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Viral and nonviral nanocarriers for in vivo CRISPR-based gene editing

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Nano Research 2024年第10期17卷 8904-8925页

作者： Zhongyuan Guo Audrey t.Zhu Ronnie H.fang Liangfang Zhang Aiiso Yufeng Li Family Department of Chemical and Nano Engineering University of California San DiegoLa JollaCA 92093USA Division of Host-Microbe Systems and therapeutics Department of PediatricsUniversity of California San DiegoLa JollaCA 92093USA

the continued development of clustered regularly interspaced short palindromic repeats(CRISPR)technology has the potential to greatly impact clinical medicine,particularly for disease diagnosis and *** high demand for the in vivo delivery of CRISPR-based therapies,significant challenges *** include rapid degradation by enzymes,inefficient disease site targeting,and the risk of undesired off-target *** platforms,with their tailorable properties,have been engineered to efficiently package CRISPR payloads in various formats,including as plasmid DNA,mRNA,and ribonucleoprotein complexes,for in vivo *** them,recombinant adeno-associated viruses,virus-like particles,and lipid nanoparticles have displayed exceptional *** review will discuss the development of these and other nanocarriers for in vivo CRISPR-based genome editing.

关键词： clustered regularly interspaced short palindromic repeats(CRISPR) in vivo gene editing nanocarriers recombinant adeno-associated viruses(rAAVs) virus-like particle(VLP) lipid nanoparticle(LNP)

Fe-N-C core-shell catalysts with single low-spin Fe(Ⅱ)-N_(4)species for oxygen reduction reaction and high-performance proton exchange membrane fuel cells

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Journal of Energy Chemistry 2024年第6期93卷 538-546,I0013页

作者： Yan Wan Linhui Yu Bingxin Yang Caihong Li Chen fang Wei Guo fang-xing xiao Yangming Lin xiamen Key Laboratory of Rare Earth Photoelectric Functional Materials Xiamen Institute of Rare Earth MaterialsHaixi InstituteChinese Academy of SciencesXiamen 361021FujianChina CAS Key Laboratory of Urban Pollutant Conversion Institute of Urban EnvironmentChinese Academy of SciencesXiamen 361021FujianChina Fujian Institute of Research on the Structure of Matter Chinese Academy of SciencesFuzhou 350002FujianChina College of Materials Science and Engineering Fuzhou UniversityNew CampusMinhou 350108FujianChina College of Chemistry and Materials Science Fujian Normal UniversityFuzhou 350117FujianChina

Fe-N-doped carbon materials(Fe-N-C)are promising candidates for oxygen reduction reaction(ORR)relative to Pt-based catalysts in proton exchange membrane fuel cells(PEMFCs).However,the intrinsic contributions of Fe-N_(4)moiety with different chemical/spin states(e.g.D1,D2,D3)to ORR are unclear since various states coexist *** the present work,Fe-N-C core-shell nanocatalyst with single lowspin Fe(Ⅱ)-N_(4)species(D1)is synthesized and identified with ex-situ ultralow temperature Mossbauer spectroscopy(t=1.6 K)that could essentially differentiate various Fe-N_(4)states and invisible Fe-O *** quantifying with CO-pulse chemisorption,site density and turnover frequency of Fe-N-C catalysts reach 2.4×10^(-9)site g^(-1)and 23 e site~(-1)s^(-1)during the ORR,***-wave potential(0.915V_(RHE))of the Fe-N-C catalyst is more positive(approximately 54 mV)than that of Pt/***,we observe that the performance of PEMFCs on Fe-N-C almost achieves the 2025 target of the US Department of Energy by demonstrating a current density of 1.037 A cm^(-2)combined with the peak power density of 0,685 W cm^(-2),suggesting the critical role of Fe(Ⅱ)-N_(4)site(D1).After 500 h of running,PEMFCs still deliver a power density of 1.26 W cm^(-2)at 1.0 bar H_(2)-O_(2),An unexpected rate-determining step is figured out by isotopic labelling experiment and theoretical *** work not only offers valuable insights regarding the intrinsic contribution of Fe-N_(4)with a single spin state to alkaline/acidic ORR,but also provides great opportunities for developing high-performance stable PEMFCs.

