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Single cell analysis reveals inhibition of angiogenesis attenuates the progression of heterotopic ossification in Mkx^(−/−)mice

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Bone Research 2022年第4期10卷 715-725页

作者： Junxin Lin yuwei Yang Wenyan Zhou Chao Dai xiao Chen Yuanhao Xie Shan Han Huanhuan Liu Yejun Hu Chenqi Tang Varitsara Bunpetch Dandan Zhang Yishan Chen xiaohui Zou Di Chen Wanlu Liu Hongwei Ouyang Dr.Li Dak Sum&Yip Yio Chin Center for Stem Cells and Regenerative Medicine and Department of Orthopedic Surgery of the Second Affiliated HospitalZhejiang University School of MedicineHangzhouChina Department of Sports Medicine Zhejiang University School of MedicineHangzhouChina Zhejiang University-University of Edinburgh Institute Zhejiang University School of Medicineand Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang ProvinceHangzhouChina China Orthopedic Regenerative Medicine Group(CORMed) HangzhouChina Department of Pathology Zhejiang University School of MedicineHangzhouChina Clinical Research Center The First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhouChina Center for Reproductive Medicine The Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhouChina

Tendon heterotopic ossification(HO)is characterized by bone formation inside tendon tissue,which severely debilitates people in their daily *** therapies fail to promote functional tissue repair largely due to our limited understanding of HO ***,we investigate the pathological mechanism and propose a potential treatment method for *** assays showed that the Mohawk(MKX)expression level was decreased in human tendon HO tissue,coinciding with spontaneous HO and the upregulated expression of osteochondrogenic and angiogenic genes in the tendons of Mkx^(−/−)***-cell RNA sequencing analyses of wild-type and Mkx^(−/−)tendons identified three cell types and revealed the excessive activation of osteochondrogenic genes during the tenogenesis of Mkx^(−/−)tendon ***-cell analysis revealed that the gene expression program of angiogenesis,which is strongly associated with bone formation,was activated in all cell types during ***,inhibition of angiogenesis by the small-molecule inhibitor BIBF1120 attenuated bone formation and angiogenesis in the Achilles tendons of both Mkx mutant mice and a rat traumatic model of *** findings provide new insights into the cellular mechanisms of tendon HO and highlight the inhibition of angiogenesis with BIBF1120 as a potential treatment strategy for HO.

关键词： angiogenesis ossification analysis

Box-Behnken响应面法优化竹沥半夏炮制工艺

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时珍国医国药 2024年第2期35卷 352-355页

作者：罗美美朱伟豪郑琳玉朱星宇梅春梅陈富贵赵雨薇郝芳雨胡晓芳李伟东南京中医药大学药学院江苏南京210023 江苏省中药炮制重点实验室江苏南京210023

目的优化竹沥半夏炮制工艺,为竹沥半夏饮片临方炮制规范化研究提供参考。方法采用Box-Behnken响应面法设计分析,以浸出物、次黄嘌呤、尿苷、腺嘌呤、鸟苷的综合评分为评价指标,考察竹沥半夏辅料用量、炮制时间、干燥温度等3个因素,对竹... 详细信息

目的优化竹沥半夏炮制工艺,为竹沥半夏饮片临方炮制规范化研究提供参考。方法采用Box-Behnken响应面法设计分析,以浸出物、次黄嘌呤、尿苷、腺嘌呤、鸟苷的综合评分为评价指标,考察竹沥半夏辅料用量、炮制时间、干燥温度等3个因素,对竹沥半夏炮制工艺进行优选,同时对最佳工艺进行验证。结果竹沥半夏最佳炮制工艺为:每100 g姜半夏,鲜竹沥15 g,炮制时间1 h,干燥温度44℃。结论该工艺简易可行、竹沥半夏质量稳定可靠,为竹沥半夏的临床应用提供了科学依据。

关键词：竹沥半夏 Box-Behnken响应面法炮制工艺总评归一值

Highlight stress enhanced the disturbances of the rhythmic expression of Kai genes caused by the knockout of prx-2cys in Synechococcus elongatus PCC 7942

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Highlight stress enhanced the disturbances of the rhythmic e...

