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Molecular cloning,characterization and promoter analysis of LbgCWIN1 and its expression profiles in response to exogenous sucrose during in vitro bulblet initiation in lily

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Horticultural Plant Journal 2024年第2期10卷 545-555页

作者： Cong Gao Shiqi Li Yunchen Xu Yue Liu yiping xia Ziming Ren Yun Wu Genomics and Genetic Engineering Laboratory of Ornamental Plants Institute of Landscape ArchitectureCollege of Agriculture and BiotechnologyZhejiang UniversityHangzhouZhejiang 310058China Laboratory of Flower Bulbs Department of Landscape ArchitectureSchool of Civil Engineering and ArchitectureZhejiang Sci-Tech UniversityHangzhouZhejiang 310018China

Lily(Lilium spp.) is an important ornamental flower, which is mainly propagated by bulbs. Cell wall invertases(CWINs), which catalyze the irreversibly conversion of sucrose into glucose and fructose in the extracellular space, are key enzymes participating in sucrose allocation in higher plants. Previous studies have shown that CWINs play an essential role in bulblet initiation process in bulbous crops, but the underlying molecular mechanism remains unclear. Here, a CWIN gene of Lilium brownii var. giganteum(Lbg) was identified and amplified from genomic DNA. Quantitative RT-PCR assays revealed that the expression level of LbgCWIN1 was highly upregulated exactly when the endogenous starch degraded in non-sucrose medium during in vitro bulblet initiation in Lbg. Phylogenetic relationship, motif, and domain analysis of LbgCWIN1 protein and CWINs in other plant species showed that all sequences of these CWIN proteins were highly conserved. The promoter sequence of LbgCWIN1 possessed a number of alpha-amylase-, phytohormone-, light-and stress-responsive cis-elements. Meanwhile, β-glucuronidase(GUS) assay showed that the 459 bp upstream fragment from the translational start site displayed maximal promoter activity. These results revealed that LbgCWIN1 might function in the process of in vitro bulblet initiation and be in the response to degradation of endogenous starch.

关键词： Lilium brownii var.giganteum LbgCWIN1 Phylogenetic analysis Promoter analysis Bulblet initiation

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观赏植物糖转运蛋白研究进展

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浙江大学学报（农业与生命科学版） 2024年第1期50卷 12-24页

作者：黄子洋刘洁康婕任梓铭崔祺李东泽夏宜平马斯吴昀浙江理工大学建筑工程学院风景园林系球根花卉实验室浙江杭州310018 浙江大学农业与生物技术学院观赏植物基因组与基因工程实验室浙江杭州310058 中国农业大学园艺学院北京100193

观赏植物因其花、叶、果实等具有良好的观赏价值而在环境美化中得到广泛应用。糖转运蛋白在观赏植物生长发育、开花结实以及逆境胁迫响应等方面发挥重要作用,因此,研究糖转运蛋白对观赏植物产生的影响具重要意义。单糖转运蛋白、蔗糖转... 详细信息

观赏植物因其花、叶、果实等具有良好的观赏价值而在环境美化中得到广泛应用。糖转运蛋白在观赏植物生长发育、开花结实以及逆境胁迫响应等方面发挥重要作用,因此,研究糖转运蛋白对观赏植物产生的影响具重要意义。单糖转运蛋白、蔗糖转运蛋白和糖外排转运蛋白是目前植物中发现的3大类糖转运蛋白。本文对这3类糖转运蛋白成员的分类及基本特性进行了比较和讨论,重点介绍了观赏植物中糖转运蛋白功能和调控的最新研究进展,以期为今后利用基因工程技术改良观赏植物并提高其观赏性及适应性提供理论依据。

关键词：观赏植物单糖转运蛋白蔗糖转运蛋白糖外排转运蛋白

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园艺植物韧皮部卸载研究进展

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浙江农林大学学报 2024年第2期41卷 437-446页

