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Phyton-International Journal of Experimental Botany 2021年第1期90卷 99-110页

作者： Siyi Fu Tao Yun Dexuan Ma Bingsong Zheng Dean Jiang Yi he State Key Laboratory of Subtropical Silviculture Zhejiang A&F UniversityHangzhou311300China china Science and Technology Exchange center Beijing100045China State Key Laboratory of Plant Physiology and Biochemistry College of Life SciencesZhejiang UniversityHangzhou310058China

The chloroplast NAD(P)H dehydrogenase(NDH)complex,as one of the most important photosynthesis protein complexes in thylakoid membrane,is involved in photosystem I(PSI)cyclic electron transport(cEF).Under abiotic environmental stress,the photosynthetic apparatus is susceptible to the damage caused by the strong light ***,the enhancement of NDHdependent cEF could facilitate the alleviation of the damage to the photosynthetic *** NdhB subunit encoded by chloroplast genome is one of most important subunits of NDH complex and consists of 510 amino ***,according to cloning ndhB from Melrose(cultivated soybean),Acc547(wild salt-tolerant soybean),S113-6 and S111-9(hybrid descendant),based on the comparison and analysis of the sequences of NdhB subunits,we found that there is a novel thylakoid transit peptide of NdhB subunit in *** addition,crosslink immunoprecipitation,immunogold labeling and co-expression of GFP fusion protein indicated that the novel thylakoid transit peptide is favorable to the expression and localization of NdhB subunit in ***,we suggest that this novel thylakoid transit peptide plays the same role as chaperonin and contributes to facilitating the expression and localization of NdhB subunit.

关键词： NDH complex NdhB subunit thylakoid transit peptide

甲型H1N1流感病毒感染致病候选基因的鉴定

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中华医院感染学杂志 2024年第4期34卷 481-488页

作者：刘慧焦芹芹贺宏杰韩哲袁牡丹张辉徐福意董浩胜利油田中心医院儿科呼吸感染科山东东营2570342 胜利油田中心医院感染管理部山东东营257034 滨州医学院药学院山东烟台264000 包头市肿瘤医院放疗科内蒙古包头014030 包头市肿瘤医院放射科内蒙古包头014030

目的通过c57BL/6J(B6)和DBA/2J(D2)亲本品系小鼠流感感染后相关表型及杂交子代BxD小鼠肺脏转录本数据的系统性分析进行基因筛选,以找到在流感病毒感染中发挥作用的候选基因.方法共选取41个BxD小鼠及亲本品系B6和D2的雌性小鼠,在8~12周... 详细信息

目的通过c57BL/6J(B6)和DBA/2J(D2)亲本品系小鼠流感感染后相关表型及杂交子代BxD小鼠肺脏转录本数据的系统性分析进行基因筛选,以找到在流感病毒感染中发挥作用的候选基因.方法共选取41个BxD小鼠及亲本品系B6和D2的雌性小鼠,在8~12周龄之间给予流感病毒甲型鼠肺适应株(H1N1,PR8M)得到相关表型及基因表达量数据,对基因表达量进行聚类分析及加权基因共表达网络分析,构建模块特征值与小鼠病毒载量的相关性,经表达数量性状座位分析,皮尔逊相关性分析,结合全基因组关联研究(GWAS)、小鼠基因组信息学(MGI)、国际小鼠表型联合会(IMPc)三个基因数据库中的信息分析筛选流感病毒感染相关的候选功能基因及确定该基因的遗传调控方式;最后将挑选出的基因进行通路富集分析.结果通过聚类分析和加权基因共表达网络分析,将24219个基因分为23个模块;通过模块与表型的相关性分析,发现MEred、MEgreen、MEma-genta模块与感染密切相关,将其作为核心模块;对核心模块基因通过KEGG和MPO富集分析,结果显示流感病毒相关基因显著参与MAPK信号通路、TNF信号通路等多个细胞免疫相关通路;对核心模块内基因与BxD小鼠流感病毒感染的3种表型(体质量减轻,平均死亡时间和感染后体质量减轻中位数)进行相关性分析,共发现21个基因的肺mRNA表达水平与3种表型均相关(Pcpl2受顺式遗传调控,Dusp12、Ovol2、catsper4、Tmc5、Acsm 1、Fam81a、Rtn4、Rhpn1受反式遗传调控;通过结合MGI,IMPc,GWAS三个基因数据库中的信息分析发现部分基因在免疫通路方面发挥作用.结论通过系统遗传学分析,在小鼠基因组上鉴定了影响流感病毒感染相关的21个候选基因,后续可做进一步的功能验证来阐明其参与流感病毒感染的遗传调控机制.

