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Isolation and Expression Analysis of fTZ-f1 Encoding Gene of Black Rock fish (Sebastes schlegelii)

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Journal of Ocean University of China 2013年第1期12卷 183-189页

作者： Muhammad Shafi WANG Yanan ZHOU xiaosu MA Liman faiz Muhammad QI Jie zhang Quanqi College of Marine Life Sciences Key Laboratory of Marine Genetics and Breeding of Ministry of Education of China Ocean University of China

Sex related fTZ-f1 is a transcriptional factor regulating the expression of fushi tarazu (a member of the orphan nuclear receptors) gene. In this study, fTZ-f1 gene (fTZ-f1) was isolated from the testis of black rockfish (Sebastes schlegeli) by homology cloning. The full-length cDNA of S. schlegeli fTZ-f1 (ssfTZ-f1) contained a 232bp 5′UTR, a 1449bp ORf encoding fTZ-f1 (482 amino acid residules in length) with an estimated molecular weight of 5.4kD and a 105bp 3′UTR. Sequence, tissue distribution and phylogenic analysis showed that ssfTZ-f1 belonged to fTZ group, holding highly conserved regions including Ⅰ, Ⅱ and Ⅲ fTZ-f1 boxes and an Af-2 hexamer. Relatively high expression was observed at different larva stages. In juveniles (105 days old), the transcript of ssfTZ-f1 can be detected in all tissues and the abuncance of the gene transcript in testis, ovary, spleen and brain was higher than that in other tissues. In mature fish, the abundance of gene transcript was higher in testis, ovary, spleen and brain than that in liver (trace amount), and the gene was not transcribed in other tissues. The highest abundance of gene transcript was always observed in gonads of both juvenile and mature fish. In addition, the abundance of gene transcript in male tissues were higher than that in female tissue counterparts (P<0.05).

关键词： Sebastes schlegelii fTZ-f1 cloning expression

Theoretical studies of structures and spectroscopic properties of [（tpy）(bpy)RuC≡CC6H4R]+（tpy = 2,2′:6′,2″-terpyridine, bpy = 2,2′-bipyridine;R = f, Cl, H, Me and OMe）

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Science China Technological Sciences 2014年第4期 725-736页

作者： xIA xiQuan zhang Hui zhang GuiLing Key Laboratory of Green Chemical Engineering and Technology of College of Heilongjiang Province College of Chemical and Environmental Engineering Harbin University of Science and Technology

A series of polypyridine ruthenium(II)acetylide complexes,[(tpy)(bpy)RuC≡CC6H4R]+(tpy=2,2′:6′,2″-terpyridine,bpy=2,2′-bipyridine;R=f(1),Cl(2),H(3),Me(4)and OMe(5))are investigated theoretically to explore their electronic structures and spectroscopic *** ground/excited state geometries,electronic structures and spectroscopic properties are first calculated using density functional theory(DfT)and time-dependent DfT(TDDfT).The absorption and emission spectra of the complexes in acetonitrile solution are also obtained by using TDDfT(B3LYP)method associated with the CPCM *** calculations show that the energy levels of HOMOs for 1–5 are sensitive to the substituent on phenylacetylide ligand and increase with the same order of the electron-donating ability of the substituents;however,those of polypyridine-based LUMOs vary *** lowest-energy absorptions and emissions for 1–5 are progressively red-shifted in the order of 1≈2f 1 are attributed to{[dxz(Ru)+π(C≡C)]→[π*(tpy)]}(3MLCT/3LLCT)transition,whereas those of 2–5 are originated from{[dxz/dxy(Ru)+π(C≡C)+π(C6H4R)]→[π*(tpy/bpy)]}(3MLCT/3LLCT)transitions.

关键词： polypyridine ruthenium(II) acetylide complexes absorption spectra emission spectra DfT TDDfT

Acetylcholinesterase inhibitors and antioxidants mining from marine fungi:bioassays,bioactivity coupled LC-MS/MS analyses and molecular networking

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Marine Life Science & Technology 2020年第4期2卷 386-397页

作者： Yingying Nie Wencong Yang Yayue Liu Jingming Yang xiaoling Lei William HGerwick Yi zhang Center for Marine Drugs Research and Development Shenzhen Institute of Guangdong Ocean UniversityShenzhen 518000China College of food Science and Technology Key Laboratory of Guangdong Province Aquatic Products Processing and SafetyGuangdong Province Engineering Laboratory for Marine Biological ProductsGuangdong Provincial Engineering Technology Research Center of Marine FoodKey Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education InstitutionZhanjiang Municipal Key Laboratory of Marine Drugs and Nutrition for Brain HealthResearch Institute for Marine Drugs and NutritionGuangdong Ocean UniversityZhanjiang 52408&China Center for Marine Biotechnology and Biomedicine Scripps Institution of OceanographyUniversity of CaliforniaSan DiegoLa JollaCA 92093USA Collaborative Innovation Center of Seafood Deep Processing Dalian Polytechnic UniversityDalian 116034China