作者：刘洁黄子洋康婕崔祺任梓铭马斯楼建华夏宜平吴昀浙江理工大学建筑工程学院风景园林系球根花卉实验室浙江杭州310018 中国农业大学园艺学院北京100083 杭州植物园杭州西湖园林科学研究院浙江杭州310000 浙江大学农业与生物技术学院观赏植物基因组与基因工程实验室浙江杭州310058

植物韧皮部卸载包括一系列复杂的过程,韧皮部卸载在园艺植物(果树、蔬菜、观赏植物)同化物的运输和分配中具有重要作用。本研究概述了韧皮部卸载的主要途径,并重点综述了韧皮部卸载在园艺植物生长发育方面的主要研究成果,内容包括:①韧... 详细信息

植物韧皮部卸载包括一系列复杂的过程,韧皮部卸载在园艺植物(果树、蔬菜、观赏植物)同化物的运输和分配中具有重要作用。本研究概述了韧皮部卸载的主要途径,并重点综述了韧皮部卸载在园艺植物生长发育方面的主要研究成果,内容包括:①韧皮部运输的主要糖分物质;②韧皮部卸载方式;③韧皮部卸载研究方法;④园艺植物韧皮部卸载研究。随着研究的深入和新技术的出现,园艺植物韧皮部卸载过程中涉及的关键酶与蔗糖转运蛋白等需得到更深入地阐释,进一步助力解析植物韧皮部卸载参与园艺植物生长发育的分子调控机制,并有望为更多的植物韧皮部卸载研究提供新的思路。

关键词：园艺植物韧皮部卸载共质体卸载质外体卸载

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Unexpected de-twinning of strongly-textured Ti mediated by local stress

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Journal of Materials Science & Technology 2022年第30期125卷 231-237页

作者： Kesong Miao Meng Huang yiping xia Hao Wu Qing Liu Guohua Fan Key Laboratory for Light-weight Materials Nanjing Tech UniversityNanjing 211816China School of Materials Science and Engineering Harbin Institute of TechnologyHarbin 150001China

As one of the heterostructures,the layered structure has attracted extensive research interests as it could regulate the deformation mechanisms[1-3].A remarkable combination of strength and ductility has been achieved in layered metals,including CuCu[4-6],Cu-Ni[7],Cu-Al[8,9],Cu-Ti[10],Ti-Ti[11,12],and Ti-Al[13-15].During deformation,mechanical mismatch(discrepancies of yield strength,work hardening,etc.)between component layers leads to mutual constraints,which generates local stress and local strain that deviate from the applied counterparts[16-18].As a result,the activation of additional deformation mechanisms is promoted in layered metals,such as slip systems with low Schmid factor or high critical resolved shear stress[19,20].

关键词： stress deformation strength

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超声法制备非金属及其化合物量子点研究进展

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光电工程 2024年第4期51卷 1-16页

作者：张泰玮胡坤李国彬杨奥夏溢坪李学铭唐利斌杨培志云南师范大学能源与环境科学学院可再生能源材料先进技术与制备教育部重点实验室云南昆明650500 昆明物理研究所云南昆明650223 云南省先进光电材料与器件重点实验室云南昆明650223

量子点(Quantum dots,QDs)晶粒直径在1~20 nm之间,是一种零维的纳米材料,具有尺寸可调、激发光谱宽、高量子效率、宽波长范围、光化学稳定性高、不易光解等优异的光学特性。当量子点的尺寸接近或小于激子波尔半径时,原有的连续能带结构... 详细信息