关键词：流感病毒 BxD小鼠 Reg3g 候选基因

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花粉发酵研究进展

保鲜与加工 2024年第7期24卷 117-123页

作者：洪佳瑞张丽梅王思凡贺韦衡王玥唐宁程永强中国农业大学食品科学与营养工程学院植物源功能食品北京市重点实验室北京100083 国珍健康科技有限公司北京102206 泉州师范学院海洋与食品学院福建泉州362000

花粉为植物雄性配子体,营养组成全面均衡,活性物质含量较高,是优良的保健食品原料。对花粉的营养组成、保健功能及花粉发酵进行概述,着重阐述了花粉固态及液态发酵的方式和作用,以期为花粉产品深加工提供可行的研究思路及科学依据,推动... 详细信息

花粉为植物雄性配子体,营养组成全面均衡,活性物质含量较高,是优良的保健食品原料。对花粉的营养组成、保健功能及花粉发酵进行概述,着重阐述了花粉固态及液态发酵的方式和作用,以期为花粉产品深加工提供可行的研究思路及科学依据,推动我国花粉市场进一步发展。

关键词：花粉营养组成保健功能发酵

基于增强cT影像组学及基因组学模型预测卵巢浆液性囊腺癌预后

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中国医学影像技术 2024年第5期40卷 745-751页

作者：何迪梁赵建新潘妮妮施柳言熊恋秋马丽丽赵致平赵莲萍黄刚甘肃中医药大学第一临床医学院甘肃兰州730000 甘肃省武威肿瘤医院放射科甘肃武威733000 甘肃省人民医院放射科甘肃兰州730000

目的探讨基于治疗增强前动脉期cT构建的影像组学模型和影像-基因组学模型预测卵巢浆液性囊腺癌(OSc)患者预后的价值。方法回顾性收集2个中心及癌症影像档案馆(TcIA)共110例OSc增强动脉期cT图像,提取其影像组学特征,建立影像组学cox回归... 详细信息

目的探讨基于治疗增强前动脉期cT构建的影像组学模型和影像-基因组学模型预测卵巢浆液性囊腺癌(OSc)患者预后的价值。方法回顾性收集2个中心及癌症影像档案馆(TcIA)共110例OSc增强动脉期cT图像,提取其影像组学特征,建立影像组学cox回归预后预测模型;于癌症基因组图谱(TcGA)数据库获取399例OSc(TcGA-OV)转录组数据,以Pearson相关系数筛选与预测模型纳入特征相关的基因,进行关联基因富集分析。以cox回归及蛋白质互作网络(PPI)对57例同时具有完整影像学及转录组学资料的OSc患者(TcGA-TcGA-OV)筛选连接度最高的预后枢纽基因,建立影像-基因组学模型。观察上述模型预测OSc预后的效能。结果基于5个OSc预后相关影像组学特征的建立影像组学模型在相应训练集和测试集的c指数(c-index)分别为0.782和0.735;纳入30个预后枢纽基因建立的影像-基因组学模型在相应训练集和测试集的c-index分别为0.673和0.659。以上述模型分层后,不同层次患者间生存率差异有统计学意义(P均c预后;分析OSc mRNA生物信息学可为影像组学模型提供生物学可解释性。

关键词：卵巢囊腺癌,浆液预后影像基因组学体层摄影术,x线计算机

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comparison of clinical efficacy and postoperative inflammatory response between laparoscopic and open radical resection of colorectal cancer

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World Journal of clinical cases 2022年第13期10卷 4042-4049页

作者： Long-Hai he Bo Yang xiao-Qin Su Yue Zhou Zhen Zhang Department of General Surgery The First People’s Hospital of Wanzhou DistrictChongqing 404040China Department of clinical Nutrition The First People’s Hospital of Wanzhou DistrictChongqing 404040China

