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Nano Research 2024年第9期17卷 8250-8260页

作者： Yun Han Di Lan Mengjun Han zihao xia Jiaxiao Zou Zirui Jia College of Chemistry and Chemical Engineering Qingdao UniversityQingdao 266071China School of Materials Science and Engineering Hubei University of Automotive TechnologyShiyan 442002China

The rational design of composition nanostructures and morphologies of the carbon-based composites materials has a significant potential for tuning electromagnetic parameters and thereby improving their performance as electromagnetic wave(EMW)*** this work,the flower-like Cu/Co-NC/MoS_(2)(NC=N-doped carbon skeleton)composites were successfully prepared by employing CuCoZn-ZIF(ZIF=zeolitic imidazolate framework)as precursor with subsequent annealing and hydrothermal *** unique flower-like morphology and electromagnetic synergy strategy between components enable the as-obtained Cu/Co-NC/MoS_(2)composites to exhibit outstanding microwave absorption ***,the minimum reflection loss of Cu/Co-NC/MoS_(2)reaches-54.36 dB at 2.7 *** the thickness reduces to 2.2 mm,the maximum effective absorption bandwidth can be achieved as large as 6.72 *** research develops useful ideas for optimizing multicomponent microwave absorbing materials.

关键词： ternary metal-organic frameworks(MOFs) MoS_(2) flower-like morphology microwave absorption

ZmmiR398b Negatively Regulates Maize Resistance to Sugarcane Mosaic Virus Infection by Targeting ZmCSD2/4/9 and Modulating ROS Accumulation

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ZmmiR398b Negatively Regulates Maize Resistance to Sugarcane...

Ubiquitylome Combined with Proteome Analysis Revealed Glyoxylate Metabolism Pathway Associated Genes were Involved in Resistance to Viral Infection in Maize

中国植物病理学会2024年学术年会

作者： GAO Xinran DU Zhichao xia zihao WU Yuanhua Liaoning Key Laboratory of Plant Pathology College of Plant ProtectionShenyang Agricultural University

MicroRNAs(miRNAs) are widely involved in various biological processes of plants and contribute to plant resistance against various *** this study,upon sugarcane mosaic virus(SCMV) infection,the accumulation of maize(Zea mays L.) miR398b(ZmmiR398 b) was significantly reduced in resistant(Chang7-2) while increased in susceptible(Mo 17) maize inbred *** sequencing analysis coupled with transient co-expression assays revealed that ZmmiR398 b can target Cu/Zn-super oxidase dismut ase2(ZmCSD2),ZmCSD4 and ZmCSD9 in vivo,of which the expression levels were all up-regulated by SCMV infection in Chang7-2 and ***,overexpressing ZmmiR398b (OE398b) transgenicmaize lines exhibited increased susceptibility to SCMV infection probably by increasing reactive oxygen species(ROS) accumulation,which were consistent to ZmCSD2/4/9-silenced maize *** contrast,silencing ZmmiR398 b(STTM398b) through short tandem target mimic(STTM) technology enhanced maize resistance to SCMV infection and decreased ROS ***,copper(Cu) gradient hydroponicexperiments demonstrated that Cu deficiency promoted while Cu sufficiency inhibited SCMV infection by regulating accumulations of ZmmiR398 b and ZmCSD2/4/9 in *** results revealed that manipulating ZmmiR398 b-ZmCSD2/4/9-ROS module provides a prospective strategy for developing SCMV-tolerant maize varieties.

关键词： ZmmiR398b ZmCSD2/4/9 ROS copper SCMV

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Ubiquitylome Combined with Proteome Analysis Revealed Glyoxy...