量子点(Quantum dots,QDs)晶粒直径在1~20 nm之间,是一种零维的纳米材料,具有尺寸可调、激发光谱宽、高量子效率、宽波长范围、光化学稳定性高、不易光解等优异的光学特性。当量子点的尺寸接近或小于激子波尔半径时,原有的连续能带结构量子化,其性质也发生显著变化,表现出优异的光电性能。本综述介绍了量子点的制备方法,其中,超声法作为一种常见的“自上而下”方法,因操作简单、对环境友好等优点被广泛使用。首先,概述了利用超声法制备非金属和非金属化合物量子点的机理和表征技术,并分析了分散剂、超声功率和时间等因素对其尺寸及形貌的影响。随后,对超声法制备的非金属和非金属化合物量子点在激光器、太阳能电池等领域的应用展开了讨论,并针对目前研究存在的一些挑战和难题,提出了自己的观点和见解。最后,对超声法制备非金属单质及非金属化合物量子点进行了总结和展望。

关键词：量子点纳米材料超声法非金属单质非金属化合物

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Effects of the layer thickness ratio on the enhanced ductility of laminated aluminum

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Journal of Materials Science & Technology 2022年第16期111卷 256-267页

作者： yiping xia Hao Wu Kesong Miao Xuewen Li Chao Xu Lin Geng Honglan Xie Guohua Fan School of Materials Science and Engineering Harbin Institute of TechnologyHarbin 150001China Key Laboratory for Light-weight Materials Nanjing Tech UniversityNanjing 211816China Shanghai Synchrotron Radiation Facility Shanghai Institute of High Energy Applied PhysicsShanghai 200000China

The layered structural parameters have been reported to be critical for tuning the tensile properties of laminated ***,we investigated the effects of the thickness ratio(rc/f)of coarse-grained layers(CLs)to fine-grained layers(FLs)on the enhanced ductility of the laminated *** local strain evolution demonstrates that the strain delocalization ability of laminated Al is improved with the decrease of rc/*** interfacial strain gradients,which can produce extra work hardening,gradually approach and cover the CLs with the rc/fdecreasing,explaining the trend of uniform elongation in laminated Al with various rc/*** integrated fracture morphology characterization reveals that the increase of the rc/fleads to an improvement in the tolerance of the interfacial microcracks,which is corresponding to the variation of fracture elongation in the laminated ***,there is an evident transition of transverse propagation path of interfacial microcracks from the CLs to FLs with increasing the rc/*** on a geometrical criterion of microcracks connectivity,the preferential transverse propagation path of interfacial microcracks in these laminated Al was *** calculation based on this criterion also predicted the critical rc/fcorresponding to the optimal combination of strength and fracture *** work deepens the understanding of the role of structural parameters of laminated metals in achieving the strength and ductility synergy.

关键词： Layered structure Structural parameter Digital image correlation Crack propagation

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基于主成分分析和聚类分析的赏食兼用LA百合品种评价

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浙江农业科学 2024年第7期65卷 1639-1645页

作者：易博徐蕴晨吴昀余小华袁粲吕群丹夏宜平丽水绿地华东药用植物园有限公司浙江丽水323000 浙江大学园林研究所浙江杭州310058 浙江理工大学风景园林系球根花卉实验室浙江杭州310018 丽水市农林科学研究院浙江丽水323000

百合属(Lillium spp.)植物种质资源丰富,兼具观赏、食用、药用价值,但品种选育与应用研究大多聚焦于观赏百合,而食用百合品种长期较单一,对于百合的多功能性研究也较缺失。该研究以10个LA百合品种为材料,进行观赏性状(花期、花色、株高... 详细信息

百合属(Lillium spp.)植物种质资源丰富,兼具观赏、食用、药用价值,但品种选育与应用研究大多聚焦于观赏百合,而食用百合品种长期较单一,对于百合的多功能性研究也较缺失。该研究以10个LA百合品种为材料,进行观赏性状(花期、花色、株高、花苞数)和产量性状(母鳞茎鲜重增量、母鳞茎直径增量、小鳞茎数量、小鳞茎鲜重),以及抗病性的综合评价研究,为开发赏食兼用型多功能百合提供理论基础。主成分分析结果显示,帝王黄、红妆和蜜苏卡综合得分位列前三;聚类分析将10个LA百合品种分为3类,与主成分分析结果基本一致,证实主成分分析的可信度。进一步选择综合评价最高的百合品种帝王黄鳞茎,测定其矿质养分、蛋白质、氨基酸、淀粉和粗纤维等营养成分含量,与江浙地区主产食用百合卷丹相比,总体营养价值相仿。因此,帝王黄等LA百合品种具有赏食兼用潜力。

