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加味逍遥散通过外泌体miRNA途径发挥抗肝癌作用

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中国组织工程研究 2025年第19期29卷 4052-4062页

作者：刘晓明程金来黎如霜李妞妞秦秋云夏猛姚春广西中医药大学广西壮族自治区南宁市530200 广西中医药大学第一附属医院广西壮族自治区南宁市530200

背景:在课题组先前研究中发现加味逍遥散具有明显的抗肝癌作用,但具体作用机制不明。目的:基于高通量测序结合生物信息学,探讨加味逍遥散对二乙基亚硝胺慢性诱导的原发性肝癌模型大鼠血浆外泌体miRNA水平的调节作用。方法:将SD大鼠随机... 详细信息

背景:在课题组先前研究中发现加味逍遥散具有明显的抗肝癌作用,但具体作用机制不明。目的:基于高通量测序结合生物信息学,探讨加味逍遥散对二乙基亚硝胺慢性诱导的原发性肝癌模型大鼠血浆外泌体miRNA水平的调节作用。方法:将SD大鼠随机分为空白对照组、肝癌模型组、加味逍遥散组。以二乙基亚硝胺持续给药12周诱导肝癌模型,从第17周开始,加味逍遥散组大鼠给予加味逍遥散灌服,每日1次,直至第20周末停药,空白对照组和肝癌模型组大鼠给予等量生理盐水灌胃,通过检测大鼠肝组织的形态结构、肝癌标志物Glypican-3蛋白和血清甲胎蛋白表达验证抗肝癌效应。使用超速离心法分离提取各组大鼠血浆外泌体,利用高通量测序技术筛选出大鼠血浆外泌体中差异表达的miRNA,生物信息学预测加味逍遥散通过肝癌血浆来源外泌体miRNA发挥抗肝癌作用的潜在生物标志物,并进行功能分析。结果与结论:①加味逍遥散对肝癌模型大鼠肝组织的形态结构有显著改善作用,与肝癌模型组相比,加味逍遥散组肝癌标志物Glypican-3蛋白和血清甲胎蛋白的表达均显著降低;②生物信息学分析显示,加味逍遥散组对肝癌模型组上调的miR-223-3p与基因E2F1、NCOA1存在靶向结合位点,并且与肝癌生存率及预后密切相关。由此可见,加味逍遥散对肝癌有治疗作用,可能是通过肝癌血浆来源外泌体miR-223-3p靶向负调控NCOA1/E2F1进而发挥抗肝癌效应。

关键词：肝癌加味逍遥散外泌体 miRNA 生物信息学 miR-223-3p

Effectivity and safety of trifocal intraocular lenses and capsular tension rings implantation for cataract patients with axial high myopia

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International Journal of Ophthalmology(English edition) 2024年第3期17卷 499-508页

作者： Shan Hua Qiu-xia Xie Hu Wang Jing-xiang Zhong Dongguan Aier Eye Hospital Jinan UniversityDongguan 523106Guangdong ProvinceChina Department of Ophthalmology the First Affiliated Hospital of Jinan UniversityGuangzhou 510630Guangdong ProvinceChina Department of Ophthalmology the Sixth Affiliated Hospital of Jinan UniversityDongguan 523576Guangdong ProvinceChina

●AIM:To assess effectivity and safety of trifocal intraocular lenses(IOLs)and capsular tension rings in treating cataract patients with axial high myopia.●METHODS:A prospective nonrandomized controlled clinical trial was *** 98 eyes(74 patients)who underwent femtosecond laser-assisted cataract surgery(FLACS)with trifocal IOLs were enrolled in the study and followed up for 2y after surgery:46 eyes(33 patients)with capsular tension ring implantation in the long axial lengths(AL)group(260.05).The dysfunctional lens index and total modulation transfer function(MTF)average height were similar between the two *** postoperative internal coma aberrations in the axial high myopia eyes were significantly higher than that in the normal AL group(P<0.05).The total satisfaction score in the long AL group(91.32±2.76)was slightly higher than that in the normal AL group(90.36±3.47),but there was no difference(P=0.136).A statistically negative correlation was found between corrected distance visual acuity(CDVA)and dysfunctional lens index(r=-0.382,P=0.009),and between CDVA and the total MTF average height(r=-0.374,P=0.01).But there was no significant correlation between CDVA and total satisfaction score(r=0.059,P=0.696).Postoperative complications mainly presented as posterior capsular opacity(PCO),retinal detachment and cystoid macular *** was no difference in the incidenc