Two star-shaped small molecule donors based on benzodithiophene unit for organic solar cells

中国植物病理学会2024年学术年会

作者： GUO Huiyan DONG Xue GAO Xinran HAO Kaiqiang GUO Jinxiu LI Jian DING Yueming WANG Zhiping AN Mengnan xia zihao WU Yuanhua Liaoning Key Laboratory of Plant Pathology College of Plant ProtectionShenyang Agricultural University Key Laboratory of Major Agricultural Invasion Biological Monitoring and Control of Shenyang

The co-infection of maize chlorotic mottle virus（MCMV） and sugarcane mosaic virus（SCMV） caused maize lethal necrosis（MLN）,which seriously affected the yield and quality of *** is one of the most important protein post-translational modifications（PTMs） and is widely involved in host-virus ***,the mechanism of ubiquitination modification in regulating maize resistance to viral infection remains largely *** this study,K-ε-GG antibody enrichment method integrated with 4 D mass spectrometry was used to analyze ubiquitylome and proteome of maize infected by MCMV and/or SCMV.A total of 2593 differentially expressed proteins（DEPs） and3681 quantified lysine ubiquitination（Kub） sites were *** functional analysis showed that these ubiquitinated DEPs were mainly involved in photosynthesis,starch and sucrose metabolism,glyoxylate and dicarboxylate metabolism,and fatty acid ***-induced gene silencing（VIGS） assay was used to investigate the function of three key genes in glyoxylate metabolism pathway in *** results showed that ZmHPRl and ZmHPR2 were required for virus infection,while ZmGOXl played important roles in resistance to virus *** addition,we found that exogenous application of sodium sulfide（NaS） could up-regulate the expression of ZmGOX1 to effectively inhibit viral *** findings provide novel insights into the roles of ubiquitination in the regulation of maize resistance to viral infection.

关键词： ubiquitylome proteome sugarcane mosaic virus(SCMV) maize chlorotic mottle virus(MCMV) maize lethal necrosis(MLN) glyoxylate metabolism maize

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Chinese Chemical Letters 2022年第1期33卷 247-251页

作者： Jun Xu Jinsheng Zhang Daobin Yang Kuibao Yu Dandan Li zihao xia Ziyi Ge Ningbo Institute of Materials Technology and Engineering Chinese Academy of SciencesNingbo 315201China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China Nano Science and Technology Institute University of Science and Technology of ChinaSuzhou 215123China

Star-shaped small molecules have attracted great attention for organic solar cells(OSCs)because they have three-dimensional charge-transport characteristics,strong light absorption capacities and easily tunable energy ***,three-and four-armed star-shaped small molecule donors,namely BDT-3 Th and BDT-4 Th,respectively,have been successfully designed and synthesized,which used benzodithiophene(BDT)as the central *** two star-shaped intermediates(2 a and 2 b)could be simultaneously obtained by one-step of Suzuki coupling,and 1,2-dimethoxyethane played a key role in the Suzuki *** of them have excellent thermal stability,good solubility and broad ***-armed BDT-4 Th shows a slightly higher extinction coefficient,a deeper HOMO energy level and an obviously better phase separation morphology when blended with Y6 than three-armed BDT-3 *** a result,increased power conversion efficiency(PCE)of 5.83%is obtained in the BDT-4 Th:Y6-based OSC devices,which is obviously higher than that of the BDT-3 Th:Y6-based devices(PCE=3.78%).To the best of our knowledge,this is the highest PCE among the BDT-based star-shaped donors-based *** result provides an effective strategy to obtain star-shaped small molecule donor materials for high efficient organic solar cells.

关键词： Star-shaped molecules Benzodithiophene Suzuki coupling Donor materials Small molecules Organic solar cells

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新农科背景下植物医生特长班建设模式探索

安徽农学通报 2024年第10期30卷 116-120页

作者：魏松红高萍董辉夏子豪李修伟沈阳农业大学植物保护学院辽宁沈阳110866

新农科建设适应了农业现代化发展的需要,适应了高等农林教育发展改革的需要。在新农科建设背景下,植物保护专业的人才培养面临新的机遇和挑战。本文对植物保护与植物医学的发展现状进行分析,以植物医生特长班为平台,对其建设思路进行分... 详细信息

