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线粒体分裂基因dnm1缺失对粟酒裂殖酵母细胞有丝分裂及能量代谢的影响

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北京师范大学学报（自然科学版） 2024年第3期60卷 331-343页

作者：张丁余雯刘梦楠青昕丁祥侯怡铃西华师范大学生命科学学院西南野生动植物资源保护教育部重点实验室四川南充西华师范大学环境科学与工程学院四川南充

在粟酒裂殖酵母细胞中,线粒体分裂蛋白Dnm1是调控线粒体分裂和融合动态过程的关键蛋白.为研究酵母细胞dnm1基因缺失后对有丝分裂和能量代谢的影响,采用活细胞成像技术分析其细胞有丝分裂动力学的变化、RTqPCR技术分析cdc2和cdc13基因的... 详细信息

在粟酒裂殖酵母细胞中,线粒体分裂蛋白Dnm1是调控线粒体分裂和融合动态过程的关键蛋白.为研究酵母细胞dnm1基因缺失后对有丝分裂和能量代谢的影响,采用活细胞成像技术分析其细胞有丝分裂动力学的变化、RTqPCR技术分析cdc2和cdc13基因的转录水平、高效液相色谱质谱联用技术检测其能量代谢物的变化并验证.结果表明dnm1基因缺失后抑制裂殖酵母生长.活细胞成像显示dnm1Δ菌株在有丝分裂间期微管束长度较野生型增长了(0.83±0.70)μm(P<0.01),产生5根微管束的菌株增加、3根微管束的菌株减少.有丝分裂前期dnm1Δ菌株中纺锤体伸长时间延长(0.85±0.02)min(P<0.05),后期时间延长(5.80±1.62)min(P<0.01),后期纺锤体伸长速度减慢0.06μm·min^(-1)(P<0.05),且dnm1Δ菌株出现纺锤体延迟断裂和滞后的染色体分离.高效液相色谱质谱联用技术检测结果表明,dnm1Δ菌株存在辅酶合成缺陷,NADPH含量显著降低(P<0.05),中间代谢产物6-磷酸葡萄糖、6-磷酸果糖、柠檬酸、顺式乌头酸、丙酮酸、异柠檬酸和L-苹果酸相对含量显著降低(P<0.05),出现ATP产生障碍.验证结果显示代谢物分析结果可靠,且dnm1Δ菌株在对数生长期cdc2基因的表达量显著低于野生型.本研究为进一步探寻Dnm1蛋白在细胞有丝分裂中的功能和相关分子机制提供了一定的科学依据.

关键词：裂殖酵母线粒体 dnm1基因有丝分裂能量代谢

Theoretical analysis of frequency modulation-to-amplitude modulation on the final optics and target of the SG Ⅱ-Up laser facility

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High Power Laser Science and Engineering 2024年第1期12卷 84-98页

作者： yujia zhang Wei Fan Jiangfeng Wang Xiaochao Wang Xinghua Lu Dajie Huang Shouying Xu Yanli zhang Mingying Sun Zhaoyang Jiao Shenlei Zhou Xiuqing Jiang Key Laboratory of High Power Laser and Physics Shanghai Institute of Optics and Fine MechanicsChinese Academy of SciencesShanghaiChina Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijingChina

Frequency modulation(FM)-to-amplitude modulation(AM) conversion is an important factor that affects the time±power curve of inertial confinement fusion(ICF) high-power laser facilities. This conversion can impact uniform compression and increase the risk of damage to optics. However, the dispersive grating used in the smoothing by spectral dispersion technology will introduce a temporal delay and can spatially smooth the target. The combined effect of the dispersive grating and the focusing lens is equivalent to a Gaussian low-pass filter, which is equivalent to 8 GHz bandwidth and can reduce the intensity modulation on the target to below 5% with 0.3 nm @ 3 GHz + 20 GHz spectrum phase modulation. The results play an important role in the testing and evaluating of the FM-to-AM on the final optics and the target, which is beneficial for comprehensively evaluating the load capacity of the facility and isentropic compression experiment for ICF.