Anaerobic digestion of sludge by different pretreatments:Changes of amino acids and microbial community

2016年全国植物生物学大会

作者： Jing Cai xiao Luo Pengfei Li Pan Lai yuwei Zhao Life sciences school of Northwest University key laboratory of resources biology and biotechnology in Western ChinaMinistry of education

The central oscillator of circadian clock in cyanobacterium,Synechococcus elongatus PCC 7942,is composed of kai A,kai B,kai C proteins. Many researches confirmed that this self-sustained intracellular circadian clock,so-called Kai clock,have played a pivotal role in the generation and calibration of the time clue for the circadian rhythm in cyanobacterial metabolism. Some proteins,such as Sas A、Lab A、Cik A、Rap A,are considered to be the key switches that have operated the output pathways of Kai clock. Peroxiredoxins(Prxs) in short,are families of thiol-specific antioxidant protein,which have be convinced to exert an important function in the whole antioxidant defense system of cyanobacteria,the rhythmic changes redox state of 2Cys-Prx are proved as a conserved non-transcriptional rhythmic marker. To test the relationship between the Kai clock and the PRX-SO rhythmic marker,the expression patterns of clock genes were detected of by q PCR method in Wild-type(WT) and prx-2cys gene knockout(Δprx-2cys) trains under different light intensity. The results demonstrated that under the natural light condition(100μEms),the rhythmic expression phenotype of the central clock genes were not affected by whether the prx-2cys gene were knocked out in *** PCC 7942 cells,while the expression patterns of the genes operating the output pathway were slightly affected. Interestingly,under a high light stressed condition(400μEms),the expression patterns of the output pathway genes were severely affected both in WT and Δprx-2cys strains. The circadian rhythmic expression pattern of the central clock genes nearly disappeared in WT strains,while more robust rhythmic expression profiles of sas A 、 lab A 、 cik A 、 rap A were restored inΔprx-2cys cells by an unknown method.

关键词： cyanobacterium peroxiredoxin gene knockout antioxidant circadian rhythm

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Frontiers of Environmental Science & Engineering 2022年第2期16卷 109-123页

作者： Keke xiao Zecong Yu Kangyue Pei Mei Sun yuwei Zhu Sha Liang Huijie Hou Bingchuan Liu Jingping Hu Jiakuan Yang School of Environmental Science and Engineering Huazhong University of Science and TechnologyWuhan 430074China Hubei Provincial Engineering Laboratory of Solid Waste Treatment Disposal and Recycle TechnologyWuhan 430074China State Key Laboratory of Coal Combustion Huazhong University of Science and TechnologyWuhan 430074China

Many studies have investigated the effects of different pretreatments on the performance of anaerobic digestion of ***,the detailed changes of dissolved organic nitrogen,particularly the release behavior of proteins and the byproducts of protein hydrolysis-amino acids,are rarely known during anaerobic digestion of sludge by different *** we quantified the changes of three types of proteins and 17 types of amino acids in sludge samples solubilized by ultrasonic,thermal,and acid/alkaline pretreatments and their transformation during anaerobic digestion of *** protein,aromatic protein I,aromatic protein II,and cysteine were identified as the key dissolved organic nitrogen responsible for methane production during anaerobic digestion of sludge,regardless of the different pretreatment *** from the depletion of other amino acids,cysteine was resistant to degradation after an incubation period of 30 days in all sludge ***,the“cysteine and methionine metabolism(K00270)”was absent in all sludge samples by identifying 6755 Kyoto Encyclopedia of Genes and Genomes assignments of genes *** contributed to the generation of methane and the degradation of acetic,propionic,and n-butyric acids through decreasing oxidation-reduction potential and enhancing biomass *** study provided an alternative strategy to enhance anaerobic digestion of sludge through in situ production of cysteine.