Marine fungi are potentially important resources for bioactive lead compounds for discovering new drugs for diseases such as Alzheimer's *** this paper,the combined bioassay model of acetylcholinesterase(AChE)inhibition,1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical scavenging,and Artemi ci larval lethality was used to evaluate the activity and toxicity of 35 n narine fun gal strains from seas around *** bioactive constitue nfs were revealed by thin layer chromatography(TLC)autography,bioactivity coupled LC-MS/MS and Global Natural Products Social Molecular Networking(GNPS).The results show that the extracts of five strains exhibited higher AChE inhibition ratios than the positive control compound,'tacrine;for which the ratio was 89.8%at 200 pig/*** strains displayed both AChE inhibition(inhibition ratios>20%at 200 pg/ml)and DPPH scavenging activity(scavenging ratios>30%at 200 pg/ml)together with low Artemia larval toxicity(lethal ratesfractioned extracts of four strains contained highly diverse and different bioactive constituents,includi ng strains Talaromyces sp.C2\-\,Aspergillus ter re us C23-3,Trichoderma harzianum DLEN2008005,and Penicillium corylophilum *** the most potent sample f-l 1-1-b(derived from Aspergillus tern us C23-3),five AChE inhibitors and seven antioxida nts were recognized as bioactive molecules by AChE coupled ultrafiltration followed by LC-MS/MS,and LC-MS/MS coupled with DPPH ***,with the aid of GNPS,the AChE inhibitors were plausibly annotated as territrem analogues including territrems A-C/D,arisugacin A and an unknown compound 4,and the seven antioxidants were assigned as butyrolactone *** E,a phenolic derivative and possibly unknown compounds 8-10 and 12.

关键词： Acetylcholinesterase inhibition Antioxida Marine fungi LC-MS/MS Molecular networking

Determination of Boron Trifluoride in Boron Trifluoride Complex by fluoride Ion Selective Electrode

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Transactions of Tianjin University 2016年第1期22卷 83-88页

作者：郎五可张卫江唐银徐姣张雷 School of Chemical Engineering and Technology Tianjin University College of Chemistry and Life Science Weinan Normal University School of Pharmaceutical Engineering Shenyang Pharmaceutical University

A method was proposed to determine boron trifluoride in boron trifluoride complex using fluoride ion selective electrode(ISE). Hydroxide was chosen to mask aluminum for the determination of 0.01—0.1 mol/L of fluoride. The simulation indicated that the permissible aluminum masked at a certain p H value was limited and hardly related to f-concentration and boric acid. It is better to control p H value below 11.5 and the aluminum concentration within 0.025 mol/L to minimize the interference of hydroxide to the fluoride ISE. The decomposition conditions of boron trifluoride by aluminum chloride were investigated. It is found that the f-detection ratio will approach 1.0 if the Al/f molar ratio is 0.3—0.7 and aluminum concentration is no more than 0.02 mol/L when heated at 80 ℃ for 10 min. In one word, hydroxide is quite fit to mask aluminum for samples which contain high content of fluoride and aluminum and the Bf3 content can be successfully determined by this method.

关键词： boron trifluoride complex fluoride ion selective electrode sodium hydroxide aluminum chloride

Neonatal overfeeding in mice aggravates the development of methionine and choline-deficient diet-induced steatohepatitis in adulthood

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Genes & Diseases 2019年第1期6卷 68-77页

作者： Juan Du xuemei Cao Junlin Diao Qijuan zhang Chuan Peng Jibin Li xiaoqiu xiao Laboratory of Lipid&Glucose Metabolism PR China Department of Clinical Nutrition The First Affiliated Hospital of Chongqing Medical UniversityPR China School of Public Health and Management Chongqing Medical UniversityResearch Center for Medicine and Social DevelopmentInnovation Center for Social Risk Governance in HealthChongqing400016PR China