关键词： trifocal intraocular lenses capsular tension ring axial high myopia cataract visual quality

Data-driven Estimation of Cloud Effects on Surface Irradiance at xianghe,a Suburban Site on the North China Plain

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Advances in Atmospheric Sciences 2022年第12期39卷 2213-2223页

作者： Mengqi LIU Jinqiang ZHANG Hongrong SHI Disong FU xiang'ao xia Key Laboratory of Atmospheric Sounding Chengdu University of Information TechnologyChengdu 610225China Key Laboratory of Middle Atmosphere and Global Environment Observation Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters Nanjing University of Information Science&TechnologyNanjing 210044China

Clouds are a dominant modulator of the energy *** cloud shortwave radiative effect at the surface(CRE)is closely related to the cloud macro-and micro-physical *** observation of surface irradiance and cloud properties are needed to narrow uncertainties in *** this study,1-min irradiance and Total Sky Imager measurements from 2005 to 2009 at xianghe in North China Plain are used to estimate cloud types,evaluate cloud fraction(CF),and quantify the sensitivities of surface irradiance with respect to changes in CF whether clouds obscure the sun or *** annual mean CF is 0.50,further noting that CF exhibits a distinct seasonal variation,with a minimum in winter(0.37)and maximum in summer(0.68).Cumulus occurs more frequently in summer(32%),which is close to the sum of the occurrence of stratus and *** annual CRE is–54.4 W m^(–2),with seasonal values ranging from^(–2)9.5 W m^(–2)in winter and–78.2 W m^(–2)in *** clouds do not obscure the sun,CF is a dominant factor affecting diffuse irradiance,which in turn affects global *** is a positive linear relationship between CF and CRE under sun-unobscured conditions,the mean sensitivity of CRE for each CF 0.1 increase is about 1.2 W m^(–2)[79.5°xianghe is used in this study,our results are representative of neighboring areas,including most parts of the North China Plain.

关键词： cloud radiative effect solar radiation cloud fraction cloud-radiation interaction

Combining QTL mapping and expression profile analysis to identify candidate genes of cold tolerance from Dongxiang common wild rice(Oryza rufipogon Griff.)

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Journal of Integrative Agriculture 2016年第9期15卷 1933-1943页

作者： ZHAO Jie QIN Jing-jing SONG Qian SUN Chuan-qing LIU Feng-xia State Key Laboratory of Plant Physiology and Biochemistry National Center for Evaluation of Agricultural Wild Plants (Rice)/Beijing Key Laboratory of Crop Genetic Improvement/Department of Plant Genetics and Breeding China Agricultural University

Rice（Oryza sativa L.）, a tropical and subtropical crop, is susceptible to low temperature stress during seedling, booting, and flowering stages, which leads to lower grain quality levels and decreasing rice yields. Cold tolerance is affected by multiple genetic factors in rice, and the complex genetic mechanisms associated with chilling stress tolerance remain unclear. Here, we detected seven quantitative trait loci（QTLs） for cold tolerance at booting stage and identified one cold tolerant line, SIL157, in an introgression line population derived from a cross between the indica variety Guichao 2, as the recipient, and Dongxiang common wild rice, as the donor. When compared with Guichao 2, SIL157 showed a stronger cold tolerance during different growth stages. Through an integrated strategy that combined QTL-mapping with expression profile analysis, six candidate genes, which were up-regulated under chilling stress at the seedling and booting developmental stages, were studied. The results may help in understanding cold tolerance mechanisms and in using beneficial alleles from wild rice to improve the cold tolerance of rice cultivars through molecular marker-assisted selection.

关键词： common wild rice introgression line low temperature tolerance different growth stages candidate genes

Antibody detection and sequence analysis of sporadic HEV in xiamen region

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World Journal of Gastroenterology 1999年第3期5卷 270-272页

作者： Huang RT Li XY xia XB Yuan XT Liu MX Li DR Acad Mil Med Sci Inst Microbiol & Epidemiol Beijing 100850 Peoples R China

INTRODUCTIONHepatitis E virus (HEV) is transmitted through a fecal-oral route［1］. HEV induces acute hepatitis and is responsible for a significant portion of the fulminant hepatitis in epidemic and sporadic cases, especially in the mixed infection patients and women in their third trimester of pregnancy［1］. It has been reported that HEV infection is more prevalent in underdeveloped and developing countries in Asia, Africa, and Central America, but is rare in developed countries［1］. In China, a large outbreak occurred between 1986 and 1988 in Xinjiang, and sporadic spread was often found in other regions.