关键词： dispersion grating frequency modulation-to-amplitude modulation conversion high-power laser facility inertial confinement fusion phase modulation

UWLPIPE:Ultra-wide Bandwidth Low-frequency Pulsar Data Processing Pipeline

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Research in Astronomy and Astrophysics 2024年第7期24卷 145-157页

作者： Ya-Zhou zhang Hai-Long zhang Jie Wang Jian Li Xin-Chen Ye Shuang-Qiang Wang Xu Du Han Wu Ting zhang Shao-Cong Guo Xinjiang Astronomical Observatory Chinese Academy of SciencesUrumqi 830011China University of Chinese Academy of Sciences Beijing 100049China Key Laboratory of Radio Astronomy Chinese Academy of SciencesNanjing 210008China National Astronomical Data Center Beijing 100101China Southeast University Nanjing 211189China

For real-time processing of ultra-wide bandwidth low-frequency pulsar baseband data,we designed and implemented an ultra-wide bandwidth low-frequency pulsar data processing pipeline(UWLPIPE)based on the shared ringbuffer and GPU parallel *** runs on the GPU cluster and can simultaneously receive multiple 128 MHz dual-polarization VDIF data packets preprocessed by the front-end *** aligning the dual-polarization data,multiple 128M subband data are packaged into PSRDADA baseband data or multi-channel coherent dispersion filterbank data,and multiple subband filterbank data can be spliced into wideband data after time *** used the Nanshan 26 m radio telescope with the L-band receiver at964~1732 MHz to observe multiple ***,we processed the data using DSPSR software,and the results showed that each subband could correctly fold out the pulse profile,and the wideband pulse profile accumulated by multiple subbands could be correctly aligned.

关键词： (stars)pulsars general-methods data analysis-techniques miscellaneous

百兆赫兹重频的轨道角动量模式飞秒光纤激光器

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物理学报 2024年第1期73卷 181-192页

作者：吴航陈燎李帅杜禺璠张驰张新亮华中科技大学光学与电子信息学院武汉光电国家研究中心武汉430074

轨道角动量(orbital angular momentum,OAM)模式激光器在大容量通信系统、激光加工、微粒子操作、量子光学领域研究有潜在应用价值.OAM模式飞秒光纤激光器具有结构简单、成本低、峰值功率高等优势而被重点研究.当前OAM模式飞秒光纤激光... 详细信息

轨道角动量(orbital angular momentum,OAM)模式激光器在大容量通信系统、激光加工、微粒子操作、量子光学领域研究有潜在应用价值.OAM模式飞秒光纤激光器具有结构简单、成本低、峰值功率高等优势而被重点研究.当前OAM模式飞秒光纤激光器在重复频率、脉冲宽度、光谱宽度等关键参数上分别都有突破,但性能难以兼得,且重复频率目前在数十MHz.本文基于模式相位匹配原理,制作了大带宽的模式耦合器,结合非线性偏振旋转锁模机理,通过优化腔内的色散,搭建了百兆赫兹重复频率的OAM模式飞秒光纤激光器.实验结果表明,一阶OAM模式光纤激光器的重复频率可达113.6 MHz,脉冲半高全宽98 fs,10 d B带宽可达101 nm;二阶OAM模式光纤激光器的重复频率可达114.9 MHz,脉冲半高全宽60 fs,10 d B带宽可达100 nm.相对于已报道的方案,本文报道的方案在重复频率、脉宽和光谱宽度等关键参数上综合性能较好,有望更广泛地应用于OAM通信、粒子操控等研究领域.

关键词：光纤锁模激光器轨道角动量模式耦合器

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非遗传承与创新视域下吴山庙会文化App设计与研究

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装饰 2024年第2期 136-138页

作者：张煜徐喆朋中国计量大学

本文针对庙会非物质文化遗产在目前所遭遇的传承困境,以杭州庙会作为研究对象,探索传统庙会文化的数字化传承路径,进而为非遗文化发展提供参考。文章立足庙会丰厚的文化价值和当代数字技术,对杭州庙会所承载的物质文化和精神文化进行挖... 详细信息

本文针对庙会非物质文化遗产在目前所遭遇的传承困境,以杭州庙会作为研究对象,探索传统庙会文化的数字化传承路径,进而为非遗文化发展提供参考。文章立足庙会丰厚的文化价值和当代数字技术,对杭州庙会所承载的物质文化和精神文化进行挖掘与再现,打造推动庙会非遗文化传承与创新的App移动端体验设计。最终,本文通过杭州庙会文化研究和数字化实践探索,将其历史文化资料进行数字化保存与展示,以实现文化保护功能;将其传播推广模式与线上平台相融合,实现创新转化功能,为杭州庙会的保护与传承提供新路径,为中国传统庙会文化的传承和推广提供思路。