BAcKGROUND In recent years,the incidence of colorectal cancer(cRc)has increased annually,which has seriously threatened the health and quality of life of *** the treatment of cRc,both laparoscopic and radical resection are widely *** To explore and discuss clinical efficacy and postoperative inflammatory response of laparoscopic and open radical resection of *** A total of 96 patients with cRc diagnosed in our hospital from March 2016 to April 2021 were selected,and were divided into the study group(n=48)and control group(n=48)using a simple random *** control group was treated with open radical resection of cRc,and the study group was treated with laparoscopic radical resection of *** perioperative conditions(operation time,intraoperative blood loss,the recovery time of gastrointestinal function,number of lymph node dissections and length of hospital stay),inflammatory response index levels[interleukin(IL)-6,IL-8,IL-10,c-reactive protein(cRP)]before and after operation,pain stress response indices[levels of neuropeptide(NPY),prostaglandin E2(PGE2),5-hydroxytryptamine(5-HT)],and the incidence of the complications between the two groups were *** The operation time in the study group was(186.18±33.54 min),which was longer than that of the control group(129.38±26.83 min),but the intraoperative blood loss(111.34±21.45 mL),recovery time of gastrointestinal function(25.35±4.55 h),and hospital stay(10.09±2.38 d)were better than those in the control group(163.77±32.41 mL,36.06±7.13 h,13.51±3.66 d)(Phere was no significant difference in the number of lymph node dissections between the study group(15.19±3.04)and the control group(16.20±2.98)(P>0.05).There was no significant difference between the levels of serum IL-6(9.79±4.11 ng/mL),IL-8(3.79±1.71 ng/L),IL-10(48.96±12.51 ng/L)and cRP(7.98±2.33 mg/L)in the study group and the control group(10.56±3.78 ng/mL,4.08±1.45 ng/L,50.13±11.67 ng/L,8.29±2.60 mg/L)before the operation(P

关键词： Laparoscope Open surgery colorectal cancer Inflammatory response Pain stress response

Radiomics-based predictive risk score: A scoring system for preoperatively predicting risk of lymph node metastasis in patients with resectable non-small cell lung cancer

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chinese Journal of cancer Research 2019年第4期31卷 641-652页

作者： Lan he Yanqi Huang Lixu Yan Junhui Zheng changhong Liang Zaiyi Liu Department of Radiology Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhou 510080China Department of Pathology Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhou 510080China

Objective: To develop and validate a radiomics-based predictive risk score(RPRS) for preoperative prediction of lymph node(LN) metastasis in patients with resectable non-small cell lung cancer(NScLc).Methods: We retrospectively analyzed 717 who underwent surgical resection for primary NScLc with systematic mediastinal lymphadenectomy from October 2007 to July 2016. By using the method of radiomics analysis, 591 computed tomography(cT)-based radiomics features were extracted, and the radiomics-based classifier was constructed. Then, using multivariable logistic regression analysis, a weighted score RPRS was derived to identify LN metastasis. Apparent prediction performance of RPRS was assessed with its calibration,discrimination, and clinical ***: The radiomics-based classifier was constructed, which consisted of 13 selected radiomics *** models demonstrated that radiomics-based classifier, age group, tumor diameter, tumor location, and cT-based LN status were independent predictors. When we assigned the corresponding score to each variable,patients with RPRSs of 0-3, 4-5, 6, 7-8, and 9 had distinctly very low(0%-20%), low(21%-40%), intermediate(41%-60%), high(61%-80%), and very high(81%-100%) risks of LN involvement, respectively. The developed RPRS showed good discrimination and satisfactory calibration (c-index: 0.785, 95% confidence interval(95% cI):0.780-0.790)Additionally, RPRS outperformed the clinicopathologic-based characteristics model with net reclassification index(NRI) of 0.711(95% cI: 0.555-0.867).conclusions: The novel clinical scoring system developed as RPRS can serve as an easy-to-use tool to facilitate the preoperatively individualized prediction of LN metastasis in patients with resectable NScLc. This stratification of patients according to their LN status may provide a basis for individualized treatment.

关键词： Lymph node radiomics risk score cT non-small cell lung cancer

Is the metal involved or not? A computational study of cu(I)-catalyzed [4+1] annulation of vinyl indole and carbene precursor

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chinese chemical Letters 2022年第4期33卷 2031-2035页

作者： xiaoqian he Kangbao Zhong Dan heng Zhen Zeng Hao Ni Ruopeng Bai Yu Lan School of chemistry and chemical Engineering Chongqing Key Laboratory of Theoretical and Computational ChemistryChongqing UniversityChongqing 400030China college of chemistry and Institute of Green catalysis Zhengzhou UniversityZhengzhou 450001China

The cu(I)-catalyzed [4 + 1] annulation of vinyl indoles and a carbene precursor is a powerful method for constructing cyclopentaindole derivatives. Density functional theory(DFT) calculations were used to elucidate the mechanism and regioselectivity of this reaction. After cu-assisted indole c3-alkylation, direct1,5-annulation was favored over the cu-assisted annulation pathway. Furthermore, the regioselectivity for1,5-annulation was attributed to the generated five-membered-ring product being more stable than the three-membered-ring product from 1,3-annulation, which was the kinetically favored pathway.