关键词：百合主成分分析聚类分析评价体系赏食兼用

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Flow Behaviour Analysis and Experimental Investigation for Emitter Micro-channels

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Chinese Journal of Mechanical Engineering 2012年第4期25卷 729-737页

作者： WEI Zhengying CAO Meng LIU xia TANG yiping LU Bingheng State Key Lab for Manufacturing System Engineering Xi 'an Jiaotong University Xi 'an 710049 China

The existing research of the flow behavior in emitter micro-channels mainly focuses on the single-phase flow *** the recent micro-particle image velocimetry（PIV） experimental research on the flow characteristics in various micro-channels mainly focuses on the single-phase fluid ***,using an original-size emitter prototype to perform the experiments on the two-phase flow characteristics of the labyrinth channels is seldom *** this paper,the practical flow of water,mixed with sand escaped from filtering,in the labyrinth channel,is *** some research work on the clogging mechanism of the labyrinth channel＇s structure is *** fluid dynamics（CFD） analysis has been performed on liquid-solid two-phase flow in labyrinth-channel *** on flow visualization technology-micro-PIV,the flow in labyrinth channel has been photographed and *** path line graph and velocity vector graph are obtained through the post-treatment of experimental *** graphs agree well with CFD analysis results,so CFD analysis can be used in optimal design of labyrinth-channel *** the optimized anti-clogging structures of the rectangular channel and zigzag channel have been designed *** CFD numerical simulation and the micro-PIV experiments analysis on labyrinth-channel emitter,make the ＂black box＂ of the flow behavior in the emitter channel ***,the proposed research promotes an advanced method to evaluate the emitter＇s performance and can be used to conducting the optimal design of the labyrinth-channel emitters.

关键词： drip irrigation emitter labyrinth channel two-phase flow analysis anti-clogging micro-PIV

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Molecular cloning,characterization and expression analysis of three key starch synthesis-related genes from the bulb of a rare lily germplasm,Lilium brownii ***

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2021年第6期22卷 476-491页

作者： Yun WU Minyi SUN Shiqi LI Ruihan MIN Cong GAO Qundan LYU Ziming REN yiping xia Department of Landscape Architecture School of Civil Engineering and ArchitectureZhejiang Sci-Tech UniversityHangzhou 310018China Genomics and Genetic Engineering Laboratory of Ornamental Plants College of Agriculture and BiotechnologyZhejiang UniversityHangzhou 310058China Chemical Biology Center Lishui Institute of Agriculture and Forestry SciencesLishui 323000China

Starch is the predominant compound in bulb scales,and previous studies have shown that bulblet development is closely associated with starch ***,how starch synthesis affects bulbification at the molecular level is *** this study,we demonstrate that Lilium brownii ***,a wild lily with a giant bulb in nature,and ***,the native species,have different starch levels and characteristics according to cytological and ultra-structural *** cloned the complete sequence of three key gene-encoding enzymes(LbgAGPS,LbgGBSS,and LbgSSⅢ)during starch synthesis by rapid amplification of 5’and 3’complementary DNA(cDNA)ends(RACE)*** analysis revealed that the proteins deduced by these genes contain the canonical conserved *** phylogenetic trees confirmed the evolutionary relationships with proteins from other species,including monocotyledons and *** transcript levels of various tissues and time course samples obtained during bulblet development uncovered relatively high expression levels in bulblets and gradual increase expression accompanying bulblet ***,a set of single nucleotide polymorphisms(SNPs)was discovered in the AGPS genes of four lily genotypes,and a purifying selection fashion was predicted according to the non-synonymous/synonymous(Ka/Ks)*** together,our results suggested that key starch-synthesizing genes might play important roles in bulblet development and lead to distinctive phenotypes in bulblet size.

