophagy in cerebral ischemia/reperfusion injury:PERK as a potential target for intervention

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Neural Regeneration Research 2025年第5期20卷 1455-1466页

作者： Ju Zheng Yixin Li Ting Zhang Yanlin Fu Peiyan Long xiao Gao Zhengwei Wang Zhizhong Guan xiaolan Qi Wei Hong Yan xiao Key Laboratory of Endemic and Ethnic Diseases Ministry of Education&Key Laboratory of Medical Molecular Biology of Guizhou ProvinceGuizhou Medical UniversityGuiyangGuizhou ProvinceChina Guizhou Center for Disease Control and Prevention GuiyangGuizhou ProvinceChina Department of Histology and Embryology Guizhou Medical UniversityGuiyangGuizhou ProvinceChina Collaborative Innovation Center for Prevention and Control of Endemic and Ethnic Regional Diseases Co-constructed by the Province and Ministry GuiyangGuizhou ProvinceChina

Several studies have shown that activation of unfolded protein response and endoplasmic reticulum(ER)stress plays a crucial role in severe cerebral ischemia/reperfusion *** occurs within hours after cerebral ischemia,but the relationship between ER stress and autophagy remains *** this study,we established experimental models using oxygen-glucose deprivation/reoxygenation in PC12 cells and primary neurons to simulate cerebral ischemia/reperfusion *** found that prolongation of oxygen-glucose deprivation activated the ER stress pathway protein kinase-like endoplasmic reticulum kinase(PERK)/eukaryotic translation initiation factor 2 subunit alpha(e IF2α)-activating transcription factor 4(ATF4)-C/EBP homologous protein(CHOP),increased neuronal apoptosis,and induced ***,inhibition of ER stress using inhibitors or by si RNA knockdown of the PERK gene significantly attenuated excessive autophagy and neuronal apoptosis,indicating an interaction between autophagy and ER stress and suggesting PERK as an essential target for regulating *** autophagy with chloroquine exacerbated ER stress-induced apoptosis,indicating that normal levels of autophagy play a protective role in neuronal injury following cerebral ischemia/reperfusion *** from this study indicate that cerebral ischemia/reperfusion injury can trigger neuronal ER stress and promote autophagy,and suggest that PERK is a possible target for inhibiting excessive autophagy in cerebral ischemia/reperfusion injury.

关键词： apoptosis ATF4 autophagy C/EBP homologous protein cerebral ischemia/reperfusion injury eIF2α endoplasmic reticulum stress PERK

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乙醇补料对法夫酵母发酵产虾青素的促进效果

集美大学学报（自然科学版） 2017年第4期22卷 20-27页

作者：付爽沈宁燕倪辉李利君杨秋明肖安风集美大学食品与生物工程学院福建厦门361021 福建省食品微生物与酶工程重点实验室福建厦门361021 福建省海洋功能食品工程技术研究中心福建厦门361021 厦门市海洋功能食品重点实验室福建厦门361021

将法夫酵母(Phaffia rhodozyma)JMU-MVP14菌株在7 L发酵罐中进行培养,考察在不同葡萄糖质量浓度条件下,补加乙醇对法夫酵母JMU-MVP14菌株的生长和虾青素合成的影响。结果表明:低糖质量浓度下(20 g/L),恒定乙醇浓度发酵明显提高虾青素产... 详细信息

将法夫酵母(Phaffia rhodozyma)JMU-MVP14菌株在7 L发酵罐中进行培养,考察在不同葡萄糖质量浓度条件下,补加乙醇对法夫酵母JMU-MVP14菌株的生长和虾青素合成的影响。结果表明:低糖质量浓度下(20 g/L),恒定乙醇浓度发酵明显提高虾青素产量和细胞产率,与分批发酵相比,虾青素产量和细胞产率分别提高了27%和40%,分别达到102.8 mg/L和5.9 mg/g;中糖质量浓度下(30 g/L),恒定乙醇浓度发酵,得到较高的虾青素产量为118.4 mg/L,与分批发酵相比提高了34%;高糖质量浓度下(60 g/L),发酵得到较高虾青素产量为280.6 mg/L,是低糖质量浓度培养下虾青素产量的3.5倍,恒定乙醇发酵与分批发酵相比,生物量和虾青素产量均有显著降低。

关键词：法夫酵母虾青素乙醇补料