关键词：非物质文化遗产传承与创新吴山庙会 App 数字化体验设计

铁尾矿基多固废混凝土抗压性能及微观结构分析

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沈阳工业大学学报 2024年第2期46卷 225-232页

作者：张延年林吉森陈昊刘剑平程祥余靖玟沈阳建筑大学土木工程学院辽宁沈阳110168 沈阳工业大学建筑与土木工程学院辽宁沈阳110870

针对铁尾矿堆存困难、综合利用率低和活性低的问题,以铁尾矿钢渣脱硫灰为复合掺合料制备多固废混凝土。通过抗压性能测试,研究复合掺合料掺量、铁尾矿细度对混凝土抗压强度的影响,并利用压汞法(MIP)和背散射电子成像技术(BSE)探究混凝... 详细信息

针对铁尾矿堆存困难、综合利用率低和活性低的问题,以铁尾矿钢渣脱硫灰为复合掺合料制备多固废混凝土。通过抗压性能测试,研究复合掺合料掺量、铁尾矿细度对混凝土抗压强度的影响,并利用压汞法(MIP)和背散射电子成像技术(BSE)探究混凝土的微观结构。结果表明:复合掺合料的掺入对混凝土早期抗压强度影响较大,掺量小于20%的混凝土28 d抗压强度与无掺合料组抗压强度基本持平,30%掺量的混凝土抗压强度随铁尾矿比表面积的增大而先增大后减小;掺入复合掺合料和减小铁尾矿细度能够改善混凝土孔结构和提高界面过渡区的密实度。

关键词：多固废混凝土铁尾矿复合掺合料抗压强度孔结构界面过渡区钢渣脱硫灰

Evaluation of oxaliplatin and tigio combination therapy in locally advanced gastric cancer

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World Journal of Gastrointestinal Surgery 2024年第6期16卷 1709-1716页

作者： Teng Wang Li-yun zhang Department of Gastroenterology Xianyang HospitalYan’an UniversityXianyang 712000Shaanxi ProvinceChina

BACKGROUND Locally advanced gastric cancer(LAGC)is a common malignant *** recent years,neoadjuvant chemotherapy has gradually become popular for the treatment of *** To investigate the efficacy of oxaliplatin combined with a tigio neoadjuvant chemotherapy regimen vs a conventional chemotherapy regimen for *** Ninety patients with LAGC were selected and randomly divided into control and study groups with 45 patients in each group,according to the numerical table *** control group was treated with conventional chemotherapy,and the study group was treated with oxaliplatin combined with tigio-neoadjuvant *** primary outcome measures were the clinical objective response rate(ORR)and surgical resection rate(SRR),whereas the secondary outcome measures were safety and Karnofsky Performance Status *** The ORR in the study group was 80.00%,which was significantly higher than that of the control group(57.78%).In the study group,SRR was 75.56%,which was significantly higher than that of the control group(57.78%).There were 15.56%adverse reactions in the study group and 35.56%in the control *** differences were statistically significant between the two *** The combination of oxaliplatin and tigio before surgery as neoadjuvant chemotherapy for patients with LAGC can effectively improve the ORR and SRR and is safe.

关键词： Locally advanced gastric cancer Oxaliplatin and tigio Neoadjuvant chemotherapy Surgical resection rate Objective response rate Clinical efficacy

Role of autoreactive Tc17 cells in the pathogenesis of experimental autoimmune encephalomyelitis

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Neuroprotection 2024年第1期2卷 49-59页

作者： Yong Peng Xiuli zhang Yandan Tang Shunqing He Guilan Rao Quan Chen Yahui Xue Hong Jin Shu Liu Ziyang Zhou yun xiang Department of Neurology Affliated First Hospitalof HunanTraditional Chinese Medical CollegeZhuzhouHunanChina Department of Neurology The Third Affiliated Hospital of Hunan University of Chinese MedicineZhuzhouHunanChina Science and Technology Innovation Center HunanUniversityof ChineseMedicineChangshaHunanChina