关键词： Fuel cells Oxygen reduction reaction Non-platinum group metals(PGMs) Isotopic labelling Active site tOF

Improved performance of LiMn_(0.8)Fe_(0.2)PO_(4) by addition of fluoroethylene carbonate electrolyte additive

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Chinese Chemical Letters 2024年第6期35卷 229-233页

作者： Guihuang fang Wei Chen Hongwei Yang Haisheng fang Chuang Yu Maoxiang Wu Faculty of Metallurgy and Energy Engineering Kunming University of Science and TechnologyKunming 650093China Key Laboratory of Optoelectronic Materials Chemistry and Physics Fujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhou 350002China State Key Laboratory of Advanced Electromagnetic Engineering and technology School of Electrical and Electronic EngineeringHuazhong University of Science and TechnologyWuhan 430074China

the addition of electrolyte additives is an effective strategy for tuning the property of the electrolyte to engineer the electrode/electrolyte interface,and there exist obvious discrepancies regarding the effect of fluoroethylene carbonate(FEC)as an electrolyte additive on the performance of *** FEC is introduced into the electrolyte of the LiMn_(0.8)Fe_(0.2)PO_(4)/Li cell and its effect on the properties of the LiMn_(0.8)Fe_(0.2)PO_(4) is *** is found that the addition of FEC in the electrolyte has a positive effect on the performance of the LiMn_(0.8)Fe_(0.2)PO_(4) cathode,which can be attributed to the reduced products generated by the interfacial side-reactions on the LiMn_(0.8)Fe_(0.2)PO_(4) cathode surface and the decreased metal dissolution in the FEC-containing electrolyte,thanks to the higher oxidation resistance of FEC and the easier and stronger binding of FEC and PF_6^(-).

关键词： Lithium-ion batteries LiMn_(0.8)Fe_(0.2)PO_(4) Cathode Electrolyte additives Fluoroethylene carbonate

Probing the interaction between asphaltene-wax and its effects on the crystallization behavior of waxes in heavy oil via molecular dynamics simulation

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Petroleum Science 2024年第4期21卷 2839-2848页

作者： Yong Hu xi Lu Hai-Bo Wang Ji-Chao fang Yi-Ning Wu Jian fang Sun SINOPEC Petroleum Exploration and Production Research Institute Shandong Key Laboratory of Oilfield Chemistry School of Petroleum Engineering China University of Petroleum (East China)

High content of asphaltenes and waxes leads to the high pour point and the poor flowability of heavy oil,which is adverse to its efficient development and its transportation in *** the interaction mechanism between asphaltene-wax is crucial to solve these problems,but it is still *** this paper,molecular dynamics simulation was used to investigate the interaction between asphaltenewax and its effects on the crystallization behavior of waxes in heavy *** show that molecules in pure wax are arranged in a paralleled *** wax molecules in heavy oil,which are close to the surface of asphaltene aggregates,are bent and arranged *** the mass fraction of asphaltenes in asphaltene-wax system(ωasp) is 0-25 wt%,the attraction among wax molecules decreases and the bend degree of wax molecules increases with the increase of ω*** ωaspincreases from 0 to 25 wt%,and the attraction between asphaltene-wax is stronger than that among *** causes that the wax precipitation point changes from 353 to 333 *** the ωaspincreases to 50 wt%,wax molecules are more dispersed owing to the steric hindrance of asphaltene aggregates,and the interaction among wax molecules transforms from attraction to *** causes that the ordered crystal structure of waxes can't be formed at normal ***,the asphaltene,with the higher molecular weight or the more hetero atoms,has more obvious inhibition to the formation of wax ***,resins also have an obvious inhibition on the wax crystal due to the formation of asphalteneresin aggregates with a larger *** results reveal the interaction mechanism between asphaltene-wax,and provide useful guidelines for the development of heavy oil.