关键词： carbene precursor Regioselectivity DFT calculations cyclopentaindol c3-alkylation

PREPARATION OF Fe/Ni MULTILAYER FILMS AND TheIR MAGNETIc PROPERTIES

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Acta Metallurgica Sinica(English Letters) 2002年第2期15卷 182-186页

作者： L.L. Liu, x.F. Bi, S.K. Gong, H.B. xu and x. Y. Yuan Department of Materials Science and Engineering, Beijing University of Aeronautics and Astronautics, Beijing 100083, china Department of Materials Science and Engineering Beijing University of Aeronautics and Astronautics

FeNi multilayer films were prepared by EB-PVD technique. A modified TG-DSc apparatus was used to measure their curie temperatures, which were around 7600c, lower than that of pure Fe thin films. The easy magnetization axis was in-plane. High temperature annealing in vacuum decreased the coercivity sharply. The saturation magnetization also changed with heat treatment. After annealing at the temperature equal to the substrate temperature during deposition, the saturation magnetization decreased.

关键词： multilayer film EB-PVD. curie transition point saturation magnetization. coercivity

桂西北喀斯特地区退化群落灌草不同器官N、P生态化学计量内稳性特征

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生态学报 2024年第12期44卷 5367-5376页

作者：俞月凤韦建华胡钧铭张俊辉李婷婷郑富海张野苏利荣何铁光广西壮族自治区农业科学院农业资源与环境研究所/广西耕地保育重点实验室南宁530007 广西林业勘测设计院南宁530011 不详

植物的生态化学计量内稳性特征是长期适应环境的结果,与其生态适应性策略有关。探明喀斯特退化群落灌草不同器官N、P元素的化学计量特征及其内稳性特征,为喀斯特地区植被群落退化过程中植物的适应策略研究提供依据,基于桂西北喀斯特地区... 详细信息

植物的生态化学计量内稳性特征是长期适应环境的结果,与其生态适应性策略有关。探明喀斯特退化群落灌草不同器官N、P元素的化学计量特征及其内稳性特征,为喀斯特地区植被群落退化过程中植物的适应策略研究提供依据,基于桂西北喀斯特地区5种退化程度植被群落的调查取样,测定不同退化群落灌木(叶、茎和根)和草本(地上部分和地下部分)N、P含量,分析其化学计量特征、不同器官间的相互关系及其与土壤理化性质的相关性以及内稳性特征。结果表明:(1)灌木不同器官P含量表现为叶>茎>根,N含量和N∶P表现为叶>根>茎,草本P、N含量及N∶P均为地上部分>地下部分;随退化程度加剧,灌木各器官和草本地下部分的N、P含量及灌木根N∶P均呈降低变化趋势,草本地上部分的N、P含量在前四个退化阶段为降低趋势,在Ⅴ阶段有所升高,草本地下部分N∶P则呈现先升高后降低趋势,其他N∶P无显著性变化;灌草叶片N∶P均小于14;(2)N和P含量在各器官中均呈显著正相关关系;灌草的不同器官之间的同一营养元素及元素比,以及灌木和草本之间的各器官的同一营养元素及元素比均呈正相关关系;(3)灌草各器官N、P含量、N∶P与土壤TN、TP、N∶P存在显著的正相关关系,灌草各器官P含量与土壤c∶P显著负相关;(4)灌木各器官H_(N)、Hp和H_(N∶P)均表现为叶>茎>根,草本地上部分的H_(N)大于地下部分,Hp和H_(N∶P)则反之;灌草同种器官不同元素之间则表现为N>P>N∶P。喀斯特地区退化群落中灌木较草本具有更高的内稳性,灌草生长主要受N限制,且N元素表现较高的内稳性;灌草各器官在植被群落退化过程中对N、P含量变化的响应具有一致性和协同性。

关键词：内稳性生态化学计量学植物器官喀斯特退化群落