关键词： HEV

Metabolomic and proteomic responses of Phaeodactylum tricornutum to hypoxia

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Journal of Oceanology and Limnology 2022年第5期40卷 1963-1973页

作者： Peipei ZHAO Qinghua WU Xuekui xia Shiyi GUO Sizhong SHEN Yujue WANG Aiyou HUANG Key Biosensor Laboratory of Shandong Province Biology InstituteQilu University of Technology(Shandong Academy of Sciences)Jinan 250103China State Key Laboratory of Marine Resource Utilization in the South China Sea Hainan UniversityHaikou 570228China Laboratory of Development and Utilization of Marine Microbial Resource Hainan UniversityHaikou 570228China Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province Haikou 570228China College of Marine Sciences Hainan UniversityHaikou 570228China Department of Chemistry University of WashingtonSeattle WA 98195USA

Diatoms are important contributors to global net primary productivity,and play a crucial role in the biogeochemical cycles of carbon,phosphorus,nitrogen,iron,and *** in some regions in the ocean,there’s a trend that carbon content is high while oxygen concentration is low,and the underlying mechanisms of diatoms’response to low oxygen environments are worth *** tricornutum is a model diatom whose genome has been sequenced;it provides a universal molecular toolbox and a stable transgenic expression ***,the study of the responses of *** to low oxygen has not only fundamental research significance but also important ecologic al *** this study,growth rates were determined and proteomic analysis and metabolomic analysis were performed to examine *** responses under different oxygen concentrations(2%oxygen concentration for hypoxic condition and 21%oxygen concentration for the normal condition(sterilized air)).Results show that the hypoxic environment inhibited the growth of *** the hypoxic conditions,*** could reset its metabolism pathways,including enhancement in lipid utilization,replenishment of tricarboxylic acid(TCA)cycle through the glyoxylic acid cycle,and down-regulation of photorespiration to reduce energy ***,the stress resistance mechanism was activated to facilitate the adaptation to low oxygen *** study helps to reveal the different metabolic changes to hypoxia of diatom from that of higher plants,which might be ascribed to their different habitats and needs further exploration in the future.

关键词： Phaeodactylum tricornutum hypoxia metabolomics proteomics

Cyclic Variability in Chemical Composition and Swelling-Shrinkage Properties of the xiashu Loess Stratum near Nanjing,China

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Pedosphere 2007年第5期17卷 666-672页

作者： xia Jia HUANG Guang-Long LIU Zi-Tong CHEN Xin-Min Institute of Geotechnical Engineering Nanjing University of Technology Nanjing 210009 （China）

xiashu loess strata are widely distributed along the middle-lower reaches of the Yangtze River in East China with the stratum near Nanjing,Jiangsu Province being *** cyclic variability of the stratum was investigated through comparison of geological features,chemical composition,and swelling-shrinkage properties between the loess and the paleosol *** on the investigation of such a cyclic variability,soil behavior associated with the unsaturated state of the xiashu loess stratum and its usefulness for geotechnical design and construction in the Nanjing area was analyzed. The stratum consisted of two types of cyclically alternating soil layers,namely the loess and the paleosol layers,which was the major cause for its cyclic *** loess and the paleosol layers were formed in different sedimentary *** order to avoid detrimental results from the differential engineering properties between them,this study suggested for the practitioners the importance of inspecting the variation of water content and controlling the downward net fluxes during geotechnical construction in the Nanjing area.

