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Expression profiles of penaeidin from Fenneropenaeus chinensis in response to WSSV and vibrio infection by real-time PCR

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Acta Oceanologica Sinica 2005年第2期24卷 131-140页

作者： DONGBo LIUFengsong xiangjianhai LIFuhua GAOHongwei ExperimentalMarineBiologylaboratory InstituteofOceanologyChineseAcademyofSciencesQingdao266071China／／GraduateSchoolChineseAcademyofSciencesBeijing100039China ExperimentalMarineBiologylaboratory InstituteofOceanologyChineseAcademyofSciencesQingdao266071China QingdaoInspectionandQuarantineBureau Qingdao266101China

Penaeidin from Chinese shrimp (Fenneropenaeus chinensis) has proved to be one of the most important antimicrobial peptides in the bodies of animals. The relative quantitative real-time PCR method is developed to study through time, the mRNA expression profile of penaeidin in the muscle and haemocyte tissue of Chinese shrimp infected with vibrio (Vibrio anguillarum) and WSSV (white spot syndrome virus). Research results showed that the same pathogens infection experiments produced similar gene expression profile in different tissues while different expression profiles appeared in the same tissues infected by different exterior pathogens. In vibrio infection experiments, a 'U' like expression profile resulted. Expression levels of penaeidin increased and surpassed the non-stimulated level, indicating that penaeidin from Chinese shrimp has noticeable antimicrobial activities. In WSSV infection experiments, the expression profile appeared as an inverse 'U' with the expression of penaeidin gradually decreasing to below baseline level after 24 h. The expression of antimicrobial peptides gene in mRNA level in response to virus infection in shrimp showed that international mechanisms of virus to haemocytes and microbial to haemocytes are completely different. Decline of penaeidins expression levels may be due to haemocytes being destroyed by WSSV or that the virus can inhibit the expression of penaeidins by yet undiscovered modes. The expression profiles of penaeidin in response to exterior pathogen and the difference of expression profiles between vibrio and WSSV infection provided some clues to further understanding the complex innate immune mechanism in shrimp.

关键词： penaeidin Fenneropenaeus chinensis expression profile WSSV Vibrio anguillarum real-time PCR

Quantitative traits correlative analysis and growth comparison among different populations of bay scallop,Argopecten irradians

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Acta Oceanologica Sinica 2004年第3期23卷 533-540页

作者： LIUBaozhong LIANGYubo LIUXiaolin DONGBo xiangjianhai InstituteofOceanology ChineseAcademyofSciencesQingdao266071China NationalMarineEnvironmentMonitoringCenter Dalian116023China CollegeofAnimalScienceandTechnology NorthwestSci-TechUniversityofAgricultureandForestryYangling712100China

The shell traits and weight traits are measured in cultured populations of bay scallop, Argopecten irradians. The results of regression analysis show that the regression relationships for all the traits are significant (P0.05). The multiple regression equation is obtained to estimate live body weight and tissue weight. The above traits except anterior and posterior auricle length are used for the growth and production comparison among three cultured populations, Duncan's new multiple range procedure analysis shows that all the traits in the Lingshuiqiao (LSQ) population are much more significant than those of the other two populations (P0.05). The results indicate that the LSQ population has a higher growth rate and is expected to be more productive than the other two populations.

关键词： Argopecten irradians quantitative traits correlative analysis growth comparison

Studies on hybridization effects of different geographic populations of Chlamys farreri Ⅱ.The medium-term growth and development of Chlamys farreri populations from China and Russia and their reciprocal crosses

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Acta Oceanologica Sinica 2003年第2期22卷 255-264页

作者： LiuXiaolin ChangYaqing xiangjianhai LiFuhua LiuXianjie BrovkinaElenaPavlovna CollegeofAnimalScienceandTechnology NorthwestSci-TechUniversityofAgricultureandForestryYangling712100China KeyLabofMarineEcologyinAquacultureofMinistryofAgriculture DalianFisheriesUniversityDalian116023China ExperimentalMarineBiologyLaboratory InstituteofOceanologyChineseAcademyofSciencesQingdao266071China KeyLabofMarineEcologyinAquacultureofMinistryofAgricultureofMinistryofAgriculture DalianFisheriesUniversityDalian116023China Far-EastStateTechnicalFisheriesUniversity Vladivostok690000Russia

Based on the research of juvenile (2, 3, 4 months) growth and survival of three populations of two different geographic areas in Chlamys farreri from Russian and China and their F1 hybrids derived from Chinese cultural population (CC) (?) × Russian population (RW) (?) , Chinese wild population (CW) (?) × Russian population (RW) (?), Russian population (RW) (?) × Chinese wild population (CW) (?) , the study of the medium-term (6, 8, 10, 12 months) growth and development of Chlamys farreri was carried out. The four determined results indicated that there existed different extent heterosis (3% -52%) for the growth in three types of F1 hybrids, and the offspring derived from CC(?) ×R(?) had a stronger heterosis among the crosses at the medium-term; the uptrend among traits are wet weight >shell width>shell length> shell height, Chinese cultural population could be recognized as excellent parent, and seasonal variations influence very much on the daily increment and growth rate of each trait of Chlamys farreri and it is only able to survive and could barely grow in winter (6-8 months), but grows fast in temperate season (10-12 months).

关键词： Chlamys farreri population hybridization medium-term growth and development heterosis

Polyploid Breeding and Sex Control in Maricultured Animals

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Bulletin of the Chinese Academy of Sciences 2001年第2期15卷 95-96页

作者： xiangjianhai, Wang Rucai, Wang Zichen, Jiang Weiguo, Zhang Peijun, Wang Qingyin, Guo Ximing, Li Fuhua, Wang Zhaoping, Zhang Guofan, He Maoxian, Xu Cheng, Yang Aiguo, Yang Huiping and Wang Suoan.

Polyploid breeding and sex con-trolling are conducive to lifting mari-culture *** of Insti-tute of Oceanology,CAS and sev-eral other institutes in China Haverecently developed relevanttechniques. This program,starting from1996,focuses on triplold inductionand breeding,tetraplold inductionmechanisms and sex controlling. At the beginning,there were still

关键词： Polyploid Breeding and Sex Control in Maricultured Animals