Overfeeding in early life is associated with obesity and insulin resistance in *** the present study,a well-characterized mouse model was used to investigate whether neonatal overfeeding increases susceptibility to the development of non-alcoholic steatohepatitis(NASH)following feeding with a methionine and choline-deficient(MCD)*** overfeeding was induced by adjusting litters to 3 pups per dam(small litter size,SL)in contrast to 10 pups per dam as control(normal litter size,NL).At 11 weeks of age,mice were fed with standard(S)or a methionine and choline-deficient(MCD)diet for 4 *** tolerance tests,tissue staining with haematoxylin and eosin,oil-red O and immunohistochemistry for f4/80,reverse transcription-quantitative polymerase chain reaction(RT-qPCR)and western blotting were *** with NL mice,SL mice exhibited higher body weight gain from 2 weeks of age throughout adulthood,and more profound glucose intolerance as *** regulatory element-binding protein 1c and fatty acid synthase mRNA expression levels in liver were upregulated in SL mice at 3 weeks of *** diet induced typical NASH,especially in SL-MCD mice,evidenced by marked fat accumulation,macrovescular steatosis,ballooned hepatocytes,inflammatory cells infiltration and tumour necrosis factor-a mRNA upregulation in the liver,as well as increased alanine aminotransferase and aspartate aminotransferase levels in the *** were no significant differences in liver fibrosis in all *** during early life exhibited effect with administration of MCD diet in inducing adverse effects on the metabolic function and in promoting the progression of NASH in mice,possibly mediated through dysregulated lipid metabolism in hepatocytes and aggravated hepatic inflammation.

关键词： Inflammation Insulin resistance Neonatal overfeeding Non-alcoholic steatohepatitis Obesity

The red deer antler:Bioinspired design of an Al-Si composite with a fenestrated network-particle structure

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Journal of Materiomics 2020年第3期6卷 545-556页

作者： Zhe Wang Yanjun zhang Song Jiang Jun Wang Dan Du xueliang Wang Yaping Wang School of Materials Science and Engineering Xi’an Polytechnic UniversityXi’an710048China School of Architectural and Surveying Engineering Shanxi Datong UniversityDatong037003China MOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter School of ScienceXi’an Jiaotong UniversityXi’an710049China MOE Key Laboratory of Thermo-fluid Science and Engineering School of Energy and Power EngineeringXi’an Jiaotong UniversityXi’anShaanxi710049China School of Resources Engineering Xi’an University of Architecture and TechnologyXi’anShaanxi710055China

Conventional Al-Si materials always have a coarse and discontinuous Si structure,which limits their application as thermal management ***,nature has evolved efficient strategies to form complex microstructures that exhibit excellent wear resistance and thermal properties;one such example is found in the red deer ***,inspired by the antler structure,AleSi composites with a novel fenestrated network-particle structure(f-N)and with a common isolated island structure(I-I)are designed and ***,the dynamic formation of f-N is tracked and studied using computational fluid dynamics(CfD)*** investigate the reinforcement mechanisms of f-N,the wear resistances and thermal properties of f-N and I-I are compared and *** and experimental results show that the reconstruction of a semi-continuous structure promotes the formation of a striated structure,whereas flowing Si particles provide some sites for the formation of the fenestrated *** dynamic formation of f-N is strongly influenced by the convectionediffusion process and the flow ***,this biomimetic f-N structure exhibits better wear resistance and thermal properties than I-I,owing to its strong structural support and high expansion *** work is expected to provide new perspectives on the microstructural design of thermal management materials with good wear resistance.

关键词： Al-Si materials Dynamic evolution Wear properties Thermal properties Continuity coefficient

Microstructure and properties of Si-TaSi_2 eutectic in situ composite for field emission

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Chinese Science Bulletin 2007年第7期52卷 984-989页

作者： CUI ChunJuan zhang Jun HAN Min CHEN Jun xU NingSheng LIU Lin fU HengZhi State Key Laboratory of Solidification Processing Northwestern Polytechnical University Xi'an 710072 China State Key Laboratory of Optoelectronic Materials and Technology Zhongshan University Guangzhou 510275 China

The Si-TaSi2 eutectic in situ composite for field emission is prepared by electron beam floating zone melting (EBfZM) technique on the basis of Czochralski (CZ) crystal growth technique. The directional solidification microstructure and the field emission properties of the Si-TaSi2 eutectic in situ composite prepared by two kinds of crystal growth techniques have been systematically tested and compared. Researches demonstrated that the solidification microstructure of EBfZM can be fined obviously be-cause of the relatively high solidification rate and very high temperature gradient, i.e. both the diameter and inter-rod spacing of the TaSi2 fibers prepared by EBfZM technique were decreased, and the density and the volume fraction of the TaSi2 fibers prepared by EBfZM technique were increased in comparison with that of the TaSi2 fibers prepared by CZ method. Therefore the field emission property of the Si-TaSi2 eutectic in situ composite prepared by EBfZM can be improved greatly, which exhibits better field emission uniformity and straighter f-N curve.