关键词： Sludge pretreatments Dissolved organic nitrogen Proteins Amino acids Structural equation model Metagenomic sequencing analysis

“十三五”中国海油固井技术研究进展与发展建议

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中国海上油气 2020年第5期32卷 145-151页

作者：罗宇维肖伟赵军中海油田服务股份有限公司河北三河065201

"十三五"期间,针对中国海油固井技术从高温高压探井到高温高压气田开发井,从深水到超深水,从油气井水泥环短期防窜到全生命周期完整性等跨越面临的技术挑战,经过系统研究攻关,形成了高温高压井固井技术、深水井固井技术、非常规井固井... 详细信息

"十三五"期间,针对中国海油固井技术从高温高压探井到高温高压气田开发井,从深水到超深水,从油气井水泥环短期防窜到全生命周期完整性等跨越面临的技术挑战,经过系统研究攻关,形成了高温高压井固井技术、深水井固井技术、非常规井固井技术、复杂盐膏层固井技术等具中国海油特色的固井技术体系。未来,为了适应中国海油持续增加的勘探开发力度,固井技术面临超高温高压、复合酸性气体腐蚀和超窄压力窗口挑战,超深水复杂浅层地质灾害、深水复杂开发井井筒完整性挑战,超低成本、超高时效挑战。建议中国海油固井技术发展需加强海上超高温高压复杂地层安全固井技术、超深水复杂地层安全固井技术、正常温压井降本增效的固井技术等方面研究,为中国海油油气勘探开发提供强有力的技术支撑。

关键词：中国海油固井技术高温高压超深水技术挑战发展建议

Activated carbon fiber mediates efficient activation of peroxymonosulfate systems:Modulation of manganese oxides and cycling of manganese species

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Chinese Chemical Letters 2023年第12期34卷 275-280页

作者： Lixi xiao yuwei Deng Huo Zhou Fengjun Lu Chong Ke Yuxuan Ye Xuanyuan Pei Dongsheng Xia Fei Pan School of Environmental Engineering Wuhan Textile UniversityWuhan 430200China State Key Laboratory of New Textile Materials and Advanced Processing Technologies Wuhan Textile UniversityWuhan 430200China

Manganese oxides show a strong catalytic activity in the peroxymonosulfate(PMS)advanced oxidation process but have poor chemical stability and a propensity to cause the aggregation of ***,a novel composite material(abbreviated as MnO_(x)@ACF)was synthesized,characterized,and *** carbon fiber(ACF)was selected as a carrier,which modulated the composition of manganese *** results showed that MnO_(x)@ACF had a strong adsorption ability and successfully activated PMS to degrade tetracycline hydrochloride(TCH),with a removal eficiency of 89.0%in 30 *** factors such as pH and coexisting ion species were investigated,and a five-cycle test was *** oxygen(^(1)O_(2))was predominated in the MnO_(x)@ACF/PMS system.A possible explanatory pathway of TCH was proposed based on the results of the high performance liquid chromatography-mass *** was concluded that this study provides a novel insight into the activation of PMS for the degradation of organic matter by carbon-loaded multivalent manganese oxides.

关键词： Activated carbon fiber Mn_(3)O_(4) MnO_(2) Singlet oxygen

The rhythmic expression of clock genes and stress responding genes of Tibetan hulless barley under low temperature

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The rhythmic expression of clock genes and stress responding...

Systemic and single cell level responses to 1 nm size biomaterials demonstrate distinct biological effects revealed by multi-omics atlas

2016年全国植物生物学大会

作者： Pengfei Li Jing Cai Tianliang Chang xiao Luo Pan Lai yuwei Zhao Life sciences school of Northwest University key laboratory of resources biology and biotechnology in Western ChinaMinistry of education

Tibetan hulless barley(Hordeum vulgare L. var. nudum. hook. f.) has been cultured as main cereal crops by the inhabitants on Tibetan plateau for thousands of year. Compared to the normal cultivated barley and all other plants within the genus Hordeum,the hulless barley has developed stronger endogenous resistance systems to survive and grow under the general environmental stresses in the Tibetan Plateau. However,little of the mechanism of these stress defense systems has been reported. The low temperature acclimation process but non-freezing temperatures would trigger rapid signaling events in target plants which often leads to the responding expression of cold-regulated genes,as well as their transcription factors,for an anticipation of the coming environmental freezing tolerance. In order to find out whether the circadian rhythmic expression profiles of clock genes in hulless barley,such as TOC1,CCA1,LHY,etc. and the cold responding genes,either CBF,LEA in ABA pathway or NHX,CBL and CIPK in non-ABA pathway are affected by the non-freezing low temperature stress,q PCR method was employed to detect the rhythmic expression patterns of those above-mentioned genes in time course hulless barley seedlings which had been stressed by non-freezing low temperatures under LL condition. The primary results demonstrated that in the control group(under 25°C),the expression profiles of the circadian clock genes showed robust circadian rhythmic,as well as which of those non-ABA pathway cold-stress responding genes displayed obvious circadian rhythmic profiles,while none of the stress responding genes′ expression involved in the ABA pathway were proved to be clock controlled. Under the low temperature(4°C) treated condition,the rhythmic expression patterns of most clock genes maintained their robust rhythmic though that of GI and LUX showed slight phase shifting and dimmed amplitudes. In terms of the stress responding genes in nonABA pathway,their expression profiles were severely