关键词： Heavy oil Interaction mechanism Asphaltenes Waxes Molecular dynamics

Navigating biomolecular condensates in plants from patterns to functions

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Molecular Plant 2024年第9期17卷 1329-1332页

作者： Yansong Miao Monika Chodasiewicz xiaofeng fang School of Biological Sciences Nanyang Technological UniversitySingapore 637551Singapore institute for Digital Molecular Analytics and Science Nanyang Technological UniversitySingapore636921Singapore Biological and Environmental Science&Engineering Division(BESE) King Abdullah University of Science and Technology(KAUST)ThuwalSaudi Arabia School of Life Sciences Tsinghua UniversityBeijingChina

Biomolecular condensates have emerged as one of the important focuses of recent research on plant *** study of these condensates often begins with evidence gathered from imaging or bioinformatics *** with genetic and biochemical approaches,researchers have begun to establish a link between the condensation of biomolecules *** challenge,however,is unambiguously demonstrating the necessity of condensation in a cellular process versus the macromolecule *** requires a combination of evidence from genetics,cell biology,biochemistry,and biophysical *** this Opinion paper,we pinpoint the factors to be considered when studying the role of biomolecular condensates in plant *** also provide future directions for plant condensate biology.

关键词： functions. condensation directions

Beyond network centrality: individual-level behavioral traits for predicting information superspreaders in social media

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National Science Review 2024年第7期11卷 148-157页

作者： fang Zhou Linyuan Lü Jianguo Liu Manuel Sebastian Mariani Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of China Yangtze Delta Region Institute(Huzhou) University of Electronic Science and Technology of China School of Cyber Science and technology University of Science and Technology of China Department of Digital Economics Shanghai University of Finance and Economics URPP Social Networks Universit?t Zürich

Understanding the heterogeneous role of individuals in large-scale information spreading is essential to manage online behavior as well as its potential offline consequences. to this end, most existing studies from diverse research domains focus on the disproportionate role played by highly connected ‘hub’ ***, we demonstrate here that information superspreaders in online social media are best understood and predicted by simultaneously considering two individual-level behavioral traits: influence and susceptibility. Specifically, we derive a nonlinear network-based algorithm to quantify individuals' influence and susceptibility from multiple spreading event data. By applying the algorithm to large-scale data from twitter and Weibo, we demonstrate that individuals' estimated influence and susceptibility scores enable predictions of future superspreaders above and beyond network centrality, and reveal new insights into the network positions of the superspreaders.

关键词： complex networks social networks social media information spreading superspreaders

Sequence-Controlled Synthesis of Alternating Poly(ester amide)s using Water as Control Agent in One-Pot

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Chinese Journal of Polymer Science 2024年第8期42卷 1015-1020页

作者： tao Liu fang-fang Niu Kan Cheng Yu-Min xia Bo-Mou Ma xue-Li Wang Jian-Yong Yu Yong He State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and EngineeringDonghua UniversityShanghai 201620China Innovation Center for textile Science and technology Donghua UniversityShanghai 201620China

Poly(ester amide)s(PEAs)attract much attention as a new kind of biodegradable ***,the synthesis of PEAs with sequence-regulated chain structures is still complicated due to the multistep polymerization,high monomer purity,as well as the usage of organic solvents,which greatly inhibits its development and ***,a one-pot strategy without solvent was developed to synthesize the alternating PEA fromα,ω-amino alcohol and dicarboxylic acid by sequential polycondensation,where water was used as the controlling agent for amidation and ***,the amidation and esterification were controlled to proceed in turn by adding or removing water,realizing the one-pot preparation of alternating *** resultant PEA is characterized by^1H-and^(13)C-NMR,and its chain structure is confirmed to be highly quasi-alternating similar to alternating PEAs prepared by classical methods the evaluations from DSC and DMA demonstrate that the properties of quasi-alternating PEA(t_(m)=102℃,ΔH_(m)=30J·g^(-1))are far superior to those of random PEA(t_(m)=72℃,ΔH_(m)<1 J·g^(-1)).