关键词： Lilium Starch synthesis Bulblet development Single nucleotide polymorphism(SNP) Expression pattern

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IMpower210:A phase Ⅲ study of second-line atezolizumab vs. docetaxel in East Asian patients with non-small cell lung cancer

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Chinese Journal of Cancer Research 2024年第2期36卷 103-113页

作者： Yi-Long Wu Shun Lu Gongyan Chen Jianxing He Jifeng Feng yiping Zhang Liyan Jiang Hongming Pan Jianhua Chang Jian Fang Amy Cai Lilian Bu Jane Shi Jinjing xia Guangdong Lung Cancer Institute Guangdong Provincial Key Laboratory of Translational Medicine in Lung CancerGuangdong Provincial People's Hospital&Guangdong Academy of Medical SciencesSouthern Medical UniversityGuangzhou 510080China Shanghai Lung Cancer Center Shanghai Chest HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200025China Department of Respiration Harbin Cancer HospitalHarbin Medical UniversityHarbin 150081China Department of Thoracic Oncology and Surgery the First Affliated Hospital of Guangzhou Medical UniversityGuangzhou 510120China Department of Medical Oncology Jiangsu Cancer HospitalNanjing 210009China Department of Medical Oncology Zhejiang Cancer HospitalHangzhou 330022China Department of Pulmonary Medicine Shanghai Chest HospitalShanghai Jiao Tong UniversityShanghai 200030China Department of Medical Oncology Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China Department of Medical Oncology Shenzhen HospitalCancer Hospital of Chinese Academy of Medical SciencesShenzhen 518116China Department of Thoracic Oncology Beijing Cancer HospitalBeijing 100142China Product Development Shanghai Roche Pharmaceutical LtdShanghai 201203China

Objective: IMpower210(NCT02813785) explored the efficacy and safety of single-agent atezolizumab *** as second-line treatment for advanced non-small cell lung cancer(NSCLC) in East Asian ***: Key eligibility criteria for this phase Ⅲ, open-label, randomized study included age ≥18 years;histologically documented advanced NSCLC per the Union for International Cancer Control/American Joint Committee on Cancer staging system(7th edition);Eastern Cooperative Oncology Group performance status of 0 or 1;and disease progression following platinum-based chemotherapy for advanced or metastatic NSCLC. Patients were randomized 2:1 to receive either atezolizumab(1,200 mg) or docetaxel(75 mg/m^(2)). The primary study endpoint was overall survival(OS) in the intention-to-treat(ITT) population with wild-type epidermal growth factor receptor expression(ITT EGFR-WT) and in the overall ITT ***: Median OS in the ITT EGFR-WT population(n=467) was 12.3 [95% confidence interval(95% CI),10.3-13.8] months in the atezolizumab arm(n=312) and 9.9(95% CI, 7.8-13.9) months in the docetaxel arm[n=155;stratified hazard ratio(HR), 0.82;95% CI, 0.66-1.03]. Median OS in the overall ITT population was 12.5(95% CI, 10.8-13.8) months with atezolizumab treatment and 11.1(95% CI, 8.4-14.2) months(n=377) with docetaxel treatment(n=188;stratified HR, 0.87;95% CI, 0.71-1.08). Grade 3/4 treatment-related adverse events(TRAEs) occurred in 18.4% of patients in the atezolizumab arm and 50.0% of patients in the docetaxel ***: IMpower210 did not meet its primary efficacy endpoint of OS in the ITT EGFR-WT or overall ITT populations. Atezolizumab was comparatively more tolerable than docetaxel, with a lower incidence of grade3/4 TRAEs.

关键词： Atezolizumab East Asia non-small cell lung cancer programmed death-ligand 1 inhibitors monoclonal antibody

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