关键词： hulless barley circadian clock stress responding genes low temperature signal

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Bioactive Materials 2022年第12期7卷 199-212页

作者： Tao Zhang Tingyun Lei Ruojin Yan Bo Zhou Chunmei Fan Yanyan Zhao Shasha Yao Haihua Pan Yangwu Chen Bingbing Wu yuwei Yang Lijuan Hu Shen Gu xiaoyi Chen Fangyuan Bao Yu Li Hanqi Xie Ruikang Tang xiao Chen Zi Yin Dr.Li Dak Sum&Yip Yio Chin Center for Stem Cells and Regenerative Medicine and Department of Orthopedic Surgery of Sir Run Shaw HospitalZhejiang University School of MedicineHangzhou310058PR China Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province School of MedicineZhejiang UniversityHangzhou310058PR China China Orthopedic Regenerative Medicine Group CORMed Hangzhou310058PR China Department of Orthopaedic Surgery of Sir Run Run Shaw Hospital Zhejiang University School of MedicineHangzhou310058PR China Qiushi Academy for Advanced Studies Zhejiang UniversityHangzhou310058PR China Department of Pathology of the First Affiliated Hospital Anhui University of Chinese MedicineHefei230031PR China chool of Biomedical Sciences The Chinese University of Hong KongShatinN.THKSARChina

Although ultra-small nanoclusters(USNCs,<2 nm)have immense application capabilities in biomedicine,the investigation on body-wide organ responses towards USNCs is ***,applying a novel strategy of single-cell mass cytometry combined with Nano Genome Atlas of multi-tissues,we systematically evaluate the interactions between the host and calcium phosphate(CaP)USNCs at the organism *** single-cell mass cytometry,and magnetic luminex assay results,we identify dynamic immune responses to CaP USNCs at the single cell *** innate immune is initially activated and followed by adaptive immune activation,as evidenced by dynamic immune cells ***,using Nano Genome Atlas of multi-tissues,we uncover CaP USNCs induce stronger activation of the immune responses in the cartilage and subchondral bone among the five local tissues while promote metabolic activities in the liver and ***,based on the immunological response profiles,histological evaluation of major organs and local tissue,and a body-wide transcriptomics,we demonstrate that CaP USNCs are not more hazardous than the Food and Drug Administration-approved CaP nanoparticles after 14 days of *** findings provide valuable information on the future clinical applications of USNCs and introduce an innovative strategy to decipher the whole body response to implants.

关键词： Nanoparticle Nanocluster Single-cell mass cytometry RNA-Seq Multi-omics

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具身记忆及其内在机制

心理科学进展 2022年第11期30卷 2497-2506页

作者：金雨薇孙潇宋耀武河北大学教育学院保定071002

具身记忆研究主要探讨身体及其感觉运动过程在记忆中的作用。操纵或限制身体的物理状态、具身特征或可用性,可以影响记忆的效率、效价和内容。其内在机制可以通过编码特异性原理、启动效应、隐喻表征理论和再激活假说进行解释,但尚未形... 详细信息

具身记忆研究主要探讨身体及其感觉运动过程在记忆中的作用。操纵或限制身体的物理状态、具身特征或可用性,可以影响记忆的效率、效价和内容。其内在机制可以通过编码特异性原理、启动效应、隐喻表征理论和再激活假说进行解释,但尚未形成统一的模型。基于现有研究与理论的共性,提出具身记忆的感觉运动模拟模型,强调重新激活在具身记忆中的作用,以期在同一框架下说明身体作用于记忆的过程,并进一步指出重新激活的条件。未来亟需在具身记忆的稳定性以及深层机制等方面进行多维度的基础和应用研究。

关键词：记忆身体具身认知感觉运动模拟模型编码特异性原理