Background:The pathogenesis of multiple sclerosis(MS)and experimental autoimmune encephalomyelitis(EAE-an animal model of MS)is primarily mediated by T ***,recent studies have only focused on interleukin(IL)-17-secreting CD4^(+)T-helper cells,also known as Th17 *** study aimed to compare Th17 cells and IL-17-secreting CD8^(+)T-cytotoxic cells(Tc17)in the context of MS/***:Female C57BL/6 mice were immunized with myelin oligodendrocyte glycoprotein peptides 35-55(MOG35-55),pertussis toxin,and complete Freund's adjuvant to establish the EAE animal model.T cells were isolated from the spleen(12-14 days postimmunization).CD4^(+)and CD8^(+)T cells were purified using isolation kit and then differentiated into Th17 and Tc17,respectively,using MOG35-55 and *** secretion levels of interferon-(IFN-γ)and IL-17 were measured via enzyme-linked immunosorbent assay using cultured CD4^(+)and CD8^(+)T cell *** pathogenicity of Tc17 and Th17 cells was assessed through adoptive transfer(tEAE),with the clinical course assessed using an EAE score(0-5).Hematoxylin and eosin as well as Luxol fast blue staining were used to examine the spinal *** CD8^(+)CD3^(+)and CD4^(+)CD3^(+)cells differentiated into Tc17 and Th17 cells,respectively,were stimulated with MOG35-55 peptide for proliferation ***:The results showed that Tc17 cells(15,951±19855VS.55,709±4196cpm;p<0.050)exhibited a weaker response to highest dose(20μg/mL)MOG35-55 than Th17 ***,this response was not dependent on Th17 *** the 48h stimulation,at the highest dose(20μg/mL)of ***17 cells secreted lower levels of IFN-(280.00±15.00vs.556.67±15.28pg/mL,p<0.050)and IL-17(102.67±5.86 pg/mL vs.288.33±12.58 pg/mL;p<0.050)than Th17 *** patterns were observed for IFN-γsecretion at 96 and ***,Tc17 cell-induced tEAE mice exhibited similar EAE scores to Th17 cell-induced tEAE mice and also showed similar inflammation and demyelinati

关键词： CD8^(+)T cells experimental autoimmune myelitis IFN-y IL-17 multiple sclerosis Tc17 cells

过渡金属(TM=V,Cr,Mn,Fe,Co,Ni)掺杂GeSe的高温铁磁半导体薄膜

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Science China Materials 2024年第1期67卷 279-288页

作者：李德仁张析何文杰彭勇向钢 College of Physics Sichuan UniversityChengdu 610064China College of Physical Science and Technology Lanzhou UniversityLanzhou 730000China

具有高温铁磁性的IV族金属硫族化物磁性半导体薄膜是半导体自旋电子器件所需要的重要材料.本文采用固体源化学气相沉积法制备了一系列过渡金属元素(TM=V,Cr,Mn,Fe,Co和Ni)掺杂GeSe的多晶半导体薄膜样品.磁性测量表明,Mn,Fe和Co掺杂的GeS... 详细信息

具有高温铁磁性的IV族金属硫族化物磁性半导体薄膜是半导体自旋电子器件所需要的重要材料.本文采用固体源化学气相沉积法制备了一系列过渡金属元素(TM=V,Cr,Mn,Fe,Co和Ni)掺杂GeSe的多晶半导体薄膜样品.磁性测量表明,Mn,Fe和Co掺杂的GeSe薄膜表现出较强的铁磁性,居里温度(TC)分别高达277,255和243 K,而V,Cr和Ni掺杂GeSe的多晶薄膜表现出较弱的铁磁性.磁电输运测量表明,Mn,Fe和Co掺杂GeSe的多晶薄膜具有相对较高的空穴浓度,在300 K下高达~1020cm^(-3).基于实验和计算结果的进一步分析表明,Mn,Fe和Co掺杂GeSe的多晶薄膜中的强铁磁性归因于载流子增强的Ruderman-KittelKasuya-Yosida相互作用.我们的研究结果展示了过渡金属掺杂GeSe的磁性半导体薄膜的丰富多样性,并为相关基础研究和器件应用提供了一个新平台.

关键词：半导体薄膜多晶薄膜化学气相沉积法过渡金属元素过渡金属掺杂铁磁半导体空穴浓度磁性测量