新农科建设适应了农业现代化发展的需要,适应了高等农林教育发展改革的需要。在新农科建设背景下,植物保护专业的人才培养面临新的机遇和挑战。本文对植物保护与植物医学的发展现状进行分析,以植物医生特长班为平台,对其建设思路进行分析,并探讨植物医生特长班建设模式,具体包括植物医生特长班总体设计、培养方案制定、教学团队建设、一流课程资源建设和虚拟仿真实验教学基地建设等方面,为新农科建设人才培养提供参考。

关键词：新农科植物医生特长班植物保护

转录因子NbMYB1R1通过促进活性氧积累抑制病毒侵染

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中国农业科学 2024年第8期57卷 1490-1505页

作者：姜兴林于连伟付涵艾妞崔荧钧李好海夏子豪袁虹霞李洪连杨雪施艳河南农业大学植物保护学院河南省植物保护新技术推广协会河南省植物保护检疫站沈阳农业大学植物保护学院河南省粮食作物协同创新中心小麦玉米作物学国家重点实验室河南农业大学作物学博士后流动站

【背景】黄瓜绿斑驳花叶病毒（cucumber green mottle mosaic virus,CGMMV）是我国重要的检疫性植物病毒，严重降低了世界范围内蔬菜以及瓜类的产量。MYB蛋白家族庞大，功能多样，存在于所有真核生物中。大多数MYB作为转录因子控制植物... 详细信息

【背景】黄瓜绿斑驳花叶病毒（cucumber green mottle mosaic virus,CGMMV）是我国重要的检疫性植物病毒，严重降低了世界范围内蔬菜以及瓜类的产量。MYB蛋白家族庞大，功能多样，存在于所有真核生物中。大多数MYB作为转录因子控制植物的发育和代谢，并对植物应对生物和非生物胁迫反应起重要的调控作用。前期研究显示CGMMV侵染后可以显著上调寄主转录因子基因NbMYB1R1的表达。【目的】明确NbMYB1R1参与CGMMV侵染的机制，为CGMMV病害防控提供理论依据。【方法】运用MEGA 7.0构建系统进化树，对NbMYB1R1的氨基酸序列进行系统进化分析；通过构建NbMYB1R1的荧光表达载体，转化GV3101农杆菌后浸润本氏烟叶片，激光共聚焦显微镜观察其亚细胞定位；利用qRT-PCR技术分析NbMYB1R1在CGMMV侵染不同时期的转录水平及NbMYB1R1沉默烟草植株中活性氧（reactive oxygen species,ROS）相关基因的转录变化；通过沉默NbMYB1R1及下游调控基因NbAOX1a和NbAOX1b，分析NbMYB1R1及下游调控基因NbAOX1a和NbAOX1b在CGMMV侵染过程中的作用；瞬时过表达NbMYB1R1和NbMYB1R1关键氨基酸突变体，分析NbMYB1R1对CGMMV侵染的影响；使用台盼蓝染色以及DAB染色观察瞬时过表达NbMYB1R1造成的细胞死亡是否与程序性细胞死亡（programmed cell death,PCD）以及ROS的积累有关；运用酵母双杂交技术验证NbMYB1R1是否与CGMMV相关蛋白互作。【结果】系统进化树分析表明，NbMYB1R1属于1R MYB大类并与多种烟草的MYB转录因子同源性极高；亚细胞定位结果显示NbMYB1R1定位于细胞核；在CGMMV侵染的烟草植株中，NbMYB1R1的转录水平对比健康植株有明显变化，在CGMMV侵染8、12 d时NbMYB1R1的转录水平显著上调；在沉默内源基因NbMYB1R1的植株上接种CGMMV,3 d后NbMYB1R1沉默植株系统叶出现斑驳、卷曲症状，而对照植株在3.5 d时才出现症状；同时CGMMV CP mRNA水平和蛋白水平检测结果也表明沉默NbMYB1R1可以有效促进CGMMV的积累；在本氏烟叶片瞬时过表达NbMYB1R1及其突变体3 d时检测CGMMV蛋白水平表达量，结果显示过表达NbMYB1R1可以有效抑制CGMMV的侵染，DNA结合结构域缺失后会减轻NbMYB1R1对CGMMV的抑制；台盼蓝和DAB染色结果表明，在瞬时过表达NbMYB1R1蛋白后导致ROS积累并引起细胞死亡；在沉默内源基因NbMYB1R1的植株叶片上检测ROS相关基因的转录水平，发现交替氧化酶基因AOX1a、AOX1b转录水平显著上调；在沉默内源基因NbAOX1a和NbAOX1b的植株上接种CGMMV,4 d后NbAOX1a和NbAOX1b沉默植株系统叶出现斑驳、卷曲症状，而对照植株在3.5 d时就出现症状；同时CGMMV CP mRNA水平和蛋白水平检测结果也表明沉默NbAOX1a和NbAOX1b可以有效抑制CGMMV的积累；酵母双杂交结果显示NbMYB1R1不与CGMMV编码蛋白直接相互作用。【结论】随着CGMMV侵染，防御相关基因NbMYB1R1表达上调，从而抑制下游基因AOX1a、AOX1b的表达并激活细胞内产生ROS抑制病毒侵染，但NbMYB1R1不是通过与CGMMV病毒蛋白直接互作而产生此作用。由此可见，NbMYB1R1在CGMMV侵染过程中发挥了重要作用。