关键词：硅化钽 Si-TaSi2共晶原位复合材料场发射显示屏显微结构性质

An adjusted ELISpot-based immunoassay for evaluation of SARS-CoV-2-specific T-cell responses

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Biosafety and Health 2022年第3期4卷 179-185页

作者： Hao Lin Jie zhang Shaobo Dong Yaning Liu Peipei Liu George f.Gao William J.Liu Guizhen Wu National Health Commission Key Laboratory of Biosafety Research Units of Adaptive Evolution and Control of Emerging VirusesChinese Academy of Medical SciencesNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention(China CDC)Beijing 102206China Macheng Center for Disease Control and Prevention Huanggang 438300China Center for Biosafety Mega-Science Chinese Academy of SciencesWuhan 430071China

Like antibody evaluation,using an effective antigen‐specific T‐cell immunity assessment method in coronavirus disease 2019(COVID‐19)patients,survivors and vaccinees is crucial for understanding the immune persistence,prognosis assessment,and vaccine development for COVID‐*** study evaluated an empirically adjusted enzyme‐linked immunospot assay for detecting severe acute respiratory syndrome coronavirus 2(SARS‐CoV‐2)‐specific T‐cell immunity in 175 peripheral blood samples from COVID‐19 convalescents and healthy *** of viral nucleic acid were used as the gold standard of infection *** SARS‐CoV‐2M peptide pool had higher sensitivity of 85%and specificity of 71%for the single peptide *** combined peptide pools,the parallel evaluation(at least one of the peptide pools is positive)of total peptide pools(S1&S2&M&N)had higher sensitivity(up to 93%),and the serial evaluation(all peptide pools are positive)of total peptide pools had higher specificity(up to 100%).The result of the serial evaluation was better than that of the parallel evaluation as a *** detection efficiency of M and N peptide pool serial evaluation appeared the highest,with a sensitivity of 80%and specificity of 93%.This T‐cell immunity detection assay introduced in this report can achieve high operability and ***,it can be an effective SARS‐CoV‐2‐specific cellular immune function evaluation method.

关键词： SARS‐CoV‐2 COVID‐19 T‐cell response ELISpot assay Sensitivity Specificity

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俯冲带微量元素分配行为研究:进展和展望

中国科学：地球科学 2020年第12期50卷 1785-1798页

作者：熊小林刘星成李立王锦团陈伟阮梦飞许婷孙众星黄芳芳李建平张磊中国科学院广州地球化学研究所广州510640

微量元素分配对于理解俯冲带地球化学过程及物质循环机制具有重要的意义,文章总结并重点剖析了以下四个方面的俯冲带微量元素分配问题.(1)俯冲板片脱水产生的流体性质和元素迁移:讨论了俯冲带流体和超临界流体的形成以及它们对元素(特... 详细信息

微量元素分配对于理解俯冲带地球化学过程及物质循环机制具有重要的意义,文章总结并重点剖析了以下四个方面的俯冲带微量元素分配问题.(1)俯冲板片脱水产生的流体性质和元素迁移:讨论了俯冲带流体和超临界流体的形成以及它们对元素(特别是高场强元素)的迁移能力和关键控制因素,指出加强超临界流体研究,可能为俯冲带元素迁移过程、机制以及弧岩浆成因等提供新的视角和认识.(2)地幔楔熔融过程中过渡族元素行为:第一排过渡族元素在地幔部分熔融过程中从相容到不相容,价态不变的元素如Sc、Ti、Co、Ni、Zn分配主要取决于残留矿物组合和温度,价态变化的元素如V、fe分配除了受控于矿物组合和温度外,也是氧逸度的函数.因此,过渡族元素的分配行为对示踪地幔楔岩性不均匀性和氧逸度有重要应用.(3)元素分配特异性与弧岩浆演化过程示踪:石榴子石和角闪石对Dy/Yb分配具有特异性,金红石和角闪石对Nb/Ta分配具有特异性;弧岩浆Dy/Yb和Nb/Ta总体随SiO2增加而减小,显示角闪石结晶分异可能最为重要.(4)硫和亲硫元素行为与斑岩成矿:俯冲带Cu、Au等亲硫元素的行为由硫化物和流体控制,由此富水中酸性岩浆体系硫化物稳定性、流体出溶相对硫化物结晶的时机、流体/硫化物质量比率和Cu、Au流体/熔体分配等对理解Cu、Au在岩浆-热液过程中的富集和亏损行为尤为重要.中酸性岩浆主要起源于下地壳弧岩浆分异演化,下地壳中酸性岩浆发生流体出溶以及Cu、Au流体/熔体和流体/硫wj化物分配可能是定量理解Cu、Au如何从深部迁移到浅部成矿的关键所在.

关键词：俯冲带超临界流体过渡族元素元素分配亲硫和成矿元素