关键词： chemical composition cyclic variability swelling-shrinkage properties unsaturated state xiashu loessstratum

Based on non-targeted metabolomics for differential components screening of Rosae Chinensis Flos and Rosae Rugosae Flos and their quality evaluation

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Traditional Medicine Research 2025年第2期10卷 1-15页

作者： Xu Liang Ni-Hui Zhang Zhi-Lai Zhan Guang-Lu Chang Yan Gao xia Li Wen-Yuan Gao School of Pharmaceutical Science and Technology Faculty of MedicineTianjin UniversityTianjin 300193China State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs China Academy of Chinese Medical SciencesBeijing 100700China Key Laboratory of Modern Chinese Medicine Resources Research Enterprises Tianjin 300402China

Background:Rosa chinensis *** Rosa rugosa *** not only of ornamental value,but also edible flowers and the flower buds have been listed in the Chinese Pharmacopoeia as traditional *** two plants have some differences in efficacy,but the flower buds are easily confused for similar *** addition,large-scale cultivation of ornamental rose flowers may lead to a decrease in the effective components of medicinal ***,it is necessary to study the chemical composition and make quality evaluation of Rosae Chinensis Flos(Yueji)and Rosae Rugosae Flos(Meigui).Methods:In this study,40 batches of samples including Meigui and Yueji from different regions in China were collected to establish high-performance liquid chromatography ***,the fingerprints data was analyzed using principal component analysis,hierarchical cluster analysis,and partial least squares discriminant analysis analysis chemometrics to obtain information on intergroup differences,and non-targeted metabolomic techniques were applied to identify and compare chemical compositions of samples which were chosen from groups with large *** compounds were screened by orthogonal partial least-squares discriminant analysis and S-plot,and finally multi-component quantification was performed to comprehensively evaluate the quality of Yueji and ***:The similarity between the fingerprints of 40 batches roses and the reference print R was 0.73 to 0.93,indicating that there were similarities and differences between the *** principal component analysis and hierarchical cluster analysis of fingerprints data,the samples from different origins and varieties were intuitively divided into four *** least-squares discriminant analysis analysis showed that Meigui and Yueji cluster into two categories and the model was reliable.A total of 89 compounds were identified by high resolution mass spectrometry,mainly were flavonoids and flavon

关键词： Rosa chinensis Jacq. Rosa rugosa Thunb. metabolomics chemometrics multiple component quantification quality evaluation

Influence of axial stress on rockburst in deep tunnels: insight from model experiment

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Journal of Mountain Science 2024年第10期21卷 3536-3551页

作者： YAN Yaofeng xia Yuanyou HUANG Jian LIN Manqing LIU Xiqi YAN Minjia ZHANG Hongwei School of Civil Engineering and Architecture Wuhan University of TechnologyWuhan 430070China School of Resources and Safety Engineering Wuhan Institute of TechnologyWuhan 430074China Key Laboratory of Geotechnical and Structural Engineering Safety of Hubei Province School of Civil EngineeringWuhan UniversityWuhan 430000China China Railway 11th Bureau Group Fourth Engineering Co. Ltd.Wuhan 430074China School of Resource and Environment Sciences Wuhan UniversityWuhan 430000China

Frequent rockburst disasters in deep-buried engineering projects severely impact construction. To explore the influence of axial stress on rockburst in deep-buried tunnels, large-scale true triaxial rockburst experiments were conducted under four different axial stress ratio conditions (ηt, axial loading stress/vertical loading stress) using a self-developed true triaxial loading device under the condition of "pre-loading before excavation". The influence of axial stress on the rockburst process and failure characteristics in deep tunnels was studied using a combination of real-time video monitoring, rockburst debris sieving, and acoustic emission monitoring. The results indicate: (1) all four specimens subjected to different axial stress ratio loading conditions exhibited three stages of macroscopic failure: small particle ejection, flake spalling, and large fragment ejection. Ultimately, "V"-shaped notches appeared on both sides of the tunnel. (2) The failure stress, fragment volume, and fragment size distribution of the rockburst specimens exhibited a clear two-stage failure characteristic with increasing axial stress ratio. In the lower axial stress ratio stage (ηt ≤ 0.7), the increase in the axial stress ratio enhances lateral confinement, thereby increasing the crack initiation strength of the surrounding rock, inhibiting crack formation and propagation, and thus suppressing damage to the surrounding rock of the tunnel. In the higher axial stress ratio stage (ηt > 0.7), the increase in axial stress ratio makes the Poisson effect of the surrounding rock more pronounced, promoting the generation and propagation of cracks along the tunnel axis direction, thereby promoting damage to the surrounding rock. (3) Based on the analysis of acoustic emission parameters (fracture properties), it can be concluded that in the lower axial stress ratio stage (ηt ≤ 0.7), an increase in the axial stress ratio leads to a higher proportion of shear fracture in rockburst damage. Co

关键词： Rockburst experiments Axial stress ratio Failure characteristics Fracture properties