关键词：黄瓜绿斑驳花叶病毒 Nb MYB1R1 转录因子致病机制活性氧

All‑Covalent Organic Framework Nanofilms Assembled Lithium‑Ion Capacitor to Solve the Imbalanced Charge Storage Kinetics

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Nano-Micro Letters 2024年第6期16卷 246-260页

作者： xiaoyang Xu Jia Zhang zihao Zhang Guandan Lu Wei Cao Ning Wang Yunmeng xia Qingliang Feng Shanlin Qiao College of Chemistry and Pharmaceutical Engineering Hebei University of Science and TechnologyShijiazhuang 050018People’s Republic of China School of Chemistry and Chemical Engineering Northwestern Polytechnical UniversityXi’an 710072People’s Republic of China Hebei Engineering Research Center of Organic Solid Photoelectric Materials for Electronic Information Shijiazhuang 050018People’s Republic of China

Free-standing covalent organic framework(COFs)nanofilms exhibit a remarkable ability to rapidly intercalate/de-intercalate Li^(+) in lithium-ion batteries,while simultaneously exposing affluent active sites in *** development of these nanofilms offers a promising solution to address the persistent challenge of imbalanced charge storage kinetics between battery-type anode and capacitor-type cathode in lithium-ion capacitors(LICs).Herein,for the first time,custom-made COFBTMB-TP and COFTAPB-BPY nanofilms are synthesized as the anode and cathode,respectively,for an all-COF nanofilm-structured *** COFBTMB-TP nanofilm with strong electronegative–CF3 groups enables tuning the partial electron cloud density for Li^(+) migration to ensure the rapid anode kinetic *** thickness-regulated cathodic COFTAPB-BPY nanofilm can fit the anodic COF nanofilm in the *** to the aligned 1D channel,2D aromatic skeleton and accessible active sites of COF nanofilms,the whole COFTAPB-BPY//COFBTMB-TP LIC demonstrates a high energy density of 318 mWh cm^(−3) at a high-power density of 6 W cm^(−3),excellent rate capability,good cycle stability with the capacity retention rate of 77%after *** COFTAPB-BPY//COFBTMB-TP LIC represents a new benchmark for currently reported film-type LICs and even film-type *** being comprehensively explored via ex situ XPS,7Li solid-state NMR analyses,and DFT calculation,it is found that the COFBTMB-TP nanofilm facilitates the reversible conversion of semi-ionic to ionic C–F bonds during lithium ***-TP exhibits a strong interaction with Li^(+) due to the C–F,C=O,and C–N bonds,facilitating Li^(+) desolation and absorption from the *** work addresses the challenge of imbalanced charge storage kinetics and capacity between the anode and cathode and also pave the way for future miniaturized and wearable LIC devices.