关键词： Biodegradable macromolecules Polymer architectures Sequence-controlled synthesis One-pot polymerization

Synchronous deprotonation–protonation for mechanically robust chitin/aramid nanofibers conductive aerogel with excellent pressure sensing, thermal management, and electromagnetic interference shielding

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Nano Research 2024年第3期17卷 2038-2049页

作者： xinxin Zhang Kunpeng Qian Jianhui fang Sineenat thaiboonrod Miao Miao xin Feng Department of Chemistry College of SciencesShanghai UniversityShanghai 200444China Research Center of Nano Science and technology College of SciencesShanghai UniversityShanghai 200444China School of Materials Sciences and Engineering Shanghai UniversityShanghai 200444China National Nanotechnology Center(NANOtEC) National Science and Technology Development Agency(NSTDA)Thailand Science ParkPathum Thani 12120Thailand

Aerogels with regularly porous structure and uniformly distributed conductive networks have received extensive attention in wearable electronic sensors,electromagnetic shielding,and so ***,the poor mechanical properties of the emerging nanofibers-based aerogels are limited in practical *** this work,we developed a synchronous deprotonation–protonation method in the KOH/dimethyl sulfoxide(DMSO)system at room temperature for achieving chitin cross-linked aramid nanofibers(CANFs)rather than chitin nanofibers(ChNFs)and aramid nanofibers(ANFs)separately by using chitin and aramid pulp as raw *** freeze-drying process,the cross-linked chitin/aramid nanofibers(CA)aerogel exhibited the synergetic properties of ChNF and ANF by the dual-nanofiber compensation *** mechanical stress of CA aerogel was 170 kPa at 80%compressive strain,increased by 750%compared with pure ChNF ***,the compressibility of CA aerogel was somewhat improved compared to ANF *** enhancement verified that the crosslinking reaction between ANF and ChNF during the synchronous deprotonation process was ***,the conductive aerogels with uniform porous structure(CA-M)were successfully obtained by vacuum impregnating CA aerogels in ti_(3)C_(2)t_(x) Mxene solution,displaying low thermal conductivity(0.01 W/(m·K)),high electromagnetic interference(EMI)shielding effectiveness(SE)(75 dB),flame retardant,and heat ***,the as-obtained CA-M aerogels were also applied as a pressure sensor with excellent compression cycle stability and superior human motion monitoring *** a result,the dual-nanofiber based conductive aerogels have great potentials in flexible/wearable electronics,EMI shielding,flame retardant,and heat insulation.

关键词： synchronous deprotonation–protonation electromagnetic interference(EMI)shielding flame retardant heat insulation pressure sensing

Deformation mechanism and limit support pressure of cutting steel plate during connection between pipes in large spacing using pipe curtain structure method

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Underground Space 2024年第5期18卷 83-96页

作者： xiang Liu Rui Zhang Jun Huang Guang Zhao Qian fang Annan Jiang Liaoning Key Laboratory of Marine Environmental Bridge and tunnel Engineering Dalian Maritime UniversityDalian 116026China JStI Group Nanjing 210017China Key Laboratory for Urban Underground Engineering of Ministry of Education Beijing Jiaotong UniversityBeijing 100044China

the pipe curtain structure method(PSM)is a novel construction method to control ground deformation *** with the traditional pipe-roofing and pipe jacking method,the connection between pipes in large spacings using PSM is widely acknowledged as a unique construction *** study on this connection procedure is needed to resolve similar cases in that the pipes are inevitably constructed on both sides of existing *** the steel plate during the connection procedure is the first step,which is crucial to control the safety and stability of the surrounding environment and existing *** deformation mechanism and limit support pressure of the cutting steel plate during the connection between pipes in large spacings are studied in this paper,relying on the undercrossing Yifeng gate tower project of Jianning West Road River Crossing Channel in Nanjing,China.A modified 3D wedge-prism failure model is proposed using the 3D discrete element *** with terzaghi loose earth pressure theory and the limit equilibrium theory,the analytical solutions for the limit support pressure of the excavation face of the cutting steel plate are *** modified 3D wedge-prism failure model and corresponding analytical solutions are categorised into two cases:(a)unilateral cutting scheme,and(b)bilateral cutting *** analytical solutions for the two cases are verified from the numerical simulation and in-situ data and compared with the previous *** comparative analysis between the unilateral and bilateral cutting schemes indicates that the bilateral cutting scheme can be adopted as a *** bilateral cutting scheme saves more time and induces less ground deformation than the unilateral one due to the resistance generated from the superimposed *** addition,the parametric sensitivity analysis is carried out using an orthogonal experimental *** main influencing factors arranged from high to low are the pipe sp

关键词： Pipe curtain structure method Wedge-prism failure model Limit support pressure Discrete element method Connection between pipes