关键词： Covalent organic frameworks Lithium-ion capacitor Charge storage kinetic

OBE理念与翻转课堂融合的植物病理学课程教学改革与探索——以植物病害生物防治课程为例

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创新创业理论研究与实践 2024年第16期7卷 165-167页

作者：范海燕朱晓峰杨宁段玉玺夏子豪陈立杰沈阳农业大学植物保护学院辽宁沈阳110866

植物病害生物防治课程是植物保护专业重要的专业选修课之一,对新农科一流植物保护人才培养至关重要。课程组秉承OBE(成果导向教育)理念,融合翻转课堂,针对课程的教学现状,从教学内容的更新、思政元素资源库的构建、教学手段的改进及多... 详细信息

植物病害生物防治课程是植物保护专业重要的专业选修课之一,对新农科一流植物保护人才培养至关重要。课程组秉承OBE(成果导向教育)理念,融合翻转课堂,针对课程的教学现状,从教学内容的更新、思政元素资源库的构建、教学手段的改进及多元化考核方式的改革等多个维度进行改革与探索,以期提高课程教学质量,进一步提升学生自主学习能力、专业实践能力和创新能力。

关键词： OBE 翻转课堂教学内容思政元素教学手段考核方式

单倍体供者与HLA相合供者HSCT治疗儿童MDS的疗效和安全性对比

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中国小儿血液与肿瘤杂志 2024年第1期29卷 44-49页

作者：戴银亮夏子豪胡轶歆高莉高伟李捷卢俊肖佩芳胡绍燕苏州大学附属儿童医院苏州215025

目的评价单倍体供者移植作为替代方案用于异基因造血干细胞移植(allo-HSCT)治疗儿童骨髓增生异常综合征(MDS)的疗效及安全性。方法对2013年4月1日—2022年8月30日至苏州大学附属儿童医院接受allo-HSCT的22例儿童进展型MDS进行回顾性分析... 详细信息

目的评价单倍体供者移植作为替代方案用于异基因造血干细胞移植(allo-HSCT)治疗儿童骨髓增生异常综合征(MDS)的疗效及安全性。方法对2013年4月1日—2022年8月30日至苏州大学附属儿童医院接受allo-HSCT的22例儿童进展型MDS进行回顾性分析,其中全相合移植6例,单倍体移植16例。结果22例患儿均实现中性粒细胞植入,全相合组和单倍体组中性粒细胞植入中位时间分别为移植后11(10~12)天和11(9~17)天,血小板植入中位时间分别为移植后11(8~16)天和12(7~28)天。两组aGVHD的发生率分别为50%和100%(P=0.013),Ⅱ~Ⅳ度aGVHD发生率分别为0%和81%(P=0.001),aGVHD发生时间、Ⅲ~Ⅳ度aGVHD、cGVHD、广泛型cGVHD发生率无统计学意义。单倍体组移植后围植入综合征发生率为81%,显著高于全相合组的17%(P=0.011)。全相合组和单倍体组在CMV血症、EBV血症、出血性膀胱炎、肺部感染及神经系统并发症发生率上差异无统计学意义。至随访截止日期,全相合组中位随访时间为20.82(8.00~68.00)个月,单倍体组中位随访时间为21.38(2.77~69.77)个月,两组5年总体生存率分别为100%和(72.7±17.7)%,两组差异无统计学意义。结论单倍体移植与同胞全相合移植后细胞植入、严重的移植相关并发症发生率、免疫重建及长期存活率无明显差异,因此,对于无全相合供者的儿童MDS患者,单倍体供体移植可作为合适的替代方案。

关键词：造血干细胞移植儿童骨髓增生异常综合征单倍体供者移植

Visible light mediated oxo-difluoroalkylation of styrenes with DMSO as oxidant

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Visible light mediated oxo-difluoroalkylation of styrenes wi...